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The map label for this gene is rhdA [H]

Identifier: 221633722

GI number: 221633722

Start: 1756464

End: 1757378

Strand: Reverse

Name: rhdA [H]

Synonym: trd_1749

Alternate gene names: 221633722

Gene position: 1757378-1756464 (Counterclockwise)

Preceding gene: 221633723

Following gene: 221633721

Centisome position: 87.74

GC content: 65.79

Gene sequence:

>915_bases
GTGATCGCTGGTCTCGCGGTATTGGGGCTGTCTGCCTGCCGGCAGACGCGCGCGCAACGCTCCCTGGCTCCGGCCGAGCA
GTATCCTGGTGGGCGACTCCTGATCGAGGCACCGGAACTAGCTGACCGGATCGGGGAGCGCTCGCTCGTCATCCTCGATG
CCAGCCGGCTCCACGAGTACCATGCCGGGCATATTCCCGGGGCTCGTCACGTCTGGTGGCAAGATACGATGGAAATTCAC
AACGAGGTGTACGGGATGTTGGTCGGCGAGCCCCGACGGAGCCAACTCGTCCAGCGTATCGGGCTCGATCCTGACGACGA
AGTCGTCGTCTACGAGCGTGGCGACGGGCGGGCCGCCTGCCGCTGGTTGTGGTTTCTTCACGCGATCGGCTTTCGGCGAG
TACGCCTGCTGCACGGCGGCCTTCGGGCTTGGCTCGCGGCTGGTTACCCGACGACGCGTGCCCTTCCGCCCGCTGCGGAG
CACGGAACGTTTCAGCCCGTGCTCCACTACGATGTACTGGCCGAGCTACCGGACGTCCAGGCCGCACTCCAGGATCCGGA
ATCCTGCCTCCTCGATAACCGAACCGATCAGGAGCGCCGCCAAACCTGGGGTGGTCGTCTCCGGCTCGGCCAGATCCCCG
GCGCGGTTGCCGTGCCGTGGAGAGCGCTCCTGGCCGGGGACCCACCGCTCGCCTTCCGTCCACCGGACGAACTGGCCGCA
CTCTACCGGTCAGTCAGCGTCCAACCGGAGCAGCGGGTGATCGTCTACGGCTTGTCCAGTCCGCACGCGGCCATCAGCTA
TGTCTCACTGCGTCTCCTCGACTATCCGAACGTGCGCATCTACGATGGATCGTGGGCACAATGGGGGATGCTGAACGACC
TTCCGATCGAGCCAGCCGATGTCTCGAAGCCGTGA

Upstream 100 bases:

>100_bases
AAGGAGGAGGCATCTCCCCTTGCCGGTCTCGTGCATGGGCGCGAGACCGGCTCGCGGAGCGATGGCGGCGCGGAGCATGC
GGACGACCCGGCGAGGGCTC

Downstream 100 bases:

>100_bases
TCGAGGATCCAGCTGGCTCCACGGTTCGGCACGTCTACGATCCCGTGATCGGGCAGTGGCGGACGTACCGCCATCCGGCC
GCAGTCAGTCCGTGGCCAGC

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MIAGLAVLGLSACRQTRAQRSLAPAEQYPGGRLLIEAPELADRIGERSLVILDASRLHEYHAGHIPGARHVWWQDTMEIH
NEVYGMLVGEPRRSQLVQRIGLDPDDEVVVYERGDGRAACRWLWFLHAIGFRRVRLLHGGLRAWLAAGYPTTRALPPAAE
HGTFQPVLHYDVLAELPDVQAALQDPESCLLDNRTDQERRQTWGGRLRLGQIPGAVAVPWRALLAGDPPLAFRPPDELAA
LYRSVSVQPEQRVIVYGLSSPHAAISYVSLRLLDYPNVRIYDGSWAQWGMLNDLPIEPADVSKP

Sequences:

>Translated_304_residues
MIAGLAVLGLSACRQTRAQRSLAPAEQYPGGRLLIEAPELADRIGERSLVILDASRLHEYHAGHIPGARHVWWQDTMEIH
NEVYGMLVGEPRRSQLVQRIGLDPDDEVVVYERGDGRAACRWLWFLHAIGFRRVRLLHGGLRAWLAAGYPTTRALPPAAE
HGTFQPVLHYDVLAELPDVQAALQDPESCLLDNRTDQERRQTWGGRLRLGQIPGAVAVPWRALLAGDPPLAFRPPDELAA
LYRSVSVQPEQRVIVYGLSSPHAAISYVSLRLLDYPNVRIYDGSWAQWGMLNDLPIEPADVSKP
>Mature_304_residues
MIAGLAVLGLSACRQTRAQRSLAPAEQYPGGRLLIEAPELADRIGERSLVILDASRLHEYHAGHIPGARHVWWQDTMEIH
NEVYGMLVGEPRRSQLVQRIGLDPDDEVVVYERGDGRAACRWLWFLHAIGFRRVRLLHGGLRAWLAAGYPTTRALPPAAE
HGTFQPVLHYDVLAELPDVQAALQDPESCLLDNRTDQERRQTWGGRLRLGQIPGAVAVPWRALLAGDPPLAFRPPDELAA
LYRSVSVQPEQRVIVYGLSSPHAAISYVSLRLLDYPNVRIYDGSWAQWGMLNDLPIEPADVSKP

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=240, Percent_Identity=30.4166666666667, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI61835204, Length=245, Percent_Identity=27.7551020408163, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI194473681, Length=245, Percent_Identity=27.7551020408163, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI194473668, Length=245, Percent_Identity=27.7551020408163, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI87082121, Length=275, Percent_Identity=29.8181818181818, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081967, Length=246, Percent_Identity=27.2357723577236, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 33908; Mature: 33908

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGLAVLGLSACRQTRAQRSLAPAEQYPGGRLLIEAPELADRIGERSLVILDASRLHEY
CCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECHHHHHHCCCCEEEEEEHHHHHHH
HAGHIPGARHVWWQDTMEIHNEVYGMLVGEPRRSQLVQRIGLDPDDEVVVYERGDGRAAC
HCCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCCCCEEEEECCCCHHHH
RWLWFLHAIGFRRVRLLHGGLRAWLAAGYPTTRALPPAAEHGTFQPVLHYDVLAELPDVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHH
AALQDPESCLLDNRTDQERRQTWGGRLRLGQIPGAVAVPWRALLAGDPPLAFRPPDELAA
HHHCCCHHHHCCCCCCHHHHHHCCCEEEECCCCCCHHHCHHHHHCCCCCCCCCCHHHHHH
LYRSVSVQPEQRVIVYGLSSPHAAISYVSLRLLDYPNVRIYDGSWAQWGMLNDLPIEPAD
HHHHCCCCCCCEEEEEECCCCHHHHHHHEEEEECCCCEEEECCCCCCCCCCCCCCCCCCC
VSKP
CCCC
>Mature Secondary Structure
MIAGLAVLGLSACRQTRAQRSLAPAEQYPGGRLLIEAPELADRIGERSLVILDASRLHEY
CCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECHHHHHHCCCCEEEEEEHHHHHHH
HAGHIPGARHVWWQDTMEIHNEVYGMLVGEPRRSQLVQRIGLDPDDEVVVYERGDGRAAC
HCCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCCCCEEEEECCCCHHHH
RWLWFLHAIGFRRVRLLHGGLRAWLAAGYPTTRALPPAAEHGTFQPVLHYDVLAELPDVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHH
AALQDPESCLLDNRTDQERRQTWGGRLRLGQIPGAVAVPWRALLAGDPPLAFRPPDELAA
HHHCCCHHHHCCCCCCHHHHHHCCCEEEECCCCCCHHHCHHHHHCCCCCCCCCCHHHHHH
LYRSVSVQPEQRVIVYGLSSPHAAISYVSLRLLDYPNVRIYDGSWAQWGMLNDLPIEPAD
HHHHCCCCCCCEEEEEECCCCHHHHHHHEEEEECCCCEEEECCCCCCCCCCCCCCCCCCC
VSKP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]