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The map label for this gene is ispF [H]

Identifier: 221633696

GI number: 221633696

Start: 1728433

End: 1728942

Strand: Reverse

Name: ispF [H]

Synonym: trd_1723

Alternate gene names: 221633696

Gene position: 1728942-1728433 (Counterclockwise)

Preceding gene: 221633697

Following gene: 221633695

Centisome position: 86.32

GC content: 65.88

Gene sequence:

>510_bases
ATGATACGAGTTGGTTTGGGTTACGACGTCCATCCACTCGTGCCGGGGCGGCGGCTAGTGCTGGGTGGTGTCGAGATCCC
GGGAACGGTCGGCTTAGCTGGACACTCGGATGCCGATGTCTTGGCTCATGCGATCGGCGATGCGCTGCTGGGGGCGGCTG
GACTCGGTGACCTGGGGACGCATTTCCCACCGGACGACGAGCGGTGGCGGGATGCCTCGAGTCTCGATCTCCTCCGCCGC
ATCCGGGAGGAGCTCGGCCGAGTTGGCTGGCGCGTGATCTTCGTCGATGCGACGGTCATCGCCGAGTTTCCCCGCATCGC
ACCCTTTCGAGAACGGATGCGCGAGCGGTTGGCTGCGGCGTTGTCCCTCGCCGTCGAGCAGATCAGCGTGAAGGCGACGA
CGAACGAGCGACTCGGGTTCATCGGGCGTGGTGAGGGGATCGCTGCGCTGGCCGTGGCGATGCTCCAGCGCAGCGACGAG
GAACACGACTCCGATCCGGACGGGAGGTAA

Upstream 100 bases:

>100_bases
TCTTTGTCTTTCCGGGCGATCCCGAGAATCTCAAGCTGACCACGCCGATCGACGTGACGATTGCCGAGCTGATCCTCGAG
CGCAGGATGCGGGGGAACGC

Downstream 100 bases:

>100_bases
GGACAGTGCGGGTTCTCCTCCAGCGGGTGAGCGAAGCCAGTGTCACGGTTGATGGAACGTTGGTGAGTTCGATCGGCCAG
GGAGTCCTGCTCTTGGTCGG

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MIRVGLGYDVHPLVPGRRLVLGGVEIPGTVGLAGHSDADVLAHAIGDALLGAAGLGDLGTHFPPDDERWRDASSLDLLRR
IREELGRVGWRVIFVDATVIAEFPRIAPFRERMRERLAAALSLAVEQISVKATTNERLGFIGRGEGIAALAVAMLQRSDE
EHDSDPDGR

Sequences:

>Translated_169_residues
MIRVGLGYDVHPLVPGRRLVLGGVEIPGTVGLAGHSDADVLAHAIGDALLGAAGLGDLGTHFPPDDERWRDASSLDLLRR
IREELGRVGWRVIFVDATVIAEFPRIAPFRERMRERLAAALSLAVEQISVKATTNERLGFIGRGEGIAALAVAMLQRSDE
EHDSDPDGR
>Mature_169_residues
MIRVGLGYDVHPLVPGRRLVLGGVEIPGTVGLAGHSDADVLAHAIGDALLGAAGLGDLGTHFPPDDERWRDASSLDLLRR
IREELGRVGWRVIFVDATVIAEFPRIAPFRERMRERLAAALSLAVEQISVKATTNERLGFIGRGEGIAALAVAMLQRSDE
EHDSDPDGR

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=159, Percent_Identity=44.6540880503145, Blast_Score=124, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 18233; Mature: 18233

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVGLGYDVHPLVPGRRLVLGGVEIPGTVGLAGHSDADVLAHAIGDALLGAAGLGDLGT
CEEECCCCCCCCCCCCCEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
HFPPDDERWRDASSLDLLRRIREELGRVGWRVIFVDATVIAEFPRIAPFRERMRERLAAA
CCCCCCHHHCCHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHCCCCCHHHHHHHHHHHHH
LSLAVEQISVKATTNERLGFIGRGEGIAALAVAMLQRSDEEHDSDPDGR
HHHHHHHHHEEECCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIRVGLGYDVHPLVPGRRLVLGGVEIPGTVGLAGHSDADVLAHAIGDALLGAAGLGDLGT
CEEECCCCCCCCCCCCCEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
HFPPDDERWRDASSLDLLRRIREELGRVGWRVIFVDATVIAEFPRIAPFRERMRERLAAA
CCCCCCHHHCCHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHCCCCCHHHHHHHHHHHHH
LSLAVEQISVKATTNERLGFIGRGEGIAALAVAMLQRSDEEHDSDPDGR
HHHHHHHHHEEECCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA