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The map label for this gene is prs [H]

Identifier: 221633355

GI number: 221633355

Start: 1367256

End: 1368230

Strand: Reverse

Name: prs [H]

Synonym: trd_1375

Alternate gene names: 221633355

Gene position: 1368230-1367256 (Counterclockwise)

Preceding gene: 221633356

Following gene: 221633354

Centisome position: 68.31

GC content: 59.28

Gene sequence:

>975_bases
ATGGGCGGGCTCTACGACCAACTGACGATTTTCGGTGGTAATTCCAATCCGGAACTCACCAAGGCGATCTGCGCCTATAT
CGACATCCCACCCGGACGGATCGAAGTCTTCAAGTTCAGCAACGAAAATATCTTCGTGCGCATCGGCGAGAGCGTGCGCG
AGAACGATGTCTTCGTCATCCAGTCGTTCTCGAGCCCCGTGAACGAGAGCATCATGGAGCTACTGATCATCATCGATGCC
CTCAAGCGCGCATCAGCCGGTCGGATCACCGCGGTGATCCCCTACTACGCCTACGGGAGAACAGACAAGAAGGACCAACC
GCGTGTGCCCATCACCGCTCGTCTGATCGCCGACATGATCACCGTCGCCGGAGCGGATCGGATTCTGACCGTCGATCTGC
ATGCAGGGCAGATCCAAGGTTTCTTCAACATCCCCGTGGACGAGATGACCGCGCTGCCGATTCTCGCGCGGTACTTCCAG
GAAAAACGACTGGAGAATCCGGTAGTCGTTTCCCCTGACCTCGGGAGCACCAAGCGGGCACGCAACTTCGCCGAGGAACT
GGACGCTCCCCTCGCCATCATCGAAAAGCGCCGGCTGGGGAACGACGATCGAACCGAAGTGCTGAATCTCATCGGTTCGG
TCGATGACGCATCGGTGATCCTGATCGACGACGAGATCGATACAGCGGGATCGATCACCCAAGCGGCCGAGCTCTGTATC
GCTCGCGGTGCGCGTGAAGTGTATGCGGCCTGTACCCATCCAGTCCTGAGCGGACCGGCGATCGAACGGCTCGCCCTGAG
CCCGATCAAAGAAGTTGTCGTGACCGATACGATCCCGCTTCCTCCACAGAAGCGGCTGGACAAGATCACGGTTCTTTCGG
TGGCACCCCTGCTCGGTGAATCGATCCAGCGGATCCACACCGGAGCCAGCGTCAGTTTGGCCTACCGCAGTGCTGGGCGT
TTGCCCTTGCGCTGA

Upstream 100 bases:

>100_bases
CCATACGGAGTGGGCCACGGCCCGAGCAAGCGGGCAGCGGAGCAAGAGGCAGCACGGCGCGCACTCGAGCGGCTGCGGGC
GGAGGGGATCGGCTGAGGCG

Downstream 100 bases:

>100_bases
GATCCACTGGTCGGGCGAACGGTGGCAGCGCTTGACGTCGCCCGGGTGAGCATGCAACGCTTCTCGGGCACGAAGCCGGA
CGGCAGTTGCGTGAGGTGTG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MGGLYDQLTIFGGNSNPELTKAICAYIDIPPGRIEVFKFSNENIFVRIGESVRENDVFVIQSFSSPVNESIMELLIIIDA
LKRASAGRITAVIPYYAYGRTDKKDQPRVPITARLIADMITVAGADRILTVDLHAGQIQGFFNIPVDEMTALPILARYFQ
EKRLENPVVVSPDLGSTKRARNFAEELDAPLAIIEKRRLGNDDRTEVLNLIGSVDDASVILIDDEIDTAGSITQAAELCI
ARGAREVYAACTHPVLSGPAIERLALSPIKEVVVTDTIPLPPQKRLDKITVLSVAPLLGESIQRIHTGASVSLAYRSAGR
LPLR

Sequences:

>Translated_324_residues
MGGLYDQLTIFGGNSNPELTKAICAYIDIPPGRIEVFKFSNENIFVRIGESVRENDVFVIQSFSSPVNESIMELLIIIDA
LKRASAGRITAVIPYYAYGRTDKKDQPRVPITARLIADMITVAGADRILTVDLHAGQIQGFFNIPVDEMTALPILARYFQ
EKRLENPVVVSPDLGSTKRARNFAEELDAPLAIIEKRRLGNDDRTEVLNLIGSVDDASVILIDDEIDTAGSITQAAELCI
ARGAREVYAACTHPVLSGPAIERLALSPIKEVVVTDTIPLPPQKRLDKITVLSVAPLLGESIQRIHTGASVSLAYRSAGR
LPLR
>Mature_323_residues
GGLYDQLTIFGGNSNPELTKAICAYIDIPPGRIEVFKFSNENIFVRIGESVRENDVFVIQSFSSPVNESIMELLIIIDAL
KRASAGRITAVIPYYAYGRTDKKDQPRVPITARLIADMITVAGADRILTVDLHAGQIQGFFNIPVDEMTALPILARYFQE
KRLENPVVVSPDLGSTKRARNFAEELDAPLAIIEKRRLGNDDRTEVLNLIGSVDDASVILIDDEIDTAGSITQAAELCIA
RGAREVYAACTHPVLSGPAIERLALSPIKEVVVTDTIPLPPQKRLDKITVLSVAPLLGESIQRIHTGASVSLAYRSAGRL
PLR

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=312, Percent_Identity=44.2307692307692, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI4506129, Length=312, Percent_Identity=43.5897435897436, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI28557709, Length=312, Percent_Identity=43.5897435897436, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI84875539, Length=315, Percent_Identity=43.1746031746032, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI4506133, Length=350, Percent_Identity=36.5714285714286, Blast_Score=187, Evalue=9e-48,
Organism=Homo sapiens, GI194018537, Length=343, Percent_Identity=35.2769679300292, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI310128524, Length=141, Percent_Identity=28.3687943262411, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI310115209, Length=141, Percent_Identity=28.3687943262411, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI310118259, Length=141, Percent_Identity=28.3687943262411, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI310119946, Length=141, Percent_Identity=28.3687943262411, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1787458, Length=311, Percent_Identity=45.9807073954984, Blast_Score=269, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=44.8387096774194, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI25149168, Length=312, Percent_Identity=44.2307692307692, Blast_Score=273, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI17554702, Length=312, Percent_Identity=44.2307692307692, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI71989924, Length=312, Percent_Identity=44.2307692307692, Blast_Score=270, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=33.4319526627219, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6320946, Length=316, Percent_Identity=41.1392405063291, Blast_Score=250, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319403, Length=321, Percent_Identity=39.8753894080997, Blast_Score=249, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6321776, Length=273, Percent_Identity=45.0549450549451, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6322667, Length=199, Percent_Identity=43.21608040201, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324511, Length=108, Percent_Identity=48.1481481481481, Blast_Score=109, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21355239, Length=312, Percent_Identity=44.2307692307692, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI45551540, Length=335, Percent_Identity=41.1940298507463, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=34.3661971830986, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=34.3661971830986, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=34.3661971830986, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=34.3661971830986, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45552010, Length=197, Percent_Identity=39.0862944162437, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24651462, Length=197, Percent_Identity=39.0862944162437, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24651464, Length=197, Percent_Identity=39.0862944162437, Blast_Score=138, Evalue=6e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35372; Mature: 35241

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGLYDQLTIFGGNSNPELTKAICAYIDIPPGRIEVFKFSNENIFVRIGESVRENDVFVI
CCCCCCEEEEEECCCCCHHHHHHHHHEECCCCEEEEEEECCCEEEEEECCCCCCCCEEEE
QSFSSPVNESIMELLIIIDALKRASAGRITAVIPYYAYGRTDKKDQPRVPITARLIADMI
ECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
TVAGADRILTVDLHAGQIQGFFNIPVDEMTALPILARYFQEKRLENPVVVSPDLGSTKRA
HHCCCCEEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHH
RNFAEELDAPLAIIEKRRLGNDDRTEVLNLIGSVDDASVILIDDEIDTAGSITQAAELCI
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHH
ARGAREVYAACTHPVLSGPAIERLALSPIKEVVVTDTIPLPPQKRLDKITVLSVAPLLGE
HHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCHHHHCHHHHHHHHHHHHH
SIQRIHTGASVSLAYRSAGRLPLR
HHHHHHCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
GGLYDQLTIFGGNSNPELTKAICAYIDIPPGRIEVFKFSNENIFVRIGESVRENDVFVI
CCCCCEEEEEECCCCCHHHHHHHHHEECCCCEEEEEEECCCEEEEEECCCCCCCCEEEE
QSFSSPVNESIMELLIIIDALKRASAGRITAVIPYYAYGRTDKKDQPRVPITARLIADMI
ECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
TVAGADRILTVDLHAGQIQGFFNIPVDEMTALPILARYFQEKRLENPVVVSPDLGSTKRA
HHCCCCEEEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHH
RNFAEELDAPLAIIEKRRLGNDDRTEVLNLIGSVDDASVILIDDEIDTAGSITQAAELCI
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHH
ARGAREVYAACTHPVLSGPAIERLALSPIKEVVVTDTIPLPPQKRLDKITVLSVAPLLGE
HHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCHHHHCHHHHHHHHHHHHH
SIQRIHTGASVSLAYRSAGRLPLR
HHHHHHCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]