BacMap

Definition	Bacillus cereus Q1 chromosome, complete genome.
Accession	NC_011969
Length	5,214,195

Click here to switch to the map view.

The map label for this gene is ldh

Identifier: 222095574

GI number: 222095574

Start: 1893712

End: 1894515

Strand: Reverse

Name: ldh

Synonym: BCQ_1914

Alternate gene names: 222095574

Gene position: 1894515-1893712 (Counterclockwise)

Preceding gene: 222095582

Following gene: 222095566

Centisome position: 36.33

GC content: 37.31

Gene sequence:

>804_bases
ATGGATTTAAGTCATGCTGTTCCATTCGCGCCAGCTCCAACTAGAGTATGGAAAGGTAGCTATGAAGATTGTAAAGATGC
TGATCTTGTAGTCATTACGGCTGGATTACCACAAAAGCCAGGCGAAACACGTTTAGATTTAGTTGAGAAAAACGCAAAAA
TCTTTAAACAAATTGTTCGTAGTATTATGGATAGCGGCTTCGATGGTATCTTCTTAATCGCAACTAACCCTGTCGATATT
TTAACTTACGTAACTTGGAAAGAATCTGGTTTACCGAAAGAGCGCGTAATTGGTTCTGGTACAACACTTGATTCTGCTCG
TTTCCGCTATATGTTAGGTGAGTACTTCAATATTGGTCCACACAACATTCACGCTTATATTATCGGCGAACATGGCGATA
CTGAACTTCCAGTTTGGAGCCACGTATCAGTAGGTATTCAAAAACTACAAACACTGCTTGAAAAAGACAACACGTATAAT
CAAGAAGATTTAGACAAAATTTTCATAAACGTTCGTGATGCAGCTTACCATATTATTGAGCGAAAAGGTGCAACTTATTA
TGGTATTGGTATGTCACTTCTACGTGTTACTAAAGCGATTTTAAATGATGAAAATAGTGTATTAACTGTATCGGCTTACT
TAGAAGGTCAATATGGTCAAAAAGATGTGTATATCGGTGTACCTGCTGTTTTAAATCGCGGCGGTGTTCGTGAAATTTTA
GAAGTAGAATTAAGTGAAGACGAAGAATTAAAATTCGATCACTCTGTTCAAGTGTTGAAAGAAACAATGGCTCCTGTTCT
TTAA

Upstream 100 bases:

>100_bases
AGCGGTTGGATGTAGTTATGCTTACTGTATGATTAATCAAGCTGTGGCTGAAGAATTTGTTTTAGTTGATGTAAACGAAG
CAAAAGCTGAAGGGGAAGCA

Downstream 100 bases:

>100_bases
TGATATATGCAAAAAGCAATCCAAAATTTGTTGGATTGCTTTTTACTTTATTTTCTATTTTTTAATCGATTCCTCATTAT
TTACATCAAGTACATCTAAT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 1

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MDLSHAVPFAPAPTRVWKGSYEDCKDADLVVITAGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLIATNPVDI
LTYVTWKESGLPKERVIGSGTTLDSARFRYMLGEYFNIGPHNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYN
QEDLDKIFINVRDAAYHIIERKGATYYGIGMSLLRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREIL
EVELSEDEELKFDHSVQVLKETMAPVL

Sequences:

>Translated_267_residues
MDLSHAVPFAPAPTRVWKGSYEDCKDADLVVITAGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLIATNPVDI
LTYVTWKESGLPKERVIGSGTTLDSARFRYMLGEYFNIGPHNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYN
QEDLDKIFINVRDAAYHIIERKGATYYGIGMSLLRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREIL
EVELSEDEELKFDHSVQVLKETMAPVL
>Mature_267_residues
MDLSHAVPFAPAPTRVWKGSYEDCKDADLVVITAGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLIATNPVDI
LTYVTWKESGLPKERVIGSGTTLDSARFRYMLGEYFNIGPHNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYN
QEDLDKIFINVRDAAYHIIERKGATYYGIGMSLLRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREIL
EVELSEDEELKFDHSVQVLKETMAPVL

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI5031857, Length=261, Percent_Identity=40.6130268199234, Blast_Score=205, Evalue=3e-53,
Organism=Homo sapiens, GI260099723, Length=261, Percent_Identity=40.6130268199234, Blast_Score=205, Evalue=3e-53,
Organism=Homo sapiens, GI47059044, Length=263, Percent_Identity=41.4448669201521, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI221136809, Length=263, Percent_Identity=41.4448669201521, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI291575128, Length=261, Percent_Identity=40.2298850574713, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI4557032, Length=261, Percent_Identity=40.2298850574713, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI9257228, Length=261, Percent_Identity=41.3793103448276, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI4504973, Length=261, Percent_Identity=41.3793103448276, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI15082234, Length=263, Percent_Identity=38.7832699619772, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI207028494, Length=204, Percent_Identity=39.7058823529412, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI260099727, Length=152, Percent_Identity=48.6842105263158, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI260099725, Length=152, Percent_Identity=48.6842105263158, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI103472011, Length=208, Percent_Identity=27.8846153846154, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI103472015, Length=100, Percent_Identity=35, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI21735621, Length=278, Percent_Identity=24.4604316546763, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789632, Length=284, Percent_Identity=27.8169014084507, Blast_Score=74, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17535107, Length=267, Percent_Identity=40.8239700374532, Blast_Score=206, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI17554310, Length=255, Percent_Identity=28.6274509803922, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322765, Length=123, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136226, Length=266, Percent_Identity=39.0977443609023, Blast_Score=204, Evalue=4e-53,
Organism=Drosophila melanogaster, GI45550422, Length=264, Percent_Identity=29.1666666666667, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24647881, Length=241, Percent_Identity=29.045643153527, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24663599, Length=279, Percent_Identity=24.3727598566308, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH1_BACAN (Q81RW4)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_844332.1
- RefSeq:   YP_018568.2
- RefSeq:   YP_028048.1
- ProteinModelPortal:   Q81RW4
- SMR:   Q81RW4
- EnsemblBacteria:   EBBACT00000013196
- EnsemblBacteria:   EBBACT00000013940
- EnsemblBacteria:   EBBACT00000021770
- GeneID:   1086286
- GeneID:   2816222
- GeneID:   2850587
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_1923
- KEGG:   bar:GBAA_1923
- KEGG:   bat:BAS1784
- TIGR:   BA_1923
- TIGR:   GBAA_1923
- GeneTree:   EBGT00050000001153
- HOGENOM:   HBG566126
- OMA:   KVVDSAY
- ProtClustDB:   PRK00066
- BioCyc:   BANT260799:BAS1784-MONOMER
- BioCyc:   BANT261594:GBAA1923-MONOMER
- BRENDA:   1.1.1.27
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 29857; Mature: 29857

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 178-178 BINDING 91-91 BINDING 123-123 BINDING 154-154 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSHAVPFAPAPTRVWKGSYEDCKDADLVVITAGLPQKPGETRLDLVEKNAKIFKQIVR
CCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
SIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGEYFNIGP
HHHHCCCCEEEEEECCCEEEEEEEEECCCCCCHHHEECCCCCCCHHHHHHHHHHHCCCCC
HNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIE
CEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEHHHHHHHHHH
RKGATYYGIGMSLLRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREIL
HCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECHHHHCCCCHHHHH
EVELSEDEELKFDHSVQVLKETMAPVL
EEECCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLSHAVPFAPAPTRVWKGSYEDCKDADLVVITAGLPQKPGETRLDLVEKNAKIFKQIVR
CCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
SIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGEYFNIGP
HHHHCCCCEEEEEECCCEEEEEEEEECCCCCCHHHEECCCCCCCHHHHHHHHHHHCCCCC
HNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIE
CEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEHHHHHHHHHH
RKGATYYGIGMSLLRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREIL
HCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECHHHHCCCCHHHHH
EVELSEDEELKFDHSVQVLKETMAPVL
EEECCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629