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The map label for this gene is gatB [H]

Identifier: 222094079

GI number: 222094079

Start: 348725

End: 350152

Strand: Direct

Name: gatB [H]

Synonym: BCQ_0372

Alternate gene names: 222094079

Gene position: 348725-350152 (Clockwise)

Preceding gene: 222094078

Following gene: 222094080

Centisome position: 6.69

GC content: 37.32

Gene sequence:

>1428_bases
ATGAATTTAGAAACAATTATTGGTTTAGAGGTTCACGTTGAGTTAAAAACAAATTCGAAAATTTTCTCTGCGAGTCCAAC
AGAATTCGGAGCGGAGCCAAATACACAAACAAGTGTAATTGACTTAGGATACCCAGGGGTACTTCCTACTTTAAATAAAG
AAGCAGTTAACTTTGCAATGAAAGCTGCAATGGCATTAAACTGTGAAATCGCAACGGAAACGAAGTTCGACCGTAAAAAC
TATTTCTACCCAGATAATCCGAAAGCTTACCAAATTTCTCAATTTGATAAGCCAATTGGTGAAAATGGCTGGATTGAAAT
CGAAGTAGACGGTAAAAAGAAACGTATCGGTATTACACGTCTTCATTTAGAAGAAGATGCTGGTAAATCAACGCATACAG
CTGATGGTTCATTAGTAGACTACAATCGTCAAGGTATGCCTTTAATCGAGATCGTGTCTGAGCCAGATATGCGTACACCG
GAAGAAGCATATGCATACTTAGAGAAGTTAAAATCAATTATTCAATACACGGGCGTATCTGACTGTAAGATGGAAGAAGG
TTCCTTGCGTTGTGATGCGAACATTTCTCTTCGTCCAGTTGGACAAGAAAAGTTCGGTACAAAAGCAGAACTGAAAAACT
TAAACTCATTCACTTACGTACAAAAAGGTCTTGAGCACGAGCAAGTGCGTCAAGAAAAAGAATTATTATCTGGTGGTATC
ATCCAACAAGAAACACGTCGTTATGATGAAGCAACGAAGAAAACAATCTTAATGCGTGTGAAAGAAGGATCTGATGATTA
CCGTTACTTCCCAGAACCAGACTTAGTTGAACTTTACATCGACGATGAGTGGAAAGAAGCAGTTCGTGCTTCTATTCCAG
AACTTCCAGATGCACGTAAAGCTCGCTACGTTGCAGAATTAGGCTTACCAGCTTATGATGCACACGTATTAACATTAACG
AAAGAAATGTCTGATTTCTTTGAAGCAACTGTTGCAGACGGTGCTGATGCGAAATTAACATCAAACTGGTTAATGGGTGA
AGTACTTGCATACTTAAACAAACAACAAAAAGAATTAAAAGATGTTGCATTAACGCCTGCTGGTTTATCTAAAATGGTTC
AATTAATTGAAAAAGGTACAATTTCTTCTAAAATTGCGAAGAAAGTATTTAACGAATTAATTGAAAAAGGTGGAGACCCA
GAGGAAATCGTTAAAGCGAAAGGTCTTGTTCAAATTTCTGACGAGGGTACACTTCGTAAAGTTGTAACAGAAATTCTTGA
TAATAATGAGCAATCTATCGAAGACTTTAAAAACGGTAAAGACCGTGCGATTGGCTTCTTAGTTGGTCAAATTATGAAAG
CTACAAAAGGACAAGCAAATCCACCGCTTGTTAACAAAATCTTACTTGAAGAGATTAATAAGCGATAA

Upstream 100 bases:

>100_bases
AACACTTCGATGAAGCGACAATTTACCGCGTTGCACATGCGTTTGAGCAAGCAACAGACTATCATACAAAAAAAGCAAGT
CTGTAAGGAGGCGAGCATAG

Downstream 100 bases:

>100_bases
TAGTAGTAACTTTTAAAGATTAAGCAGAAAAACACCCGTACATGGGTGTTTTTCTAGGTTAATCAAACGTTTGTTTAAAT
GAGAGAAAATCATTTAAACA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit b

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNLETIIGLEVHVELKTNSKIFSASPTEFGAEPNTQTSVIDLGYPGVLPTLNKEAVNFAMKAAMALNCEIATETKFDRKN
YFYPDNPKAYQISQFDKPIGENGWIEIEVDGKKKRIGITRLHLEEDAGKSTHTADGSLVDYNRQGMPLIEIVSEPDMRTP
EEAYAYLEKLKSIIQYTGVSDCKMEEGSLRCDANISLRPVGQEKFGTKAELKNLNSFTYVQKGLEHEQVRQEKELLSGGI
IQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDDEWKEAVRASIPELPDARKARYVAELGLPAYDAHVLTLT
KEMSDFFEATVADGADAKLTSNWLMGEVLAYLNKQQKELKDVALTPAGLSKMVQLIEKGTISSKIAKKVFNELIEKGGDP
EEIVKAKGLVQISDEGTLRKVVTEILDNNEQSIEDFKNGKDRAIGFLVGQIMKATKGQANPPLVNKILLEEINKR

Sequences:

>Translated_475_residues
MNLETIIGLEVHVELKTNSKIFSASPTEFGAEPNTQTSVIDLGYPGVLPTLNKEAVNFAMKAAMALNCEIATETKFDRKN
YFYPDNPKAYQISQFDKPIGENGWIEIEVDGKKKRIGITRLHLEEDAGKSTHTADGSLVDYNRQGMPLIEIVSEPDMRTP
EEAYAYLEKLKSIIQYTGVSDCKMEEGSLRCDANISLRPVGQEKFGTKAELKNLNSFTYVQKGLEHEQVRQEKELLSGGI
IQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDDEWKEAVRASIPELPDARKARYVAELGLPAYDAHVLTLT
KEMSDFFEATVADGADAKLTSNWLMGEVLAYLNKQQKELKDVALTPAGLSKMVQLIEKGTISSKIAKKVFNELIEKGGDP
EEIVKAKGLVQISDEGTLRKVVTEILDNNEQSIEDFKNGKDRAIGFLVGQIMKATKGQANPPLVNKILLEEINKR
>Mature_475_residues
MNLETIIGLEVHVELKTNSKIFSASPTEFGAEPNTQTSVIDLGYPGVLPTLNKEAVNFAMKAAMALNCEIATETKFDRKN
YFYPDNPKAYQISQFDKPIGENGWIEIEVDGKKKRIGITRLHLEEDAGKSTHTADGSLVDYNRQGMPLIEIVSEPDMRTP
EEAYAYLEKLKSIIQYTGVSDCKMEEGSLRCDANISLRPVGQEKFGTKAELKNLNSFTYVQKGLEHEQVRQEKELLSGGI
IQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDDEWKEAVRASIPELPDARKARYVAELGLPAYDAHVLTLT
KEMSDFFEATVADGADAKLTSNWLMGEVLAYLNKQQKELKDVALTPAGLSKMVQLIEKGTISSKIAKKVFNELIEKGGDP
EEIVKAKGLVQISDEGTLRKVVTEILDNNEQSIEDFKNGKDRAIGFLVGQIMKATKGQANPPLVNKILLEEINKR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=489, Percent_Identity=37.2188139059305, Blast_Score=311, Evalue=8e-85,
Organism=Caenorhabditis elegans, GI115534094, Length=296, Percent_Identity=39.1891891891892, Blast_Score=214, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=515, Percent_Identity=29.9029126213592, Blast_Score=221, Evalue=1e-58,
Organism=Drosophila melanogaster, GI45550808, Length=484, Percent_Identity=32.8512396694215, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.7

Molecular weight: Translated: 53238; Mature: 53238

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLETIIGLEVHVELKTNSKIFSASPTEFGAEPNTQTSVIDLGYPGVLPTLNKEAVNFAM
CCCCEEEEEEEEEEEECCCEEEECCCHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
KAAMALNCEIATETKFDRKNYFYPDNPKAYQISQFDKPIGENGWIEIEVDGKKKRIGITR
HHHHHEEEEEECCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCEEEEEECCCEEEECEEE
LHLEEDAGKSTHTADGSLVDYNRQGMPLIEIVSEPDMRTPEEAYAYLEKLKSIIQYTGVS
EEEECCCCCCCCCCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
DCKMEEGSLRCDANISLRPVGQEKFGTKAELKNLNSFTYVQKGLEHEQVRQEKELLSGGI
CCEECCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCCC
IQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDDEWKEAVRASIPELPDARK
HHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCHHH
ARYVAELGLPAYDAHVLTLTKEMSDFFEATVADGADAKLTSNWLMGEVLAYLNKQQKELK
HHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DVALTPAGLSKMVQLIEKGTISSKIAKKVFNELIEKGGDPEEIVKAKGLVQISDEGTLRK
HHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCHHHH
VVTEILDNNEQSIEDFKNGKDRAIGFLVGQIMKATKGQANPPLVNKILLEEINKR
HHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLETIIGLEVHVELKTNSKIFSASPTEFGAEPNTQTSVIDLGYPGVLPTLNKEAVNFAM
CCCCEEEEEEEEEEEECCCEEEECCCHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
KAAMALNCEIATETKFDRKNYFYPDNPKAYQISQFDKPIGENGWIEIEVDGKKKRIGITR
HHHHHEEEEEECCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCEEEEEECCCEEEECEEE
LHLEEDAGKSTHTADGSLVDYNRQGMPLIEIVSEPDMRTPEEAYAYLEKLKSIIQYTGVS
EEEECCCCCCCCCCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
DCKMEEGSLRCDANISLRPVGQEKFGTKAELKNLNSFTYVQKGLEHEQVRQEKELLSGGI
CCEECCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHCCC
IQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDDEWKEAVRASIPELPDARK
HHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCHHH
ARYVAELGLPAYDAHVLTLTKEMSDFFEATVADGADAKLTSNWLMGEVLAYLNKQQKELK
HHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DVALTPAGLSKMVQLIEKGTISSKIAKKVFNELIEKGGDPEEIVKAKGLVQISDEGTLRK
HHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCHHHH
VVTEILDNNEQSIEDFKNGKDRAIGFLVGQIMKATKGQANPPLVNKILLEEINKR
HHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA