| Definition | Thermomicrobium roseum DSM 5159 plasmid unnamed, complete sequence. |
|---|---|
| Accession | NC_011961 |
| Length | 917,738 |
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The map label for this gene is menD [H]
Identifier: 221635667
GI number: 221635667
Start: 246249
End: 248000
Strand: Reverse
Name: menD [H]
Synonym: trd_A0261
Alternate gene names: 221635667
Gene position: 248000-246249 (Counterclockwise)
Preceding gene: 221635668
Following gene: 221635666
Centisome position: 27.02
GC content: 72.83
Gene sequence:
>1752_bases ATGGAGCATCGCACCGCGCTCGGCGCGACCGTCACTGCCTTCGTCACCGCACTGGCCGATGCCGGCCTCCGGCACGCCTG CTTCGCGCCGGGGTCGCGCTCCACACCGCTCGTCCTCGGACTGGCGCGCGAGCCGCGCATCCGCCTCTGGGACCATCTGG ACGAGCGTTCGGCTGGCTTTTTCGCCCTGGGGCTCGCCCGGGGACTCGACGAGCCGGTCGCCGTCGTGACCACCTCCGGA ACTGCCGCCGCCAACCTGCTCCCTGCCGTCGTCGAGGCGAACCTGGCCCAGGTCCCGCTGGTCGTCCTGACCGCCGACCG ACCGCCCGAGCTGCGCGACGCCGGTGCGCCGCAGACGATCGACCAGCTCCGGCTCTACGGGACGCACGTCAAGTGGTTCT CCGAACTCCCGCCGCCGGACGGCACGCCGCTCGTCCAGCGCGCGGCGGCGTCCGCTGCTCGTCGGGCCGTCGCGCTCGCC CGCGCCTTCCCGCCAGGGCCCGTCCACCTGAACCTGCCCTACCGCGAGCCGCTCGTCCCCCACTTCGGCCTGAGCATCGC CGCACCGGCCGGGGCTGTCCTGAGCGGAACACCGCAGCTGGACGAGCGCGAGCTGGCGGAGGTCGCGGCGTTACTGGCTC GACGCCGACGCGGCGTCGTATTGGCCGGGCCGCAGCCGGACCCCGCACTGGCTCCGGCGCTCGTCGAGCTGGCCAGCCTG CTCGGTTACCCGATCCTGGCCGATCCGCTGTCGCAGCTCCGGGCTGGCCCGCATCCGCGCGACCTCGTGCTCGATGCCTA CGATGCCGTCCTGCGGGAGCCGGAGGCGCTGCCGGCGCTCCAGCCGGAGCTCGTCCTGCGCGTCGGCGCGATCCCGACCT CGAAGCCGCTGCAGCTCGCGCTGGAGGCGTGGGAGAACACCGAGCAGATTCTTTTGGCTGCCGGAAACTGGCCGGACCCG GCGCACCGGGCCAGTTGGGTCCTCCAAGGTGCCCTGAGGCCGACTGTCCGCGCCCTGACCCGCGCCCTCGCGCTCGCCAC GCCGCGGCCGGACACCGACTGGCTCGCCCGTTGGCGGGCAGTCGATCGCGCGGCCCGGGCCGCGCTCGAGGCTGGGCTCG CCGAACTCGATGAGCCGTTCGAGGGCCGCGTCTTCCGCGAGCTGGCTGAACTCTTGCCGGACGGCGCAGCGCTCGTCGCC GGGAACAGCATGCCGGTCCGCGACCTGGACGCGTTTTTCCCCTCCGTTCCGCAGCGGGTGCGCATCTTCGCCAATCGCGG AGCCAACGGGATCGACGGCGTCACGTCGACTGCGCTCGGTATCGCCGCGGCCCGTACCGGCCCGACCGTCCTGGTGACCG GCGATCTGTCCTTCTACCATGACCTCACCGGGCTCCTGGCGGCACGGCGCCATCGCCTGCGCGCGACCATCGTGCTCCTC CACAACGACGGCGGCGGCATCTTTTCCTTTCTTCCCCAGGCGCACGAGGTACCGGAGCAGTTCGAGTTCCTGTTCGGCAC GCCGCACGGTCTCGATTTCCAGCACGCCGCCGCCCTCTTCGGGCTGGCCTATGCCCGTCCCACCGATCGCTCGAGCGTCC GGGACGCTCTGGCCGCAGCACTGGTCGCCGACCGGACCAGCATCGTCGAGCTGCGCAGCGACCGGCAGCGCAACGTCGTG CTCCACCAGGAGCTCTGGGCACGAGCGCGCGCCGCGGTGCGGGACGCGCTGGCCGCGGGAACCCGGCGATGA
Upstream 100 bases:
>100_bases TGGGTGCGGCATCGTCGCCGATTCCGATTCAGTGGCCGAATGGGCGGAAGCCGAAGCGAAGTTCCGGCCGATGCTCGAGG CGCTCGCGGGAGGCTGAGCG
Downstream 100 bases:
>100_bases GGGTGTGGGACGAAGAGCGGACGATCGCTGTCCGCGGCGTCACTTACCGCGTCTGTGCGGCCGGGCAGGGACCGCCGCTG GCCCTGCTGCACGGGTTCGC
Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase
Products: NA
Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]
Number of amino acids: Translated: 583; Mature: 583
Protein sequence:
>583_residues MEHRTALGATVTAFVTALADAGLRHACFAPGSRSTPLVLGLAREPRIRLWDHLDERSAGFFALGLARGLDEPVAVVTTSG TAAANLLPAVVEANLAQVPLVVLTADRPPELRDAGAPQTIDQLRLYGTHVKWFSELPPPDGTPLVQRAAASAARRAVALA RAFPPGPVHLNLPYREPLVPHFGLSIAAPAGAVLSGTPQLDERELAEVAALLARRRRGVVLAGPQPDPALAPALVELASL LGYPILADPLSQLRAGPHPRDLVLDAYDAVLREPEALPALQPELVLRVGAIPTSKPLQLALEAWENTEQILLAAGNWPDP AHRASWVLQGALRPTVRALTRALALATPRPDTDWLARWRAVDRAARAALEAGLAELDEPFEGRVFRELAELLPDGAALVA GNSMPVRDLDAFFPSVPQRVRIFANRGANGIDGVTSTALGIAAARTGPTVLVTGDLSFYHDLTGLLAARRHRLRATIVLL HNDGGGIFSFLPQAHEVPEQFEFLFGTPHGLDFQHAAALFGLAYARPTDRSSVRDALAAALVADRTSIVELRSDRQRNVV LHQELWARARAAVRDALAAGTRR
Sequences:
>Translated_583_residues MEHRTALGATVTAFVTALADAGLRHACFAPGSRSTPLVLGLAREPRIRLWDHLDERSAGFFALGLARGLDEPVAVVTTSG TAAANLLPAVVEANLAQVPLVVLTADRPPELRDAGAPQTIDQLRLYGTHVKWFSELPPPDGTPLVQRAAASAARRAVALA RAFPPGPVHLNLPYREPLVPHFGLSIAAPAGAVLSGTPQLDERELAEVAALLARRRRGVVLAGPQPDPALAPALVELASL LGYPILADPLSQLRAGPHPRDLVLDAYDAVLREPEALPALQPELVLRVGAIPTSKPLQLALEAWENTEQILLAAGNWPDP AHRASWVLQGALRPTVRALTRALALATPRPDTDWLARWRAVDRAARAALEAGLAELDEPFEGRVFRELAELLPDGAALVA GNSMPVRDLDAFFPSVPQRVRIFANRGANGIDGVTSTALGIAAARTGPTVLVTGDLSFYHDLTGLLAARRHRLRATIVLL HNDGGGIFSFLPQAHEVPEQFEFLFGTPHGLDFQHAAALFGLAYARPTDRSSVRDALAAALVADRTSIVELRSDRQRNVV LHQELWARARAAVRDALAAGTRR >Mature_583_residues MEHRTALGATVTAFVTALADAGLRHACFAPGSRSTPLVLGLAREPRIRLWDHLDERSAGFFALGLARGLDEPVAVVTTSG TAAANLLPAVVEANLAQVPLVVLTADRPPELRDAGAPQTIDQLRLYGTHVKWFSELPPPDGTPLVQRAAASAARRAVALA RAFPPGPVHLNLPYREPLVPHFGLSIAAPAGAVLSGTPQLDERELAEVAALLARRRRGVVLAGPQPDPALAPALVELASL LGYPILADPLSQLRAGPHPRDLVLDAYDAVLREPEALPALQPELVLRVGAIPTSKPLQLALEAWENTEQILLAAGNWPDP AHRASWVLQGALRPTVRALTRALALATPRPDTDWLARWRAVDRAARAALEAGLAELDEPFEGRVFRELAELLPDGAALVA GNSMPVRDLDAFFPSVPQRVRIFANRGANGIDGVTSTALGIAAARTGPTVLVTGDLSFYHDLTGLLAARRHRLRATIVLL HNDGGGIFSFLPQAHEVPEQFEFLFGTPHGLDFQHAAALFGLAYARPTDRSSVRDALAAALVADRTSIVELRSDRQRNVV LHQELWARARAAVRDALAAGTRR
Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca
COG id: COG1165
COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]
Homologues:
Organism=Escherichia coli, GI1788599, Length=551, Percent_Identity=34.1197822141561, Blast_Score=237, Evalue=2e-63,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004433 - InterPro: IPR012001 - InterPro: IPR011766 [H]
Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]
EC number: =2.2.1.9 [H]
Molecular weight: Translated: 62363; Mature: 62363
Theoretical pI: Translated: 7.29; Mature: 7.29
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 0.3 %Met (Translated Protein) 0.5 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 0.3 %Met (Mature Protein) 0.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MEHRTALGATVTAFVTALADAGLRHACFAPGSRSTPLVLGLAREPRIRLWDHLDERSAGF CCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCCE FALGLARGLDEPVAVVTTSGTAAANLLPAVVEANLAQVPLVVLTADRPPELRDAGAPQTI EEHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCHHHCCEEEEECCCCCCHHCCCCCHHH DQLRLYGTHVKWFSELPPPDGTPLVQRAAASAARRAVALARAFPPGPVHLNLPYREPLVP HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC HFGLSIAAPAGAVLSGTPQLDERELAEVAALLARRRRGVVLAGPQPDPALAPALVELASL CCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH LGYPILADPLSQLRAGPHPRDLVLDAYDAVLREPEALPALQPELVLRVGAIPTSKPLQLA HCCCHHHCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCCCCCHHHHH LEAWENTEQILLAAGNWPDPAHRASWVLQGALRPTVRALTRALALATPRPDTDWLARWRA HHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH VDRAARAALEAGLAELDEPFEGRVFRELAELLPDGAALVAGNSMPVRDLDAFFPSVPQRV HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCHHH RIFANRGANGIDGVTSTALGIAAARTGPTVLVTGDLSFYHDLTGLLAARRHRLRATIVLL HHHHCCCCCCCCCHHHHHHHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHCEEEEEEEE HNDGGGIFSFLPQAHEVPEQFEFLFGTPHGLDFQHAAALFGLAYARPTDRSSVRDALAAA ECCCCCHHHHCCCHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH LVADRTSIVELRSDRQRNVVLHQELWARARAAVRDALAAGTRR HHHCCHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCC >Mature Secondary Structure MEHRTALGATVTAFVTALADAGLRHACFAPGSRSTPLVLGLAREPRIRLWDHLDERSAGF CCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCCE FALGLARGLDEPVAVVTTSGTAAANLLPAVVEANLAQVPLVVLTADRPPELRDAGAPQTI EEHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCHHHCCEEEEECCCCCCHHCCCCCHHH DQLRLYGTHVKWFSELPPPDGTPLVQRAAASAARRAVALARAFPPGPVHLNLPYREPLVP HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC HFGLSIAAPAGAVLSGTPQLDERELAEVAALLARRRRGVVLAGPQPDPALAPALVELASL CCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH LGYPILADPLSQLRAGPHPRDLVLDAYDAVLREPEALPALQPELVLRVGAIPTSKPLQLA HCCCHHHCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCCCCCHHHHH LEAWENTEQILLAAGNWPDPAHRASWVLQGALRPTVRALTRALALATPRPDTDWLARWRA HHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH VDRAARAALEAGLAELDEPFEGRVFRELAELLPDGAALVAGNSMPVRDLDAFFPSVPQRV HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCHHH RIFANRGANGIDGVTSTALGIAAARTGPTVLVTGDLSFYHDLTGLLAARRHRLRATIVLL HHHHCCCCCCCCCHHHHHHHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHCEEEEEEEE HNDGGGIFSFLPQAHEVPEQFEFLFGTPHGLDFQHAAALFGLAYARPTDRSSVRDALAAA ECCCCCHHHHCCCHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH LVADRTSIVELRSDRQRNVVLHQELWARARAAVRDALAAGTRR HHHCCHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA