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The map label for this gene is nrdE [H]

Identifier: 222095084

GI number: 222095084

Start: 1409179

End: 1410219

Strand: Direct

Name: nrdE [H]

Synonym: BCQ_1424

Alternate gene names: 222095084

Gene position: 1409179-1410219 (Clockwise)

Preceding gene: 222095083

Following gene: 222095085

Centisome position: 27.03

GC content: 37.27

Gene sequence:

>1041_bases
ATGGAAGAAGCTAGAGAGAATACCGTTCATTTTAATAGCGTGGCAGAGCGAATGGAGTATATGAAAGAACATGATTACTA
TTACAACGTTCTGGACGAGTATAACTTGGAGGAAGTAGAAGAGGTATATAACATCGCTTATGGTGAAAACTTCGAGTTCC
AATCTTATATGGCAGCATCTAAGTTCTACAAAGATTATGCGTTAAAAACAAATGATCAAAAACAATACTTAGAAAGCTAT
GAAGATCGTGTAGCAATTGTGTCATTATACTTAGGACGCGGTGATGTTGCGAAGGCAAAACAATTCGCAAGCATGATTGT
CAAACAAAACTACCAACCAGCGACACCAACCTTTTTAAATGCGGGAAGAAGCAGAAGAGGAGAAATGGTGTCTTGTTTCT
TGTTAGAGATGGATGATAGCTTAAATTCAATCGGCTTTAACATTAATACTGCAATGCAATTATCAAAAATCGGTGGTGGA
GTAGCTTTAAACTTATCTAAGCTACGCGCACGCGGTGAGCAAATTAAAGGTATCGACAACGCTGCAAGTGGTGTAGTACC
TGTTATGAAATTACTTGAAGATTCGTTTTCATATGCGAATCAACTTGGCCAACGAAAAGGTGCTGGCGCAGTATACTTAA
ACATTTTCCATTGGGATATTATTGAATTCCTTGATACAAAAAAAATAAATGCCGATGAGAAGAGCCGTATTCAGTCTCTA
TCAATCGGAATTATCGTTCCAAGTAAGTTCTTTGAGCTTGCTGAGAAAAACGAACCTTTCCATGTTTTCGCGCCTTATAC
GGTGTATAAAGAATACGGAAAGCATTTAGATGACATTGATATCGATGAAATGTACGATGAATTAATGAGCAATCCGAAAG
TGAAGAAGAAGCCGCTAGATATTAGTGCACGTGATATGCTTATTAAAATTGCTATGATCCAGCTTGAGTCTGGTTATCCG
TACTTAATGTTTAAATCAAATGCAAATAACCAACATCCATTGAAGGATATTGGAACTGTGAAGATGTCGAACTTGTGTTA
A

Upstream 100 bases:

>100_bases
GTGCGGGAGATTGCGACACATTGAACTGAATAATGAAATCACGCAAATGCAGGACGGTTTTTATCAGCTTCATAAAGATA
AAGAGGCATTAGAAGTCTTT

Downstream 100 bases:

>100_bases
ATATGTAGCACCTTCTAGTAGAAATGCTAGTCGAAAACTCCGTTAAACGGGGAAAGCCTCAAATTTGAGGTAACCTACCG
TGCTAAATCTTATCTTAGAA

Product: ribonucleoside-diphosphate reductase, alpha subunit, n-terminal region

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESY
EDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINTAMQLSKIGGG
VALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIEFLDTKKINADEKSRIQSL
SIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYP
YLMFKSNANNQHPLKDIGTVKMSNLC

Sequences:

>Translated_346_residues
MEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESY
EDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINTAMQLSKIGGG
VALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIEFLDTKKINADEKSRIQSL
SIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYP
YLMFKSNANNQHPLKDIGTVKMSNLC
>Mature_346_residues
MEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESY
EDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINTAMQLSKIGGG
VALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIEFLDTKKINADEKSRIQSL
SIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYP
YLMFKSNANNQHPLKDIGTVKMSNLC

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=307, Percent_Identity=29.3159609120521, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1789030, Length=343, Percent_Identity=48.9795918367347, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI2367133, Length=240, Percent_Identity=22.5, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17555242, Length=307, Percent_Identity=29.3159609120521, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322123, Length=356, Percent_Identity=26.4044943820225, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320914, Length=304, Percent_Identity=27.9605263157895, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17352481, Length=327, Percent_Identity=27.5229357798165, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 39601; Mature: 39601

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGENFEFQSYMAAS
CCCCCCCCCHHHHHHHHHHHHHHCCHHEEHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
KFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLN
HHHHHHHCCCCCHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHH
AGRSRRGEMVSCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDN
CCCCCCCCEEEEEEEEECCCHHHCCCCCHHHHHHHHCCCCEEEEHHHHHHCHHHHCCCCC
AASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIEFLDTKKINADEKSRIQSL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHE
SIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLD
EEEEEECHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCC
ISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLC
CCHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCHHHCCEECCCCC
>Mature Secondary Structure
MEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGENFEFQSYMAAS
CCCCCCCCCHHHHHHHHHHHHHHCCHHEEHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
KFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLN
HHHHHHHCCCCCHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHH
AGRSRRGEMVSCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDN
CCCCCCCCEEEEEEEEECCCHHHCCCCCHHHHHHHHCCCCEEEEHHHHHHCHHHHCCCCC
AASGVVPVMKLLEDSFSYANQLGQRKGAGAVYLNIFHWDIIEFLDTKKINADEKSRIQSL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHE
SIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLD
EEEEEECHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCC
ISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLC
CCHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCHHHCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]