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The map label for this gene is nrdE [H]

Identifier: 222095085

GI number: 222095085

Start: 1410743

End: 1411549

Strand: Direct

Name: nrdE [H]

Synonym: BCQ_1425

Alternate gene names: 222095085

Gene position: 1410743-1411549 (Clockwise)

Preceding gene: 222095084

Following gene: 222095086

Centisome position: 27.06

GC content: 34.82

Gene sequence:

>807_bases
ATGGAAGCTTTAACAGCTGTTTCTGATATGACGATCATTCCGAATGCACCAACTGTGAAAAAAGCAAATGATGAGCTTCA
TTCAGTAGGGCTTGGCGCAATGAACTTACACGGATATTTAGCAAAAAATAAAATCGCTTATGAAAGTGCAGAAGCGAAAG
AGTTCGCTCGTACATTCTTTATGATGTTAAATTACTACTCTATTGAGAAAAGTATGGAAATCGCTAAAGAAAAAGGCGAA
ACATTTAAAGACTTCGATAAATCTGATTATGCGAATGGCACATACTTTGAAAAGTATGAAACGACAGATTACAGCCCAGT
AACTGAAAAAGTTCAGCAATTATTTGAAGGAATTCATATTCCAACAAAAGAAGATTGGACAAGTTTAAAAGAGCAAGTGC
AGAAGAATGGTTTATACAACTCATATCGTCTTGCTATCGCTCCGACACAATCAATCAGTTACGTTCAAAATGCAACTTCA
AGCGTAATGCCGATCGTAAGTCAAATCGAATCAAGAACGTATGCGAATGCGACAACATATTATCCAATGCCGTATTTATC
AAAAGATACGTTCTGGTACTATAAATCTTCTTACGATATGAATCAGTTTAAATTAATTGATTTAATCGCAGAAATTCAAG
AACATATTGACCAAGGTATTAGTACAATTCTTTACGTTAATAGTGATATTTCAACACGTGAATTAGCACGTTACTACATC
TATGCACATAAAAAAGGCTTAAAGAGTCTGTATTATACGAGAACACGTAAATTAAGCGTGGAAGAGTGTGTGGCTTGTAC
CGTTTAA

Upstream 100 bases:

>100_bases
AGATGACATTATCCGTCGTGATATTAACTGTAACTTAGGATCGTTAAATATCGTAAATGTAATGGAAAATAAAGAAATTC
GTGAAGCAGTTCATGCGGGA

Downstream 100 bases:

>100_bases
TGGCATATTGCCATACTAAATGAATAACCCAAAAGTATGAGCAGGGTTATGAACCCTGCTCATACTTGGTTTGTTCTACT
AAACGGTTAGCTTTTCATTA

Product: ribonucleoside-diphosphate reductase alpha chain, c-terminal region

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKEFARTFFMMLNYYSIEKSMEIAKEKGE
TFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIAPTQSISYVQNATS
SVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNSDISTRELARYYI
YAHKKGLKSLYYTRTRKLSVEECVACTV

Sequences:

>Translated_268_residues
MEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKEFARTFFMMLNYYSIEKSMEIAKEKGE
TFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIAPTQSISYVQNATS
SVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNSDISTRELARYYI
YAHKKGLKSLYYTRTRKLSVEECVACTV
>Mature_268_residues
MEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKEFARTFFMMLNYYSIEKSMEIAKEKGE
TFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIAPTQSISYVQNATS
SVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNSDISTRELARYYI
YAHKKGLKSLYYTRTRKLSVEECVACTV

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Escherichia coli, GI1789030, Length=276, Percent_Identity=40.9420289855072, Blast_Score=238, Evalue=2e-64,
Organism=Escherichia coli, GI2367133, Length=267, Percent_Identity=24.7191011235955, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 30846; Mature: 30846

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKEFARTFF
CCCHHHHCCCEECCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
MMLNYYSIEKSMEIAKEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHI
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCC
PTKEDWTSLKEQVQKNGLYNSYRLAIAPTQSISYVQNATSSVMPIVSQIESRTYANATTY
CCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNSDISTRELARYYI
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
YAHKKGLKSLYYTRTRKLSVEECVACTV
HHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKEFARTFF
CCCHHHHCCCEECCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
MMLNYYSIEKSMEIAKEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHI
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCC
PTKEDWTSLKEQVQKNGLYNSYRLAIAPTQSISYVQNATSSVMPIVSQIESRTYANATTY
CCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNSDISTRELARYYI
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
YAHKKGLKSLYYTRTRKLSVEECVACTV
HHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]