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The map label for this gene is trpB2 [H]

Identifier: 221635606

GI number: 221635606

Start: 181915

End: 183282

Strand: Reverse

Name: trpB2 [H]

Synonym: trd_A0200

Alternate gene names: 221635606

Gene position: 183282-181915 (Counterclockwise)

Preceding gene: 221635607

Following gene: 221635603

Centisome position: 19.97

GC content: 66.23

Gene sequence:

>1368_bases
GTGAGCGCAGCAGAGACCGTCAAGTTCGTGTTGACCGAGGCCGATCTCCCAACGCACTGGTACAACCTGGCCGCCGATCT
CCCCACGCTTCCCCCACCGCCCATCCACCCAGGGACCGGGGAACCGGTGGGTCCGGCCGACCTCGAACCCGTCTTTCCGA
TGGAACTCATCCTCCAGGAAGTGAGCCGCGAGCGCTGGATCGCGATCCCCGGGCCGGTTCTCGACGTCTACCGACTCTGG
CGACCGACGCCACTCTTCCGAGCCCGTCGGCTCGAGCGCGTGCTCGGTACACCAGCGCGCATCTACTACAAGTACGAAGG
GGTCAGCCCGGCCGGGAGTCATAAGCCGAACACGGCGGTCGCCCAGGCCTACTACAACAAGCAGGCCGGCGTGAAGCGGC
TCACCACCGAGACCGGAGCGGGTCAGTGGGGAAGCTCGCTCGCCATGGCCTGCGCCTTCTTCGGTATGGAACTCAAGGTG
TATATGGTGCGGGCCAGCTATCTCCAGAAGCCGTACCGGCGTATCCTGATGGAGACCTGGGGAGCCCAGTGCGTCCCCAG
CCCCAGTCCCGACACCGCGTCCGGCCGGCGCTTCTACGAGCAGGATCCCGAGCATCCGGGAAGCCTCGGAATCGCCATTT
CCGAGGCGGTCGAGGACGCAGCCTCGCGCGCGGACACCAAGTACTCGCTCGGGAGCGTGCTCAACCACGTGCTCCTGCAC
CAGACGGTCATCGGGCTGGAGGCGCTCCGGCAGTTCGAGCTGGCCGGTGACTACCCGGACGTCATCTTTGGCTGTGTCGG
GGGCGGTTCCAACTTCGCTGGTCTCACTTTTCCCTTCGTGCGGGACAAGCTTCAGGGGAAGGACGTGCGCATCGTCGCCG
TCGAACCGGCCGCCTGCCCGTCGCTCACCCGCGGCGTCTATACCTACGACTTCGGCGATACGGCACGGACGACGCCGCTC
CTCAAGATGCATACGCTCGGCCACACCTTCATCCCTGCCCGGATCCACGCCGGTGGCTTGCGCTACCATGGGATGGCACC
ACTGGTCAGCCACCTCTACGAGCACGGACTGATCGAGGCGATCGCGCTCCCGCAGACCAAGGTGTTCGAGGGAGCCGTTC
TGTTCGCCCGTGCCGAGGGGATCATTCCCGCTCCCGAATCGGCACATGCCGTGCGCGCTGCGATCGACGAGGCCCGCCGC
TGCGCCGAGACTGGCGAAGAGAAGGCGATCCTGATCGGGCTGAGCGGGCACGGCCACTTCGATCTGGCAGCCTACGAGGC
CTACCTCGCCGGGAAGCTGGAAGACTACGAGTATCCCGAGGAAGCAGTCCGCCAGGCGCTCGCCGAGCTGCCGAAGGTCA
GCGGCTGA

Upstream 100 bases:

>100_bases
GCCGGGAAGGCGGCTGCAGGACGATCTCTTGCAGGATCCGGTCGTTTCGTTACAATGCCTGCAGTGACGAGCGGCACAAC
AGGCAAAGCGGAGGACGAGG

Downstream 100 bases:

>100_bases
CCACCGCGACGAGCGACTTGCGACCGCACCCCGCTGCTCCCGTTGCACGATCGGGGCGGCGGGGTGTTTTTCGGGGATAC
CTGCTCGCGCTGGCAGCACC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSAAETVKFVLTEADLPTHWYNLAADLPTLPPPPIHPGTGEPVGPADLEPVFPMELILQEVSRERWIAIPGPVLDVYRLW
RPTPLFRARRLERVLGTPARIYYKYEGVSPAGSHKPNTAVAQAYYNKQAGVKRLTTETGAGQWGSSLAMACAFFGMELKV
YMVRASYLQKPYRRILMETWGAQCVPSPSPDTASGRRFYEQDPEHPGSLGIAISEAVEDAASRADTKYSLGSVLNHVLLH
QTVIGLEALRQFELAGDYPDVIFGCVGGGSNFAGLTFPFVRDKLQGKDVRIVAVEPAACPSLTRGVYTYDFGDTARTTPL
LKMHTLGHTFIPARIHAGGLRYHGMAPLVSHLYEHGLIEAIALPQTKVFEGAVLFARAEGIIPAPESAHAVRAAIDEARR
CAETGEEKAILIGLSGHGHFDLAAYEAYLAGKLEDYEYPEEAVRQALAELPKVSG

Sequences:

>Translated_455_residues
MSAAETVKFVLTEADLPTHWYNLAADLPTLPPPPIHPGTGEPVGPADLEPVFPMELILQEVSRERWIAIPGPVLDVYRLW
RPTPLFRARRLERVLGTPARIYYKYEGVSPAGSHKPNTAVAQAYYNKQAGVKRLTTETGAGQWGSSLAMACAFFGMELKV
YMVRASYLQKPYRRILMETWGAQCVPSPSPDTASGRRFYEQDPEHPGSLGIAISEAVEDAASRADTKYSLGSVLNHVLLH
QTVIGLEALRQFELAGDYPDVIFGCVGGGSNFAGLTFPFVRDKLQGKDVRIVAVEPAACPSLTRGVYTYDFGDTARTTPL
LKMHTLGHTFIPARIHAGGLRYHGMAPLVSHLYEHGLIEAIALPQTKVFEGAVLFARAEGIIPAPESAHAVRAAIDEARR
CAETGEEKAILIGLSGHGHFDLAAYEAYLAGKLEDYEYPEEAVRQALAELPKVSG
>Mature_454_residues
SAAETVKFVLTEADLPTHWYNLAADLPTLPPPPIHPGTGEPVGPADLEPVFPMELILQEVSRERWIAIPGPVLDVYRLWR
PTPLFRARRLERVLGTPARIYYKYEGVSPAGSHKPNTAVAQAYYNKQAGVKRLTTETGAGQWGSSLAMACAFFGMELKVY
MVRASYLQKPYRRILMETWGAQCVPSPSPDTASGRRFYEQDPEHPGSLGIAISEAVEDAASRADTKYSLGSVLNHVLLHQ
TVIGLEALRQFELAGDYPDVIFGCVGGGSNFAGLTFPFVRDKLQGKDVRIVAVEPAACPSLTRGVYTYDFGDTARTTPLL
KMHTLGHTFIPARIHAGGLRYHGMAPLVSHLYEHGLIEAIALPQTKVFEGAVLFARAEGIIPAPESAHAVRAAIDEARRC
AETGEEKAILIGLSGHGHFDLAAYEAYLAGKLEDYEYPEEAVRQALAELPKVSG

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=361, Percent_Identity=29.3628808864266, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321412, Length=372, Percent_Identity=30.9139784946237, Blast_Score=123, Evalue=6e-29,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 49647; Mature: 49516

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAETVKFVLTEADLPTHWYNLAADLPTLPPPPIHPGTGEPVGPADLEPVFPMELILQE
CCHHHEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VSRERWIAIPGPVLDVYRLWRPTPLFRARRLERVLGTPARIYYKYEGVSPAGSHKPNTAV
HCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH
AQAYYNKQAGVKRLTTETGAGQWGSSLAMACAFFGMELKVYMVRASYLQKPYRRILMETW
HHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHC
GAQCVPSPSPDTASGRRFYEQDPEHPGSLGIAISEAVEDAASRADTKYSLGSVLNHVLLH
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QTVIGLEALRQFELAGDYPDVIFGCVGGGSNFAGLTFPFVRDKLQGKDVRIVAVEPAACP
HHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCC
SLTRGVYTYDFGDTARTTPLLKMHTLGHTFIPARIHAGGLRYHGMAPLVSHLYEHGLIEA
HHHCCCEEEECCCCCCCCCEEEEHHCCCEEECCEEECCCCEECCHHHHHHHHHHCCCHHH
IALPQTKVFEGAVLFARAEGIIPAPESAHAVRAAIDEARRCAETGEEKAILIGLSGHGHF
HCCCCCHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCE
DLAAYEAYLAGKLEDYEYPEEAVRQALAELPKVSG
EHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAAETVKFVLTEADLPTHWYNLAADLPTLPPPPIHPGTGEPVGPADLEPVFPMELILQE
CHHHEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VSRERWIAIPGPVLDVYRLWRPTPLFRARRLERVLGTPARIYYKYEGVSPAGSHKPNTAV
HCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH
AQAYYNKQAGVKRLTTETGAGQWGSSLAMACAFFGMELKVYMVRASYLQKPYRRILMETW
HHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHC
GAQCVPSPSPDTASGRRFYEQDPEHPGSLGIAISEAVEDAASRADTKYSLGSVLNHVLLH
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QTVIGLEALRQFELAGDYPDVIFGCVGGGSNFAGLTFPFVRDKLQGKDVRIVAVEPAACP
HHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCC
SLTRGVYTYDFGDTARTTPLLKMHTLGHTFIPARIHAGGLRYHGMAPLVSHLYEHGLIEA
HHHCCCEEEECCCCCCCCCEEEEHHCCCEEECCEEECCCCEECCHHHHHHHHHHCCCHHH
IALPQTKVFEGAVLFARAEGIIPAPESAHAVRAAIDEARRCAETGEEKAILIGLSGHGHF
HCCCCCHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCE
DLAAYEAYLAGKLEDYEYPEEAVRQALAELPKVSG
EHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]