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The map label for this gene is ppsA [H]

Identifier: 221636185

GI number: 221636185

Start: 761715

End: 762797

Strand: Reverse

Name: ppsA [H]

Synonym: trd_A0779

Alternate gene names: 221636185

Gene position: 762797-761715 (Counterclockwise)

Preceding gene: 221636186

Following gene: 221636183

Centisome position: 83.12

GC content: 65.56

Gene sequence:

>1083_bases
ATGCGCCGATCACCATTCGTCCGCGAGCTCACTACCCTCGGCCATGCCGATCGGCCCCTGGTGGGGGGTAAGGCAGCGAG
TCTGGGGATGCTCCTGGCGGCTGGCTGTCCGGTTCCATCTGGCTTCGCTCTGACGACCGAGGCCTACCGGTACTTTCTGG
AGCGGAACGGTCTGACCGGTCTCTTCGAGTTCGTACAGGATGAGGAGAAGCGATTGGACGATGCAGCGACGCAGCTCTCG
CGCCGCGTCGAGGAGGCGTTCCTCGCAGCGGCGTTGCCGGAGGATTTGGCCGATGAGCTCGCCGAGTCGTATCTCGAACT
CGGGAAAGAGATCGGCCAGCCGGAGCCGGTGGTCGCGGTTCGCTCGAGTGCGGCAGATGAGGACAGCCGTACGGCGAGCT
TTGCAGGGCAACACGAGACCTATCTCGGTATCCAGGGGATCGACTCACTGTTCCGAGCAGTGCGGCGTTGCTGGGCCAGC
GCCTACACGCCGCGGGCGATCGCCTATCGCGTCCACCGTGGCCTCTCCTTGGCGGACGCTTCGGTCGGCGTCGTGGTGCA
GCTGCTGGTCGAGCCGCGCGCGGCTGGAGTGCTCTTCACGCTCAGCCCGCGCACGGGCGATCGCTCGCTCATCGTGATCG
AAGGCAGTTGGGGGCTCGGTCAGGCGGTCGTCGCTGGCGAGGTCACGCCCGATGAGTACGTGGTCAGCAAGGTGACGCTC
GAACTGGTGCGCAAACAGGTCAATGCGAAGCCGTTCCGGTATCGGCGTGGGGGATCGGATGGCTCGCTGGTCCGTGAAGA
ACTTCCGCCCGATCTCGCCAGCGCACCCTGTCTCTTGGAGTCCGAGGTGATCGAGCTTGCCCGGCGCGCTCGCGAACTCG
AGAAGCGACTGGGCTATCCCCTCGATGTGGAGTGGGCGATGTGCGATCAGCCGGAACGCGGAACGATCTTCTTCCTGCAA
TGCCGGCCGGAGACGGTATGGACTACCAAGGAGCGACCGCTCGCGACCCTGGCGGGGAGCAACCCGATCATGGCCATCGC
GCAGACGCTGGTACGCTCGCCGAGCCGTCCTGGGAACGGATGA

Upstream 100 bases:

>100_bases
AGGATCCGGACAGGGCAGTACCTGCGAGTCGATGGCGAGAACGGAGTCGTCACCATTCTCGACTGACCAATGGCGAGGTC
AGGAAGGAGAACGGTTTCCG

Downstream 100 bases:

>100_bases
GGGCGCCTTCGGAACGATCAAACGATCGAGTGTCGGGTGGACTCTCGAAGCTCGGTGGCGAACCGGCAGACCGAGCGGGT
ACTAGGTGGGGTGCGCAGTA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MRRSPFVRELTTLGHADRPLVGGKAASLGMLLAAGCPVPSGFALTTEAYRYFLERNGLTGLFEFVQDEEKRLDDAATQLS
RRVEEAFLAAALPEDLADELAESYLELGKEIGQPEPVVAVRSSAADEDSRTASFAGQHETYLGIQGIDSLFRAVRRCWAS
AYTPRAIAYRVHRGLSLADASVGVVVQLLVEPRAAGVLFTLSPRTGDRSLIVIEGSWGLGQAVVAGEVTPDEYVVSKVTL
ELVRKQVNAKPFRYRRGGSDGSLVREELPPDLASAPCLLESEVIELARRARELEKRLGYPLDVEWAMCDQPERGTIFFLQ
CRPETVWTTKERPLATLAGSNPIMAIAQTLVRSPSRPGNG

Sequences:

>Translated_360_residues
MRRSPFVRELTTLGHADRPLVGGKAASLGMLLAAGCPVPSGFALTTEAYRYFLERNGLTGLFEFVQDEEKRLDDAATQLS
RRVEEAFLAAALPEDLADELAESYLELGKEIGQPEPVVAVRSSAADEDSRTASFAGQHETYLGIQGIDSLFRAVRRCWAS
AYTPRAIAYRVHRGLSLADASVGVVVQLLVEPRAAGVLFTLSPRTGDRSLIVIEGSWGLGQAVVAGEVTPDEYVVSKVTL
ELVRKQVNAKPFRYRRGGSDGSLVREELPPDLASAPCLLESEVIELARRARELEKRLGYPLDVEWAMCDQPERGTIFFLQ
CRPETVWTTKERPLATLAGSNPIMAIAQTLVRSPSRPGNG
>Mature_360_residues
MRRSPFVRELTTLGHADRPLVGGKAASLGMLLAAGCPVPSGFALTTEAYRYFLERNGLTGLFEFVQDEEKRLDDAATQLS
RRVEEAFLAAALPEDLADELAESYLELGKEIGQPEPVVAVRSSAADEDSRTASFAGQHETYLGIQGIDSLFRAVRRCWAS
AYTPRAIAYRVHRGLSLADASVGVVVQLLVEPRAAGVLFTLSPRTGDRSLIVIEGSWGLGQAVVAGEVTPDEYVVSKVTL
ELVRKQVNAKPFRYRRGGSDGSLVREELPPDLASAPCLLESEVIELARRARELEKRLGYPLDVEWAMCDQPERGTIFFLQ
CRPETVWTTKERPLATLAGSNPIMAIAQTLVRSPSRPGNG

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=341, Percent_Identity=35.7771260997067, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17564524, Length=302, Percent_Identity=29.4701986754967, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 39312; Mature: 39312

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSPFVRELTTLGHADRPLVGGKAASLGMLLAAGCPVPSGFALTTEAYRYFLERNGLTG
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHH
LFEFVQDEEKRLDDAATQLSRRVEEAFLAAALPEDLADELAESYLELGKEIGQPEPVVAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
RSSAADEDSRTASFAGQHETYLGIQGIDSLFRAVRRCWASAYTPRAIAYRVHRGLSLADA
ECCCCCCCCCHHHHCCCCCCEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHH
SVGVVVQLLVEPRAAGVLFTLSPRTGDRSLIVIEGSWGLGQAVVAGEVTPDEYVVSKVTL
HHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCHHHHHHHHHH
ELVRKQVNAKPFRYRRGGSDGSLVREELPPDLASAPCLLESEVIELARRARELEKRLGYP
HHHHHHHCCCCCEEECCCCCCCHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
LDVEWAMCDQPERGTIFFLQCRPETVWTTKERPLATLAGSNPIMAIAQTLVRSPSRPGNG
CCCCCCCCCCCCCCCEEEEEECCCCEECCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRRSPFVRELTTLGHADRPLVGGKAASLGMLLAAGCPVPSGFALTTEAYRYFLERNGLTG
CCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHH
LFEFVQDEEKRLDDAATQLSRRVEEAFLAAALPEDLADELAESYLELGKEIGQPEPVVAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEE
RSSAADEDSRTASFAGQHETYLGIQGIDSLFRAVRRCWASAYTPRAIAYRVHRGLSLADA
ECCCCCCCCCHHHHCCCCCCEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHH
SVGVVVQLLVEPRAAGVLFTLSPRTGDRSLIVIEGSWGLGQAVVAGEVTPDEYVVSKVTL
HHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCHHHHHHHHHH
ELVRKQVNAKPFRYRRGGSDGSLVREELPPDLASAPCLLESEVIELARRARELEKRLGYP
HHHHHHHCCCCCEEECCCCCCCHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
LDVEWAMCDQPERGTIFFLQCRPETVWTTKERPLATLAGSNPIMAIAQTLVRSPSRPGNG
CCCCCCCCCCCCCCCEEEEEECCCCEECCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7805870 [H]