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The map label for this gene is hyaB [C]

Identifier: 221633850

GI number: 221633850

Start: 1885556

End: 1887322

Strand: Reverse

Name: hyaB [C]

Synonym: trd_1877

Alternate gene names: 221633850

Gene position: 1887322-1885556 (Counterclockwise)

Preceding gene: 221633851

Following gene: 221633849

Centisome position: 94.22

GC content: 61.57

Gene sequence:

>1767_bases
ATGGCAGAGGCAGGCACGACCCGAGAACTCGTCGAGATGTCCTGGGACCCGATCACCCGCATCGTGGGCAGTCTCGGGAT
CTACACGAAGATCGACTTCAAGCAGCGCCGCGTGGTCGAGTGCTATAGCACCTCCTCCATCTTCCGCGGCTACAGCGTCT
TCATGCGGGGGAAGGACCCGCGCGACGCTCACTTCATTACGAGCCGGATCTGTGGGATCTGTGGCGATAACCATGCGACC
TGCTCGGTCTATAACCAGAACATGGCCTATGGTGTCGCGCCACCACCGCTCGGGGAGTGGATCATCAATCTCGGCGAAGC
CGCAGAGTATATGTTCGACCACAATATCTTCCAGGAAAACCTGGTCGGGGTCGATTTCTGCGAGCGGATGGTGCGGGAGA
CCAATCCGAGCGTCTGGGAGAAGGCACAGCAAACCGCGGCACCCCACGCACATCTTCACGGCTACCGCACCATCGCCGAC
ATCATGCGCGCGCTAAATCCGCTCGAAGGCGAGTTCTACCGCCAGGCCCTGCAGATGAGCCGGATGACGCGCGAGATGTT
CTGTCTCATGGAAGGCCGCCATGTCCATCCCTCGACGCTCTACCCCGGCGGTGTGGGGACGGTGCCGACAGTCCAACTCT
TCACGGATTACCTGACCCGCTTGATGCGCTACATCGAGTTCATGAAGAAGGCCGTTCCGTTGCATGACGACCTCTTCGAC
TTCTTCTACCAGGCCCTGCCTGGGTACGAAGAGGTCGGCCGGCGCCGCATCCTGCTCGGTTGCTGGGGAGCCTTCAACGA
CCCGGAGTACTGTGACTTCAAGTACGAGCATATGACCGAGTGGGGCCGCCGCATGTTCGTCACCCCCGGAGTGATCGTCG
ATGGCAAGCTGGTGACAAACGATCTCGTGCAGATCAACCTCGGCATCCGCATCCTCCTCGGGAGCTCGTACTACGAGGAC
TGGGAAGATCAGGAGATGTTCGTCACCCGCGACCCGCTCGGCAACCCGGTGGACCGCCGTCATCCGTGGAACGTCCACAC
GATTCCCAAGCCACAGAAGCGGGACTTCAGCGACAAGTACTCGTGGGTGATGTCACCGCGCTGGTTCGATGGCGAGAACT
ACCTCGCGCTCGATACCGGCGGGGGACCGATCGCCCGCTTGTGGTCCACGGCCCTTTCCGGGCTCGTCGACATCGGCTAC
ATCAAGGCCACCGGCTCCAGTGTCATCATCAACCTGCCGAAGACGGCAACGATGCCGGAGATGACCTTCGAGTGGAAGAT
CCCCCAGTGGAGCAACACGATCGAGCGTGACCGCGCACGCACCTACTTCCAGGCGTACGCCGCGGCAGCAGCGCTCTACT
TCGTCGAGAAGGCACTAGACGAGCTGCGGCGCGGCAACACGCAGGTCTGGACTCCCTTCGAGGTCCCCAAGGAGGCGATC
AGCTGCGGTTGGACCGAGGCGGTGCGCGGAGTTCTCTCCCACCATATGGTGATCCGCGACGGGAAGATCGCGAACTACCA
GCCGTGGCCACCCACGCCCTGGAACGCCAACCCGCGGGATATCTACGGCACCCCCGGCCCCTATGAGGACGCGGTCCAGA
ATACGCCGATCTTCGAGGAGAACGGACCGGACAACTTCAAGGGGATCGACATCATGCGCACGGTGCGCAGCTTCGATCCC
TGCCTCCCCTGCGGCGTGCATATGTACCTGGGCAACGGGAAGGTGATCAAGCAGGTCCATTCGCCGACAATGTTGCCAGA
GATCTAA

Upstream 100 bases:

>100_bases
AAGTGGCGCCGCAAGGGGAGCGAGCTGGCCACCGGGTACGAGAAGACTCGCTATGACTAGGCAGCCGGCACCTCAGGCTT
GACAGGGAAGGAGCGGACCG

Downstream 100 bases:

>100_bases
GAACAGCTCTCCAGCTTGGGAACCGGGAGAGCTGCTCTGCCCACCGGTCGTTGCCTCTCGGGTTGCCTCCCGCAGGGACA
CCCGATGAGAGGAAGCGGGG

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MAEAGTTRELVEMSWDPITRIVGSLGIYTKIDFKQRRVVECYSTSSIFRGYSVFMRGKDPRDAHFITSRICGICGDNHAT
CSVYNQNMAYGVAPPPLGEWIINLGEAAEYMFDHNIFQENLVGVDFCERMVRETNPSVWEKAQQTAAPHAHLHGYRTIAD
IMRALNPLEGEFYRQALQMSRMTREMFCLMEGRHVHPSTLYPGGVGTVPTVQLFTDYLTRLMRYIEFMKKAVPLHDDLFD
FFYQALPGYEEVGRRRILLGCWGAFNDPEYCDFKYEHMTEWGRRMFVTPGVIVDGKLVTNDLVQINLGIRILLGSSYYED
WEDQEMFVTRDPLGNPVDRRHPWNVHTIPKPQKRDFSDKYSWVMSPRWFDGENYLALDTGGGPIARLWSTALSGLVDIGY
IKATGSSVIINLPKTATMPEMTFEWKIPQWSNTIERDRARTYFQAYAAAAALYFVEKALDELRRGNTQVWTPFEVPKEAI
SCGWTEAVRGVLSHHMVIRDGKIANYQPWPPTPWNANPRDIYGTPGPYEDAVQNTPIFEENGPDNFKGIDIMRTVRSFDP
CLPCGVHMYLGNGKVIKQVHSPTMLPEI

Sequences:

>Translated_588_residues
MAEAGTTRELVEMSWDPITRIVGSLGIYTKIDFKQRRVVECYSTSSIFRGYSVFMRGKDPRDAHFITSRICGICGDNHAT
CSVYNQNMAYGVAPPPLGEWIINLGEAAEYMFDHNIFQENLVGVDFCERMVRETNPSVWEKAQQTAAPHAHLHGYRTIAD
IMRALNPLEGEFYRQALQMSRMTREMFCLMEGRHVHPSTLYPGGVGTVPTVQLFTDYLTRLMRYIEFMKKAVPLHDDLFD
FFYQALPGYEEVGRRRILLGCWGAFNDPEYCDFKYEHMTEWGRRMFVTPGVIVDGKLVTNDLVQINLGIRILLGSSYYED
WEDQEMFVTRDPLGNPVDRRHPWNVHTIPKPQKRDFSDKYSWVMSPRWFDGENYLALDTGGGPIARLWSTALSGLVDIGY
IKATGSSVIINLPKTATMPEMTFEWKIPQWSNTIERDRARTYFQAYAAAAALYFVEKALDELRRGNTQVWTPFEVPKEAI
SCGWTEAVRGVLSHHMVIRDGKIANYQPWPPTPWNANPRDIYGTPGPYEDAVQNTPIFEENGPDNFKGIDIMRTVRSFDP
CLPCGVHMYLGNGKVIKQVHSPTMLPEI
>Mature_587_residues
AEAGTTRELVEMSWDPITRIVGSLGIYTKIDFKQRRVVECYSTSSIFRGYSVFMRGKDPRDAHFITSRICGICGDNHATC
SVYNQNMAYGVAPPPLGEWIINLGEAAEYMFDHNIFQENLVGVDFCERMVRETNPSVWEKAQQTAAPHAHLHGYRTIADI
MRALNPLEGEFYRQALQMSRMTREMFCLMEGRHVHPSTLYPGGVGTVPTVQLFTDYLTRLMRYIEFMKKAVPLHDDLFDF
FYQALPGYEEVGRRRILLGCWGAFNDPEYCDFKYEHMTEWGRRMFVTPGVIVDGKLVTNDLVQINLGIRILLGSSYYEDW
EDQEMFVTRDPLGNPVDRRHPWNVHTIPKPQKRDFSDKYSWVMSPRWFDGENYLALDTGGGPIARLWSTALSGLVDIGYI
KATGSSVIINLPKTATMPEMTFEWKIPQWSNTIERDRARTYFQAYAAAAALYFVEKALDELRRGNTQVWTPFEVPKEAIS
CGWTEAVRGVLSHHMVIRDGKIANYQPWPPTPWNANPRDIYGTPGPYEDAVQNTPIFEENGPDNFKGIDIMRTVRSFDPC
LPCGVHMYLGNGKVIKQVHSPTMLPEI

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=628, Percent_Identity=26.4331210191083, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI1789368, Length=617, Percent_Identity=24.6353322528363, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 67324; Mature: 67192

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAGTTRELVEMSWDPITRIVGSLGIYTKIDFKQRRVVECYSTSSIFRGYSVFMRGKDP
CCCCCCCHHHHHCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCEEEEECCCCC
RDAHFITSRICGICGDNHATCSVYNQNMAYGVAPPPLGEWIINLGEAAEYMFDHNIFQEN
CHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVGVDFCERMVRETNPSVWEKAQQTAAPHAHLHGYRTIADIMRALNPLEGEFYRQALQMS
CCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RMTREMFCLMEGRHVHPSTLYPGGVGTVPTVQLFTDYLTRLMRYIEFMKKAVPLHDDLFD
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FFYQALPGYEEVGRRRILLGCWGAFNDPEYCDFKYEHMTEWGRRMFVTPGVIVDGKLVTN
HHHHHCCCHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCEEEECCCEEECCEEEEC
DLVQINLGIRILLGSSYYEDWEDQEMFVTRDPLGNPVDRRHPWNVHTIPKPQKRDFSDKY
CEEEEECCEEEEECCCHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
SWVMSPRWFDGENYLALDTGGGPIARLWSTALSGLVDIGYIKATGSSVIINLPKTATMPE
HEEECCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCC
MTFEWKIPQWSNTIERDRARTYFQAYAAAAALYFVEKALDELRRGNTQVWTPFEVPKEAI
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCHHHH
SCGWTEAVRGVLSHHMVIRDGKIANYQPWPPTPWNANPRDIYGTPGPYEDAVQNTPIFEE
HCCHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
NGPDNFKGIDIMRTVRSFDPCLPCGVHMYLGNGKVIKQVHSPTMLPEI
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AEAGTTRELVEMSWDPITRIVGSLGIYTKIDFKQRRVVECYSTSSIFRGYSVFMRGKDP
CCCCCCHHHHHCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCEEEEECCCCC
RDAHFITSRICGICGDNHATCSVYNQNMAYGVAPPPLGEWIINLGEAAEYMFDHNIFQEN
CHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVGVDFCERMVRETNPSVWEKAQQTAAPHAHLHGYRTIADIMRALNPLEGEFYRQALQMS
CCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RMTREMFCLMEGRHVHPSTLYPGGVGTVPTVQLFTDYLTRLMRYIEFMKKAVPLHDDLFD
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FFYQALPGYEEVGRRRILLGCWGAFNDPEYCDFKYEHMTEWGRRMFVTPGVIVDGKLVTN
HHHHHCCCHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCEEEECCCEEECCEEEEC
DLVQINLGIRILLGSSYYEDWEDQEMFVTRDPLGNPVDRRHPWNVHTIPKPQKRDFSDKY
CEEEEECCEEEEECCCHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
SWVMSPRWFDGENYLALDTGGGPIARLWSTALSGLVDIGYIKATGSSVIINLPKTATMPE
HEEECCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCC
MTFEWKIPQWSNTIERDRARTYFQAYAAAAALYFVEKALDELRRGNTQVWTPFEVPKEAI
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCHHHH
SCGWTEAVRGVLSHHMVIRDGKIANYQPWPPTPWNANPRDIYGTPGPYEDAVQNTPIFEE
HCCHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
NGPDNFKGIDIMRTVRSFDPCLPCGVHMYLGNGKVIKQVHSPTMLPEI
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]