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The map label for this gene is rutB [H]

Identifier: 221635948

GI number: 221635948

Start: 538092

End: 538706

Strand: Reverse

Name: rutB [H]

Synonym: trd_A0542

Alternate gene names: 221635948

Gene position: 538706-538092 (Counterclockwise)

Preceding gene: 221635951

Following gene: 221635947

Centisome position: 58.7

GC content: 60.65

Gene sequence:

>615_bases
ATGCCGCGAACAGTCGAAGTCCCGGAGTACACGATCGCACCCGAAGTGGAGATCGACCCCCGCACCACGGCGCTGATCGT
CGTCGATATGCAAAACGATTTCGTTCGGCCAGAAGGGAAATTGTTCGTTCCGGATGCACCGGCAACCGTACCCAAGATCC
AAGCATTGCTCGCTTTCGCCCGCCAGCACGGGATTTTCACTGTCTTCACGCAAGACACTCATTATCCAGGTGACCCGGAG
TTTCCCATCTGGGGTGAACATTGCGTGGCGGGGACCTGGGGCTGGCAAATCATCGATGAACTGGCGCCACGGGAAGGAGA
ACTCGTGCTGCAAAAGCGGCGTTACGATGCGTTCTACGGGACGCCGCTGGATCACGAGCTCCGCTTGCGCAAGATCGAAC
AACTCATCATCTGTGGGACCGTGGCCTCGATCTGCGTGCACTATACGGCAGCGAGCGCAGCACTGCGCTGGTATGGCGTG
ATCATTCCAGTGGATGCGACGTCGGCGCTCCATCCCTTCGATCTGGAAGCAGCCAACCGGCAGACCGCGTTCCTGTTCCG
GGGCGTTCTGACCCGAGCGGACGGCGTGCGGCTGCGGAGCGGCGTACCGGCGTAG

Upstream 100 bases:

>100_bases
TGGAACCACGAACCGGGTGCGATGCATCATTAGTGGTGCAGATCGTCAGGCCAAGCTCCGTTGACTGGTCGGTGCTGCGT
ATCGGAGTGGGAGGGAGACC

Downstream 100 bases:

>100_bases
ACGCATTGACCCCTTGACAACGAGGCCTCGGCTGAGTAGTATCTGACAACGCTGCGCGAGCGGGCGCAGCCCGGGAACCG
GCGGAAGGGCTTGACAGCAT

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MPRTVEVPEYTIAPEVEIDPRTTALIVVDMQNDFVRPEGKLFVPDAPATVPKIQALLAFARQHGIFTVFTQDTHYPGDPE
FPIWGEHCVAGTWGWQIIDELAPREGELVLQKRRYDAFYGTPLDHELRLRKIEQLIICGTVASICVHYTAASAALRWYGV
IIPVDATSALHPFDLEAANRQTAFLFRGVLTRADGVRLRSGVPA

Sequences:

>Translated_204_residues
MPRTVEVPEYTIAPEVEIDPRTTALIVVDMQNDFVRPEGKLFVPDAPATVPKIQALLAFARQHGIFTVFTQDTHYPGDPE
FPIWGEHCVAGTWGWQIIDELAPREGELVLQKRRYDAFYGTPLDHELRLRKIEQLIICGTVASICVHYTAASAALRWYGV
IIPVDATSALHPFDLEAANRQTAFLFRGVLTRADGVRLRSGVPA
>Mature_203_residues
PRTVEVPEYTIAPEVEIDPRTTALIVVDMQNDFVRPEGKLFVPDAPATVPKIQALLAFARQHGIFTVFTQDTHYPGDPEF
PIWGEHCVAGTWGWQIIDELAPREGELVLQKRRYDAFYGTPLDHELRLRKIEQLIICGTVASICVHYTAASAALRWYGVI
IPVDATSALHPFDLEAANRQTAFLFRGVLTRADGVRLRSGVPA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=190, Percent_Identity=35.2631578947368, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786811, Length=160, Percent_Identity=28.75, Blast_Score=65, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321401, Length=187, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 22793; Mature: 22662

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTVEVPEYTIAPEVEIDPRTTALIVVDMQNDFVRPEGKLFVPDAPATVPKIQALLAFA
CCCCCCCCCEEECCCEEECCCCEEEEEEECCCCEECCCCCEECCCCCCHHHHHHHHHHHH
RQHGIFTVFTQDTHYPGDPEFPIWGEHCVAGTWGWQIIDELAPREGELVLQKRRYDAFYG
HHCCEEEEEECCCCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCC
TPLDHELRLRKIEQLIICGTVASICVHYTAASAALRWYGVIIPVDATSALHPFDLEAANR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCC
QTAFLFRGVLTRADGVRLRSGVPA
HHHHHHHHHHHCCCCEEECCCCCC
>Mature Secondary Structure 
PRTVEVPEYTIAPEVEIDPRTTALIVVDMQNDFVRPEGKLFVPDAPATVPKIQALLAFA
CCCCCCCCEEECCCEEECCCCEEEEEEECCCCEECCCCCEECCCCCCHHHHHHHHHHHH
RQHGIFTVFTQDTHYPGDPEFPIWGEHCVAGTWGWQIIDELAPREGELVLQKRRYDAFYG
HHCCEEEEEECCCCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCC
TPLDHELRLRKIEQLIICGTVASICVHYTAASAALRWYGVIIPVDATSALHPFDLEAANR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCC
QTAFLFRGVLTRADGVRLRSGVPA
HHHHHHHHHHHCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA