Click here to switch to the map view.

The map label for this gene is pimA [H]

Identifier: 221633937

GI number: 221633937

Start: 1983329

End: 1984516

Strand: Reverse

Name: pimA [H]

Synonym: trd_1965

Alternate gene names: 221633937

Gene position: 1984516-1983329 (Counterclockwise)

Preceding gene: 221633939

Following gene: 221633936

Centisome position: 99.08

GC content: 62.79

Gene sequence:

>1188_bases
ATGCGGATCCTCTTCGTCTCCCCGTTCGATTTCAGCTGTCCTGGTGGCGTCAACGAGCACATTCTCCATCTCGATGCAGA
ATATCGCAAGCTTGGCCACCAGACCCGCATCCTGGCTCCGCGCTCGCCCGGCGAAGCAGAAAGCGATGACGGTCACATCG
TCAAGCTCGGCGTCGCCGTACCGATCCCCGCCAACGGATCGACAGCACGGATCACGCTCTCGCCGTTCGTTTCCGGTAAG
GTGAAGGATCTCCTGCGCCGCGAACGGTTCGACATCGTCCATCTCCACGAGCCGCTGGCCCCGATGTTACCGCAGCTGGC
GCTGCTCCACTCCCGGTCGGTCAATGTCGGCACGTTTCACGCTGCCCGAGCCCACAACTGGGGCTACCTTTCGGCTCGTC
CCCTTTTGAGCCTCCTCTTCGCCAAACTGCACGGACGCATCGCCGTTTCACCAGCAGCGTTGGAATCGATCGCGCGCTAT
TTCCCCGGTTATTACGAACTCATCCCCAACGGGATCGATGTTCAGCGCTTCCGGCCCGACGTTCCCCCAATCGCCCAGTT
CGGCCGACCGACCCTGCTCTTCGTCGGTCGCTTCAACGAGTCCCGGAAAGGGTTGCGCTATCTCATCGAAGCCCTGGCAC
TCGTCCGTCGCGAATTTCCCGACGTGCTCTTGCTGGTCGTCGGTCCCGGTGATCCCGCACGCTATGCTCGGCAGCTGGAA
CGCCACGAACTCGTCGATCATGTTCGCTTCACCGGCCCTGTGGCGGATGAGGTGTTACCCGCATACTACACAGCGTGCGA
CGTGTTCTGTGCCCCAGCCACGGGCGGCGAAAGCTTTGGGATCGTCCTGCTCGAAGCGATGGCCAGCGGCAAGCCGGTCG
TGGCCACCGATATCCGCGGCTTTCGCTTCGTCCTGCGTCACGGTATCGAGGGGTTGCTCGTCGAACGGAAGAATCCCGAG
GTGCTCGCCCTCGCGCTCGTGCACCTTCTCGCAGACCCGGCACTGCGCGAGCGATTGGGAAAGGCCGGACGGCAGCGAGC
CGAGCAGTTCTCCTGGGCAGCGATCGCCCAGCGGACACTCGCGTACTACGAGCGACTCTTGCGCGGTGTTCGCGAGACGA
CAACCGAACCACCGATCGATTGGTGGACCTCGCTCCGTCTCGCCTTGAAGGCATGGTCAGGGAGGTAA

Upstream 100 bases:

>100_bases
GTCGCCAGATCGTCGCCAGCATCCACCACAGTCCACTCGCGAAGAGGACAATGGACCAACTGGTCATGGTCTCCTCTCTC
GAATGGTGACGGAGCGTGCA

Downstream 100 bases:

>100_bases
GCTGCAGTGCTCAGTTCACTGGTAGCCGACCGGGTCCGTCTCCGCTTGCAGCGAGTCGGCGAACTCGTCGGGCGAACTGG
CCTCACACCCAACCAGTTGA

Product: glycosyl transferase

Products: NA

Alternate protein names: Alpha-mannosyltransferase; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRILFVSPFDFSCPGGVNEHILHLDAEYRKLGHQTRILAPRSPGEAESDDGHIVKLGVAVPIPANGSTARITLSPFVSGK
VKDLLRRERFDIVHLHEPLAPMLPQLALLHSRSVNVGTFHAARAHNWGYLSARPLLSLLFAKLHGRIAVSPAALESIARY
FPGYYELIPNGIDVQRFRPDVPPIAQFGRPTLLFVGRFNESRKGLRYLIEALALVRREFPDVLLLVVGPGDPARYARQLE
RHELVDHVRFTGPVADEVLPAYYTACDVFCAPATGGESFGIVLLEAMASGKPVVATDIRGFRFVLRHGIEGLLVERKNPE
VLALALVHLLADPALRERLGKAGRQRAEQFSWAAIAQRTLAYYERLLRGVRETTTEPPIDWWTSLRLALKAWSGR

Sequences:

>Translated_395_residues
MRILFVSPFDFSCPGGVNEHILHLDAEYRKLGHQTRILAPRSPGEAESDDGHIVKLGVAVPIPANGSTARITLSPFVSGK
VKDLLRRERFDIVHLHEPLAPMLPQLALLHSRSVNVGTFHAARAHNWGYLSARPLLSLLFAKLHGRIAVSPAALESIARY
FPGYYELIPNGIDVQRFRPDVPPIAQFGRPTLLFVGRFNESRKGLRYLIEALALVRREFPDVLLLVVGPGDPARYARQLE
RHELVDHVRFTGPVADEVLPAYYTACDVFCAPATGGESFGIVLLEAMASGKPVVATDIRGFRFVLRHGIEGLLVERKNPE
VLALALVHLLADPALRERLGKAGRQRAEQFSWAAIAQRTLAYYERLLRGVRETTTEPPIDWWTSLRLALKAWSGR
>Mature_395_residues
MRILFVSPFDFSCPGGVNEHILHLDAEYRKLGHQTRILAPRSPGEAESDDGHIVKLGVAVPIPANGSTARITLSPFVSGK
VKDLLRRERFDIVHLHEPLAPMLPQLALLHSRSVNVGTFHAARAHNWGYLSARPLLSLLFAKLHGRIAVSPAALESIARY
FPGYYELIPNGIDVQRFRPDVPPIAQFGRPTLLFVGRFNESRKGLRYLIEALALVRREFPDVLLLVVGPGDPARYARQLE
RHELVDHVRFTGPVADEVLPAYYTACDVFCAPATGGESFGIVLLEAMASGKPVVATDIRGFRFVLRHGIEGLLVERKNPE
VLALALVHLLADPALRERLGKAGRQRAEQFSWAAIAQRTLAYYERLLRGVRETTTEPPIDWWTSLRLALKAWSGR

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to the position 2 of a phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) [H]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI9755344, Length=402, Percent_Identity=23.134328358209, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: =2.4.1.57 [H]

Molecular weight: Translated: 43992; Mature: 43992

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILFVSPFDFSCPGGVNEHILHLDAEYRKLGHQTRILAPRSPGEAESDDGHIVKLGVAV
CEEEEECCCCCCCCCCCCCCEEEECHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEEEE
PIPANGSTARITLSPFVSGKVKDLLRRERFDIVHLHEPLAPMLPQLALLHSRSVNVGTFH
ECCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCCCEEE
AARAHNWGYLSARPLLSLLFAKLHGRIAVSPAALESIARYFPGYYELIPNGIDVQRFRPD
HHHCCCCCCCCHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCHHHHCCCCCCHHHCCCC
VPPIAQFGRPTLLFVGRFNESRKGLRYLIEALALVRREFPDVLLLVVGPGDPARYARQLE
CCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
RHELVDHVRFTGPVADEVLPAYYTACDVFCAPATGGESFGIVLLEAMASGKPVVATDIRG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCCEEEECHHH
FRFVLRHGIEGLLVERKNPEVLALALVHLLADPALRERLGKAGRQRAEQFSWAAIAQRTL
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYYERLLRGVRETTTEPPIDWWTSLRLALKAWSGR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRILFVSPFDFSCPGGVNEHILHLDAEYRKLGHQTRILAPRSPGEAESDDGHIVKLGVAV
CEEEEECCCCCCCCCCCCCCEEEECHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEEEE
PIPANGSTARITLSPFVSGKVKDLLRRERFDIVHLHEPLAPMLPQLALLHSRSVNVGTFH
ECCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCCCEEE
AARAHNWGYLSARPLLSLLFAKLHGRIAVSPAALESIARYFPGYYELIPNGIDVQRFRPD
HHHCCCCCCCCHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCHHHHCCCCCCHHHCCCC
VPPIAQFGRPTLLFVGRFNESRKGLRYLIEALALVRREFPDVLLLVVGPGDPARYARQLE
CCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
RHELVDHVRFTGPVADEVLPAYYTACDVFCAPATGGESFGIVLLEAMASGKPVVATDIRG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCCEEEECHHH
FRFVLRHGIEGLLVERKNPEVLALALVHLLADPALRERLGKAGRQRAEQFSWAAIAQRTL
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYYERLLRGVRETTTEPPIDWWTSLRLALKAWSGR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]