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The map label for this gene is xsc [H]

Identifier: 221635734

GI number: 221635734

Start: 314715

End: 316361

Strand: Reverse

Name: xsc [H]

Synonym: trd_A0328

Alternate gene names: 221635734

Gene position: 316361-314715 (Counterclockwise)

Preceding gene: 221635735

Following gene: 221635733

Centisome position: 34.47

GC content: 59.56

Gene sequence:

>1647_bases
GTGGCTAAGGTCTACGAGCTCCTTTCGCGCCAGCTCATCGGCCTCGGTTCCGATACGATCTTTGGCCTCGTAGGGAGTGG
CAACTTCTTCCTGGCCGAGAGTCTCGTCACCCATGGTGCGAAGTTCGTGGCTGCGCGACATGAGGCCGGAGCCTTAGCGA
TGGCCGATGCCTGGGCTCGTCTGACACAAAAGGTCGGAATCTGCACTGTTCACATGGGTCCAGGTTTCACGAACATCATT
ACACCACTCGTCGAAGCTGCAAAAGCACGGACACCGCTCCTCGTCCTGACCGCCGATGTGTCGACAGGGGCCGTTACGTC
CACGTTCGCCATCGATATCGATGCCTTGGCCACCGCCAGTGGAGCCATCCCTGAACGGTTGTGGAAGAACTCCACAGCAA
CCCAAGACCTTGTCCGTGCCTATCGCCGCGCTCGATACGAGCGTCGGCCAGTCGTCTTCGTCATGCCACAAGACATCCAA
GCAGCGGAGTCCACGGCAGCTGTCATAGCTCCAGAGGACAATGCCTACTTTTCTGTGATCCCTGACCGAAGCATACTCGA
GAAGATCGTCTCCCTCGTTCGGGACTCGCGTCACCCTCTCATTTTGGCAGGCCGCGGCTCAGTTCTCGCGGGAGCGAGGG
CAGGTCTCGAGAAACTCGCCGAAGCCATTGGAGCTCTTCTCGCCACGACAGCGAATGCCCACGGACTGTTCGCTGGGAAC
CCCTGGTCGATCGGTATCGCTGGCGGATTCTCGTCTCCCGGAGCAGTCGAACTCTTCCGACAGGCCGATCTGATTTTGGC
CTTCGGCGCCTCCCTCACCGTTTGGACCCGCCGCCACGGTCGTCTCTTTCCAGAGTCGTGCCCGATCGTGCAGATCGACC
TCGACCCCGCCGCGCTGCACCGCCAATACCCGGTCACGCTCGCTTGCGTCGGCGACGCCCGTGCCACGGCAGAAGCACTA
CTCGAGCTCTTGCCCGACAAGCCAGTAGGGCAATGGCGAACACCGTCGACGCTCCAGTTCATTCAGGAACACTCCTGGAT
TAACACACCGTTCGAGGATGCCAGCACGGAGAACACGATCGACCCGAGAACGCTTTCACTGTTCCTGGAACAGAACCTTC
CACCGGATCGTATCCTCGTCCTCGACTCTGGTCACTTCATGGCATACCCGGTGATGTATCTGAGTGTCCCAGATGAAAAA
GGTTTTGTTTTCACGCAAGCATTACAGTCGATCGGCTTCGGTTTGCCGAATGCGATCGGAGCAGCCATCGCCTATCCAGA
TCGTCTCACCATCGCAGCGACTGGTGACGGAGGACTGCTGCTCTCGTTGAGCGAACTCGAGACAGTCGCACGCTATCGGC
TCCCCATCCTCATCGTGGTTTACAACGATGCCGCCTATGGGGCCGAGGTTCATCACTTCGCTCCGATGGGTTGGCCAACT
TCCACCGTGCAATTCCCTGACACGAACATCGCCGAGCTCGCTCGCGCCCTGGGTATGCACGCCTTTACCGTCCGCACACA
GCGCGACCTGGAATCGCTCCGGGAGGCGCTCTCCGATCGCTCCTGCTATCCCATGCTGCTGGATGCCAAGGTCAACCCAG
CGGTACGCGGCGGAGAATGGTTCGATCTGGCTTTCGAGGGTCACTAA

Upstream 100 bases:

>100_bases
AAATCTTCGCCGAGCAGGTTCGACTCCAACAGGGTCTGACGACGCCAGCCCAAGCTGTCACGATATAGGTTTTGGTCAGT
TCAAGAGAAAGGGGTTCACC

Downstream 100 bases:

>100_bases
CTCGGTGTTCTGTTTGGAAAGGAGGACACATGGCCCTGTGGTTGACCCATGTCGGCGTTCGCGTGCCGAATCTGCAGGAC
TCCATCGAGTTTCACACGTC

Product: thiamine pyrophosphate enzyme, central domain family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MAKVYELLSRQLIGLGSDTIFGLVGSGNFFLAESLVTHGAKFVAARHEAGALAMADAWARLTQKVGICTVHMGPGFTNII
TPLVEAAKARTPLLVLTADVSTGAVTSTFAIDIDALATASGAIPERLWKNSTATQDLVRAYRRARYERRPVVFVMPQDIQ
AAESTAAVIAPEDNAYFSVIPDRSILEKIVSLVRDSRHPLILAGRGSVLAGARAGLEKLAEAIGALLATTANAHGLFAGN
PWSIGIAGGFSSPGAVELFRQADLILAFGASLTVWTRRHGRLFPESCPIVQIDLDPAALHRQYPVTLACVGDARATAEAL
LELLPDKPVGQWRTPSTLQFIQEHSWINTPFEDASTENTIDPRTLSLFLEQNLPPDRILVLDSGHFMAYPVMYLSVPDEK
GFVFTQALQSIGFGLPNAIGAAIAYPDRLTIAATGDGGLLLSLSELETVARYRLPILIVVYNDAAYGAEVHHFAPMGWPT
STVQFPDTNIAELARALGMHAFTVRTQRDLESLREALSDRSCYPMLLDAKVNPAVRGGEWFDLAFEGH

Sequences:

>Translated_548_residues
MAKVYELLSRQLIGLGSDTIFGLVGSGNFFLAESLVTHGAKFVAARHEAGALAMADAWARLTQKVGICTVHMGPGFTNII
TPLVEAAKARTPLLVLTADVSTGAVTSTFAIDIDALATASGAIPERLWKNSTATQDLVRAYRRARYERRPVVFVMPQDIQ
AAESTAAVIAPEDNAYFSVIPDRSILEKIVSLVRDSRHPLILAGRGSVLAGARAGLEKLAEAIGALLATTANAHGLFAGN
PWSIGIAGGFSSPGAVELFRQADLILAFGASLTVWTRRHGRLFPESCPIVQIDLDPAALHRQYPVTLACVGDARATAEAL
LELLPDKPVGQWRTPSTLQFIQEHSWINTPFEDASTENTIDPRTLSLFLEQNLPPDRILVLDSGHFMAYPVMYLSVPDEK
GFVFTQALQSIGFGLPNAIGAAIAYPDRLTIAATGDGGLLLSLSELETVARYRLPILIVVYNDAAYGAEVHHFAPMGWPT
STVQFPDTNIAELARALGMHAFTVRTQRDLESLREALSDRSCYPMLLDAKVNPAVRGGEWFDLAFEGH
>Mature_547_residues
AKVYELLSRQLIGLGSDTIFGLVGSGNFFLAESLVTHGAKFVAARHEAGALAMADAWARLTQKVGICTVHMGPGFTNIIT
PLVEAAKARTPLLVLTADVSTGAVTSTFAIDIDALATASGAIPERLWKNSTATQDLVRAYRRARYERRPVVFVMPQDIQA
AESTAAVIAPEDNAYFSVIPDRSILEKIVSLVRDSRHPLILAGRGSVLAGARAGLEKLAEAIGALLATTANAHGLFAGNP
WSIGIAGGFSSPGAVELFRQADLILAFGASLTVWTRRHGRLFPESCPIVQIDLDPAALHRQYPVTLACVGDARATAEALL
ELLPDKPVGQWRTPSTLQFIQEHSWINTPFEDASTENTIDPRTLSLFLEQNLPPDRILVLDSGHFMAYPVMYLSVPDEKG
FVFTQALQSIGFGLPNAIGAAIAYPDRLTIAATGDGGLLLSLSELETVARYRLPILIVVYNDAAYGAEVHHFAPMGWPTS
TVQFPDTNIAELARALGMHAFTVRTQRDLESLREALSDRSCYPMLLDAKVNPAVRGGEWFDLAFEGH

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=25.3097345132743, Blast_Score=113, Evalue=5e-25,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=26.140350877193, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI1787096, Length=550, Percent_Identity=25.2727272727273, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1790104, Length=512, Percent_Identity=23.828125, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786717, Length=468, Percent_Identity=24.5726495726496, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788716, Length=541, Percent_Identity=24.3992606284658, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17542570, Length=569, Percent_Identity=24.6045694200351, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=24.006908462867, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=21.7770034843206, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017820
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.3.3.15 [H]

Molecular weight: Translated: 59107; Mature: 58976

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVYELLSRQLIGLGSDTIFGLVGSGNFFLAESLVTHGAKFVAARHEAGALAMADAWAR
CCHHHHHHHHHHHCCCCCEEEEEEECCCEEEHHHHHHCCHHEEEECCCCCCCHHHHHHHH
LTQKVGICTVHMGPGFTNIITPLVEAAKARTPLLVLTADVSTGAVTSTFAIDIDALATAS
HHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEHHHHHCCC
GAIPERLWKNSTATQDLVRAYRRARYERRPVVFVMPQDIQAAESTAAVIAPEDNAYFSVI
CCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCCEEEEC
PDRSILEKIVSLVRDSRHPLILAGRGSVLAGARAGLEKLAEAIGALLATTANAHGLFAGN
CCHHHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
PWSIGIAGGFSSPGAVELFRQADLILAFGASLTVWTRRHGRLFPESCPIVQIDLDPAALH
CEEEEEECCCCCCCHHHHHHHCCEEEEECCEEEEEECCCCCCCCCCCCEEEEECCHHHHC
RQYPVTLACVGDARATAEALLELLPDKPVGQWRTPSTLQFIQEHSWINTPFEDASTENTI
CCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
DPRTLSLFLEQNLPPDRILVLDSGHFMAYPVMYLSVPDEKGFVFTQALQSIGFGLPNAIG
CCHHEEEEECCCCCCCEEEEEECCCEEEEEEEEEECCCCCCCHHHHHHHHHCCCCCHHHH
AAIAYPDRLTIAATGDGGLLLSLSELETVARYRLPILIVVYNDAAYGAEVHHFAPMGWPT
HHEECCCEEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCC
STVQFPDTNIAELARALGMHAFTVRTQRDLESLREALSDRSCYPMLLDAKVNPAVRGGEW
CEEECCCCCHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCE
FDLAFEGH
EEEEEECC
>Mature Secondary Structure 
AKVYELLSRQLIGLGSDTIFGLVGSGNFFLAESLVTHGAKFVAARHEAGALAMADAWAR
CHHHHHHHHHHHCCCCCEEEEEEECCCEEEHHHHHHCCHHEEEECCCCCCCHHHHHHHH
LTQKVGICTVHMGPGFTNIITPLVEAAKARTPLLVLTADVSTGAVTSTFAIDIDALATAS
HHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEHHHHHCCC
GAIPERLWKNSTATQDLVRAYRRARYERRPVVFVMPQDIQAAESTAAVIAPEDNAYFSVI
CCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCCEEEEC
PDRSILEKIVSLVRDSRHPLILAGRGSVLAGARAGLEKLAEAIGALLATTANAHGLFAGN
CCHHHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
PWSIGIAGGFSSPGAVELFRQADLILAFGASLTVWTRRHGRLFPESCPIVQIDLDPAALH
CEEEEEECCCCCCCHHHHHHHCCEEEEECCEEEEEECCCCCCCCCCCCEEEEECCHHHHC
RQYPVTLACVGDARATAEALLELLPDKPVGQWRTPSTLQFIQEHSWINTPFEDASTENTI
CCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
DPRTLSLFLEQNLPPDRILVLDSGHFMAYPVMYLSVPDEKGFVFTQALQSIGFGLPNAIG
CCHHEEEEECCCCCCCEEEEEECCCEEEEEEEEEECCCCCCCHHHHHHHHHCCCCCHHHH
AAIAYPDRLTIAATGDGGLLLSLSELETVARYRLPILIVVYNDAAYGAEVHHFAPMGWPT
HHEECCCEEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCC
STVQFPDTNIAELARALGMHAFTVRTQRDLESLREALSDRSCYPMLLDAKVNPAVRGGEW
CEEECCCCCHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCE
FDLAFEGH
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11439112; 12358600 [H]