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The map label for this gene is miaA

Identifier: 221636275

GI number: 221636275

Start: 849676

End: 850665

Strand: Reverse

Name: miaA

Synonym: trd_A0869

Alternate gene names: 221636275

Gene position: 850665-849676 (Counterclockwise)

Preceding gene: 221636279

Following gene: 221636274

Centisome position: 92.69

GC content: 64.65

Gene sequence:

>990_bases
ATGAACAAGCGCAAACCGGTGGTCGCGCTCGTGGGTCCGACCTCGGTCGGGAAGACAGCGACCGCGATCGAGTTGGCATT
GACATTCGGGGGGGAAGTCGTCTCAGCCGATTCACGGTATCTCTACCGCGGCATGGATATCGGTACGGACAAGCCGACGC
TGGAGGAACGCCGGGGGGTTCCCCATCATCTCATCGACATTCTCGATCCGCGCGACGACTACTCGCTGGCTCTGTTCCAA
CGTGATGCCCAGCGGGCGATCGAGGAGATCCATGCGCGTGGTCGGCTGCCGATCGTGGCCGGCGGGACACCGCTGTATCT
GCGTGCACTGCTGGAGGGGTGGCGCATTCCACCTGCTCCACCCAATCCGGAGTTGCGCGCCCAGCTGGAGTTGCGTGCCC
GCCGGGAGGGGCCGGAGGCGTTGCATCGGGAGCTCCAGACAGTGGATCCGGTCGCGGCAGCCCGCATTCCCCCAGAGAAC
GTCCGTCGAGTTATCCGCGCACTCGAGATCTTCCTGACGACCGGGAGACGGATGACCGAACTGGAAGGCCGAGAAGCACC
GCCCTGGCGTGTGCTGTGGCTCGGCCTGACGATGCCGCGCGACGAACTGTACCGCCGGATCGACGAGCGAGTGGATCGGC
AGGTCGCGCGCGGCCTGGTGGAAGAGGTGCAGCGCTTGCTGGAGCAGGGAGTGCCGCCGGACGCACCCGCGATGACGGCG
CTCGGTTATCGGCAGATCGTCGCGTTTCTTACCGGGCAGCTGAGCTTGGAGGAAGCGATCCAGCGGATCAAGTACGATAC
GCACCGGTATGCGCGGCATCAGCTGACCTGGTTGCGGCGGATGAAGCAGGTGGAGTGGTACGACGTGACCCAACCAGGTT
GGTACGAGCAGTTGCGCGAGCGTGTCGAAAGGTCCCTCCGGGAAGAATCCGATGAAGGGGATGTTGCCGTACACCAGAGC
GGAGGAGGAAAGGAAGCGCCTCGCGCGTGA

Upstream 100 bases:

>100_bases
GGGCTTGTACCATCACGCGGAGCACGCGGGTGAGCCTCCTGCCAGCGCTGGACGTAACGGGTCGCGCGACCGGCCGAGAG
TCTACCAGGAGTCGAGGCAG

Downstream 100 bases:

>100_bases
GGAGGAGGCACGGAAGCGGCCCAGCTGGGCCGCTTCCGTGTTTTCGTCGGCTGCATGCTCGAACGACCGACGATGGCCCA
CCACGCTGGAGAGGGAGAGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNKRKPVVALVGPTSVGKTATAIELALTFGGEVVSADSRYLYRGMDIGTDKPTLEERRGVPHHLIDILDPRDDYSLALFQ
RDAQRAIEEIHARGRLPIVAGGTPLYLRALLEGWRIPPAPPNPELRAQLELRARREGPEALHRELQTVDPVAAARIPPEN
VRRVIRALEIFLTTGRRMTELEGREAPPWRVLWLGLTMPRDELYRRIDERVDRQVARGLVEEVQRLLEQGVPPDAPAMTA
LGYRQIVAFLTGQLSLEEAIQRIKYDTHRYARHQLTWLRRMKQVEWYDVTQPGWYEQLRERVERSLREESDEGDVAVHQS
GGGKEAPRA

Sequences:

>Translated_329_residues
MNKRKPVVALVGPTSVGKTATAIELALTFGGEVVSADSRYLYRGMDIGTDKPTLEERRGVPHHLIDILDPRDDYSLALFQ
RDAQRAIEEIHARGRLPIVAGGTPLYLRALLEGWRIPPAPPNPELRAQLELRARREGPEALHRELQTVDPVAAARIPPEN
VRRVIRALEIFLTTGRRMTELEGREAPPWRVLWLGLTMPRDELYRRIDERVDRQVARGLVEEVQRLLEQGVPPDAPAMTA
LGYRQIVAFLTGQLSLEEAIQRIKYDTHRYARHQLTWLRRMKQVEWYDVTQPGWYEQLRERVERSLREESDEGDVAVHQS
GGGKEAPRA
>Mature_329_residues
MNKRKPVVALVGPTSVGKTATAIELALTFGGEVVSADSRYLYRGMDIGTDKPTLEERRGVPHHLIDILDPRDDYSLALFQ
RDAQRAIEEIHARGRLPIVAGGTPLYLRALLEGWRIPPAPPNPELRAQLELRARREGPEALHRELQTVDPVAAARIPPEN
VRRVIRALEIFLTTGRRMTELEGREAPPWRVLWLGLTMPRDELYRRIDERVDRQVARGLVEEVQRLLEQGVPPDAPAMTA
LGYRQIVAFLTGQLSLEEAIQRIKYDTHRYARHQLTWLRRMKQVEWYDVTQPGWYEQLRERVERSLREESDEGDVAVHQS
GGGKEAPRA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=31.629392971246, Blast_Score=145, Evalue=6e-35,
Organism=Escherichia coli, GI1790613, Length=300, Percent_Identity=43.6666666666667, Blast_Score=228, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=25.9136212624585, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=296, Percent_Identity=33.445945945946, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24649830, Length=279, Percent_Identity=31.5412186379928, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_THERP (B9L505)

Other databases:

- EMBL:   CP001276
- RefSeq:   YP_002524151.1
- ProteinModelPortal:   B9L505
- GeneID:   7357493
- GenomeReviews:   CP001276_GR
- KEGG:   tro:trd_A0869
- OMA:   VNADSMQ
- ProtClustDB:   CLSK2808465
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 37581; Mature: 37581

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRKPVVALVGPTSVGKTATAIELALTFGGEVVSADSRYLYRGMDIGTDKPTLEERRGV
CCCCCCEEEEECCCCCCCHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCCCCHHHHCCC
PHHLIDILDPRDDYSLALFQRDAQRAIEEIHARGRLPIVAGGTPLYLRALLEGWRIPPAP
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCC
PNPELRAQLELRARREGPEALHRELQTVDPVAAARIPPENVRRVIRALEIFLTTGRRMTE
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
LEGREAPPWRVLWLGLTMPRDELYRRIDERVDRQVARGLVEEVQRLLEQGVPPDAPAMTA
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LGYRQIVAFLTGQLSLEEAIQRIKYDTHRYARHQLTWLRRMKQVEWYDVTQPGWYEQLRE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHH
RVERSLREESDEGDVAVHQSGGGKEAPRA
HHHHHHHCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MNKRKPVVALVGPTSVGKTATAIELALTFGGEVVSADSRYLYRGMDIGTDKPTLEERRGV
CCCCCCEEEEECCCCCCCHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCCCCHHHHCCC
PHHLIDILDPRDDYSLALFQRDAQRAIEEIHARGRLPIVAGGTPLYLRALLEGWRIPPAP
CHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCC
PNPELRAQLELRARREGPEALHRELQTVDPVAAARIPPENVRRVIRALEIFLTTGRRMTE
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
LEGREAPPWRVLWLGLTMPRDELYRRIDERVDRQVARGLVEEVQRLLEQGVPPDAPAMTA
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LGYRQIVAFLTGQLSLEEAIQRIKYDTHRYARHQLTWLRRMKQVEWYDVTQPGWYEQLRE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHH
RVERSLREESDEGDVAVHQSGGGKEAPRA
HHHHHHHCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA