| Definition | Thermomicrobium roseum DSM 5159 chromosome, complete genome. |
|---|---|
| Accession | NC_011959 |
| Length | 2,003,006 |
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The map label for this gene is metE [H]
Identifier: 221633329
GI number: 221633329
Start: 1341376
End: 1343667
Strand: Reverse
Name: metE [H]
Synonym: trd_1349
Alternate gene names: 221633329
Gene position: 1343667-1341376 (Counterclockwise)
Preceding gene: 221633330
Following gene: 221633328
Centisome position: 67.08
GC content: 64.4
Gene sequence:
>2292_bases ATGGCCCAGAGCTCGGTCCTCGGCTATCCCCGAATCGGCAAGCAGCGTGAACTGAAGCGGGCCCTCGAGCGTTACTGGGC CGGTGAGGCTGATGCAGCGGCCTTGCAGGAGACGGCTCGAGCAGTCCGCGAAGCGGCGTGGCGTGTGCAGTTCCAGGCCG GGATCGATCAGATTCCCTGTAACGATTTCAGCCTCTACGACCATGTCCTGGATACGATCTGTCTGGTCGGGCTCGTGCCG TCCCGGTTCGGCTCGGCCGAGCCAGAAGTCAGTCTGCCGACCTACTTCGCCATGGCGCGGGGGGCACCGGGTGTTCCAGC GCTCGAGTTGACGAAGTGGTTCGATACCAACTACCACTATCTCGTACCGGAATTCGATGCGCCTGGTCCGCGTCTGGCCA GCGCCAAGCCCTTCCTCGAGTTCGCCGAGGCGACGTCGCTCTTGGGACGACCGGCCAAGCCGGTTCTGCTGGGCCCGATT TCGCTTTTGCTCCTCGGCAAGTATCACCAGCGGCCGTTGCGCGAGCACCTGGAGCAATTGCTCCCGATCTACCAGGAGGT CTTGGAGCGGCTCGCACGGGCTGGTGCCGAGTGGGTCCAGATGGACGAGCCGGTTCTCGTGCAGGATCGCAGCGACGAAG AGTTGCAGCTGCTCCGGGCAGCCTATCGCGTGCTCGGCGAAGCGAGCGCTCGTCCGAAGCTGATGCTGCAGACCTACTAT GGCAGTCTCGACGATGCGAGTTGGGACGTCGTCATGAGTCTGCCGATCGAAGGGATCGGTCTGGACTTCGTGCGGGACGA CGGTAACCGAGCTTTGCTCAACCGGCACGGCTTCCCCCAGGGGAAGGTGATCGGTGCGGGAGTCGTGAATGGGCGCAACA TCTGGCGAACGAATCTTGTGGAAGCTCTCGAGAAGCTGGAAGAGATCGCCGAGCATGTGGAGCCGGAGCGTATCTGGGTC CAGCCGTCCTGCACGCTCTTGCATCTCCCGCACGACGTGCGACTGGAAACCAAGCTCCCGGCTGCAGTGCGCGCATTGCT GGCGTTCGCTGAGCAACGACTGGAAGAGTTGGTGATCTTGACGCGTGCACTCAACGAGGGACGTGCAGCCATTCTGGAAG AGCTCGAGCAGAACGCGGAGGCGTTTCGCCTGGCGCTGGACGAGGTTCGGGTCTACCGGCGCGACGTGCGCGAGCGGCTG GCTGCGCTCACCGAGGACGACTTCCGGCGCCAGTCGCCGTACGAGATGCGCGAACCGCTCCAGCGGGCGCGCTTCCACCT TCCCCTCTTTCCGACGACGACGATCGGCAGCTTTCCGCAAACGCCGGACGTCCGCCAGGCCCGTGCACGCTGGCGTGCCG GCAAGCTCTCGGATGAGGACTATTGGGGGTTCATCCGCGAGCGGATCGCCGAGGTCATCCGGCGTCAGGAGGAGATCGGG CTGGATGTCCTGGTGCACGGCGAGTACGAGCGTGCCGACATGGTGGAGTACTTCGCCGAGCAACTGGAAGGATTCGTCCT GACCGAACATGGCTGGGTGCAGTCGTACGGGAGCCGCGTGGTCAGACCGCCGATCATCTTCGGTGATGTGGCGCGACCAC GACCGATGACAGTTGAAGTGAGTCGGTGTGCGCAGTCGCTGACCGAGAAACCGGTCAAAGGCATGCTGACCGGTCCGGTG ACGATCCTGAACTGGTCGTTCGTCCGGGAAGATATCCCGCGACGCGAGGTCGCGTACCAGATCGCGCTGGCGCTGCGCGA CGAGGTTCGTGACCTGGAGGCTGCCGGGATCAGCATGATCCAGATCGACGAGCCGGCGCTCCGTGAGGGGCTTCCCTTGC GTCGAGCAGACTGGCCGGAGTACCTCGAGTGGGCCGTGCGCGCCTTCCGCCTGGCGAGTTCGGGCGTTCGTGACGACACG CAAATCCACTCGCACATGTGCTACAGCGAGTTCAACGACATCATCGAGGCGATCGACGCGCTCGACGCGGACGTCATCTC GATCGAGAACAGTCGGAGTCGGGGAGAGCTCCTGCAGGCGTTCCAGGAGTACCGCTACCCGCGGCAGATTGGTCCGGGCG TCTACGACGTCCACTCCGCGCGGATCCCGAGCGTCGAGGAGATGCTCGAGCTGCTGGAGCGTGCGCTCGCATTCCTGCGA CCGGAGCAGCTGTGGGTCAATCCGGACTGCGGACTCAAGACCCGGCGTCCGGAGGAGGTTTGGCCATCGCTCAGCAACAT GGTCGAAGCCGCGCGCCGACTCCGCGCGCGCTTCACAGCGCAGGGCCAGTGA
Upstream 100 bases:
>100_bases TGCCCTTTGACGATGCGCTCATCGCGTGTTATCGTCGCCGAGCGAGTCGTGATGAGCGATCGCAAGCCTGAGTGAACCGA ACAGAGGAGAGAGGGAGCGC
Downstream 100 bases:
>100_bases AGCTGAGGAACTGCTCACGGGGGCGATGATGGGCCAGGCGGTCGATACGCGTTCGCTTCCCGTCATGCCGTTCGGCCCAT TCACCTTGCGGCCGTTCGCC
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 763; Mature: 762
Protein sequence:
>763_residues MAQSSVLGYPRIGKQRELKRALERYWAGEADAAALQETARAVREAAWRVQFQAGIDQIPCNDFSLYDHVLDTICLVGLVP SRFGSAEPEVSLPTYFAMARGAPGVPALELTKWFDTNYHYLVPEFDAPGPRLASAKPFLEFAEATSLLGRPAKPVLLGPI SLLLLGKYHQRPLREHLEQLLPIYQEVLERLARAGAEWVQMDEPVLVQDRSDEELQLLRAAYRVLGEASARPKLMLQTYY GSLDDASWDVVMSLPIEGIGLDFVRDDGNRALLNRHGFPQGKVIGAGVVNGRNIWRTNLVEALEKLEEIAEHVEPERIWV QPSCTLLHLPHDVRLETKLPAAVRALLAFAEQRLEELVILTRALNEGRAAILEELEQNAEAFRLALDEVRVYRRDVRERL AALTEDDFRRQSPYEMREPLQRARFHLPLFPTTTIGSFPQTPDVRQARARWRAGKLSDEDYWGFIRERIAEVIRRQEEIG LDVLVHGEYERADMVEYFAEQLEGFVLTEHGWVQSYGSRVVRPPIIFGDVARPRPMTVEVSRCAQSLTEKPVKGMLTGPV TILNWSFVREDIPRREVAYQIALALRDEVRDLEAAGISMIQIDEPALREGLPLRRADWPEYLEWAVRAFRLASSGVRDDT QIHSHMCYSEFNDIIEAIDALDADVISIENSRSRGELLQAFQEYRYPRQIGPGVYDVHSARIPSVEEMLELLERALAFLR PEQLWVNPDCGLKTRRPEEVWPSLSNMVEAARRLRARFTAQGQ
Sequences:
>Translated_763_residues MAQSSVLGYPRIGKQRELKRALERYWAGEADAAALQETARAVREAAWRVQFQAGIDQIPCNDFSLYDHVLDTICLVGLVP SRFGSAEPEVSLPTYFAMARGAPGVPALELTKWFDTNYHYLVPEFDAPGPRLASAKPFLEFAEATSLLGRPAKPVLLGPI SLLLLGKYHQRPLREHLEQLLPIYQEVLERLARAGAEWVQMDEPVLVQDRSDEELQLLRAAYRVLGEASARPKLMLQTYY GSLDDASWDVVMSLPIEGIGLDFVRDDGNRALLNRHGFPQGKVIGAGVVNGRNIWRTNLVEALEKLEEIAEHVEPERIWV QPSCTLLHLPHDVRLETKLPAAVRALLAFAEQRLEELVILTRALNEGRAAILEELEQNAEAFRLALDEVRVYRRDVRERL AALTEDDFRRQSPYEMREPLQRARFHLPLFPTTTIGSFPQTPDVRQARARWRAGKLSDEDYWGFIRERIAEVIRRQEEIG LDVLVHGEYERADMVEYFAEQLEGFVLTEHGWVQSYGSRVVRPPIIFGDVARPRPMTVEVSRCAQSLTEKPVKGMLTGPV TILNWSFVREDIPRREVAYQIALALRDEVRDLEAAGISMIQIDEPALREGLPLRRADWPEYLEWAVRAFRLASSGVRDDT QIHSHMCYSEFNDIIEAIDALDADVISIENSRSRGELLQAFQEYRYPRQIGPGVYDVHSARIPSVEEMLELLERALAFLR PEQLWVNPDCGLKTRRPEEVWPSLSNMVEAARRLRARFTAQGQ >Mature_762_residues AQSSVLGYPRIGKQRELKRALERYWAGEADAAALQETARAVREAAWRVQFQAGIDQIPCNDFSLYDHVLDTICLVGLVPS RFGSAEPEVSLPTYFAMARGAPGVPALELTKWFDTNYHYLVPEFDAPGPRLASAKPFLEFAEATSLLGRPAKPVLLGPIS LLLLGKYHQRPLREHLEQLLPIYQEVLERLARAGAEWVQMDEPVLVQDRSDEELQLLRAAYRVLGEASARPKLMLQTYYG SLDDASWDVVMSLPIEGIGLDFVRDDGNRALLNRHGFPQGKVIGAGVVNGRNIWRTNLVEALEKLEEIAEHVEPERIWVQ PSCTLLHLPHDVRLETKLPAAVRALLAFAEQRLEELVILTRALNEGRAAILEELEQNAEAFRLALDEVRVYRRDVRERLA ALTEDDFRRQSPYEMREPLQRARFHLPLFPTTTIGSFPQTPDVRQARARWRAGKLSDEDYWGFIRERIAEVIRRQEEIGL DVLVHGEYERADMVEYFAEQLEGFVLTEHGWVQSYGSRVVRPPIIFGDVARPRPMTVEVSRCAQSLTEKPVKGMLTGPVT ILNWSFVREDIPRREVAYQIALALRDEVRDLEAAGISMIQIDEPALREGLPLRRADWPEYLEWAVRAFRLASSGVRDDTQ IHSHMCYSEFNDIIEAIDALDADVISIENSRSRGELLQAFQEYRYPRQIGPGVYDVHSARIPSVEEMLELLERALAFLRP EQLWVNPDCGLKTRRPEEVWPSLSNMVEAARRLRARFTAQGQ
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=758, Percent_Identity=50.7915567282322, Blast_Score=725, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6320936, Length=780, Percent_Identity=46.5384615384615, Blast_Score=658, Evalue=0.0,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 86985; Mature: 86854
Theoretical pI: Translated: 5.09; Mature: 5.09
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 2.5 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAQSSVLGYPRIGKQRELKRALERYWAGEADAAALQETARAVREAAWRVQFQAGIDQIPC CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC NDFSLYDHVLDTICLVGLVPSRFGSAEPEVSLPTYFAMARGAPGVPALELTKWFDTNYHY CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCEE LVPEFDAPGPRLASAKPFLEFAEATSLLGRPAKPVLLGPISLLLLGKYHQRPLREHLEQL EECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH LPIYQEVLERLARAGAEWVQMDEPVLVQDRSDEELQLLRAAYRVLGEASARPKLMLQTYY HHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHC GSLDDASWDVVMSLPIEGIGLDFVRDDGNRALLNRHGFPQGKVIGAGVVNGRNIWRTNLV CCCCCCCCCEEEECCCCCCCCEEEECCCCEEHHHCCCCCCCCEEEEEECCCCHHHHHHHH EALEKLEEIAEHVEPERIWVQPSCTLLHLPHDVRLETKLPAAVRALLAFAEQRLEELVIL HHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHH TRALNEGRAAILEELEQNAEAFRLALDEVRVYRRDVRERLAALTEDDFRRQSPYEMREPL HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHH QRARFHLPLFPTTTIGSFPQTPDVRQARARWRAGKLSDEDYWGFIRERIAEVIRRQEEIG HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC LDVLVHGEYERADMVEYFAEQLEGFVLTEHGWVQSYGSRVVRPPIIFGDVARPRPMTVEV CEEEEECCCCHHHHHHHHHHHHCCEEEECCCHHHHHCCCCCCCCEEECCCCCCCCCEEEH SRCAQSLTEKPVKGMLTGPVTILNWSFVREDIPRREVAYQIALALRDEVRDLEAAGISMI HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE QIDEPALREGLPLRRADWPEYLEWAVRAFRLASSGVRDDTQIHSHMCYSEFNDIIEAIDA EECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH LDADVISIENSRSRGELLQAFQEYRYPRQIGPGVYDVHSARIPSVEEMLELLERALAFLR CCCCEEEECCCCCHHHHHHHHHHCCCCHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHC PEQLWVNPDCGLKTRRPEEVWPSLSNMVEAARRLRARFTAQGQ CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC >Mature Secondary Structure AQSSVLGYPRIGKQRELKRALERYWAGEADAAALQETARAVREAAWRVQFQAGIDQIPC CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC NDFSLYDHVLDTICLVGLVPSRFGSAEPEVSLPTYFAMARGAPGVPALELTKWFDTNYHY CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCEE LVPEFDAPGPRLASAKPFLEFAEATSLLGRPAKPVLLGPISLLLLGKYHQRPLREHLEQL EECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH LPIYQEVLERLARAGAEWVQMDEPVLVQDRSDEELQLLRAAYRVLGEASARPKLMLQTYY HHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHC GSLDDASWDVVMSLPIEGIGLDFVRDDGNRALLNRHGFPQGKVIGAGVVNGRNIWRTNLV CCCCCCCCCEEEECCCCCCCCEEEECCCCEEHHHCCCCCCCCEEEEEECCCCHHHHHHHH EALEKLEEIAEHVEPERIWVQPSCTLLHLPHDVRLETKLPAAVRALLAFAEQRLEELVIL HHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHH TRALNEGRAAILEELEQNAEAFRLALDEVRVYRRDVRERLAALTEDDFRRQSPYEMREPL HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHH QRARFHLPLFPTTTIGSFPQTPDVRQARARWRAGKLSDEDYWGFIRERIAEVIRRQEEIG HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC LDVLVHGEYERADMVEYFAEQLEGFVLTEHGWVQSYGSRVVRPPIIFGDVARPRPMTVEV CEEEEECCCCHHHHHHHHHHHHCCEEEECCCHHHHHCCCCCCCCEEECCCCCCCCCEEEH SRCAQSLTEKPVKGMLTGPVTILNWSFVREDIPRREVAYQIALALRDEVRDLEAAGISMI HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE QIDEPALREGLPLRRADWPEYLEWAVRAFRLASSGVRDDTQIHSHMCYSEFNDIIEAIDA EECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH LDADVISIENSRSRGELLQAFQEYRYPRQIGPGVYDVHSARIPSVEEMLELLERALAFLR CCCCEEEECCCCCHHHHHHHHHHCCCCHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHC PEQLWVNPDCGLKTRRPEEVWPSLSNMVEAARRLRARFTAQGQ CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA