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The map label for this gene is mrpA [H]

Identifier: 221635781

GI number: 221635781

Start: 360144

End: 362474

Strand: Reverse

Name: mrpA [H]

Synonym: trd_A0375

Alternate gene names: 221635781

Gene position: 362474-360144 (Counterclockwise)

Preceding gene: 221635783

Following gene: 221635780

Centisome position: 39.5

GC content: 65.38

Gene sequence:

>2331_bases
GTGCTCGCTGGTCTCGCGTCGTTGTTCATTCTGGCGTTCCTCGCCCCCTGGCTCGGGGATCGGCTCGCTGCCCGCTTCGG
GTATTTGGCGGCACTCACCTCGTTCGTGCTCTTCGGCTGGTACGCGAGCCAGCTCCCGGCGGTGACACACGGACCGACAC
GCGTCGAAACGATTTCGTGGCTCCCCACGCTCGGGGCAGATCTCACGCTCCGCTTGGATGGGCTGGGACTCCTGTTCGCG
CTCCTCGTCACTGGCATCGGTGCACTCATCGCGCTCTATTCGGTCGCGTACCTCGAACATCATCCGCGCCGCCGGGCCTT
TTGGGTCGCATTCCTCGCCTTCGAGGCAGCGATGCTCGGCTTGGTGCTCGCCGATGACGTGATTCTCCTGTTCGTCTTCT
GGGAACTCACCAGCATCACTTCGTTCTTCCTCATCGGTTTGGAAAACGAACGGGAGCGAGCACGGACGAGTGCGACGCAG
GCCTTGATCGTCACCGGAAGCGGTGGGCTCGCCCTCTTGACCGGCCTCCTCATCCTGGTGCAGCGCACCGGCAGCCTGCG
ACTGAGCGAAATCCTGGCCCAGGGGCCTCTTGATGCCGACGGGCTGGTCACGACCGCTTTCCTCCTGATTCTGCTCGGTG
CTGCAGCCAAGTCGGCTCAGGTCCCGTTGCACTTCTGGCTTCCCAACGCCATGGAGGCTCCCACTCCAGTGAGCGCTTAT
CTCCATTCGGCAACCATGGTCAAGGCCGGCGTCTACTTGCTGGCGCGAATGACTCCCGCGTTCGGCGAGCACCCCTGGTG
GCAACCGAGCCTCGGCGTGCTCGGCGGCGCGACTGCCCTGGTCGGCGCGCTGCTCGCGGTCCAGCAGCGCGATCTCAAGC
GGCTGCTCGCCTACAGCACGATCAGCGCGCTCGGGACGCTCGTCTTCCTGCTCAGCTTCGGCGAGCCCGGCTGGCTCCCC
TTCGCCCTCTTCTTGCTCGCCCATGCGCTGTACAAGGGAGGAGCGTTCCTGCTCGCTGGCGCCATCGACCATGCGACCGG
CGAGCGGGTGCTCGATCGTCTCGGCGGACTGGCTGGACTTTTTCCCGGACTCGCCATCGTCGCCGGACTCGTCGTCCTGG
CTGCCGCTGGCCTTCCGCCGACGCTTTCTTTCGTCGCCAAGGAACTCGTTCTCGAAACGTCCTGGAACGAGCTGCCACTC
CCAGGCCTGCTCGTTCTGCTCGCCGCGCTCAGTGGACAGACAGCAGTCGCTTGGCTCCTTTTGCGACCCTTTGTCGGCCA
GCCGAGGACGGCTCCCGAGCACGCCCACGCTCCAGCCTGGCAGCTCCTGACCGCACCCGCGCTGCTGGGTGCCGCCGCGT
CCCTTTCCGGTCTTCTCACTAGTTCCCTCGGCCCACTGGCGAGTGCGGTCGTCGCCAGCCTGTCGGGCCATGAGCCAACC
AGCGTCAAGCTCGCCCTGTGGCACGGCCTCACCCCTGCACTCGGCCTGAGCCTGCTCGTCCTGCTGCTCGGCGGAGCGCT
CGCCGTCAGCTGGCCGATCGCGCTCCGACTCGGCGATCGCCTCCGCACGACTGAATCGTGGAGCCCAACAGCCGGAGCTC
TGGCGGCGTGGGGGAGCGAGAGCTGGTACTTCCGAACACTGGCGGTCATCAACAGCGTGGCTATCCGGCAGACCCGACTG
CTGCAGAGCGGCTACTTGCGCGTCTACGTCATGGTCACAGTGTTGACGCTCGTCTCACTCTCGCTGCTCACCGTGGGCCG
ATACCGTGCCGAACTCCTCGCCCACTGGTCTCCTCCACACCTGTCAGCGATCGATGTCGTCGACGCGAGCTTAGCGCTCA
TCATCCTCGTGGCCGCGCTCGTGGCAGTCCGCGCGACCGAGCGACTCGCTGCCATTGCGGCGCTCGGTGCTCTCGGTTTC
AGCCTCGCCGTGCTCTATGCGCGCTTCAGCGCACCGGATCTGGCGATCACGCAAGTCCTCGTCGATACCTTGACCGTCGT
GCTCCTCGTCTTCGCCTTCTATCGCTTGCCGCGTTATGCCCGCCTCTCGACGACCCGCGCCCGTGTCCGCGATGCGATCG
TCGCCATGCTTTTTGGGATGACGATGAGCCTTTTCGCGTTCCTGATGATCGGTTCCCCGGCTCGCGAGCGCATTTCGACC
TTCTTCCTCGAGCAGAGCTACCCGGCGGCACACGGGCGGAACGTGGTCAATGTCATCCTGGTCGATTTCCGTGCCCTGGA
CACGCTCGGTGAGATCACCGTGCTGGGCTTGGCCGGTCTCGGAGTGACAGCCTTGCTTTACCGGCGTCGGCAGAAGGAGG
AAGGAACGTGA

Upstream 100 bases:

>100_bases
CGGTCGCCCCGCGAGGAGGCTCGGGCACCACAGGCAGGGAGCAGCGAGCCTCTCGAACGCACCATCGCACGCCGACGGAA
CGAGCGTCAGAGGAGGGTTC

Downstream 100 bases:

>100_bases
GTACCCTTCCCTCGATCATCTTGCGAACAGCCGTCCGCTGGATCTCACCGCTTCTCTTGCTTTTCAGCCTCTTCCTGCTG
TTGCGCGGACATCACGAGCC

Product: Na(+)/H(+) antiporter subunit A (Multiple resistance and pH homeostasis protein A) (Mrp complex subunit A)

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 776; Mature: 776

Protein sequence:

>776_residues
MLAGLASLFILAFLAPWLGDRLAARFGYLAALTSFVLFGWYASQLPAVTHGPTRVETISWLPTLGADLTLRLDGLGLLFA
LLVTGIGALIALYSVAYLEHHPRRRAFWVAFLAFEAAMLGLVLADDVILLFVFWELTSITSFFLIGLENERERARTSATQ
ALIVTGSGGLALLTGLLILVQRTGSLRLSEILAQGPLDADGLVTTAFLLILLGAAAKSAQVPLHFWLPNAMEAPTPVSAY
LHSATMVKAGVYLLARMTPAFGEHPWWQPSLGVLGGATALVGALLAVQQRDLKRLLAYSTISALGTLVFLLSFGEPGWLP
FALFLLAHALYKGGAFLLAGAIDHATGERVLDRLGGLAGLFPGLAIVAGLVVLAAAGLPPTLSFVAKELVLETSWNELPL
PGLLVLLAALSGQTAVAWLLLRPFVGQPRTAPEHAHAPAWQLLTAPALLGAAASLSGLLTSSLGPLASAVVASLSGHEPT
SVKLALWHGLTPALGLSLLVLLLGGALAVSWPIALRLGDRLRTTESWSPTAGALAAWGSESWYFRTLAVINSVAIRQTRL
LQSGYLRVYVMVTVLTLVSLSLLTVGRYRAELLAHWSPPHLSAIDVVDASLALIILVAALVAVRATERLAAIAALGALGF
SLAVLYARFSAPDLAITQVLVDTLTVVLLVFAFYRLPRYARLSTTRARVRDAIVAMLFGMTMSLFAFLMIGSPARERIST
FFLEQSYPAAHGRNVVNVILVDFRALDTLGEITVLGLAGLGVTALLYRRRQKEEGT

Sequences:

>Translated_776_residues
MLAGLASLFILAFLAPWLGDRLAARFGYLAALTSFVLFGWYASQLPAVTHGPTRVETISWLPTLGADLTLRLDGLGLLFA
LLVTGIGALIALYSVAYLEHHPRRRAFWVAFLAFEAAMLGLVLADDVILLFVFWELTSITSFFLIGLENERERARTSATQ
ALIVTGSGGLALLTGLLILVQRTGSLRLSEILAQGPLDADGLVTTAFLLILLGAAAKSAQVPLHFWLPNAMEAPTPVSAY
LHSATMVKAGVYLLARMTPAFGEHPWWQPSLGVLGGATALVGALLAVQQRDLKRLLAYSTISALGTLVFLLSFGEPGWLP
FALFLLAHALYKGGAFLLAGAIDHATGERVLDRLGGLAGLFPGLAIVAGLVVLAAAGLPPTLSFVAKELVLETSWNELPL
PGLLVLLAALSGQTAVAWLLLRPFVGQPRTAPEHAHAPAWQLLTAPALLGAAASLSGLLTSSLGPLASAVVASLSGHEPT
SVKLALWHGLTPALGLSLLVLLLGGALAVSWPIALRLGDRLRTTESWSPTAGALAAWGSESWYFRTLAVINSVAIRQTRL
LQSGYLRVYVMVTVLTLVSLSLLTVGRYRAELLAHWSPPHLSAIDVVDASLALIILVAALVAVRATERLAAIAALGALGF
SLAVLYARFSAPDLAITQVLVDTLTVVLLVFAFYRLPRYARLSTTRARVRDAIVAMLFGMTMSLFAFLMIGSPARERIST
FFLEQSYPAAHGRNVVNVILVDFRALDTLGEITVLGLAGLGVTALLYRRRQKEEGT
>Mature_776_residues
MLAGLASLFILAFLAPWLGDRLAARFGYLAALTSFVLFGWYASQLPAVTHGPTRVETISWLPTLGADLTLRLDGLGLLFA
LLVTGIGALIALYSVAYLEHHPRRRAFWVAFLAFEAAMLGLVLADDVILLFVFWELTSITSFFLIGLENERERARTSATQ
ALIVTGSGGLALLTGLLILVQRTGSLRLSEILAQGPLDADGLVTTAFLLILLGAAAKSAQVPLHFWLPNAMEAPTPVSAY
LHSATMVKAGVYLLARMTPAFGEHPWWQPSLGVLGGATALVGALLAVQQRDLKRLLAYSTISALGTLVFLLSFGEPGWLP
FALFLLAHALYKGGAFLLAGAIDHATGERVLDRLGGLAGLFPGLAIVAGLVVLAAAGLPPTLSFVAKELVLETSWNELPL
PGLLVLLAALSGQTAVAWLLLRPFVGQPRTAPEHAHAPAWQLLTAPALLGAAASLSGLLTSSLGPLASAVVASLSGHEPT
SVKLALWHGLTPALGLSLLVLLLGGALAVSWPIALRLGDRLRTTESWSPTAGALAAWGSESWYFRTLAVINSVAIRQTRL
LQSGYLRVYVMVTVLTLVSLSLLTVGRYRAELLAHWSPPHLSAIDVVDASLALIILVAALVAVRATERLAAIAALGALGF
SLAVLYARFSAPDLAITQVLVDTLTVVLLVFAFYRLPRYARLSTTRARVRDAIVAMLFGMTMSLFAFLMIGSPARERIST
FFLEQSYPAAHGRNVVNVILVDFRALDTLGEITVLGLAGLGVTALLYRRRQKEEGT

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=329, Percent_Identity=34.6504559270517, Blast_Score=157, Evalue=5e-38,
Organism=Homo sapiens, GI251831116, Length=321, Percent_Identity=28.9719626168224, Blast_Score=67, Evalue=9e-11,
Organism=Escherichia coli, GI1788614, Length=542, Percent_Identity=31.3653136531365, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788829, Length=395, Percent_Identity=36.2025316455696, Blast_Score=163, Evalue=4e-41,
Organism=Escherichia coli, GI1788827, Length=515, Percent_Identity=28.1553398058252, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788831, Length=343, Percent_Identity=30.9037900874636, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1788613, Length=354, Percent_Identity=29.9435028248588, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI145693160, Length=345, Percent_Identity=30.1449275362319, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI2367154, Length=373, Percent_Identity=29.7587131367292, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 82566; Mature: 82566

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00318 HMG_COA_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAGLASLFILAFLAPWLGDRLAARFGYLAALTSFVLFGWYASQLPAVTHGPTRVETISW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LPTLGADLTLRLDGLGLLFALLVTGIGALIALYSVAYLEHHPRRRAFWVAFLAFEAAMLG
HHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LVLADDVILLFVFWELTSITSFFLIGLENERERARTSATQALIVTGSGGLALLTGLLILV
HHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCEEEEEECCCCHHHHHHHHHHH
QRTGSLRLSEILAQGPLDADGLVTTAFLLILLGAAAKSAQVPLHFWLPNAMEAPTPVSAY
HCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHH
LHSATMVKAGVYLLARMTPAFGEHPWWQPSLGVLGGATALVGALLAVQQRDLKRLLAYST
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISALGTLVFLLSFGEPGWLPFALFLLAHALYKGGAFLLAGAIDHATGERVLDRLGGLAGL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCHHHH
FPGLAIVAGLVVLAAAGLPPTLSFVAKELVLETSWNELPLPGLLVLLAALSGQTAVAWLL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHH
LRPFVGQPRTAPEHAHAPAWQLLTAPALLGAAASLSGLLTSSLGPLASAVVASLSGHEPT
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SVKLALWHGLTPALGLSLLVLLLGGALAVSWPIALRLGDRLRTTESWSPTAGALAAWGSE
EEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHCCCC
SWYFRTLAVINSVAIRQTRLLQSGYLRVYVMVTVLTLVSLSLLTVGRYRAELLAHWSPPH
CHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LSAIDVVDASLALIILVAALVAVRATERLAAIAALGALGFSLAVLYARFSAPDLAITQVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VDTLTVVLLVFAFYRLPRYARLSTTRARVRDAIVAMLFGMTMSLFAFLMIGSPARERIST
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FFLEQSYPAAHGRNVVNVILVDFRALDTLGEITVLGLAGLGVTALLYRRRQKEEGT
HHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLAGLASLFILAFLAPWLGDRLAARFGYLAALTSFVLFGWYASQLPAVTHGPTRVETISW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LPTLGADLTLRLDGLGLLFALLVTGIGALIALYSVAYLEHHPRRRAFWVAFLAFEAAMLG
HHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LVLADDVILLFVFWELTSITSFFLIGLENERERARTSATQALIVTGSGGLALLTGLLILV
HHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCEEEEEECCCCHHHHHHHHHHH
QRTGSLRLSEILAQGPLDADGLVTTAFLLILLGAAAKSAQVPLHFWLPNAMEAPTPVSAY
HCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHH
LHSATMVKAGVYLLARMTPAFGEHPWWQPSLGVLGGATALVGALLAVQQRDLKRLLAYST
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISALGTLVFLLSFGEPGWLPFALFLLAHALYKGGAFLLAGAIDHATGERVLDRLGGLAGL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCHHHH
FPGLAIVAGLVVLAAAGLPPTLSFVAKELVLETSWNELPLPGLLVLLAALSGQTAVAWLL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHH
LRPFVGQPRTAPEHAHAPAWQLLTAPALLGAAASLSGLLTSSLGPLASAVVASLSGHEPT
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SVKLALWHGLTPALGLSLLVLLLGGALAVSWPIALRLGDRLRTTESWSPTAGALAAWGSE
EEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHCCCC
SWYFRTLAVINSVAIRQTRLLQSGYLRVYVMVTVLTLVSLSLLTVGRYRAELLAHWSPPH
CHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LSAIDVVDASLALIILVAALVAVRATERLAAIAALGALGFSLAVLYARFSAPDLAITQVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VDTLTVVLLVFAFYRLPRYARLSTTRARVRDAIVAMLFGMTMSLFAFLMIGSPARERIST
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FFLEQSYPAAHGRNVVNVILVDFRALDTLGEITVLGLAGLGVTALLYRRRQKEEGT
HHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]