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The map label for this gene is ubiE [H]

Identifier: 221633053

GI number: 221633053

Start: 1043835

End: 1044704

Strand: Reverse

Name: ubiE [H]

Synonym: trd_1069

Alternate gene names: 221633053

Gene position: 1044704-1043835 (Counterclockwise)

Preceding gene: 221633054

Following gene: 221633052

Centisome position: 52.16

GC content: 63.1

Gene sequence:

>870_bases
ATGGTGAAAGAGGTCGGCCGGTACCGGGTCGATGATCCCCGGACGATTCAAGGCCGGGACGACGTTTCGCCGTGCGGGTG
GCATCTCGGCGCCCTGCGGTTGCGGTTCGCATTGGAAGCCCTCGCGAGCGTAAACGGTCGGGTACTGGTTCCTGGATGTG
GAGCGGGACGCTACGTGCGGGCGCTTCACCGGGAGCGGCCGGATCTCTGGGTCGTCGGTGGTGATCTCAGTCGCCGGGCG
ATCCGCGAGGCGCGCGAGCGTGATCCGAACGGGCCGTACGTCGTCCTCGATGCCTGTCGTCTTCCGTTCCGTTCCGGAAG
CTTCGATGCTGTCGTGTTTCTGGACGTGTTGGAGCATGTACCGGATCCAGCGAGGATGATCGCCGAGTGTGCGCGTGTTT
TGCGCCCAGGCGGTGTACTGCATGCGTTCGTCCCTTTGGAAGCACAGCCAGCGACGATCTACTGGTGGCTGCGAGCCAGC
CAGCGTTGGCCTATCCTTCGCTGGAAACGCGATCACGTCGGGCATATCCAGCGCTTTTCCGATCTGGACATCATACGCCT
GTTGGCTCGTATCGGTTTCGAAATCCAATTCCTGCGCTACAGTTTTCATCTCGTCGGTCAGCTGCACGATCTGATCGACT
ACTGGCAGCGGGAGCGCGTGGCCGGTGCGCCTGGCTGGCTCCCGGTTCCGGTGGTGCGACTGCTGACTCGTCTCGTCTTT
CTGGTGACGTGGCGGCTCGCTGCGGTGGAAGATCTCCTTTGCCGGAGGCGTACTCTGGCTGCTGGGCTGCATGTCACAGC
GTCCAAGCCGAAGATGCTGGAGGGTGCTCGGGCTGGTGAAGCACAGGTGGTCGGCCGAGCGATGCCGTGA

Upstream 100 bases:

>100_bases
CGGGAGTGAGTACGCGGGAAGATCTCACCATGACCGAATGGCTTCCCGATCTGGTTTTGAGCGATTTGCGTGAGCTGCTC
GCGGCGGTACGGAGCCTTCG

Downstream 100 bases:

>100_bases
GGAGGTTCTCGTGGACTGGGGTCCAATCATCGATGGGCACAGCGATTTTGTCCTCTCTCTCCTCGAGACCGGTCGAGACT
TTCTCGTCGAAAGTCACGAA

Product: putative methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MVKEVGRYRVDDPRTIQGRDDVSPCGWHLGALRLRFALEALASVNGRVLVPGCGAGRYVRALHRERPDLWVVGGDLSRRA
IREARERDPNGPYVVLDACRLPFRSGSFDAVVFLDVLEHVPDPARMIAECARVLRPGGVLHAFVPLEAQPATIYWWLRAS
QRWPILRWKRDHVGHIQRFSDLDIIRLLARIGFEIQFLRYSFHLVGQLHDLIDYWQRERVAGAPGWLPVPVVRLLTRLVF
LVTWRLAAVEDLLCRRRTLAAGLHVTASKPKMLEGARAGEAQVVGRAMP

Sequences:

>Translated_289_residues
MVKEVGRYRVDDPRTIQGRDDVSPCGWHLGALRLRFALEALASVNGRVLVPGCGAGRYVRALHRERPDLWVVGGDLSRRA
IREARERDPNGPYVVLDACRLPFRSGSFDAVVFLDVLEHVPDPARMIAECARVLRPGGVLHAFVPLEAQPATIYWWLRAS
QRWPILRWKRDHVGHIQRFSDLDIIRLLARIGFEIQFLRYSFHLVGQLHDLIDYWQRERVAGAPGWLPVPVVRLLTRLVF
LVTWRLAAVEDLLCRRRTLAAGLHVTASKPKMLEGARAGEAQVVGRAMP
>Mature_289_residues
MVKEVGRYRVDDPRTIQGRDDVSPCGWHLGALRLRFALEALASVNGRVLVPGCGAGRYVRALHRERPDLWVVGGDLSRRA
IREARERDPNGPYVVLDACRLPFRSGSFDAVVFLDVLEHVPDPARMIAECARVLRPGGVLHAFVPLEAQPATIYWWLRAS
QRWPILRWKRDHVGHIQRFSDLDIIRLLARIGFEIQFLRYSFHLVGQLHDLIDYWQRERVAGAPGWLPVPVVRLLTRLVF
LVTWRLAAVEDLLCRRRTLAAGLHVTASKPKMLEGARAGEAQVVGRAMP

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 32816; Mature: 32816

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKEVGRYRVDDPRTIQGRDDVSPCGWHLGALRLRFALEALASVNGRVLVPGCGAGRYVR
CCCCCCCEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
ALHRERPDLWVVGGDLSRRAIREARERDPNGPYVVLDACRLPFRSGSFDAVVFLDVLEHV
HHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHC
PDPARMIAECARVLRPGGVLHAFVPLEAQPATIYWWLRASQRWPILRWKRDHVGHIQRFS
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCCCCCEEECCHHHHHHHHHC
DLDIIRLLARIGFEIQFLRYSFHLVGQLHDLIDYWQRERVAGAPGWLPVPVVRLLTRLVF
CHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LVTWRLAAVEDLLCRRRTLAAGLHVTASKPKMLEGARAGEAQVVGRAMP
HHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHCCCCCCCHHHCCCCCC
>Mature Secondary Structure
MVKEVGRYRVDDPRTIQGRDDVSPCGWHLGALRLRFALEALASVNGRVLVPGCGAGRYVR
CCCCCCCEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHH
ALHRERPDLWVVGGDLSRRAIREARERDPNGPYVVLDACRLPFRSGSFDAVVFLDVLEHV
HHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHC
PDPARMIAECARVLRPGGVLHAFVPLEAQPATIYWWLRASQRWPILRWKRDHVGHIQRFS
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCCCCCEEECCHHHHHHHHHC
DLDIIRLLARIGFEIQFLRYSFHLVGQLHDLIDYWQRERVAGAPGWLPVPVVRLLTRLVF
CHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LVTWRLAAVEDLLCRRRTLAAGLHVTASKPKMLEGARAGEAQVVGRAMP
HHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA