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The map label for this gene is liaR [H]

Identifier: 221635561

GI number: 221635561

Start: 143988

End: 146300

Strand: Reverse

Name: liaR [H]

Synonym: trd_A0155

Alternate gene names: 221635561

Gene position: 146300-143988 (Counterclockwise)

Preceding gene: 221635562

Following gene: 221635560

Centisome position: 15.94

GC content: 71.21

Gene sequence:

>2313_bases
ATGCCCGAGCGAGCGATCCGCATCCTCCTCGTCGATGACCATGCCCTCTTCCGGCAGGGTCTCCGCCAGTTGCTCGCCAC
CACTCCCGATATCGTCGTTGTCGGGGAAGCGGGATCAGCGAGCGAAGCCTTCGAGCTGGTACCCCAGCTCGCTCCCGATG
TCGTGCTCATGGATATCGCGATGCCGGGACTCGACGGCATCGCTGCCACCGCTGCGCTCCGCCAGCGCTTTCCCACTCTC
CGCGTCGTCATGCTGACCATGTACGAGCCAACGACCTCTGGCGAGGCAGCACGCGCTGCCGGCGCCATCGCCTACGTGGT
GAAGAGCAGCCGGCCAGAGGAACTCTTCGCTGCCATCCGCGCAGCCGCCAACGGCGCTATCCCGCCCAACGGGGAGGTGC
GAGCAGGGCAGTCTCCCCCACCTCCAGGCCGGGATACTGCTGATCGGGTCGCACAGCTCGAGCAACGTCTCGCCTGGCTG
GAAGCCCAGCTCGCCCAACTCGGCGTTCGTGAGCCACTGCCACCATCCCACGCTCCAGCAGAACCGGTCATGAGCGCTCC
GCCAGCCGACCAGCCGATCGCTCCCTCTCCGCCGGTCGGAGCAGTGTCCCGGCACCCGGTGTGGCAGCCCAGCATTGGCA
CCGCGCGAGCGGCGGATACCATGCCGACCCCCCGATCCGTTTCCCCACAACCATTGCCCGTCGCGCTCACGCAGCTGGCG
AACAGCCTTCTCCTGGGTATCCTCGTCGTCGGCGAACTCGTCGCGCTCGCCAGCGCCCTCTTGCTCTTTCCCTCGCCGAT
CCTCGCGCGCTGGATCACCCTGGGAGCCGGGCAACTCGCGAGCCTGGCTCTCATCACGCTCGTCGCGCTCGCTCCGCAGC
GCCGGAGCGAGGGCCAAATCGTGCTGCTCGTCGCGCTCACCGCAGCGATGGCCTGGAGCCTCCTCACCGCACGATCGGCG
CCGGTCGCGCTCCAGGCGGCTGCGCTCGCCGGGACGAGCAGCCTCGCGCTCGCCGCGCTGGCACTCTCCTGGCAGCGCCG
CTTCCTTCTGGCAGCCGCACTCTCCCTGGCACTGATCACGGTCCTGCCATCCTTACTCGCGATCATCCCCGGTGCACTCG
CCCCGTTGTGGGTCACCGGAACCGTCGCCCTGGCGAGCGGTCTCGCCTGGTCACGCCTCCGCCGGCCGAGCGATCCGGTC
GCCTGGGAGTGGCTGCCGCTGGCGCCGCTCGCGCTCGGCACCCCGCTCCTCCTGGTCTGGCTGGAGAGCGGCGGTCGGCC
AGCGCTTCTCCTCGCTGCCCCGTGGATCGCTGCCTTCCCGCCGCTCGTCGCAGCCGGGAAGGCGCAACGACCAGTGCTGC
GAGCGCTCCTCGCACTCCACGGAGCGCTCGCCCTCGGCACGGTCGGCTGGCTTTTCCGCACGGCTGCCCACAGCGAGCAA
GCTGCTGCGCTCGCCGTGCTCGCCGTGCTGGCGAGCGCACTGGCGCTCCTCCTCTGGTGGAGCGGGCAACGGGCCGGTGC
CAGCACCGCCCCCGCAGTTGTCCCCGCCGCGTTCGCTGGCCTCTCGCTCGCCCTCGCCGCTGCCCGACACCCAGAGCCGG
TGCTCGCCCCGCTCGTCTGGTGCACGCTGGGACTCGTGCTCGCGGCGCTGCCCTCCCGGTGCACCTGGTCGACGTGGATC
GCGCTCGCGCTCTTCGCCGGCGGGTGCGGCGCGGCGCTCCTCGCTGTCCTTCGCTCTCCCGACTGGCAGCGCTGGAGCCT
CCTCGTCCTGGTCCTCGGCACAGCGACGATGACCGGTCCTTTCCTCGCTCCTCGCTGGTGGTTCGTCACTGGCTGGCTCG
GCAGCGGCGTGCTCGCGCTCGCCGCACTCCTGGCGCTGCGACTCACTGGCCTGGTGGAGATCGCGGCACTGAGCGGCCTG
GCACTGGGTACCCTCGTCGTGACCCGGTACATCGTCCCCCGGCTCGATCATCCGACCGGTGCCTGGGTGTGGCTTCCGAC
TCTCGGTGCCAGCGCCAGCGCACTGGCCAGCGCGCTGACCGGGCCACTCTCGCCGGGACGACTCGGCATCGCGCTCCAGC
CAGCGGTCTCCGCGACGAGCGAGCCGGTCATCGCGGCCAGCATGCTCGTCGCTGCTGCGCTGGCCGCCGGTCGCCTGCTC
GGTCGCCGCTGGCGCTGGGCCGCGATCGCGATCGCTCTGCTCCTGATCGCGTATGTTCTCCCAGCGGTCGTGCCGGACGC
CGCGCTCGTCCTGAGCTGGTCCGCGCTGGCCGTCGCACTCGCGCACGCGCTCGGCATCCGCCTCCGGCCCTGA

Upstream 100 bases:

>100_bases
CTGGCGCGACAGGATGCGAGCGCGGTAGCATCGACTCGACAGTGCGGAACCGGCCCTGCCGCGGTGCCAGTGTGGACCGC
CAGGGAGGAGAAAGCTGGCG

Downstream 100 bases:

>100_bases
TGCTGTCTCGCCCCCGAGCTGCACCGCCGAGGAGAGGTGTTCCTGAGGAACGGAGAACAGCGAGAGCCAGCGGCTGTTCC
CGCGCTGCTCGAGCCGGGCC

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 770; Mature: 769

Protein sequence:

>770_residues
MPERAIRILLVDDHALFRQGLRQLLATTPDIVVVGEAGSASEAFELVPQLAPDVVLMDIAMPGLDGIAATAALRQRFPTL
RVVMLTMYEPTTSGEAARAAGAIAYVVKSSRPEELFAAIRAAANGAIPPNGEVRAGQSPPPPGRDTADRVAQLEQRLAWL
EAQLAQLGVREPLPPSHAPAEPVMSAPPADQPIAPSPPVGAVSRHPVWQPSIGTARAADTMPTPRSVSPQPLPVALTQLA
NSLLLGILVVGELVALASALLLFPSPILARWITLGAGQLASLALITLVALAPQRRSEGQIVLLVALTAAMAWSLLTARSA
PVALQAAALAGTSSLALAALALSWQRRFLLAAALSLALITVLPSLLAIIPGALAPLWVTGTVALASGLAWSRLRRPSDPV
AWEWLPLAPLALGTPLLLVWLESGGRPALLLAAPWIAAFPPLVAAGKAQRPVLRALLALHGALALGTVGWLFRTAAHSEQ
AAALAVLAVLASALALLLWWSGQRAGASTAPAVVPAAFAGLSLALAAARHPEPVLAPLVWCTLGLVLAALPSRCTWSTWI
ALALFAGGCGAALLAVLRSPDWQRWSLLVLVLGTATMTGPFLAPRWWFVTGWLGSGVLALAALLALRLTGLVEIAALSGL
ALGTLVVTRYIVPRLDHPTGAWVWLPTLGASASALASALTGPLSPGRLGIALQPAVSATSEPVIAASMLVAAALAAGRLL
GRRWRWAAIAIALLLIAYVLPAVVPDAALVLSWSALAVALAHALGIRLRP

Sequences:

>Translated_770_residues
MPERAIRILLVDDHALFRQGLRQLLATTPDIVVVGEAGSASEAFELVPQLAPDVVLMDIAMPGLDGIAATAALRQRFPTL
RVVMLTMYEPTTSGEAARAAGAIAYVVKSSRPEELFAAIRAAANGAIPPNGEVRAGQSPPPPGRDTADRVAQLEQRLAWL
EAQLAQLGVREPLPPSHAPAEPVMSAPPADQPIAPSPPVGAVSRHPVWQPSIGTARAADTMPTPRSVSPQPLPVALTQLA
NSLLLGILVVGELVALASALLLFPSPILARWITLGAGQLASLALITLVALAPQRRSEGQIVLLVALTAAMAWSLLTARSA
PVALQAAALAGTSSLALAALALSWQRRFLLAAALSLALITVLPSLLAIIPGALAPLWVTGTVALASGLAWSRLRRPSDPV
AWEWLPLAPLALGTPLLLVWLESGGRPALLLAAPWIAAFPPLVAAGKAQRPVLRALLALHGALALGTVGWLFRTAAHSEQ
AAALAVLAVLASALALLLWWSGQRAGASTAPAVVPAAFAGLSLALAAARHPEPVLAPLVWCTLGLVLAALPSRCTWSTWI
ALALFAGGCGAALLAVLRSPDWQRWSLLVLVLGTATMTGPFLAPRWWFVTGWLGSGVLALAALLALRLTGLVEIAALSGL
ALGTLVVTRYIVPRLDHPTGAWVWLPTLGASASALASALTGPLSPGRLGIALQPAVSATSEPVIAASMLVAAALAAGRLL
GRRWRWAAIAIALLLIAYVLPAVVPDAALVLSWSALAVALAHALGIRLRP
>Mature_769_residues
PERAIRILLVDDHALFRQGLRQLLATTPDIVVVGEAGSASEAFELVPQLAPDVVLMDIAMPGLDGIAATAALRQRFPTLR
VVMLTMYEPTTSGEAARAAGAIAYVVKSSRPEELFAAIRAAANGAIPPNGEVRAGQSPPPPGRDTADRVAQLEQRLAWLE
AQLAQLGVREPLPPSHAPAEPVMSAPPADQPIAPSPPVGAVSRHPVWQPSIGTARAADTMPTPRSVSPQPLPVALTQLAN
SLLLGILVVGELVALASALLLFPSPILARWITLGAGQLASLALITLVALAPQRRSEGQIVLLVALTAAMAWSLLTARSAP
VALQAAALAGTSSLALAALALSWQRRFLLAAALSLALITVLPSLLAIIPGALAPLWVTGTVALASGLAWSRLRRPSDPVA
WEWLPLAPLALGTPLLLVWLESGGRPALLLAAPWIAAFPPLVAAGKAQRPVLRALLALHGALALGTVGWLFRTAAHSEQA
AALAVLAVLASALALLLWWSGQRAGASTAPAVVPAAFAGLSLALAAARHPEPVLAPLVWCTLGLVLAALPSRCTWSTWIA
LALFAGGCGAALLAVLRSPDWQRWSLLVLVLGTATMTGPFLAPRWWFVTGWLGSGVLALAALLALRLTGLVEIAALSGLA
LGTLVVTRYIVPRLDHPTGAWVWLPTLGASASALASALTGPLSPGRLGIALQPAVSATSEPVIAASMLVAAALAAGRLLG
RRWRWAAIAIALLLIAYVLPAVVPDAALVLSWSALAVALAHALGIRLRP

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=127, Percent_Identity=40.9448818897638, Blast_Score=93, Evalue=8e-20,
Organism=Escherichia coli, GI1788222, Length=120, Percent_Identity=38.3333333333333, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787473, Length=120, Percent_Identity=37.5, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790102, Length=153, Percent_Identity=32.6797385620915, Blast_Score=73, Evalue=8e-14,
Organism=Escherichia coli, GI1788712, Length=117, Percent_Identity=31.6239316239316, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 79545; Mature: 79414

Theoretical pI: Translated: 11.52; Mature: 11.52

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERAIRILLVDDHALFRQGLRQLLATTPDIVVVGEAGSASEAFELVPQLAPDVVLMDIA
CCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEHH
MPGLDGIAATAALRQRFPTLRVVMLTMYEPTTSGEAARAAGAIAYVVKSSRPEELFAAIR
CCCCCHHHHHHHHHHHCCHHEEEEEEEECCCCCCCHHHHHHHHHEEHHCCCHHHHHHHHH
AAANGAIPPNGEVRAGQSPPPPGRDTADRVAQLEQRLAWLEAQLAQLGVREPLPPSHAPA
HHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
EPVMSAPPADQPIAPSPPVGAVSRHPVWQPSIGTARAADTMPTPRSVSPQPLPVALTQLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
NSLLLGILVVGELVALASALLLFPSPILARWITLGAGQLASLALITLVALAPQRRSEGQI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCE
VLLVALTAAMAWSLLTARSAPVALQAAALAGTSSLALAALALSWQRRFLLAAALSLALIT
EEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLPSLLAIIPGALAPLWVTGTVALASGLAWSRLRRPSDPVAWEWLPLAPLALGTPLLLVW
HHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCEEEECCCHHHHCCCEEEEE
LESGGRPALLLAAPWIAAFPPLVAAGKAQRPVLRALLALHGALALGTVGWLFRTAAHSEQ
ECCCCCCEEEEECCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AAALAVLAVLASALALLLWWSGQRAGASTAPAVVPAAFAGLSLALAAARHPEPVLAPLVW
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
CTLGLVLAALPSRCTWSTWIALALFAGGCGAALLAVLRSPDWQRWSLLVLVLGTATMTGP
HHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
FLAPRWWFVTGWLGSGVLALAALLALRLTGLVEIAALSGLALGTLVVTRYIVPRLDHPTG
CCCCCHHEEECHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AWVWLPTLGASASALASALTGPLSPGRLGIALQPAVSATSEPVIAASMLVAAALAAGRLL
CEEEECCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
GRRWRWAAIAIALLLIAYVLPAVVPDAALVLSWSALAVALAHALGIRLRP
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PERAIRILLVDDHALFRQGLRQLLATTPDIVVVGEAGSASEAFELVPQLAPDVVLMDIA
CCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEHH
MPGLDGIAATAALRQRFPTLRVVMLTMYEPTTSGEAARAAGAIAYVVKSSRPEELFAAIR
CCCCCHHHHHHHHHHHCCHHEEEEEEEECCCCCCCHHHHHHHHHEEHHCCCHHHHHHHHH
AAANGAIPPNGEVRAGQSPPPPGRDTADRVAQLEQRLAWLEAQLAQLGVREPLPPSHAPA
HHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
EPVMSAPPADQPIAPSPPVGAVSRHPVWQPSIGTARAADTMPTPRSVSPQPLPVALTQLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
NSLLLGILVVGELVALASALLLFPSPILARWITLGAGQLASLALITLVALAPQRRSEGQI
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCE
VLLVALTAAMAWSLLTARSAPVALQAAALAGTSSLALAALALSWQRRFLLAAALSLALIT
EEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLPSLLAIIPGALAPLWVTGTVALASGLAWSRLRRPSDPVAWEWLPLAPLALGTPLLLVW
HHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCEEEECCCHHHHCCCEEEEE
LESGGRPALLLAAPWIAAFPPLVAAGKAQRPVLRALLALHGALALGTVGWLFRTAAHSEQ
ECCCCCCEEEEECCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AAALAVLAVLASALALLLWWSGQRAGASTAPAVVPAAFAGLSLALAAARHPEPVLAPLVW
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
CTLGLVLAALPSRCTWSTWIALALFAGGCGAALLAVLRSPDWQRWSLLVLVLGTATMTGP
HHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
FLAPRWWFVTGWLGSGVLALAALLALRLTGLVEIAALSGLALGTLVVTRYIVPRLDHPTG
CCCCCHHEEECHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AWVWLPTLGASASALASALTGPLSPGRLGIALQPAVSATSEPVIAASMLVAAALAAGRLL
CEEEECCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
GRRWRWAAIAIALLLIAYVLPAVVPDAALVLSWSALAVALAHALGIRLRP
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]