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The map label for this gene is potD [H]

Identifier: 222095022

GI number: 222095022

Start: 1351368

End: 1352417

Strand: Direct

Name: potD [H]

Synonym: BCQ_1362

Alternate gene names: 222095022

Gene position: 1351368-1352417 (Clockwise)

Preceding gene: 222095021

Following gene: 222095023

Centisome position: 25.92

GC content: 35.24

Gene sequence:

>1050_bases
ATGAAATTAATGAAAAGATTAGCCGGCGCAGCAATTAGCTTTAGCCTTATTGCTGGTGTACTTGCTGGTTGTGGTGAAAA
AAAAGAAGAGTTAAACATTTATAGCTGGGCTGACAATTTTGATGAGCAAGTGCTAAGAGATTTTGAAAAGAAATATAACG
TGAAAATTAACTATGATAAGTATGCAAGTAATGAAGAAATGCTTGCGAAATTACAAGCTGGTGGCGCAAAGTATGATTTA
ATTCAGCCATCTGATTACATGGTGAAAACAATGGCGAAAATGGATTTATTAGCGCCGTTAGATAAAAAGAATATCCCAAA
TATCGAAAACATGGTTTCTAATTTCAAAACACCTGCGTTTGATCCAGAGAATAAATATTCTTTAGTATATACTTGGGGCG
TAACAGGTATTGCATACAATAAAAAGTATGTGAAGGAAGCACCTACAAGCTGGGCTGACTTATGGAATGAGAAATATAAA
GGGCATGTAACTTTATTAAATGATTCTCGTGAAGTGTTAGGAATGGGTCTTAAGAAGCATGGATTCTCAAACAGTACGAA
AGACGATGCACAGTTAAAGACAGCAGCAACTGATTTACAGAAGCTACTTCCAAACCTATTAGCATTTGATACAGATAATA
TTAAACAAAAATTCATTACAGAAGATGCTTGGATCGGAACAGTTTGGTCTGGAGATGCAGCATTTATCGCAAAAGATAAT
AAAGATGTAGAGTACGTTGTACCAAAAGAAGGCGGCACAATTTGGGCTGATACGTTAGCAATTCCAAAAGGTGCAAAAAA
CAAAGAGCTTGCAGAGAAGTTTATGAACTACTTATTAGATGAAAAAGTAAGTGTGAAAAACTACGAGTCAATTGGATACA
GTAATCCAAATGAAAAAGCTCACCCTCTTCATAGTAAAGAATATCGTGATAACCACATGATCTTCTTAACGAAAGAAGAA
TTAGATCGTACAGAATGGCTTGTTGATGTAGACGATAAGCTAAAAGACTACGACCGTTACTGGACAGAGTTAAAAACAAA
AGGTAAATAA

Upstream 100 bases:

>100_bases
AATCTTCCGTAACAAAGGTGCAGATGGTGAAGAAAACTCTGGAGGACACCTTCCTTTATAATACGAACGATATAGAAAGT
ACGAGGAGGTAATAAAACCG

Downstream 100 bases:

>100_bases
GTAAGAAAATGCGGTTTCTACAGTAGAAATCGCATTTTTTCATAGTAGGAGGAAATCGTTTGAAGAAGTTGCATCAATAT
GAGGTAATGTGTATCGGTTT

Product: spermidine/putrescine ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKLMKRLAGAAISFSLIAGVLAGCGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASNEEMLAKLQAGGAKYDL
IQPSDYMVKTMAKMDLLAPLDKKNIPNIENMVSNFKTPAFDPENKYSLVYTWGVTGIAYNKKYVKEAPTSWADLWNEKYK
GHVTLLNDSREVLGMGLKKHGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDAWIGTVWSGDAAFIAKDN
KDVEYVVPKEGGTIWADTLAIPKGAKNKELAEKFMNYLLDEKVSVKNYESIGYSNPNEKAHPLHSKEYRDNHMIFLTKEE
LDRTEWLVDVDDKLKDYDRYWTELKTKGK

Sequences:

>Translated_349_residues
MKLMKRLAGAAISFSLIAGVLAGCGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASNEEMLAKLQAGGAKYDL
IQPSDYMVKTMAKMDLLAPLDKKNIPNIENMVSNFKTPAFDPENKYSLVYTWGVTGIAYNKKYVKEAPTSWADLWNEKYK
GHVTLLNDSREVLGMGLKKHGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDAWIGTVWSGDAAFIAKDN
KDVEYVVPKEGGTIWADTLAIPKGAKNKELAEKFMNYLLDEKVSVKNYESIGYSNPNEKAHPLHSKEYRDNHMIFLTKEE
LDRTEWLVDVDDKLKDYDRYWTELKTKGK
>Mature_349_residues
MKLMKRLAGAAISFSLIAGVLAGCGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDKYASNEEMLAKLQAGGAKYDL
IQPSDYMVKTMAKMDLLAPLDKKNIPNIENMVSNFKTPAFDPENKYSLVYTWGVTGIAYNKKYVKEAPTSWADLWNEKYK
GHVTLLNDSREVLGMGLKKHGFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDAWIGTVWSGDAAFIAKDN
KDVEYVVPKEGGTIWADTLAIPKGAKNKELAEKFMNYLLDEKVSVKNYESIGYSNPNEKAHPLHSKEYRDNHMIFLTKEE
LDRTEWLVDVDDKLKDYDRYWTELKTKGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=326, Percent_Identity=37.1165644171779, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1787078, Length=364, Percent_Identity=32.1428571428571, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1787711, Length=257, Percent_Identity=26.0700389105058, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39884; Mature: 39884

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLMKRLAGAAISFSLIAGVLAGCGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDK
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHCCEEEEEHH
YASNEEMLAKLQAGGAKYDLIQPSDYMVKTMAKMDLLAPLDKKNIPNIENMVSNFKTPAF
HCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
DPENKYSLVYTWGVTGIAYNKKYVKEAPTSWADLWNEKYKGHVTLLNDSREVLGMGLKKH
CCCCCEEEEEEECCCEEEECHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHCCHHHC
GFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDAWIGTVWSGDAAFIAKDN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCEEEEEECC
KDVEYVVPKEGGTIWADTLAIPKGAKNKELAEKFMNYLLDEKVSVKNYESIGYSNPNEKA
CCEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCC
HPLHSKEYRDNHMIFLTKEELDRTEWLVDVDDKLKDYDRYWTELKTKGK
CCCCCCCCCCCEEEEEEHHHCCCHHEEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLMKRLAGAAISFSLIAGVLAGCGEKKEELNIYSWADNFDEQVLRDFEKKYNVKINYDK
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCEEEECCCCHHHHHHHHHHHHCCEEEEEHH
YASNEEMLAKLQAGGAKYDLIQPSDYMVKTMAKMDLLAPLDKKNIPNIENMVSNFKTPAF
HCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
DPENKYSLVYTWGVTGIAYNKKYVKEAPTSWADLWNEKYKGHVTLLNDSREVLGMGLKKH
CCCCCEEEEEEECCCEEEECHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHCCHHHC
GFSNSTKDDAQLKTAATDLQKLLPNLLAFDTDNIKQKFITEDAWIGTVWSGDAAFIAKDN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCEEEEEECC
KDVEYVVPKEGGTIWADTLAIPKGAKNKELAEKFMNYLLDEKVSVKNYESIGYSNPNEKA
CCEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCC
HPLHSKEYRDNHMIFLTKEELDRTEWLVDVDDKLKDYDRYWTELKTKGK
CCCCCCCCCCCEEEEEEHHHCCCHHEEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]