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The map label for this gene is menF [H]

Identifier: 221635668

GI number: 221635668

Start: 248004

End: 249389

Strand: Reverse

Name: menF [H]

Synonym: trd_A0262

Alternate gene names: 221635668

Gene position: 249389-248004 (Counterclockwise)

Preceding gene: 221635669

Following gene: 221635667

Centisome position: 27.17

GC content: 72.8

Gene sequence:

>1386_bases
ATGAGCTGCCGAGTGCTCGATGCTCCCCAGGCTACCCTCCAGGAACTCTGGCCGCTGGCCGAGCGGCAGGCCCGCGCGCA
CGATCGCCCAGTGATCCTGAGCTGCGCGGAGCACTGCGAACCGGTCGACCCGATCGCGCTCTTCGCCCGCGCGGCCAGCC
ACCTCCCGCGCGTCCTCTGGCTCGTCCCCGAACGAGAGGAGGCGCTGGTTGGGCTGGCCGGCCAGCGCCTGCCGCTCCAG
TCGGTGACGAGCCCGAGTCTCCCCCTGCCAGCTCGCCTCGCCGCTGCCTGGCGGGCACTGGCTCAGGACGCGCTGGCCGA
GGGACCGCTCCCACCGCTGGCTGTCGCCGGGCTCGCCTTCGATCCGGCGGACGAGCGCCCCGATCCCTGGTGGCAGCCGT
TCGGTCAGGGCCAGCTTCTCCTGCCGCGCCTGCTCTACCGGCAACAGGGGGCGCTCGCCTGCCGGTCCGTGCAAGCGGCT
GTGTTCCCCGGCGAGCGCGAACCGCGCGCGCTCCTGACCTGGCCGACGAGTTCGCTGCCGACTCCATCGGCCGCTCCTCG
CTCGGTCGTCCAGCACGAACTGGTCCGACCGGACGACTGGCGGCGCATGATCCAGCACGCGCTGGCCGCGATCGCTGCTG
GCGATCTCCGCAAGGTCGTCCTGGCCCGCGCCCGCGAGCTGGTGGCTGCCGACCCCTTCCCGCTCGAGCAGGCCCTGCGG
GAGCTTGCGGCCCGCTATCCGAGTTGCACCGTGTTCGCCATCGGGATCGGCGAGACCGTCTTCCTGGGGGCGAGCCCGGA
GCGACTCGCCCGGGTCGCCCACGGCACCGTCGCCACCGTCGCCCTGGCCGGCTCGCGCCCGCGCGCGAGCTCGCCGGAAC
AGGACCACCGCGCGGCCGAGGAACTGCGCCGGAGCGACAAGGACCGCCACGAGCACGCGCTGGTCGTCGAGGCGATCCGC
TCGGCGCTCGCCCCGCTCTGCACCAGCCTGAGCATCCCGGAAGAACCAGTGGTCGTTTCCATGGCCAACGTGCATCACTT
GGCCACGCCGATCAGCGGCATCCTCCACGATGGCGCCAGTGTGCTCGACGTCGTGGCCCGCCTCCACCCGACGCCAGCCG
TCGGCGGGACGCCGCGCGAGGCAGCGCTCGCTTTCATCCGCCAGCGCGAGCCGGTTCCCCGTGGCTGGTACGCCGGTGCG
CTCGGCTGGATCGAAGCGAGCGGTGACGGCGAGATCGTCGTCGGCCTGCGCTCCGGCCTGGTGCGCAGCCAGCAGGCGCG
ACTCTACGCTGGGTGCGGCATCGTCGCCGATTCCGATTCAGTGGCCGAATGGGCGGAAGCCGAAGCGAAGTTCCGGCCGA
TGCTCGAGGCGCTCGCGGGAGGCTGA

Upstream 100 bases:

>100_bases
GCTCGCCGGCCGATCTCGCCGAAGCCGCCCCGGAACTCCGCCGCCTGCTCCGTCGCCTGGTCGGCCTCCCCGACCGCTCG
CTGGACGAGGATGGAACCCT

Downstream 100 bases:

>100_bases
GCGATGGAGCATCGCACCGCGCTCGGCGCGACCGTCACTGCCTTCGTCACCGCACTGGCCGATGCCGGCCTCCGGCACGC
CTGCTTCGCGCCGGGGTCGC

Product: salicylate biosynthesis isochorismate synthase (Isochorismate mutase)

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSCRVLDAPQATLQELWPLAERQARAHDRPVILSCAEHCEPVDPIALFARAASHLPRVLWLVPEREEALVGLAGQRLPLQ
SVTSPSLPLPARLAAAWRALAQDALAEGPLPPLAVAGLAFDPADERPDPWWQPFGQGQLLLPRLLYRQQGALACRSVQAA
VFPGEREPRALLTWPTSSLPTPSAAPRSVVQHELVRPDDWRRMIQHALAAIAAGDLRKVVLARARELVAADPFPLEQALR
ELAARYPSCTVFAIGIGETVFLGASPERLARVAHGTVATVALAGSRPRASSPEQDHRAAEELRRSDKDRHEHALVVEAIR
SALAPLCTSLSIPEEPVVVSMANVHHLATPISGILHDGASVLDVVARLHPTPAVGGTPREAALAFIRQREPVPRGWYAGA
LGWIEASGDGEIVVGLRSGLVRSQQARLYAGCGIVADSDSVAEWAEAEAKFRPMLEALAGG

Sequences:

>Translated_461_residues
MSCRVLDAPQATLQELWPLAERQARAHDRPVILSCAEHCEPVDPIALFARAASHLPRVLWLVPEREEALVGLAGQRLPLQ
SVTSPSLPLPARLAAAWRALAQDALAEGPLPPLAVAGLAFDPADERPDPWWQPFGQGQLLLPRLLYRQQGALACRSVQAA
VFPGEREPRALLTWPTSSLPTPSAAPRSVVQHELVRPDDWRRMIQHALAAIAAGDLRKVVLARARELVAADPFPLEQALR
ELAARYPSCTVFAIGIGETVFLGASPERLARVAHGTVATVALAGSRPRASSPEQDHRAAEELRRSDKDRHEHALVVEAIR
SALAPLCTSLSIPEEPVVVSMANVHHLATPISGILHDGASVLDVVARLHPTPAVGGTPREAALAFIRQREPVPRGWYAGA
LGWIEASGDGEIVVGLRSGLVRSQQARLYAGCGIVADSDSVAEWAEAEAKFRPMLEALAGG
>Mature_460_residues
SCRVLDAPQATLQELWPLAERQARAHDRPVILSCAEHCEPVDPIALFARAASHLPRVLWLVPEREEALVGLAGQRLPLQS
VTSPSLPLPARLAAAWRALAQDALAEGPLPPLAVAGLAFDPADERPDPWWQPFGQGQLLLPRLLYRQQGALACRSVQAAV
FPGEREPRALLTWPTSSLPTPSAAPRSVVQHELVRPDDWRRMIQHALAAIAAGDLRKVVLARARELVAADPFPLEQALRE
LAARYPSCTVFAIGIGETVFLGASPERLARVAHGTVATVALAGSRPRASSPEQDHRAAEELRRSDKDRHEHALVVEAIRS
ALAPLCTSLSIPEEPVVVSMANVHHLATPISGILHDGASVLDVVARLHPTPAVGGTPREAALAFIRQREPVPRGWYAGAL
GWIEASGDGEIVVGLRSGLVRSQQARLYAGCGIVADSDSVAEWAEAEAKFRPMLEALAGG

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=410, Percent_Identity=30, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI87082077, Length=344, Percent_Identity=33.4302325581395, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1788114, Length=262, Percent_Identity=29.0076335877863, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787518, Length=322, Percent_Identity=27.639751552795, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6320935, Length=290, Percent_Identity=30.3448275862069, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 49516; Mature: 49385

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCRVLDAPQATLQELWPLAERQARAHDRPVILSCAEHCEPVDPIALFARAASHLPRVLW
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHCCEEEE
LVPEREEALVGLAGQRLPLQSVTSPSLPLPARLAAAWRALAQDALAEGPLPPLAVAGLAF
ECCCHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEE
DPADERPDPWWQPFGQGQLLLPRLLYRQQGALACRSVQAAVFPGEREPRALLTWPTSSLP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCC
TPSAAPRSVVQHELVRPDDWRRMIQHALAAIAAGDLRKVVLARARELVAADPFPLEQALR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ELAARYPSCTVFAIGIGETVFLGASPERLARVAHGTVATVALAGSRPRASSPEQDHRAAE
HHHHHCCCCEEEEEECCCEEEECCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHH
ELRRSDKDRHEHALVVEAIRSALAPLCTSLSIPEEPVVVSMANVHHLATPISGILHDGAS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCHH
VLDVVARLHPTPAVGGTPREAALAFIRQREPVPRGWYAGALGWIEASGDGEIVVGLRSGL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCEEEEEHHCC
VRSQQARLYAGCGIVADSDSVAEWAEAEAKFRPMLEALAGG
HHHHHHHEEECCCEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SCRVLDAPQATLQELWPLAERQARAHDRPVILSCAEHCEPVDPIALFARAASHLPRVLW
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHCCEEEE
LVPEREEALVGLAGQRLPLQSVTSPSLPLPARLAAAWRALAQDALAEGPLPPLAVAGLAF
ECCCHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEE
DPADERPDPWWQPFGQGQLLLPRLLYRQQGALACRSVQAAVFPGEREPRALLTWPTSSLP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCC
TPSAAPRSVVQHELVRPDDWRRMIQHALAAIAAGDLRKVVLARARELVAADPFPLEQALR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ELAARYPSCTVFAIGIGETVFLGASPERLARVAHGTVATVALAGSRPRASSPEQDHRAAE
HHHHHCCCCEEEEEECCCEEEECCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHH
ELRRSDKDRHEHALVVEAIRSALAPLCTSLSIPEEPVVVSMANVHHLATPISGILHDGAS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCHH
VLDVVARLHPTPAVGGTPREAALAFIRQREPVPRGWYAGALGWIEASGDGEIVVGLRSGL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCEEEEEHHCC
VRSQQARLYAGCGIVADSDSVAEWAEAEAKFRPMLEALAGG
HHHHHHHEEECCCEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]