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The map label for this gene is purN [H]

Identifier: 221633167

GI number: 221633167

Start: 1172229

End: 1172852

Strand: Reverse

Name: purN [H]

Synonym: trd_1183

Alternate gene names: 221633167

Gene position: 1172852-1172229 (Counterclockwise)

Preceding gene: 221633170

Following gene: 221633166

Centisome position: 58.55

GC content: 61.7

Gene sequence:

>624_bases
GTGCGGCAACTGCGGATCGCTGTCCTGATTTCGGGAAGCGGGCGGACACTGGCCAATCTACTCGCGGTGCAAGGCCGGGG
TGAGCTGCCGGGGCGCATCGAACTCGTCGTCAGTAACCGTCCCGATGTTGCTGGCAACGACATCGCTCGTGCTGCTGGGG
TGCCACTCGCGATCATCCCCTCCAGACGTGTGCCGGAGTCTGCCTTCGCTGAGCAGGTGTATCGTCTCCTGGACCAGCAC
GCGATCGATTTGGTGCTCATGGCTGGATTTCTCCGGCATTTGCCGGTGCGTGCCGACTATCGATGGCGCATCATGAACAT
TCACCCGTCCCTTCTCCCGCTGTTCGGGGGGCGTGGGATGTACGGCGAGCGTGTTCACCGTGCTGTCCTGGACAGCGGCG
TGAAGGTGAGCGGCTGCACGGTGCATTTCGTCACCGATGAACTCGATGCAGGACCGATCATCCTGCAAGCCTGCGTGCCG
GTCTTGGATGACGACACACCGGAGACACTCGCAGCGCGCGTTTTCGCCGAAGAATGCCGTCTCTATCCGGAGGCTGTGCG
GCTGTATGCTGCTGGTCGGTTGCGGGTGGAAGGTCGACGAGTGCGTATTCTTTCTTCTCCATGA

Upstream 100 bases:

>100_bases
ACGTGGCCGATACCACGATTGTACCGAAGGTACGTGACATCGGCGGGGCGCTCTCACATACTACGGCTGAGAACGGAGCA
CGTGAGGTGAAGGAGCCAGC

Downstream 100 bases:

>100_bases
GGAGGGACGACTGTGAACAGTCCGTCGTATCGAACGCTCCTGGATACCCCATGCCTGGTCGTCGATCTCGATCGTTTGGA
GCGCAATCTCGCCGAGATGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MRQLRIAVLISGSGRTLANLLAVQGRGELPGRIELVVSNRPDVAGNDIARAAGVPLAIIPSRRVPESAFAEQVYRLLDQH
AIDLVLMAGFLRHLPVRADYRWRIMNIHPSLLPLFGGRGMYGERVHRAVLDSGVKVSGCTVHFVTDELDAGPIILQACVP
VLDDDTPETLAARVFAEECRLYPEAVRLYAAGRLRVEGRRVRILSSP

Sequences:

>Translated_207_residues
MRQLRIAVLISGSGRTLANLLAVQGRGELPGRIELVVSNRPDVAGNDIARAAGVPLAIIPSRRVPESAFAEQVYRLLDQH
AIDLVLMAGFLRHLPVRADYRWRIMNIHPSLLPLFGGRGMYGERVHRAVLDSGVKVSGCTVHFVTDELDAGPIILQACVP
VLDDDTPETLAARVFAEECRLYPEAVRLYAAGRLRVEGRRVRILSSP
>Mature_207_residues
MRQLRIAVLISGSGRTLANLLAVQGRGELPGRIELVVSNRPDVAGNDIARAAGVPLAIIPSRRVPESAFAEQVYRLLDQH
AIDLVLMAGFLRHLPVRADYRWRIMNIHPSLLPLFGGRGMYGERVHRAVLDSGVKVSGCTVHFVTDELDAGPIILQACVP
VLDDDTPETLAARVFAEECRLYPEAVRLYAAGRLRVEGRRVRILSSP

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=198, Percent_Identity=39.3939393939394, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI209869995, Length=198, Percent_Identity=39.3939393939394, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI209869993, Length=198, Percent_Identity=39.3939393939394, Blast_Score=138, Evalue=4e-33,
Organism=Escherichia coli, GI1788846, Length=197, Percent_Identity=38.0710659898477, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1787483, Length=202, Percent_Identity=27.7227722772277, Blast_Score=72, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=194, Percent_Identity=34.5360824742268, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582400, Length=200, Percent_Identity=37, Blast_Score=117, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22746; Mature: 22746

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLRIAVLISGSGRTLANLLAVQGRGELPGRIELVVSNRPDVAGNDIARAAGVPLAIIP
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCEEEEC
SRRVPESAFAEQVYRLLDQHAIDLVLMAGFLRHLPVRADYRWRIMNIHPSLLPLFGGRGM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHCCCCCC
YGERVHRAVLDSGVKVSGCTVHFVTDELDAGPIILQACVPVLDDDTPETLAARVFAEECR
CHHHHHHHHHHCCCEECCEEEEEEECCCCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHH
LYPEAVRLYAAGRLRVEGRRVRILSSP
CCHHHHHHHHCCEEEECCEEEEEEECC
>Mature Secondary Structure
MRQLRIAVLISGSGRTLANLLAVQGRGELPGRIELVVSNRPDVAGNDIARAAGVPLAIIP
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCEEEEC
SRRVPESAFAEQVYRLLDQHAIDLVLMAGFLRHLPVRADYRWRIMNIHPSLLPLFGGRGM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHCCCCCC
YGERVHRAVLDSGVKVSGCTVHFVTDELDAGPIILQACVPVLDDDTPETLAARVFAEECR
CHHHHHHHHHHCCCEECCEEEEEEECCCCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHH
LYPEAVRLYAAGRLRVEGRRVRILSSP
CCHHHHHHHHCCEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]