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The map label for this gene is nrdF [H]

Identifier: 221633388

GI number: 221633388

Start: 1400086

End: 1401072

Strand: Reverse

Name: nrdF [H]

Synonym: trd_1408

Alternate gene names: 221633388

Gene position: 1401072-1400086 (Counterclockwise)

Preceding gene: 221633389

Following gene: 221633374

Centisome position: 69.95

GC content: 60.49

Gene sequence:

>987_bases
ATGGCCCGCCGATTGCTCGGAGGCGATCCCCTAGCTGCTGTGGAGCTCTTCCCGCTGAGTCACCCGTGGGCCTATGCGCA
TGTCCAACAAGCCAAGCACAACACCTGGTTCCCGGAAGAGGTTCCGCTGCACGATGACGTACGCGACTGGCATGAACGCT
TGAGCGATGAAGAGCGGCGAGCGGTGGAAATGTTCCTGGGTTTCTTCAATCCGATGGAGTCGCTGGTCACGACCAACCTG
CTGCTCAGTCTCTATCGGGCGGTGACGGCCCCGGAAGCTCGGCTGTATTTGGCTCGACAGGCGTGGGAAGAAGCCAACCA
CGTCATGGCGTTCGAGTACGTCATCAAGACGCTGCCGTTGGACCGGGAGCGGGTGTTCGGCTTGCACGTGGAGCATCCAG
CTGTCGCGGCGAAAGAGCTATTCCAGCGTCAGCTGGCCGAGCAGTGGCTCGTTACCCCTGACGTCGAGTCTGTCGAGGGA
CTGCAGCGACTCGTCCGCAACCTCGTCGGCTACTTCGTGATCTTGGAGGGAATCTTCTTCTACTCCGGCTTCGCGCTGGC
GCTCGCGTTTCGGCAGCGAAACCTGTTGCGTGGTTTGTGCACCATCGTCGATTGGGTCCTGAAGGACGAGTCGCTCCACC
TCTCGTTCGGTATCCACTTGATCACGACGATCCTGGAGGAGCATCCGGAAATCATGAGCGCGGCGTTCGGTCGCCAGCTG
CGGGAGCTCATCCTACGTGCGGTCGAGCTCGAAGAAGCGTACAACCGGGCAGTACTCCCGGAGCCGTTGATCGGTCTAGA
CGCTGATGTGCTCAACCGGTACGTGCGCTACATCGCTGATCGCCGGTTGGAAGAACTGGGTTTGCCGATCGCTTTCGGTG
AACCGAATCCGTTGCGTTGGATGGTCGCGCAGGTCGATATGCCGGAGATCGTCAACTTCTTCGAGGCCCGCAATATCCAT
TACGAGGTCGGCCGCGCGCGCTGGTAG

Upstream 100 bases:

>100_bases
GTGCAGCTCGCCGAGCGCGAGGTGCTGGCGAGCGATGCGGCGAGCTGCCGTCTCGATGGGTCGTGCGAAAGTTGCCAGTG
AAGGACGAGGAGGGACCAGA

Downstream 100 bases:

>100_bases
AGTGGATGTGCAGTGCCCGACGAAGGCGAGCGGGGAGCAGTCGTGCCGCTCCCCGCTCGTGTGTGTGGTGCATCTCAGCT
CTTCCACGTCGGCTTGGGAA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MARRLLGGDPLAAVELFPLSHPWAYAHVQQAKHNTWFPEEVPLHDDVRDWHERLSDEERRAVEMFLGFFNPMESLVTTNL
LLSLYRAVTAPEARLYLARQAWEEANHVMAFEYVIKTLPLDRERVFGLHVEHPAVAAKELFQRQLAEQWLVTPDVESVEG
LQRLVRNLVGYFVILEGIFFYSGFALALAFRQRNLLRGLCTIVDWVLKDESLHLSFGIHLITTILEEHPEIMSAAFGRQL
RELILRAVELEEAYNRAVLPEPLIGLDADVLNRYVRYIADRRLEELGLPIAFGEPNPLRWMVAQVDMPEIVNFFEARNIH
YEVGRARW

Sequences:

>Translated_328_residues
MARRLLGGDPLAAVELFPLSHPWAYAHVQQAKHNTWFPEEVPLHDDVRDWHERLSDEERRAVEMFLGFFNPMESLVTTNL
LLSLYRAVTAPEARLYLARQAWEEANHVMAFEYVIKTLPLDRERVFGLHVEHPAVAAKELFQRQLAEQWLVTPDVESVEG
LQRLVRNLVGYFVILEGIFFYSGFALALAFRQRNLLRGLCTIVDWVLKDESLHLSFGIHLITTILEEHPEIMSAAFGRQL
RELILRAVELEEAYNRAVLPEPLIGLDADVLNRYVRYIADRRLEELGLPIAFGEPNPLRWMVAQVDMPEIVNFFEARNIH
YEVGRARW
>Mature_327_residues
ARRLLGGDPLAAVELFPLSHPWAYAHVQQAKHNTWFPEEVPLHDDVRDWHERLSDEERRAVEMFLGFFNPMESLVTTNLL
LSLYRAVTAPEARLYLARQAWEEANHVMAFEYVIKTLPLDRERVFGLHVEHPAVAAKELFQRQLAEQWLVTPDVESVEGL
QRLVRNLVGYFVILEGIFFYSGFALALAFRQRNLLRGLCTIVDWVLKDESLHLSFGIHLITTILEEHPEIMSAAFGRQLR
ELILRAVELEEAYNRAVLPEPLIGLDADVLNRYVRYIADRRLEELGLPIAFGEPNPLRWMVAQVDMPEIVNFFEARNIHY
EVGRARW

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=310, Percent_Identity=29.0322580645161, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI289177074, Length=310, Percent_Identity=29.0322580645161, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI260064013, Length=310, Percent_Identity=29.0322580645161, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4557845, Length=310, Percent_Identity=29.0322580645161, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI289177076, Length=287, Percent_Identity=28.9198606271777, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1788567, Length=293, Percent_Identity=24.9146757679181, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17551968, Length=310, Percent_Identity=26.4516129032258, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193206379, Length=300, Percent_Identity=25.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17567153, Length=160, Percent_Identity=31.25, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322434, Length=322, Percent_Identity=28.5714285714286, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321619, Length=315, Percent_Identity=21.9047619047619, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI18079279, Length=310, Percent_Identity=29.3548387096774, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38074; Mature: 37943

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRLLGGDPLAAVELFPLSHPWAYAHVQQAKHNTWFPEEVPLHDDVRDWHERLSDEERR
CCCCCCCCCCCHHEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCHHHHH
AVEMFLGFFNPMESLVTTNLLLSLYRAVTAPEARLYLARQAWEEANHVMAFEYVIKTLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
DRERVFGLHVEHPAVAAKELFQRQLAEQWLVTPDVESVEGLQRLVRNLVGYFVILEGIFF
CHHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YSGFALALAFRQRNLLRGLCTIVDWVLKDESLHLSFGIHLITTILEEHPEIMSAAFGRQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHHHH
RELILRAVELEEAYNRAVLPEPLIGLDADVLNRYVRYIADRRLEELGLPIAFGEPNPLRW
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEE
MVAQVDMPEIVNFFEARNIHYEVGRARW
EEECCCHHHHHHHHHHCCCHHHCCCCCC
>Mature Secondary Structure 
ARRLLGGDPLAAVELFPLSHPWAYAHVQQAKHNTWFPEEVPLHDDVRDWHERLSDEERR
CCCCCCCCCCHHEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCHHHHH
AVEMFLGFFNPMESLVTTNLLLSLYRAVTAPEARLYLARQAWEEANHVMAFEYVIKTLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
DRERVFGLHVEHPAVAAKELFQRQLAEQWLVTPDVESVEGLQRLVRNLVGYFVILEGIFF
CHHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YSGFALALAFRQRNLLRGLCTIVDWVLKDESLHLSFGIHLITTILEEHPEIMSAAFGRQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHHHH
RELILRAVELEEAYNRAVLPEPLIGLDADVLNRYVRYIADRRLEELGLPIAFGEPNPLRW
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEE
MVAQVDMPEIVNFFEARNIHYEVGRARW
EEECCCHHHHHHHHHHCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]