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The map label for this gene is hisZ [H]

Identifier: 222095137

GI number: 222095137

Start: 1453059

End: 1454321

Strand: Direct

Name: hisZ [H]

Synonym: BCQ_1477

Alternate gene names: 222095137

Gene position: 1453059-1454321 (Clockwise)

Preceding gene: 222095136

Following gene: 222095138

Centisome position: 27.87

GC content: 34.84

Gene sequence:

>1263_bases
ATGACAAAATGGAAACGGGCAAATCCAAATGGAACGAGAGATTATTTATTCGAAGAATGTACGTTAATTGAAGAAGTGGA
GCAAAAATTAAGGCGTACTTTTTTAGAGAGAGGTTATGAAGAAATAAGGACACCAACAATTGAATTTTATGATGTGTTCG
CCTTTCAAAGCAGGCCGATAGATGAAGAAAAGATGTATAAATTTTTTGATGAAAAGGGGAGGATTATCGTATTGCGTCCA
GATATGACAATTCCTTTAGCGAGAGTGATCGGAACGCAGAGGTGTGATACACCACTTAAAGTGACGTATAGCGGAAATGT
ATTTCGAGCGAATGAGTCCCTTACTGGAAAATATAATGAGATCGTGCAAAGTGGTATTGAAATTATCGGTATTGATAATG
TAAGAGCTGAAATTGAATGTGTTATAAGCGTCATTCAATCACTTCAAAAGTTGAAGGTGCAATCTTTTACAATAGAGATT
GGGCAAGTACAGTTATATAAATGTATTGTCAAAAAGCTCTCCATTCATGAGGAAGAAGAGAAAGTTCTTAGAACATATAT
TGAAAGTAAAAATTATGCCGCTTTATCCAATTTTATTAGAGATAAGAAATTAGATCGATGTGATGAAACAGTAAAGTTAC
TGGAGAAATTACCGAGGTTATTTGGGAATTTAGAAGTGATTGAGGAAGCAGAAAAACTCGCCTCCAGTAATGAAATGAAA
ATGGCCATTACAAGAGTGAAAGAAATATATGAAGCAATTGACAAGCTAGGCTATGGATCGTACATATCCATTGATTTAGG
TATGATTCAACATTTGGACTATTATACAGGTGTTATTTTCAAAGGGTATATATATGAAATTGGGGAAGAAATTGTTAGTG
GCGGAAGATATGATGAGTTAATTGGAAATTTTGGAGAAATGTTACCGGCTGTTGGACTCGCGGTGCAAGTTAATCAAATT
GTAAAGGCGCTGCAAGAGCAGCAAAAGCCATATGAACGAAAGAGAATAGATATTATGATTCATTACGAGTTAAATAGATT
AGCAGAAGCGGAACGATTACGAAATTTACTTCAAAAGGACGGGAAAAAAGTAGCGCTATCTTTATTCTCTAATTTAAATG
ATACTTTTCAATTTGCTAGAAAAAATCAAATTGTAACGGTCGTTGAGGCAAAGAACGAATCATTAGTGGAATATGTATGG
AAAGAGAAATGGGTCGTCCAGAAAGAAGGAGAAACTTCATGCGTAACATTCAAATTGCGTTAA

Upstream 100 bases:

>100_bases
AGAGCCTTGTAGGTATATTTTATCTACAAGGCTCTTTTTATTTTCGGTTTAGGTGCATGAAATAAGCATACATTAGAAAG
GGAAAGGTGTGAGAATTTCG

Downstream 100 bases:

>100_bases
CGAAAGGGAGATTAGAAAAACATGTGATTCCGCTCTTTGAGCAGATTGGAATTGATTGTTCAGAGCTCAAAAATAAAGGA
AGAAAACTTGTCTTTCAAAG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTKWKRANPNGTRDYLFEECTLIEEVEQKLRRTFLERGYEEIRTPTIEFYDVFAFQSRPIDEEKMYKFFDEKGRIIVLRP
DMTIPLARVIGTQRCDTPLKVTYSGNVFRANESLTGKYNEIVQSGIEIIGIDNVRAEIECVISVIQSLQKLKVQSFTIEI
GQVQLYKCIVKKLSIHEEEEKVLRTYIESKNYAALSNFIRDKKLDRCDETVKLLEKLPRLFGNLEVIEEAEKLASSNEMK
MAITRVKEIYEAIDKLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDELIGNFGEMLPAVGLAVQVNQI
VKALQEQQKPYERKRIDIMIHYELNRLAEAERLRNLLQKDGKKVALSLFSNLNDTFQFARKNQIVTVVEAKNESLVEYVW
KEKWVVQKEGETSCVTFKLR

Sequences:

>Translated_420_residues
MTKWKRANPNGTRDYLFEECTLIEEVEQKLRRTFLERGYEEIRTPTIEFYDVFAFQSRPIDEEKMYKFFDEKGRIIVLRP
DMTIPLARVIGTQRCDTPLKVTYSGNVFRANESLTGKYNEIVQSGIEIIGIDNVRAEIECVISVIQSLQKLKVQSFTIEI
GQVQLYKCIVKKLSIHEEEEKVLRTYIESKNYAALSNFIRDKKLDRCDETVKLLEKLPRLFGNLEVIEEAEKLASSNEMK
MAITRVKEIYEAIDKLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDELIGNFGEMLPAVGLAVQVNQI
VKALQEQQKPYERKRIDIMIHYELNRLAEAERLRNLLQKDGKKVALSLFSNLNDTFQFARKNQIVTVVEAKNESLVEYVW
KEKWVVQKEGETSCVTFKLR
>Mature_419_residues
TKWKRANPNGTRDYLFEECTLIEEVEQKLRRTFLERGYEEIRTPTIEFYDVFAFQSRPIDEEKMYKFFDEKGRIIVLRPD
MTIPLARVIGTQRCDTPLKVTYSGNVFRANESLTGKYNEIVQSGIEIIGIDNVRAEIECVISVIQSLQKLKVQSFTIEIG
QVQLYKCIVKKLSIHEEEEKVLRTYIESKNYAALSNFIRDKKLDRCDETVKLLEKLPRLFGNLEVIEEAEKLASSNEMKM
AITRVKEIYEAIDKLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDELIGNFGEMLPAVGLAVQVNQIV
KALQEQQKPYERKRIDIMIHYELNRLAEAERLRNLLQKDGKKVALSLFSNLNDTFQFARKNQIVTVVEAKNESLVEYVWK
EKWVVQKEGETSCVTFKLR

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=355, Percent_Identity=25.3521126760563, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI15029520, Length=357, Percent_Identity=25.7703081232493, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71993693, Length=441, Percent_Identity=23.3560090702948, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71993686, Length=441, Percent_Identity=23.3560090702948, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325290, Length=361, Percent_Identity=21.8836565096953, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24643061, Length=451, Percent_Identity=25.7206208425721, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24643059, Length=469, Percent_Identity=24.9466950959488, Blast_Score=111, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 48839; Mature: 48708

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWKRANPNGTRDYLFEECTLIEEVEQKLRRTFLERGYEEIRTPTIEFYDVFAFQSRPI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHEEEHHCCCCC
DEEKMYKFFDEKGRIIVLRPDMTIPLARVIGTQRCDTPLKVTYSGNVFRANESLTGKYNE
CHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCHHHH
IVQSGIEIIGIDNVRAEIECVISVIQSLQKLKVQSFTIEIGQVQLYKCIVKKLSIHEEEE
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHCCHHHH
KVLRTYIESKNYAALSNFIRDKKLDRCDETVKLLEKLPRLFGNLEVIEEAEKLASSNEMK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
MAITRVKEIYEAIDKLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDEL
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IGNFGEMLPAVGLAVQVNQIVKALQEQQKPYERKRIDIMIHYELNRLAEAERLRNLLQKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEEECHHHHHHHHHHHHHHHHC
GKKVALSLFSNLNDTFQFARKNQIVTVVEAKNESLVEYVWKEKWVVQKEGETSCVTFKLR
CHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEECCCCEEEEEEEC
>Mature Secondary Structure 
TKWKRANPNGTRDYLFEECTLIEEVEQKLRRTFLERGYEEIRTPTIEFYDVFAFQSRPI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHEEEHHCCCCC
DEEKMYKFFDEKGRIIVLRPDMTIPLARVIGTQRCDTPLKVTYSGNVFRANESLTGKYNE
CHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCHHHH
IVQSGIEIIGIDNVRAEIECVISVIQSLQKLKVQSFTIEIGQVQLYKCIVKKLSIHEEEE
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHCCHHHH
KVLRTYIESKNYAALSNFIRDKKLDRCDETVKLLEKLPRLFGNLEVIEEAEKLASSNEMK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHH
MAITRVKEIYEAIDKLGYGSYISIDLGMIQHLDYYTGVIFKGYIYEIGEEIVSGGRYDEL
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IGNFGEMLPAVGLAVQVNQIVKALQEQQKPYERKRIDIMIHYELNRLAEAERLRNLLQKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEEECHHHHHHHHHHHHHHHHC
GKKVALSLFSNLNDTFQFARKNQIVTVVEAKNESLVEYVWKEKWVVQKEGETSCVTFKLR
CHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA