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The map label for this gene is thrA [C]

Identifier: 221635820

GI number: 221635820

Start: 400731

End: 402161

Strand: Reverse

Name: thrA [C]

Synonym: trd_A0414

Alternate gene names: 221635820

Gene position: 402161-400731 (Counterclockwise)

Preceding gene: 221635825

Following gene: 221635818

Centisome position: 43.82

GC content: 66.74

Gene sequence:

>1431_bases
ATGCGGGTGATGAAGTTCGGGGGAACGTCGGTCGGCAATGCCGTCGCGATCGATCGCACGGCACGGATCATCGCCGACGC
CTATCGCGGTGACGGCCGCCTCGTTGCCGTCGTCTCGGCGATGAGCGGCGTCACCAACCAGCTCATCGCGGCCGCTCAAG
CTGCAGCCGAGGGAGACGAGCAGGCAGCAGCGAGGCTCCAACAGGAACTCGTCGAGCGACATCGCGGGGCGGCCGCGGAA
CTGATCCCGACCGCAGCGCGCCTGGAGGCGACGGTGAGCCGCCTGACCGAACTCGCCACCCGTTGTGCGCGCCTCGTCGA
GTCGGTCCACGTACTCCGCGACCTCTCGCCGCGTGCTCAGGACTGGATCGTCTCCTTCGGCGAGCGGATGAGTGCAGTCC
TCATAGCAGCTGTCCTCGAGGAACGCGGTATCCCAGCCGTGCCGGTCGACAGCGACAAGGTCATCGTGACCGATGACCAC
TTCGGGAGCGCCAACCCGTTGCTCGACGAAACCCGGGCGCGCGCCGAGGCGATCCTCGTCCCGCTTCTCGAAGCTGGCAA
GCTGCCCGTCGTCACCGGCTTCTTCGGTGCCACGCCGAAGGGTGCGGTCACCACGCTCGGCCGCGGCGGGTCCGACTTCT
CCGCCTCGATCCTCGCTCACGCGCTCGACGCCGACGAGGTGTGGATCTGGACCGACGTCGATGGCGTCATGACGGCTGAT
CCGCGACTCGTCCCCAGTGCGCGCACGCTTCCCGCGATCTCCTTCGCCGAGGCGACCGAACTCGCCTACTTCGGCGCCAA
GGTGATCCACCCGCGCACCATGCAACCGGCTGCCGAGCGTGGCATTCCGATCTGGATCAAGAACACCTTCAACCCCGAGC
ACCCGGGCACACGTATCGGCCCGGACACCAGCTCGAACGGTTCGGTGGTCAAGGCGATCACCGCGATTCCCGGCGTGAGC
GTCATCACCGTCGAGGGATCCGGCTTCCTCAGCGTCGCCGACGTCACTGCCCGCGTCTTCGAGACCGTCGGCCGGACTGG
CGTCAACGTCTTTATGGTCTTCCAGGCGTCGTCCCAGCACAGCCTGGGTTTTGTCGTCCGGCGCACCGATGCCAGCAAAG
TGTTGCGTGCGCTGGAGCGGGAGTTCGAACTCGATCTCCTCAAGGGACGCGTCGCACGCCTCTGGGAAGAACCCGGTATG
GCCATCGTCGCCGTCGTCGGTGCCGGGATGCGCGGGACACCCGGTGTCGCCGGCCGCGTCTTCGGGACCCTCGGCCAACA
TCGCATCAACATCGTCGCCATCGCGCAAGGGTCGTCGGAACTCAACATTTCATTCGTGATCCGCGAAGACGAGGTCGCAC
GCGCCGTTCCAGCGATTCACGACGCGTTCGGGCTCGGGGGATCTGCTGGCGAACGCTCGGCTGGTTCCTGA

Upstream 100 bases:

>100_bases
TCGTCCCGCATGCTGTGAGACGCGTTCGAGTATCCTTTCCCCTTCGAGAAGCTTTGGAGCACGTATGGAGCCGAAGGCTC
CCAGAAAGGATTCCAACGCG

Downstream 100 bases:

>100_bases
TGCCGGCACGCACGACGGGAGCGCTCGCTGAGCAACGCCCAGAGCGATATCGCCAGGATCACGAGCGGTAACACCACGAA
TCCGATCAGAAAGATGAGCG

Product: asparate kinase, monofunctional class

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MRVMKFGGTSVGNAVAIDRTARIIADAYRGDGRLVAVVSAMSGVTNQLIAAAQAAAEGDEQAAARLQQELVERHRGAAAE
LIPTAARLEATVSRLTELATRCARLVESVHVLRDLSPRAQDWIVSFGERMSAVLIAAVLEERGIPAVPVDSDKVIVTDDH
FGSANPLLDETRARAEAILVPLLEAGKLPVVTGFFGATPKGAVTTLGRGGSDFSASILAHALDADEVWIWTDVDGVMTAD
PRLVPSARTLPAISFAEATELAYFGAKVIHPRTMQPAAERGIPIWIKNTFNPEHPGTRIGPDTSSNGSVVKAITAIPGVS
VITVEGSGFLSVADVTARVFETVGRTGVNVFMVFQASSQHSLGFVVRRTDASKVLRALEREFELDLLKGRVARLWEEPGM
AIVAVVGAGMRGTPGVAGRVFGTLGQHRINIVAIAQGSSELNISFVIREDEVARAVPAIHDAFGLGGSAGERSAGS

Sequences:

>Translated_476_residues
MRVMKFGGTSVGNAVAIDRTARIIADAYRGDGRLVAVVSAMSGVTNQLIAAAQAAAEGDEQAAARLQQELVERHRGAAAE
LIPTAARLEATVSRLTELATRCARLVESVHVLRDLSPRAQDWIVSFGERMSAVLIAAVLEERGIPAVPVDSDKVIVTDDH
FGSANPLLDETRARAEAILVPLLEAGKLPVVTGFFGATPKGAVTTLGRGGSDFSASILAHALDADEVWIWTDVDGVMTAD
PRLVPSARTLPAISFAEATELAYFGAKVIHPRTMQPAAERGIPIWIKNTFNPEHPGTRIGPDTSSNGSVVKAITAIPGVS
VITVEGSGFLSVADVTARVFETVGRTGVNVFMVFQASSQHSLGFVVRRTDASKVLRALEREFELDLLKGRVARLWEEPGM
AIVAVVGAGMRGTPGVAGRVFGTLGQHRINIVAIAQGSSELNISFVIREDEVARAVPAIHDAFGLGGSAGERSAGS
>Mature_476_residues
MRVMKFGGTSVGNAVAIDRTARIIADAYRGDGRLVAVVSAMSGVTNQLIAAAQAAAEGDEQAAARLQQELVERHRGAAAE
LIPTAARLEATVSRLTELATRCARLVESVHVLRDLSPRAQDWIVSFGERMSAVLIAAVLEERGIPAVPVDSDKVIVTDDH
FGSANPLLDETRARAEAILVPLLEAGKLPVVTGFFGATPKGAVTTLGRGGSDFSASILAHALDADEVWIWTDVDGVMTAD
PRLVPSARTLPAISFAEATELAYFGAKVIHPRTMQPAAERGIPIWIKNTFNPEHPGTRIGPDTSSNGSVVKAITAIPGVS
VITVEGSGFLSVADVTARVFETVGRTGVNVFMVFQASSQHSLGFVVRRTDASKVLRALEREFELDLLKGRVARLWEEPGM
AIVAVVGAGMRGTPGVAGRVFGTLGQHRINIVAIAQGSSELNISFVIREDEVARAVPAIHDAFGLGGSAGERSAGS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=465, Percent_Identity=37.6344086021505, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1790455, Length=468, Percent_Identity=34.6153846153846, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1790376, Length=429, Percent_Identity=29.1375291375291, Blast_Score=140, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320893, Length=507, Percent_Identity=30.7692307692308, Blast_Score=201, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 50128; Mature: 50128

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVMKFGGTSVGNAVAIDRTARIIADAYRGDGRLVAVVSAMSGVTNQLIAAAQAAAEGDE
CEEEEECCCCCCCEEEECHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHH
QAAARLQQELVERHRGAAAELIPTAARLEATVSRLTELATRCARLVESVHVLRDLSPRAQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DWIVSFGERMSAVLIAAVLEERGIPAVPVDSDKVIVTDDHFGSANPLLDETRARAEAILV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
PLLEAGKLPVVTGFFGATPKGAVTTLGRGGSDFSASILAHALDADEVWIWTDVDGVMTAD
HHHCCCCCCEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCEEECC
PRLVPSARTLPAISFAEATELAYFGAKVIHPRTMQPAAERGIPIWIKNTFNPEHPGTRIG
CCCCCCCCCCCCEEHHHHHHHHHHCHHEECCCCCCHHHHCCCEEEEECCCCCCCCCCCCC
PDTSSNGSVVKAITAIPGVSVITVEGSGFLSVADVTARVFETVGRTGVNVFMVFQASSQH
CCCCCCCCEEEEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCCCC
SLGFVVRRTDASKVLRALEREFELDLLKGRVARLWEEPGMAIVAVVGAGMRGTPGVAGRV
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
FGTLGQHRINIVAIAQGSSELNISFVIREDEVARAVPAIHDAFGLGGSAGERSAGS
HHHCCCCEEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRVMKFGGTSVGNAVAIDRTARIIADAYRGDGRLVAVVSAMSGVTNQLIAAAQAAAEGDE
CEEEEECCCCCCCEEEECHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHH
QAAARLQQELVERHRGAAAELIPTAARLEATVSRLTELATRCARLVESVHVLRDLSPRAQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DWIVSFGERMSAVLIAAVLEERGIPAVPVDSDKVIVTDDHFGSANPLLDETRARAEAILV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
PLLEAGKLPVVTGFFGATPKGAVTTLGRGGSDFSASILAHALDADEVWIWTDVDGVMTAD
HHHCCCCCCEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCEEECC
PRLVPSARTLPAISFAEATELAYFGAKVIHPRTMQPAAERGIPIWIKNTFNPEHPGTRIG
CCCCCCCCCCCCEEHHHHHHHHHHCHHEECCCCCCHHHHCCCEEEEECCCCCCCCCCCCC
PDTSSNGSVVKAITAIPGVSVITVEGSGFLSVADVTARVFETVGRTGVNVFMVFQASSQH
CCCCCCCCEEEEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCCCC
SLGFVVRRTDASKVLRALEREFELDLLKGRVARLWEEPGMAIVAVVGAGMRGTPGVAGRV
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
FGTLGQHRINIVAIAQGSSELNISFVIREDEVARAVPAIHDAFGLGGSAGERSAGS
HHHCCCCEEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]