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The map label for this gene is dagK [H]

Identifier: 222094080

GI number: 222094080

Start: 350768

End: 351529

Strand: Direct

Name: dagK [H]

Synonym: BCQ_0373

Alternate gene names: 222094080

Gene position: 350768-351529 (Clockwise)

Preceding gene: 222094079

Following gene: 222094082

Centisome position: 6.73

GC content: 38.58

Gene sequence:

>762_bases
GTGGCGGCGAGGCAAGCTGTAGAGCGTAAATTTGATGTTGTTATTGCGGCTGGTGGTGACGGCACGTTAAATGAAGTCGT
AAACGGTCTAGTTGGTCATGAATTCCGTCCGAAATTTGGAATTATTCCAGTTGGAACGACAAATGACTTTGCACGTGCAA
TCGGTGTACCTCGTTCTATTGAAGAGGCAGCGGATATTATTTGTGAAGGAAAAACTGTGCCATTAGACCTTGGTAGAGCA
AACGATACATATTTTATTAACATCGCTGGTGGCGGTCGTATTACAGAATTAACATATGAAGTACCAAGTAAGCTAAAGAC
AGTATTAGGACAACTTGCTTATTATTTAAAAGGTATTGAAATGCTACCATCACTACATCCAACATATGTTGAAATTGAGT
ATGATGGAAAATTACTACAAGAAGAAATTACGATGTTTTTAATTACGAATACTCGTTCAGTGGGCGGCTTTGAAAAGGTA
GCACCATATGCATCGATTAACGATGGCTTATTTGATTTATTAGTGCTGAAAAAAGGTTCGATTGCCGACTTAATTAAAGC
TGCAACACAAGCGCAGCGTGGTGAACATATTAACAATCCAAAAGTACTATACACACAAGCGAACCGAATTAAAGTGCATT
CACCAGATAAACTAATGATTAATTTAGATGGTGAATATGGTGGAGATGCACCGATGGAATTCGAAAATATGTATCATTGT
CTGGAACTATTTGTTCCTGAACATCAAGAGGATGCCCTGTAA

Upstream 100 bases:

>100_bases
TGAGCTATTTAAGAAGAGCTTACCGGAAGTATTACAAAAATTAGAACAAGCTGGATATGAAGCATCTTGTCATGCGACAA
CGGGTCCTGGAGACGCAACT

Downstream 100 bases:

>100_bases
AGGCATCCTTTTTTATTTATACATATATGAACGTGTCCAAGGAATGGAATCACTAATCCCTTTTGTAAATACAAATTGAT
GTAAATCAATGTTACCAGTA

Product: lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MAARQAVERKFDVVIAAGGDGTLNEVVNGLVGHEFRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKTVPLDLGRA
NDTYFINIAGGGRITELTYEVPSKLKTVLGQLAYYLKGIEMLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKV
APYASINDGLFDLLVLKKGSIADLIKAATQAQRGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENMYHC
LELFVPEHQEDAL

Sequences:

>Translated_253_residues
MAARQAVERKFDVVIAAGGDGTLNEVVNGLVGHEFRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKTVPLDLGRA
NDTYFINIAGGGRITELTYEVPSKLKTVLGQLAYYLKGIEMLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKV
APYASINDGLFDLLVLKKGSIADLIKAATQAQRGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENMYHC
LELFVPEHQEDAL
>Mature_252_residues
AARQAVERKFDVVIAAGGDGTLNEVVNGLVGHEFRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKTVPLDLGRAN
DTYFINIAGGGRITELTYEVPSKLKTVLGQLAYYLKGIEMLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKVA
PYASINDGLFDLLVLKKGSIADLIKAATQAQRGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENMYHCL
ELFVPEHQEDAL

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=243, Percent_Identity=34.5679012345679, Blast_Score=138, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 27812; Mature: 27681

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARQAVERKFDVVIAAGGDGTLNEVVNGLVGHEFRPKFGIIPVGTTNDFARAIGVPRSI
CCHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCH
EEAADIICEGKTVPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQLAYYLKGIE
HHHHHHEECCCCCEEECCCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
MLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGS
HCCCCCCCEEEEEECCHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCCEEEEEECCCC
IADLIKAATQAQRGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENMYHC
HHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHH
LELFVPEHQEDAL
HHHHCCCCCCCCC
>Mature Secondary Structure 
AARQAVERKFDVVIAAGGDGTLNEVVNGLVGHEFRPKFGIIPVGTTNDFARAIGVPRSI
CHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCH
EEAADIICEGKTVPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQLAYYLKGIE
HHHHHHEECCCCCEEECCCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
MLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGS
HCCCCCCCEEEEEECCHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCCEEEEEECCCC
IADLIKAATQAQRGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENMYHC
HHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHH
LELFVPEHQEDAL
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]