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The map label for this gene is ispD [H]

Identifier: 221633697

GI number: 221633697

Start: 1728939

End: 1729634

Strand: Reverse

Name: ispD [H]

Synonym: trd_1724

Alternate gene names: 221633697

Gene position: 1729634-1728939 (Counterclockwise)

Preceding gene: 221633698

Following gene: 221633696

Centisome position: 86.35

GC content: 66.81

Gene sequence:

>696_bases
ATGACGTGCAGTGCGGTCGTTCCGGCGGCGGGGCGAGGTCAACGACTCGGCGGGCGCGAGAAACCGCTGCTCCCGCTGGC
TGGTCGTCCTGCCTTGGCCTGGGTCCTGGAGGCACTGAGTGCTTCCGGTGTCATCGCCGAGATCATCGTCGTGGCCAGCG
AGGCGAATCGGGAAGCGGTCGAGTCGCTGTGCACTGCGCTCGGGCTGTCGCTTCCTGTCCAGGTCGTGATCGGTGGAGCG
GAGCGTGCGCTCTCGGTCCGAGCGGGGGTGGAGCACGTACCGGACGACAGACGGTATGTGCTGATCCATGATGCCGCTCG
GCCGCTCGTGACGCCCGAGCTCGTCCGGCGAGCCATCGCAGCAGCGCTGCGACACGGCGCAGCGGTCGCAGCGATTCCGG
TGACCGACACGATCAAGCAGGTGGCCAGCGATGGGCGAGTCGTGACGACCCCGGAACGCTCGACCCTGGTAGCGGCGCAA
ACGCCACAAGTCTTTCGGTTGGACTGGCTCCGCGAAGCGTACCGCCGGGCCGGTGCGCAGTGGGTGACCGCCACGGACGA
AGCGATGCTGCTCGAACGTGCTGGCTTTCCAGTCTTTGTCTTTCCGGGCGATCCCGAGAATCTCAAGCTGACCACGCCGA
TCGACGTGACGATTGCCGAGCTGATCCTCGAGCGCAGGATGCGGGGGAACGCATGA

Upstream 100 bases:

>100_bases
CGCGCATCGGCCAGGACGTGCCAGTCGAGGTAACGCGGATCCTGCGGACACCAGCTGGACGCTTGGTCTTCGCGCAGTTG
GTCGCGCAGAGCGAGCCCTG

Downstream 100 bases:

>100_bases
TACGAGTTGGTTTGGGTTACGACGTCCATCCACTCGTGCCGGGGCGGCGGCTAGTGCTGGGTGGTGTCGAGATCCCGGGA
ACGGTCGGCTTAGCTGGACA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTCSAVVPAAGRGQRLGGREKPLLPLAGRPALAWVLEALSASGVIAEIIVVASEANREAVESLCTALGLSLPVQVVIGGA
ERALSVRAGVEHVPDDRRYVLIHDAARPLVTPELVRRAIAAALRHGAAVAAIPVTDTIKQVASDGRVVTTPERSTLVAAQ
TPQVFRLDWLREAYRRAGAQWVTATDEAMLLERAGFPVFVFPGDPENLKLTTPIDVTIAELILERRMRGNA

Sequences:

>Translated_231_residues
MTCSAVVPAAGRGQRLGGREKPLLPLAGRPALAWVLEALSASGVIAEIIVVASEANREAVESLCTALGLSLPVQVVIGGA
ERALSVRAGVEHVPDDRRYVLIHDAARPLVTPELVRRAIAAALRHGAAVAAIPVTDTIKQVASDGRVVTTPERSTLVAAQ
TPQVFRLDWLREAYRRAGAQWVTATDEAMLLERAGFPVFVFPGDPENLKLTTPIDVTIAELILERRMRGNA
>Mature_230_residues
TCSAVVPAAGRGQRLGGREKPLLPLAGRPALAWVLEALSASGVIAEIIVVASEANREAVESLCTALGLSLPVQVVIGGAE
RALSVRAGVEHVPDDRRYVLIHDAARPLVTPELVRRAIAAALRHGAAVAAIPVTDTIKQVASDGRVVTTPERSTLVAAQT
PQVFRLDWLREAYRRAGAQWVTATDEAMLLERAGFPVFVFPGDPENLKLTTPIDVTIAELILERRMRGNA

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=235, Percent_Identity=28.5106382978723, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=34.9344978165939, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 24642; Mature: 24511

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCSAVVPAAGRGQRLGGREKPLLPLAGRPALAWVLEALSASGVIAEIIVVASEANREAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
ESLCTALGLSLPVQVVIGGAERALSVRAGVEHVPDDRRYVLIHDAARPLVTPELVRRAIA
HHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHH
AALRHGAAVAAIPVTDTIKQVASDGRVVTTPERSTLVAAQTPQVFRLDWLREAYRRAGAQ
HHHHCCCEEEEECCHHHHHHHHCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCE
WVTATDEAMLLERAGFPVFVFPGDPENLKLTTPIDVTIAELILERRMRGNA
EEEECHHHHHHHHCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TCSAVVPAAGRGQRLGGREKPLLPLAGRPALAWVLEALSASGVIAEIIVVASEANREAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
ESLCTALGLSLPVQVVIGGAERALSVRAGVEHVPDDRRYVLIHDAARPLVTPELVRRAIA
HHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHH
AALRHGAAVAAIPVTDTIKQVASDGRVVTTPERSTLVAAQTPQVFRLDWLREAYRRAGAQ
HHHHCCCEEEEECCHHHHHHHHCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCE
WVTATDEAMLLERAGFPVFVFPGDPENLKLTTPIDVTIAELILERRMRGNA
EEEECHHHHHHHHCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA