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The map label for this gene is mrdA [H]

Identifier: 221633354

GI number: 221633354

Start: 1364975

End: 1367152

Strand: Reverse

Name: mrdA [H]

Synonym: trd_1373

Alternate gene names: 221633354

Gene position: 1367152-1364975 (Counterclockwise)

Preceding gene: 221633355

Following gene: 221633348

Centisome position: 68.26

GC content: 65.29

Gene sequence:

>2178_bases
ATGCCACGCGCTACGGTCCGTTCGTCGCGCCGGAGAAGCGCGTCGGGATCTCTGGTCGCTGGCCTGCTGATCTTCGCGAT
CGTGACCGGCGGTATCGTCGCAGTCGCGCTCCAGTTCGGTGACCGGCAGGACACGACGACGATCGGGGTTGCCGACCAGA
GCAGTGCCTCAGCCACACTGACGCGCGAGTTGGCTGCCCCGCGGACAGCCCGCGAAGCTGCTGAGCGTTTCGTGCGCGCC
TGGGAGGAGCGGGACTACCGCGCGATGTACGCGCTCCTCTCGTCGGCTGCCCGTCAGCAAATCGACGAAGCTCAGTTCAT
TCGTCGCTACGAAGCGATCTCGGCCGAAATGGGCGAACGCTCGCTCCAGGTCACGCTGGGAGAGGCGTTGCCTGGGACCA
TGCGCTTTCCGATCCACGTGGTACGCGAGACGACGCGCGTCGGCCGACTCGAGGAGGATAACGCGATCCCGATCGTTCGC
GAAGGGGACGCGTTCCGGATCGATTGGACACCTTCGCTCATCGTCGCCGACTTGGGTGACGGCCGTGTCGAGTGGATCCC
GACGGTTCCCCAGCGTGGCCGTCTCCTCGACGTGAAGGGAAGGCCGCTTGCCCAACTGGGTTTGGTGAACAAGGTGGGTG
TCGTTCCGGGACAGATTCGCGACGAGCACCTTTTGCTCGAGCGCTTGAGTACTCTCCTCAATGTGCCCTCCGAGACGATA
CGGCAGCGGTATGCTGGTGGACAACCTGATTGGTTCATGCCGGTCGCAACGCTCCCCGATCCGATCGATCCCGGTTTGCT
CGAACAGCTCGCCGGAATCCCCGGGGTCGTGGTCCGTCAGTGGCCCGAGCGCGTCTATCCCCTCGGCCCGGCCGCCGCGC
ACGTGACCGGCTATCTCTCGGAGGTTTCAGCCGAGGAACTCGAGCGGCGTGCGGCTGACGGCTATGAGCCGGGCGATCGG
ATCGGACGAGCGGGGATCGAGGCGTGGGCGGAAGACTTCCTGCGCGGCCGCCGCGGAGGGCGGTTAGTCATCGTCGGGCC
GGACGGGAGAGAGCGACGATTCCTCGCCGAGATCCCGGCACAGCCGGCGGCTGACGTGCTGACGACGATCGATACCGACC
TGCAAGAGGCAGCGTACCGAGCGCTCGGTGATCGGGTCGGGAGCGTGGTGGCGATCGATCCGCGGAGCGGTGCCATCCGG
GCTTTGGTGAGTAACCCGAGCTTCGATCCCAACCGCTTCATCCTCGGGTTGCGAACCGACGAATGGCAGGCGCTCAATGA
CGAGCAACGTCGCCCACTGATCGACCGGGCGACGCAGGTGGGATATCCCACCGGATCGACCTTCAAGGTGGTGACCATGG
CTGCAGGACTGGCGTATCTGGGCTTGCGCGCCGAGAGCAGGTTCGATTGCCCGCCCACCTTCAGTCTGCCGGGAAGTTCT
GTTGTTTGGCGCGATTGGAACCCGCGCGGGCAGGGACAGTTGACGCTGCACGATGCCCTGGTCCAGTCCTGTAACACCGT
CTTCTTTCAGATCGGTGCGCAGCTCGACGAGCGCGATCCCAACCTGCTTCCCCAGATGGCGCGTGCGTTCGGTTTCGGGT
CGGAAACTGGACTCACCGAGTTGCCCGAGACGGCCGGCCTGGTCCCTGATCCCAGCTGGAAGCGTCGGGAGCGTGGGGAC
TACTGGGCGCGAGGAGATGCGGTCAATCTGTCGATCGGACAAGGATTCTTCCTGGCGACGCCGCTGCAGTTGGCCGATGC
CTATGCGGCGCTCGCCAACGGGGGCACGCTCTGGCAGCCGTTCCTCGTCGAGCGAGTCGTCGCGATCGACGGCAGCGAGC
GTTTCCATGCCGAGCCGAAGGCGCGAGGAGCGCTCCCGTTGACACCAGAGCAGATCGACACGATCCGAGCCGCATTACGC
GATGTGGTCGCTCGGCCGAACGGGACAGCATGGAGTGCCTTCCAGGGTTTTACGGTTTCGGTGGCAGGCAAGACAGGTAC
GGCCGAGTCTGGCCAGGAGACGCCGCACGCCTGGTTCGTGGCGATCGCGCCAGCCGAGAATCCAGAACTCGTCCTGGTGG
TGATGGTGGAGCACGGCGGGGAAGGGTCGCGCGTCGCGGCACCGATCGCGCGGCAGATCCTCGAAACAGCGCAACAAGCC
GGGTATTTTCAGCGATAG

Upstream 100 bases:

>100_bases
CCACTGGTCGGGCGAACGGTGGCAGCGCTTGACGTCGCCCGGGTGAGCATGCAACGCTTCTCGGGCACGAAGCCGGACGG
CAGTTGCGTGAGGTGTGAGG

Downstream 100 bases:

>100_bases
GCCATGACGGGCTCGCCGTCTCCCACCGGGGGGTGCATGCTCGGCTCTTGCGGAGCGAGTACGAGGTCTGCACCGAGATC
GCGCAGGATCCGGCGTGCGA

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 725; Mature: 724

Protein sequence:

>725_residues
MPRATVRSSRRRSASGSLVAGLLIFAIVTGGIVAVALQFGDRQDTTTIGVADQSSASATLTRELAAPRTAREAAERFVRA
WEERDYRAMYALLSSAARQQIDEAQFIRRYEAISAEMGERSLQVTLGEALPGTMRFPIHVVRETTRVGRLEEDNAIPIVR
EGDAFRIDWTPSLIVADLGDGRVEWIPTVPQRGRLLDVKGRPLAQLGLVNKVGVVPGQIRDEHLLLERLSTLLNVPSETI
RQRYAGGQPDWFMPVATLPDPIDPGLLEQLAGIPGVVVRQWPERVYPLGPAAAHVTGYLSEVSAEELERRAADGYEPGDR
IGRAGIEAWAEDFLRGRRGGRLVIVGPDGRERRFLAEIPAQPAADVLTTIDTDLQEAAYRALGDRVGSVVAIDPRSGAIR
ALVSNPSFDPNRFILGLRTDEWQALNDEQRRPLIDRATQVGYPTGSTFKVVTMAAGLAYLGLRAESRFDCPPTFSLPGSS
VVWRDWNPRGQGQLTLHDALVQSCNTVFFQIGAQLDERDPNLLPQMARAFGFGSETGLTELPETAGLVPDPSWKRRERGD
YWARGDAVNLSIGQGFFLATPLQLADAYAALANGGTLWQPFLVERVVAIDGSERFHAEPKARGALPLTPEQIDTIRAALR
DVVARPNGTAWSAFQGFTVSVAGKTGTAESGQETPHAWFVAIAPAENPELVLVVMVEHGGEGSRVAAPIARQILETAQQA
GYFQR

Sequences:

>Translated_725_residues
MPRATVRSSRRRSASGSLVAGLLIFAIVTGGIVAVALQFGDRQDTTTIGVADQSSASATLTRELAAPRTAREAAERFVRA
WEERDYRAMYALLSSAARQQIDEAQFIRRYEAISAEMGERSLQVTLGEALPGTMRFPIHVVRETTRVGRLEEDNAIPIVR
EGDAFRIDWTPSLIVADLGDGRVEWIPTVPQRGRLLDVKGRPLAQLGLVNKVGVVPGQIRDEHLLLERLSTLLNVPSETI
RQRYAGGQPDWFMPVATLPDPIDPGLLEQLAGIPGVVVRQWPERVYPLGPAAAHVTGYLSEVSAEELERRAADGYEPGDR
IGRAGIEAWAEDFLRGRRGGRLVIVGPDGRERRFLAEIPAQPAADVLTTIDTDLQEAAYRALGDRVGSVVAIDPRSGAIR
ALVSNPSFDPNRFILGLRTDEWQALNDEQRRPLIDRATQVGYPTGSTFKVVTMAAGLAYLGLRAESRFDCPPTFSLPGSS
VVWRDWNPRGQGQLTLHDALVQSCNTVFFQIGAQLDERDPNLLPQMARAFGFGSETGLTELPETAGLVPDPSWKRRERGD
YWARGDAVNLSIGQGFFLATPLQLADAYAALANGGTLWQPFLVERVVAIDGSERFHAEPKARGALPLTPEQIDTIRAALR
DVVARPNGTAWSAFQGFTVSVAGKTGTAESGQETPHAWFVAIAPAENPELVLVVMVEHGGEGSRVAAPIARQILETAQQA
GYFQR
>Mature_724_residues
PRATVRSSRRRSASGSLVAGLLIFAIVTGGIVAVALQFGDRQDTTTIGVADQSSASATLTRELAAPRTAREAAERFVRAW
EERDYRAMYALLSSAARQQIDEAQFIRRYEAISAEMGERSLQVTLGEALPGTMRFPIHVVRETTRVGRLEEDNAIPIVRE
GDAFRIDWTPSLIVADLGDGRVEWIPTVPQRGRLLDVKGRPLAQLGLVNKVGVVPGQIRDEHLLLERLSTLLNVPSETIR
QRYAGGQPDWFMPVATLPDPIDPGLLEQLAGIPGVVVRQWPERVYPLGPAAAHVTGYLSEVSAEELERRAADGYEPGDRI
GRAGIEAWAEDFLRGRRGGRLVIVGPDGRERRFLAEIPAQPAADVLTTIDTDLQEAAYRALGDRVGSVVAIDPRSGAIRA
LVSNPSFDPNRFILGLRTDEWQALNDEQRRPLIDRATQVGYPTGSTFKVVTMAAGLAYLGLRAESRFDCPPTFSLPGSSV
VWRDWNPRGQGQLTLHDALVQSCNTVFFQIGAQLDERDPNLLPQMARAFGFGSETGLTELPETAGLVPDPSWKRRERGDY
WARGDAVNLSIGQGFFLATPLQLADAYAALANGGTLWQPFLVERVVAIDGSERFHAEPKARGALPLTPEQIDTIRAALRD
VVARPNGTAWSAFQGFTVSVAGKTGTAESGQETPHAWFVAIAPAENPELVLVVMVEHGGEGSRVAAPIARQILETAQQAG
YFQR

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=561, Percent_Identity=31.5508021390374, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI1786272, Length=580, Percent_Identity=25.3448275862069, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 79041; Mature: 78910

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRATVRSSRRRSASGSLVAGLLIFAIVTGGIVAVALQFGDRQDTTTIGVADQSSASATL
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCEEEEECCCCCCHHHH
TRELAAPRTAREAAERFVRAWEERDYRAMYALLSSAARQQIDEAQFIRRYEAISAEMGER
HHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SLQVTLGEALPGTMRFPIHVVRETTRVGRLEEDNAIPIVREGDAFRIDWTPSLIVADLGD
EEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCCEEEEECCC
GRVEWIPTVPQRGRLLDVKGRPLAQLGLVNKVGVVPGQIRDEHLLLERLSTLLNVPSETI
CCEEEEECCCCCCCEEECCCCCHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
RQRYAGGQPDWFMPVATLPDPIDPGLLEQLAGIPGVVVRQWPERVYPLGPAAAHVTGYLS
HHHHCCCCCCCEEECCCCCCCCCHHHHHHHCCCCCHHHHHCHHHHCCCCCHHHHHHHHHH
EVSAEELERRAADGYEPGDRIGRAGIEAWAEDFLRGRRGGRLVIVGPDGRERRFLAEIPA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCC
QPAADVLTTIDTDLQEAAYRALGDRVGSVVAIDPRSGAIRALVSNPSFDPNRFILGLRTD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCCCCCEEEEEECC
EWQALNDEQRRPLIDRATQVGYPTGSTFKVVTMAAGLAYLGLRAESRFDCPPTFSLPGSS
CCCCCCCHHCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
VVWRDWNPRGQGQLTLHDALVQSCNTVFFQIGAQLDERDPNLLPQMARAFGFGSETGLTE
EEEECCCCCCCCCEEHHHHHHHHHHHEEHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
LPETAGLVPDPSWKRRERGDYWARGDAVNLSIGQGFFLATPLQLADAYAALANGGTLWQP
CCHHCCCCCCCCHHHHCCCCCEECCCEEEEEECCCEEEECHHHHHHHHHHHHCCCCCHHH
FLVERVVAIDGSERFHAEPKARGALPLTPEQIDTIRAALRDVVARPNGTAWSAFQGFTVS
HHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEE
VAGKTGTAESGQETPHAWFVAIAPAENPELVLVVMVEHGGEGSRVAAPIARQILETAQQA
EECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHC
GYFQR
CCCCC
>Mature Secondary Structure 
PRATVRSSRRRSASGSLVAGLLIFAIVTGGIVAVALQFGDRQDTTTIGVADQSSASATL
CCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCEEEEECCCCCCHHHH
TRELAAPRTAREAAERFVRAWEERDYRAMYALLSSAARQQIDEAQFIRRYEAISAEMGER
HHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SLQVTLGEALPGTMRFPIHVVRETTRVGRLEEDNAIPIVREGDAFRIDWTPSLIVADLGD
EEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCCEEEEECCC
GRVEWIPTVPQRGRLLDVKGRPLAQLGLVNKVGVVPGQIRDEHLLLERLSTLLNVPSETI
CCEEEEECCCCCCCEEECCCCCHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
RQRYAGGQPDWFMPVATLPDPIDPGLLEQLAGIPGVVVRQWPERVYPLGPAAAHVTGYLS
HHHHCCCCCCCEEECCCCCCCCCHHHHHHHCCCCCHHHHHCHHHHCCCCCHHHHHHHHHH
EVSAEELERRAADGYEPGDRIGRAGIEAWAEDFLRGRRGGRLVIVGPDGRERRFLAEIPA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCC
QPAADVLTTIDTDLQEAAYRALGDRVGSVVAIDPRSGAIRALVSNPSFDPNRFILGLRTD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECCCCCCCCCEEEEEECC
EWQALNDEQRRPLIDRATQVGYPTGSTFKVVTMAAGLAYLGLRAESRFDCPPTFSLPGSS
CCCCCCCHHCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
VVWRDWNPRGQGQLTLHDALVQSCNTVFFQIGAQLDERDPNLLPQMARAFGFGSETGLTE
EEEECCCCCCCCCEEHHHHHHHHHHHEEHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
LPETAGLVPDPSWKRRERGDYWARGDAVNLSIGQGFFLATPLQLADAYAALANGGTLWQP
CCHHCCCCCCCCHHHHCCCCCEECCCEEEEEECCCEEEECHHHHHHHHHHHHCCCCCHHH
FLVERVVAIDGSERFHAEPKARGALPLTPEQIDTIRAALRDVVARPNGTAWSAFQGFTVS
HHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEE
VAGKTGTAESGQETPHAWFVAIAPAENPELVLVVMVEHGGEGSRVAAPIARQILETAQQA
EECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHC
GYFQR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 8930920 [H]