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The map label for this gene is rhmD [H]

Identifier: 221633928

GI number: 221633928

Start: 1974154

End: 1975326

Strand: Reverse

Name: rhmD [H]

Synonym: trd_1956

Alternate gene names: 221633928

Gene position: 1975326-1974154 (Counterclockwise)

Preceding gene: 221633930

Following gene: 221633925

Centisome position: 98.62

GC content: 59.59

Gene sequence:

>1173_bases
ATGCGTATCGTAGACATAAAAACTGCTGTTATTGCCGGTAATTTCGACTGGATTCTGGTTCGTGTCGAGACCGACGAGGG
ATTGAGCGGGCTCGGTGAGGCATATTGGGGAACTGGGGTTGTGGAACTCATCGATGCAGCCAAGCGCCTGATCGTCGGAG
AAAATCCGTTCGATGTATCGAAACTACTTTTTAAAATGCAGCGCGGACTCTCTGGAGCCGGCGCACAAGCCGGAGCGACC
GTTACGGCGATCAGTGGCATCGAAATAGCCCTTTGGGATCTGGTAGCACGAGCATTGAACACTCCGCTTTCGACCCTTTT
TGGAGGTCGGTTCCGTTCACAAATTCGCGTCTATGCTGATCTCCATGCTGGGGAAGAACCCACTCCGGAGAGTTGGGCTC
GTCGCGCGAAGCAGGCAGTAGAGCAGGGTTTTACTGCCATCAAGTTCGACCTCGATGCACCGAACCCCTATACCCGCGAC
ATCTCCGACGATGCCGATCCTCGCCGTTCCTGGTACGAGCCATACAATCGAACAGTCGGCAGCGCCGAGCGCCGCTACCT
CCAGCAGGTGGTCGCCGCAGTGCGCGAGACGATCGGGCCTGATATCATGCTTGCCCTCGACTGCCATTGGAAGTACGCTG
TCCCGGACGTGATCCAGCTTGCGCATGCCTTGGAGCCGTACGATCCGCTCTGGCTGGAAGATCCGATCCCGCCAGACAAT
GTCGATGCGTTGGTGACCGTGGCCCGCAGCACGCGCATCCCGATCTGTACCGGCGAGAACCACTACCGGCTGCAGGGATT
CCGCGAGCTCATCGAGCGACAGGCCTGTAGCATTGTGGCTCCCGACATCCCGAAGATGGGTGGCCTCCTCGAGGCGCGCA
CAGTCGCAGCATGGGCGGACCTCTATGGGATGCTCGTGGCTCCGCACAACGTCTGCAGCCCGATCGGCACGGTGGCCGCA
GCGCACCTCTGTGCAGCGATCTCCAACTTCCTCGTGTTGGAATTTCACGCGCTCGATGTGCCATGGTGGGATGAAATCGT
CCTCGAGCGACCGGTCATTCGCAACGGGTTCATCACCCTCAGCGACGCACCAGGCCACGGCCTCACGCTCAATGAGGAGG
TTGCGCGCGCCCACGCCAAGCCGGGAACGACCTTCTTCGGTGAACCCGTGTGA

Upstream 100 bases:

>100_bases
ATTGAACCCCATGGCTGCTCCCCTCGCCGATTGTGCCTGCTCATTTGACAATGTTACTATCTCATATTGAGGCCATCAAC
CATATGAAAGGAGTCCAGAG

Downstream 100 bases:

>100_bases
GCGATCGTCGCACTCAGTTCGGCGCTTCTGCGCCTGTTCCTGCGCGGTTGCGCAAGACTACGGTGAGGTCATCGTTCGTA
ACGACCGGCTGCTCCCTATA

Product: 2-oxo-3-deoxygalactonate 6-phosphate aldolase and galactonate dehydratase

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MRIVDIKTAVIAGNFDWILVRVETDEGLSGLGEAYWGTGVVELIDAAKRLIVGENPFDVSKLLFKMQRGLSGAGAQAGAT
VTAISGIEIALWDLVARALNTPLSTLFGGRFRSQIRVYADLHAGEEPTPESWARRAKQAVEQGFTAIKFDLDAPNPYTRD
ISDDADPRRSWYEPYNRTVGSAERRYLQQVVAAVRETIGPDIMLALDCHWKYAVPDVIQLAHALEPYDPLWLEDPIPPDN
VDALVTVARSTRIPICTGENHYRLQGFRELIERQACSIVAPDIPKMGGLLEARTVAAWADLYGMLVAPHNVCSPIGTVAA
AHLCAAISNFLVLEFHALDVPWWDEIVLERPVIRNGFITLSDAPGHGLTLNEEVARAHAKPGTTFFGEPV

Sequences:

>Translated_390_residues
MRIVDIKTAVIAGNFDWILVRVETDEGLSGLGEAYWGTGVVELIDAAKRLIVGENPFDVSKLLFKMQRGLSGAGAQAGAT
VTAISGIEIALWDLVARALNTPLSTLFGGRFRSQIRVYADLHAGEEPTPESWARRAKQAVEQGFTAIKFDLDAPNPYTRD
ISDDADPRRSWYEPYNRTVGSAERRYLQQVVAAVRETIGPDIMLALDCHWKYAVPDVIQLAHALEPYDPLWLEDPIPPDN
VDALVTVARSTRIPICTGENHYRLQGFRELIERQACSIVAPDIPKMGGLLEARTVAAWADLYGMLVAPHNVCSPIGTVAA
AHLCAAISNFLVLEFHALDVPWWDEIVLERPVIRNGFITLSDAPGHGLTLNEEVARAHAKPGTTFFGEPV
>Mature_390_residues
MRIVDIKTAVIAGNFDWILVRVETDEGLSGLGEAYWGTGVVELIDAAKRLIVGENPFDVSKLLFKMQRGLSGAGAQAGAT
VTAISGIEIALWDLVARALNTPLSTLFGGRFRSQIRVYADLHAGEEPTPESWARRAKQAVEQGFTAIKFDLDAPNPYTRD
ISDDADPRRSWYEPYNRTVGSAERRYLQQVVAAVRETIGPDIMLALDCHWKYAVPDVIQLAHALEPYDPLWLEDPIPPDN
VDALVTVARSTRIPICTGENHYRLQGFRELIERQACSIVAPDIPKMGGLLEARTVAAWADLYGMLVAPHNVCSPIGTVAA
AHLCAAISNFLVLEFHALDVPWWDEIVLERPVIRNGFITLSDAPGHGLTLNEEVARAHAKPGTTFFGEPV

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=407, Percent_Identity=20.3931203931204, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI186972148, Length=347, Percent_Identity=21.0374639769452, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI48994953, Length=344, Percent_Identity=28.7790697674419, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787864, Length=394, Percent_Identity=25.6345177664975, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI226510960, Length=368, Percent_Identity=28.2608695652174, Blast_Score=118, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 42841; Mature: 42841

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVDIKTAVIAGNFDWILVRVETDEGLSGLGEAYWGTGVVELIDAAKRLIVGENPFDVS
CEEEEEEEEEEECCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHEECCCCCHHH
KLLFKMQRGLSGAGAQAGATVTAISGIEIALWDLVARALNTPLSTLFGGRFRSQIRVYAD
HHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHCHHHHHHEEEEEE
LHAGEEPTPESWARRAKQAVEQGFTAIKFDLDAPNPYTRDISDDADPRRSWYEPYNRTVG
ECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCHHHCCCC
SAERRYLQQVVAAVRETIGPDIMLALDCHWKYAVPDVIQLAHALEPYDPLWLEDPIPPDN
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
VDALVTVARSTRIPICTGENHYRLQGFRELIERQACSIVAPDIPKMGGLLEARTVAAWAD
HHHHHEEHHCCCCCEEECCCCHHHHHHHHHHHHHHCCEECCCCCHHCCHHHHHHHHHHHH
LYGMLVAPHNVCSPIGTVAAAHLCAAISNFLVLEFHALDVPWWDEIVLERPVIRNGFITL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHCCCCHHCCCEEE
SDAPGHGLTLNEEVARAHAKPGTTFFGEPV
ECCCCCCCEECHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRIVDIKTAVIAGNFDWILVRVETDEGLSGLGEAYWGTGVVELIDAAKRLIVGENPFDVS
CEEEEEEEEEEECCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHEECCCCCHHH
KLLFKMQRGLSGAGAQAGATVTAISGIEIALWDLVARALNTPLSTLFGGRFRSQIRVYAD
HHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHCHHHHHHEEEEEE
LHAGEEPTPESWARRAKQAVEQGFTAIKFDLDAPNPYTRDISDDADPRRSWYEPYNRTVG
ECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCHHHCCCC
SAERRYLQQVVAAVRETIGPDIMLALDCHWKYAVPDVIQLAHALEPYDPLWLEDPIPPDN
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
VDALVTVARSTRIPICTGENHYRLQGFRELIERQACSIVAPDIPKMGGLLEARTVAAWAD
HHHHHEEHHCCCCCEEECCCCHHHHHHHHHHHHHHCCEECCCCCHHCCHHHHHHHHHHHH
LYGMLVAPHNVCSPIGTVAAAHLCAAISNFLVLEFHALDVPWWDEIVLERPVIRNGFITL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHCCCCHHCCCEEE
SDAPGHGLTLNEEVARAHAKPGTTFFGEPV
ECCCCCCCEECHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA