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The map label for this gene is yagI [H]

Identifier: 221633374

GI number: 221633374

Start: 1383323

End: 1384144

Strand: Reverse

Name: yagI [H]

Synonym: trd_1394

Alternate gene names: 221633374

Gene position: 1384144-1383323 (Counterclockwise)

Preceding gene: 221633388

Following gene: 221633373

Centisome position: 69.1

GC content: 63.5

Gene sequence:

>822_bases
ATGCTCCAGACCGTGCAAAAGGCAGCACAAGTGCTTCGCCTGTTTACGCCGCACCAGCCTGAATGGGGGGTCACCGAGGT
CGCCGCAGCGCTGGAGATCCCCAAGTCGGGTGCTCATGCGCTTCTGCGTACCTTGGCAGCGGAAGGTCTCTTGCAGCGAA
CGGCCGCTGGCCGTTACCGTCTCGGCTGGGCGCTGTTCGAGCTGAGTCAGACGCTGCTCGACTCGAGTGCGCTCCTCCGC
GCGGCCCGTCCGGTCATGGAGCGTCTGGTCGCTGGGTGGGGCGAGACGACGCACCTTGCCGTGTTGGTCGATGGACAAGT
GTTGTACGTCGAAAAGCTTCAAGGCGATCGTGCCCTCGAGATCGTTCTGTCCGGAGTCGGCAAGCGTCTCCCAGCGCACT
GCTCTGGCGTCGGAAAGGTGCTGCTTGCCCATCAGCCATGGGAGGTCGTGCTCGGGATCGTCGAGCGGACCGGTCTGGTC
AGTTTCACGCCGAACACGATCAAGTCGGTCGAGCAGCTGCGCGAAGAACTGGAACGGGTCCGCCAGCAGGGGTTCGCCTA
TGACCAGGAAGAGGTGATGGTTGGGCTCTGTTGTGCGGCCGCGCCGATCCGCGACGAGAGCGGTCACGTCATCGCGGCGA
TGAGTCTCTCGGTGCCGGCCTACCGCTTCTATCCGAACCGACAGCGGCTCACCACCGCGATCGTCGATGCTGCGCGACGG
GTTTCGGAAGAACTCGGGTACTATCAGGAGGATTGGGCATGGGAGAACGGCAAGCGGTCAAGGTCGCGATCCTCGGTACC
GGGAATATCGGTACCGACCTGA

Upstream 100 bases:

>100_bases
CCCGTGAGCGAGCCAGCGGACGGCGTGTGGTTCAGCCATCGTGAACGAGCTGCTTGCTGGTTTGCCAACGTGCTGGTATC
GTGATGGTGTGGAGGGTGCG

Downstream 100 bases:

>100_bases
TGTACAAGCTCTTGCGGAATCCGGGCCACATGGAATTGGCCATGTTCGCCGGGATCGATCCGCAGTCGGAGGGGATCGCC
CGCGCCAAGAAACTAGGTAT

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MLQTVQKAAQVLRLFTPHQPEWGVTEVAAALEIPKSGAHALLRTLAAEGLLQRTAAGRYRLGWALFELSQTLLDSSALLR
AARPVMERLVAGWGETTHLAVLVDGQVLYVEKLQGDRALEIVLSGVGKRLPAHCSGVGKVLLAHQPWEVVLGIVERTGLV
SFTPNTIKSVEQLREELERVRQQGFAYDQEEVMVGLCCAAAPIRDESGHVIAAMSLSVPAYRFYPNRQRLTTAIVDAARR
VSEELGYYQEDWAWENGKRSRSRSSVPGISVPT

Sequences:

>Translated_273_residues
MLQTVQKAAQVLRLFTPHQPEWGVTEVAAALEIPKSGAHALLRTLAAEGLLQRTAAGRYRLGWALFELSQTLLDSSALLR
AARPVMERLVAGWGETTHLAVLVDGQVLYVEKLQGDRALEIVLSGVGKRLPAHCSGVGKVLLAHQPWEVVLGIVERTGLV
SFTPNTIKSVEQLREELERVRQQGFAYDQEEVMVGLCCAAAPIRDESGHVIAAMSLSVPAYRFYPNRQRLTTAIVDAARR
VSEELGYYQEDWAWENGKRSRSRSSVPGISVPT
>Mature_273_residues
MLQTVQKAAQVLRLFTPHQPEWGVTEVAAALEIPKSGAHALLRTLAAEGLLQRTAAGRYRLGWALFELSQTLLDSSALLR
AARPVMERLVAGWGETTHLAVLVDGQVLYVEKLQGDRALEIVLSGVGKRLPAHCSGVGKVLLAHQPWEVVLGIVERTGLV
SFTPNTIKSVEQLREELERVRQQGFAYDQEEVMVGLCCAAAPIRDESGHVIAAMSLSVPAYRFYPNRQRLTTAIVDAARR
VSEELGYYQEDWAWENGKRSRSRSSVPGISVPT

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=223, Percent_Identity=35.4260089686099, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786715, Length=246, Percent_Identity=31.3008130081301, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788131, Length=248, Percent_Identity=33.8709677419355, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI87082359, Length=249, Percent_Identity=29.718875502008, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788581, Length=248, Percent_Identity=34.2741935483871, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789999, Length=221, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1790752, Length=227, Percent_Identity=27.3127753303965, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30062; Mature: 30062

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTVQKAAQVLRLFTPHQPEWGVTEVAAALEIPKSGAHALLRTLAAEGLLQRTAAGRYR
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHH
LGWALFELSQTLLDSSALLRAARPVMERLVAGWGETTHLAVLVDGQVLYVEKLQGDRALE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEECCCCHHHH
IVLSGVGKRLPAHCSGVGKVLLAHQPWEVVLGIVERTGLVSFTPNTIKSVEQLREELERV
HHHHHHCCCCCHHHHHHHHEEECCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHH
RQQGFAYDQEEVMVGLCCAAAPIRDESGHVIAAMSLSVPAYRFYPNRQRLTTAIVDAARR
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHEECCCHHHHHHHHHHHHHH
VSEELGYYQEDWAWENGKRSRSRSSVPGISVPT
HHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLQTVQKAAQVLRLFTPHQPEWGVTEVAAALEIPKSGAHALLRTLAAEGLLQRTAAGRYR
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHH
LGWALFELSQTLLDSSALLRAARPVMERLVAGWGETTHLAVLVDGQVLYVEKLQGDRALE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEECCCCHHHH
IVLSGVGKRLPAHCSGVGKVLLAHQPWEVVLGIVERTGLVSFTPNTIKSVEQLREELERV
HHHHHHCCCCCHHHHHHHHEEECCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHH
RQQGFAYDQEEVMVGLCCAAAPIRDESGHVIAAMSLSVPAYRFYPNRQRLTTAIVDAARR
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHEECCCHHHHHHHHHHHHHH
VSEELGYYQEDWAWENGKRSRSRSSVPGISVPT
HHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]