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The map label for this gene is phaB [H]

Identifier: 222095943

GI number: 222095943

Start: 2245972

End: 2247027

Strand: Reverse

Name: phaB [H]

Synonym: BCQ_2283

Alternate gene names: 222095943

Gene position: 2247027-2245972 (Counterclockwise)

Preceding gene: 222095948

Following gene: 222095942

Centisome position: 43.09

GC content: 35.13

Gene sequence:

>1056_bases
ATGACTGAACACGTTTTATTTTCTGTTAGCGAAAACGGCGTTGCATCAATTACTTTAAACCGCCCAAAAGCACTTAATTC
TTTATCTTATGACATGTTGCAACCAATTGGGCAAAAACTGAAAGAGTGGGAAAATGATGAGCGAATTGCGCTTATCGTAT
TAAAAGGAGCTGGCACAAAAGGTTTTTGTGCAGGTGGCGATATTAAAACTCTTTATGAAGCACGTTCAAATGAAGCTGCA
TTGCAGCATGCGGAACGTTTCTTTGAAGAAGAGTATGAAATTGATACATATATTTATCAATATAAAAAACCGATTATCGC
TTGTTTAGATGGAATTGTAATGGGTGGTGGTGTCGGTCTTACAAATGGTGCGAAATACCGAATTGTAACTGAGCGTACGA
AATGGGCAATGCCAGAGATGAATATTGGCTTCTTCCCAGACGTCGGTGCTGCTTATTTCTTAAATAAAGCTCCAGGGTAT
GCCGGCCGCTATGTCGCTTTAACAGCATCTATTTTAAAAGCTTCTGACGTATTATTTATAAACGCTGCTGATTATTTTAT
TGCATCTGATTCATTACCAAATTTCCTTACTGAACTGGAAAGTGTAAATTGGTCTAAAGAAGATGATGTACATACTCATT
TAAAAGAAGTCATTCGTACATTTGCAACTGCTCCAACATTAGAAAGCGAGCTTGCTCCTTCATTAGAAGAAATTAATTCA
CATTTTGCATTCGATACAATTGAAGAAATTATTCATTCATTAGAGAAAGATCAAAGTTCCTTTTCTTTAAAAGCGAAAGA
AACGTTATTATCAAAATCTCCTATTTCACTAAAAGTAACATTAAAACAGTTTATTGATGGACAAAATAAGTCCGTTGAAG
AATGTTTCGCTACTGACCTTGTACTTGCTAAAAACTTCATGAGACATGAAGATTTCTTTGAAGGAGTACGATCTGTTGTC
GTTGATAAAGACCAAAATCCAAATTATAAATATAAGCAGTTAAGTGATGTTTCAGAAGAAGATGTAAACCGGTTCTTTAA
CTTACTTAATGCGTAA

Upstream 100 bases:

>100_bases
TAAATTAACATATACCGCCAACTTTAGACAAAATTTATAAAGGACATTTCAGTACGTATAAAGAATTAAGAATAAGAAGA
TATGTAAGGGAGGATGAATT

Downstream 100 bases:

>100_bases
AGTGAGAATTTAATAAGTAGGGTTTATCTCCCTCTTATTATTAGTAGAAGCAATCGCCCTTTCATACGGGATTAAATTGA
GGCTATATAGTCGTATAATT

Product: 3-hydroxyisobutyryl-CoA hydrolase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTEHVLFSVSENGVASITLNRPKALNSLSYDMLQPIGQKLKEWENDERIALIVLKGAGTKGFCAGGDIKTLYEARSNEAA
LQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGLTNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGY
AGRYVALTASILKASDVLFINAADYFIASDSLPNFLTELESVNWSKEDDVHTHLKEVIRTFATAPTLESELAPSLEEINS
HFAFDTIEEIIHSLEKDQSSFSLKAKETLLSKSPISLKVTLKQFIDGQNKSVEECFATDLVLAKNFMRHEDFFEGVRSVV
VDKDQNPNYKYKQLSDVSEEDVNRFFNLLNA

Sequences:

>Translated_351_residues
MTEHVLFSVSENGVASITLNRPKALNSLSYDMLQPIGQKLKEWENDERIALIVLKGAGTKGFCAGGDIKTLYEARSNEAA
LQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGLTNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGY
AGRYVALTASILKASDVLFINAADYFIASDSLPNFLTELESVNWSKEDDVHTHLKEVIRTFATAPTLESELAPSLEEINS
HFAFDTIEEIIHSLEKDQSSFSLKAKETLLSKSPISLKVTLKQFIDGQNKSVEECFATDLVLAKNFMRHEDFFEGVRSVV
VDKDQNPNYKYKQLSDVSEEDVNRFFNLLNA
>Mature_350_residues
TEHVLFSVSENGVASITLNRPKALNSLSYDMLQPIGQKLKEWENDERIALIVLKGAGTKGFCAGGDIKTLYEARSNEAAL
QHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGLTNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGYA
GRYVALTASILKASDVLFINAADYFIASDSLPNFLTELESVNWSKEDDVHTHLKEVIRTFATAPTLESELAPSLEEINSH
FAFDTIEEIIHSLEKDQSSFSLKAKETLLSKSPISLKVTLKQFIDGQNKSVEECFATDLVLAKNFMRHEDFFEGVRSVVV
DKDQNPNYKYKQLSDVSEEDVNRFFNLLNA

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=339, Percent_Identity=40.7079646017699, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI37594469, Length=295, Percent_Identity=39.3220338983051, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI194097323, Length=166, Percent_Identity=33.7349397590361, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI221142681, Length=224, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=23.6434108527132, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787660, Length=174, Percent_Identity=32.7586206896552, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87082183, Length=223, Percent_Identity=23.7668161434978, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI25144157, Length=337, Percent_Identity=37.9821958456973, Blast_Score=241, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI25144160, Length=337, Percent_Identity=37.9821958456973, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17554946, Length=181, Percent_Identity=31.4917127071823, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=168, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=183, Percent_Identity=22.9508196721311, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=178, Percent_Identity=25.2808988764045, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=350, Percent_Identity=32, Blast_Score=169, Evalue=4e-43,
Organism=Drosophila melanogaster, GI28571729, Length=333, Percent_Identity=40.8408408408408, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI28571730, Length=333, Percent_Identity=40.8408408408408, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI20129971, Length=238, Percent_Identity=28.1512605042017, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653477, Length=238, Percent_Identity=28.1512605042017, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653139, Length=147, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 39409; Mature: 39278

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHVLFSVSENGVASITLNRPKALNSLSYDMLQPIGQKLKEWENDERIALIVLKGAGTK
CCCCEEEEECCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
GFCAGGDIKTLYEARSNEAALQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGL
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGYAGRYVALTASILKASDVLFI
CCCCEEEEEECCCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEE
NAADYFIASDSLPNFLTELESVNWSKEDDVHTHLKEVIRTFATAPTLESELAPSLEEINS
ECCCCEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHH
HFAFDTIEEIIHSLEKDQSSFSLKAKETLLSKSPISLKVTLKQFIDGQNKSVEECFATDL
HCCHHHHHHHHHHHHCCCHHCCHHHHHHHHCCCCCEEEEEHHHHHCCCCCCHHHHHHHHH
VLAKNFMRHEDFFEGVRSVVVDKDQNPNYKYKQLSDVSEEDVNRFFNLLNA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TEHVLFSVSENGVASITLNRPKALNSLSYDMLQPIGQKLKEWENDERIALIVLKGAGTK
CCCEEEEECCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
GFCAGGDIKTLYEARSNEAALQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGL
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGYAGRYVALTASILKASDVLFI
CCCCEEEEEECCCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEE
NAADYFIASDSLPNFLTELESVNWSKEDDVHTHLKEVIRTFATAPTLESELAPSLEEINS
ECCCCEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHH
HFAFDTIEEIIHSLEKDQSSFSLKAKETLLSKSPISLKVTLKQFIDGQNKSVEECFATDL
HCCHHHHHHHHHHHHCCCHHCCHHHHHHHHCCCCCEEEEEHHHHHCCCCCCHHHHHHHHH
VLAKNFMRHEDFFEGVRSVVVDKDQNPNYKYKQLSDVSEEDVNRFFNLLNA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]