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The map label for this gene is hisF [H]

Identifier: 222095143

GI number: 222095143

Start: 1458133

End: 1458891

Strand: Direct

Name: hisF [H]

Synonym: BCQ_1483

Alternate gene names: 222095143

Gene position: 1458133-1458891 (Clockwise)

Preceding gene: 222095142

Following gene: 222095144

Centisome position: 27.96

GC content: 41.24

Gene sequence:

>759_bases
ATGTTAGCGAAACGCATTATTCCATGTCTAGATGTGAAAGAAGGGCGAGTCGTAAAGGGTGTAAATTTTATAGGACTACA
AGATGTCGGGGATCCTGTTGAAATAGCAGCTTTATATAATGATGCGGGAGCAGATGAAATAGTATTTTTAGATATTACGG
CAACGCATGAAGGACGAAAAACAATTATAGATGTTGTAGAAAAAACGGCTTCAAAAGTATTTATTCCTCTTACAGTTGGC
GGAGGAATTTCAAGTGTGAAAGATATGTACAATTTACTAAGAGCGGGAGCAGATAAAGTTTCAATTAACTCAGCAGCAGT
GCGAAATCCAAAATTAATCGAAGAAGGGGCAGAGCATTTTGGCTCACAATGTATTGTCGTCGCAATTGATGCTAGAAAAG
TAGCGGAGGATAAGTGGAATGTATATGTGAACGGCGGAAGAGTTGATACGGGAATTGATGCAATCGGGTGGGCAAAGCGC
GTTACAGAGCTAGGCGCAGGCGAAATTTTATTAACGAGTATGGATGCAGACGGAACGAAAAACGGATATGACCTTCGTTT
AACAGAAGAAATTTCAAAAAGTGTTTCCGTACCAGTCATCGCGTCAGGTGGATGTGGTCATGCTGATCACATTATAGAAG
TGTTTCAAAAGACAACAGTTGATGCAGCGTTAGCGGCATCAATCTTTCATTACGGTGAGGCGACAATAGGGGACGTAAAG
AGAAAGTTAAGAAATGCAAACGTCGAGGTGCGGCTATGA

Upstream 100 bases:

>100_bases
TGAAAAAGTTAAATGATATGAACATATACGGCGTCATAATTGGTAAGGCTCTTTACGAGAAAACGATTGATTTAGAAGAA
GTGCTAGAGGTAACAAAGTT

Downstream 100 bases:

>100_bases
AACCTAACTTTTCAAAAGGACTATTACCAGCAGTTGTAATTGAAGAAGGTACAAAAGAAGTTTTAATGCTAGCTTATATG
AATGAAGAAGCATATGAAAA

Product: imidazole glycerol phosphate synthase subunit hisf

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVEKTASKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAEHFGSQCIVVAIDARKVAEDKWNVYVNGGRVDTGIDAIGWAKR
VTELGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATIGDVK
RKLRNANVEVRL

Sequences:

>Translated_252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVEKTASKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAEHFGSQCIVVAIDARKVAEDKWNVYVNGGRVDTGIDAIGWAKR
VTELGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATIGDVK
RKLRNANVEVRL
>Mature_252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVEKTASKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAEHFGSQCIVVAIDARKVAEDKWNVYVNGGRVDTGIDAIGWAKR
VTELGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATIGDVK
RKLRNANVEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=46.5116279069767, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI87082028, Length=210, Percent_Identity=29.5238095238095, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=33.7579617834395, Blast_Score=155, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26901; Mature: 26901

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCHH
TIIDVVEKTASKVFIPLTVGGGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAEHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHH
GSQCIVVAIDARKVAEDKWNVYVNGGRVDTGIDAIGWAKRVTELGAGEILLTSMDADGTK
CCCEEEEEECCHHHCCCCCEEEEECCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
NGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATIGDVK
CCCEEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RKLRNANVEVRL
HHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCHH
TIIDVVEKTASKVFIPLTVGGGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAEHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHH
GSQCIVVAIDARKVAEDKWNVYVNGGRVDTGIDAIGWAKRVTELGAGEILLTSMDADGTK
CCCEEEEEECCHHHCCCCCEEEEECCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
NGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATIGDVK
CCCEEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RKLRNANVEVRL
HHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA