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The map label for this gene is mrdA [H]

Identifier: 221633900

GI number: 221633900

Start: 1936420

End: 1938696

Strand: Reverse

Name: mrdA [H]

Synonym: trd_1927

Alternate gene names: 221633900

Gene position: 1938696-1936420 (Counterclockwise)

Preceding gene: 221633901

Following gene: 221633899

Centisome position: 96.79

GC content: 68.29

Gene sequence:

>2277_bases
GTGCGCACTAGGCCGGCCCGGCGCGGACTCGATCTTCCCGCGCCGAAGTCGATCGCGCCACCAGAAGAACCGGAACCGAC
GCGCGACGAGCGGATGACGCGACGGGTGTTCCTGCTGCGCGGCGCGATGGTGCTCGGCTTCGGCGCAGTCCTGGCCAAGC
TCGGCATCATGCAGCTGCGCGAGCGCCCACGCTACCAGGCAGCCGCTCAGGGCAGCATGGTGCGCTTCGAACCCTTGCGC
GCCCCGCGCGGACTGATCGTCGATCGCAACGGCACCGTGCTCGCCCGGAACCGACGGAGCTGGACCGTGTGGCTGGTCCC
CGCCGAACTCCCCGACGACGAGGCAACGTACCGGCAGGTGCGGGACCAGATCGTCGCCGCTCTGGGACTGGACGATGTCC
TAGTCGTGCACCGGCCAGCGCTGCCACCCGGCGAGGAGCGGGCGGTTCTCGACGCACTCGGCACGGTGCTCGGTGTGCCC
GGAGCTGACCTGGCAGGGCTGGTTTTTTCCGCCCCCAAGGACCGCAAGTACGTCATCGTGCGCGACAAGCTCCGACCGGA
AGAAGCCGCAGCCCTGCGCCAGGAAGCCAAGCGCCTCCCAGGGGTGCATGTCCTCAACCGGTTCGATTACCTGGCGCAGT
CGGCAGCGGGGAGCGCCGTACCGGTCGTGCTCGCCGACGACATCCCGCGCGAACTCGCACTCACGCTGGCAGCGAACCCG
GCTGCTCTCCCCGGGATCCACGTGAGCGACGACAAGCTCGTCCGCGAGTACCCGGCGGGGCCGGAGTTCGCGCACATCCT
GGGCTACGTCGGGCCAATCACCAAGGAAGAATACGACGCAGCGGGCGGCGACAGTCCGGCCAATCCGTATCGTCCCGATG
ACCGCGTCGGGCGTGGCGGAGTCGAGCAAGCGATGGAACCCGCCCTGCGCGGTCAGCACGGGGGACGCTGGGTGCAGGTC
GATGCCCGCAACGTGATCGTCGGTGAACTGGTCGAGCGACGGCGCGAGCCGGTCCCGGGACTCACCGTGGTGCTGACCAT
CGACTCGACCCTGCAAAAGGCCGTCACCGCGGCGCTGGCCGAAGGGATCCGCGCTGCCGATCGCGCTGCCCGCGAAAAAG
GACGCGATCCGGTCGGGTCCGGCGTGGCCATCGTGCTCGATCCGCGCTCGGGCGAGGTCCTGGCACTGGTCTCCCTGCCG
ACCTATGACAATCAAAAGTTCGTGCATGGCCTGAGCCAGGCAGAATACCAGGCGCTCCTCGACGACCCCTTCCACCCGCT
GGTCAACTATGCGATCAGCGGCGAGTTCCCGCCCGGCTCCACCGTCAAGCCGCTTCTCGCCTGCGCGGGGCTGCAGGAAG
GGGTCATCACGCTGGCGAGCACCTTCACCTGCCGGGGAGCGATCCGCGTGCCGACTGTCTGGGACGAGGCAGGCGGCAAC
ACCTACGTCTGCTGGCTGGCCAGCGGGCACGGTGAGGTCGACGTCCTCGACGGGATCGCCCAATCCTGCAACGTGTTCTT
CTACAACGTCGGGGCGCCCGGCCAAACGCCGGAAGGTGGCAGCGAGCCGCTCCATTACTACATCCCCGGTGACCCGGCCA
AGCACTACTTCGTCGGACTGGGGATCGAGCGGATCGAGCAGTACTTGCGCCAGGTGTTCGGCTTCGGGCAACCGACCGGT
ATCGAGCTCGCCGGCGAAGCCGACGGCCTGGTGCCGAACCCGCGCTGGCTGTTCCAGACGTTGCGGGAGTACTGGTCGGT
CGGAGATACTATAAACGTCTCCATCGGCCAGGGACACCTGGCCTGCACGCCGTTGCAGCTGGCCTGTGCCTTGGCTGCCA
TCGCCAATGGGGGGACCTACTATCGGCCGCGGATCGTCCGCGAACTGCGCGACGCGAGTGGGCAGGTGGTCCGGCGGTTC
GAGCCGGAAGCGGCGCGTCAGCTGGCGATCGCGCCCGAGCATCTCGAGACGGTCCGCCGCGGCATGCGCCGCACCGTCAC
CGATGGGACGGCGAAAGGAAAGTTCGTGCTGACGGGAGACGACATCCCGATCGCCGGCAAGACGGGGACAGCCGAGTACG
GCGAGGCGGAGAACGGGCGCTACAAGAAGGCGCATGCCTGGTTCACCGCCTTCGCTCCCTACGACGACCCAGCGGTGCTG
GTGCTCGTCCTGATCCGCGATGGGGGAGAGGGCGCGACCTTCGCTGTCCCGGTCGCTGACCGGATCCTGGCGACCTACTT
CGGACGGATCAGCCCGGAAACAGGAGCGAGAGGGTAG

Upstream 100 bases:

>100_bases
GACGCGGGAGTGACCGCCATGCTGGTCGGCATCCTCAACCTGATCGCCGTCCCGCTGGTCTACGGCATCGTCCTACTCGC
TGGACGTCTGGGGGTCAGGC

Downstream 100 bases:

>100_bases
GCGATGCTCGACGACGAACGAGCACGAGCGACGGTCCGCGTGCGTGGAACCCAGGACGGCGTCCGCCTCCTGTTGCCGCG
CGCCACCCCCCTCGCGGTGG

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MRTRPARRGLDLPAPKSIAPPEEPEPTRDERMTRRVFLLRGAMVLGFGAVLAKLGIMQLRERPRYQAAAQGSMVRFEPLR
APRGLIVDRNGTVLARNRRSWTVWLVPAELPDDEATYRQVRDQIVAALGLDDVLVVHRPALPPGEERAVLDALGTVLGVP
GADLAGLVFSAPKDRKYVIVRDKLRPEEAAALRQEAKRLPGVHVLNRFDYLAQSAAGSAVPVVLADDIPRELALTLAANP
AALPGIHVSDDKLVREYPAGPEFAHILGYVGPITKEEYDAAGGDSPANPYRPDDRVGRGGVEQAMEPALRGQHGGRWVQV
DARNVIVGELVERRREPVPGLTVVLTIDSTLQKAVTAALAEGIRAADRAAREKGRDPVGSGVAIVLDPRSGEVLALVSLP
TYDNQKFVHGLSQAEYQALLDDPFHPLVNYAISGEFPPGSTVKPLLACAGLQEGVITLASTFTCRGAIRVPTVWDEAGGN
TYVCWLASGHGEVDVLDGIAQSCNVFFYNVGAPGQTPEGGSEPLHYYIPGDPAKHYFVGLGIERIEQYLRQVFGFGQPTG
IELAGEADGLVPNPRWLFQTLREYWSVGDTINVSIGQGHLACTPLQLACALAAIANGGTYYRPRIVRELRDASGQVVRRF
EPEAARQLAIAPEHLETVRRGMRRTVTDGTAKGKFVLTGDDIPIAGKTGTAEYGEAENGRYKKAHAWFTAFAPYDDPAVL
VLVLIRDGGEGATFAVPVADRILATYFGRISPETGARG

Sequences:

>Translated_758_residues
MRTRPARRGLDLPAPKSIAPPEEPEPTRDERMTRRVFLLRGAMVLGFGAVLAKLGIMQLRERPRYQAAAQGSMVRFEPLR
APRGLIVDRNGTVLARNRRSWTVWLVPAELPDDEATYRQVRDQIVAALGLDDVLVVHRPALPPGEERAVLDALGTVLGVP
GADLAGLVFSAPKDRKYVIVRDKLRPEEAAALRQEAKRLPGVHVLNRFDYLAQSAAGSAVPVVLADDIPRELALTLAANP
AALPGIHVSDDKLVREYPAGPEFAHILGYVGPITKEEYDAAGGDSPANPYRPDDRVGRGGVEQAMEPALRGQHGGRWVQV
DARNVIVGELVERRREPVPGLTVVLTIDSTLQKAVTAALAEGIRAADRAAREKGRDPVGSGVAIVLDPRSGEVLALVSLP
TYDNQKFVHGLSQAEYQALLDDPFHPLVNYAISGEFPPGSTVKPLLACAGLQEGVITLASTFTCRGAIRVPTVWDEAGGN
TYVCWLASGHGEVDVLDGIAQSCNVFFYNVGAPGQTPEGGSEPLHYYIPGDPAKHYFVGLGIERIEQYLRQVFGFGQPTG
IELAGEADGLVPNPRWLFQTLREYWSVGDTINVSIGQGHLACTPLQLACALAAIANGGTYYRPRIVRELRDASGQVVRRF
EPEAARQLAIAPEHLETVRRGMRRTVTDGTAKGKFVLTGDDIPIAGKTGTAEYGEAENGRYKKAHAWFTAFAPYDDPAVL
VLVLIRDGGEGATFAVPVADRILATYFGRISPETGARG
>Mature_758_residues
MRTRPARRGLDLPAPKSIAPPEEPEPTRDERMTRRVFLLRGAMVLGFGAVLAKLGIMQLRERPRYQAAAQGSMVRFEPLR
APRGLIVDRNGTVLARNRRSWTVWLVPAELPDDEATYRQVRDQIVAALGLDDVLVVHRPALPPGEERAVLDALGTVLGVP
GADLAGLVFSAPKDRKYVIVRDKLRPEEAAALRQEAKRLPGVHVLNRFDYLAQSAAGSAVPVVLADDIPRELALTLAANP
AALPGIHVSDDKLVREYPAGPEFAHILGYVGPITKEEYDAAGGDSPANPYRPDDRVGRGGVEQAMEPALRGQHGGRWVQV
DARNVIVGELVERRREPVPGLTVVLTIDSTLQKAVTAALAEGIRAADRAAREKGRDPVGSGVAIVLDPRSGEVLALVSLP
TYDNQKFVHGLSQAEYQALLDDPFHPLVNYAISGEFPPGSTVKPLLACAGLQEGVITLASTFTCRGAIRVPTVWDEAGGN
TYVCWLASGHGEVDVLDGIAQSCNVFFYNVGAPGQTPEGGSEPLHYYIPGDPAKHYFVGLGIERIEQYLRQVFGFGQPTG
IELAGEADGLVPNPRWLFQTLREYWSVGDTINVSIGQGHLACTPLQLACALAAIANGGTYYRPRIVRELRDASGQVVRRF
EPEAARQLAIAPEHLETVRRGMRRTVTDGTAKGKFVLTGDDIPIAGKTGTAEYGEAENGRYKKAHAWFTAFAPYDDPAVL
VLVLIRDGGEGATFAVPVADRILATYFGRISPETGARG

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=728, Percent_Identity=28.7087912087912, Blast_Score=250, Evalue=3e-67,
Organism=Escherichia coli, GI1786272, Length=743, Percent_Identity=23.6877523553163, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 81889; Mature: 81889

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRPARRGLDLPAPKSIAPPEEPEPTRDERMTRRVFLLRGAMVLGFGAVLAKLGIMQLR
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERPRYQAAAQGSMVRFEPLRAPRGLIVDRNGTVLARNRRSWTVWLVPAELPDDEATYRQV
HCCCHHHHHCCCEEEEECCCCCCCEEEECCCCEEEECCCEEEEEEEECCCCCCHHHHHHH
RDQIVAALGLDDVLVVHRPALPPGEERAVLDALGTVLGVPGADLAGLVFSAPKDRKYVIV
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEE
RDKLRPEEAAALRQEAKRLPGVHVLNRFDYLAQSAAGSAVPVVLADDIPRELALTLAANP
ECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHEEEEECCC
AALPGIHVSDDKLVREYPAGPEFAHILGYVGPITKEEYDAAGGDSPANPYRPDDRVGRGG
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCC
VEQAMEPALRGQHGGRWVQVDARNVIVGELVERRREPVPGLTVVLTIDSTLQKAVTAALA
HHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHH
EGIRAADRAAREKGRDPVGSGVAIVLDPRSGEVLALVSLPTYDNQKFVHGLSQAEYQALL
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
DDPFHPLVNYAISGEFPPGSTVKPLLACAGLQEGVITLASTFTCRGAIRVPTVWDEAGGN
CCCCHHHHHHEECCCCCCCCCHHHHHHHCCCHHCHHHHHHHHEECCEEECCEEEECCCCC
TYVCWLASGHGEVDVLDGIAQSCNVFFYNVGAPGQTPEGGSEPLHYYIPGDPAKHYFVGL
EEEEEEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEC
GIERIEQYLRQVFGFGQPTGIELAGEADGLVPNPRWLFQTLREYWSVGDTINVSIGQGHL
CHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCE
ACTPLQLACALAAIANGGTYYRPRIVRELRDASGQVVRRFEPEAARQLAIAPEHLETVRR
EECHHHHHHHHHHHHCCCCEECHHHHHHHHHCCCHHHHHCCHHHHHHHCCCHHHHHHHHH
GMRRTVTDGTAKGKFVLTGDDIPIAGKTGTAEYGEAENGRYKKAHAWFTAFAPYDDPAVL
HHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEE
VLVLIRDGGEGATFAVPVADRILATYFGRISPETGARG
EEEEEEECCCCCEEEECHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRTRPARRGLDLPAPKSIAPPEEPEPTRDERMTRRVFLLRGAMVLGFGAVLAKLGIMQLR
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERPRYQAAAQGSMVRFEPLRAPRGLIVDRNGTVLARNRRSWTVWLVPAELPDDEATYRQV
HCCCHHHHHCCCEEEEECCCCCCCEEEECCCCEEEECCCEEEEEEEECCCCCCHHHHHHH
RDQIVAALGLDDVLVVHRPALPPGEERAVLDALGTVLGVPGADLAGLVFSAPKDRKYVIV
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEE
RDKLRPEEAAALRQEAKRLPGVHVLNRFDYLAQSAAGSAVPVVLADDIPRELALTLAANP
ECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHEEEEECCC
AALPGIHVSDDKLVREYPAGPEFAHILGYVGPITKEEYDAAGGDSPANPYRPDDRVGRGG
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCC
VEQAMEPALRGQHGGRWVQVDARNVIVGELVERRREPVPGLTVVLTIDSTLQKAVTAALA
HHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHH
EGIRAADRAAREKGRDPVGSGVAIVLDPRSGEVLALVSLPTYDNQKFVHGLSQAEYQALL
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
DDPFHPLVNYAISGEFPPGSTVKPLLACAGLQEGVITLASTFTCRGAIRVPTVWDEAGGN
CCCCHHHHHHEECCCCCCCCCHHHHHHHCCCHHCHHHHHHHHEECCEEECCEEEECCCCC
TYVCWLASGHGEVDVLDGIAQSCNVFFYNVGAPGQTPEGGSEPLHYYIPGDPAKHYFVGL
EEEEEEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEC
GIERIEQYLRQVFGFGQPTGIELAGEADGLVPNPRWLFQTLREYWSVGDTINVSIGQGHL
CHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCE
ACTPLQLACALAAIANGGTYYRPRIVRELRDASGQVVRRFEPEAARQLAIAPEHLETVRR
EECHHHHHHHHHHHHCCCCEECHHHHHHHHHCCCHHHHHCCHHHHHHHCCCHHHHHHHHH
GMRRTVTDGTAKGKFVLTGDDIPIAGKTGTAEYGEAENGRYKKAHAWFTAFAPYDDPAVL
HHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEE
VLVLIRDGGEGATFAVPVADRILATYFGRISPETGARG
EEEEEEECCCCCEEEECHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]