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The map label for this gene is rihA [H]

Identifier: 221633014

GI number: 221633014

Start: 993689

End: 994618

Strand: Reverse

Name: rihA [H]

Synonym: trd_1029

Alternate gene names: 221633014

Gene position: 994618-993689 (Counterclockwise)

Preceding gene: 221633016

Following gene: 221633013

Centisome position: 49.66

GC content: 63.87

Gene sequence:

>930_bases
GTGAAGACGATGCAGCCCGTCATCGTGGATGTCGATACCGGAGTCGATGATGCGCTGGCACTCGCGGTGGCGCTTGCGCA
GCCGGAACTGCGCCTGATCGCGGTGACCACAGTAGCTGGAAACGTTGATGTCGGGCAAGCGACGCGGAACACGCGCCTCG
TCCTCGATTGGCTGGGGGCGCGCTCGATTCCGGTTGCGAAAGGTGCAGCCAGTCCACTTCTCCGTAAACATCGAGATGCG
CGCGCCTATCATAGTCCAGACGGTCTGGGTGGTGCGGTGCTGCCAGTGGAGCGGGGTGGACCGATACTCGACACGAGTGC
GCCGGAAGTCATCGTGCGAGCTGCACGCCGCTATGCTGGAAAACTTTGCCTGGTTTGTCTCGCTCCGTTGACGAACCTCG
CCATCGCACTGCGGCTCGAACCGGACCTTCCCTTTTTGATCGATCGAGTGATCGTGATGGGCGGCGCCTTCGCTGTGGCG
GGGAACGTGACGCCGGCTGCCGAATTCAACGTCTGGAGCGATCCGGAAGCGGCGTCGCTGGTGGCTCAGGCGGGCTTCCG
GATTCGATGGGTGGGCTTGGATGTCACCGAGTTGGTTCGGCTCGAACGCTCCCGGTGGGAGCGATTGGGGAACGCCACCG
ATCCGCGATCGATACTCGCTCGCGAAGTTGGCCGGTGGGCATTCGAGCAACGTGGGCTGACGTCGTTTGCGCTGCATGAT
CCTCTGGCGGTCGTGGCTGCTGTGCGGGAAGAGTTCATCGACTGGGCGCCCGGGCACGTCATCGTCCGGACGGAGACCGA
CGAGGTGGCGGGGAAAACGGTTTACCAGAGCGATCCCGATGGACGAGCGCGGGTTGCGCACCGGGTGAACAGCGACGGCG
CGCAAGCCTTCTTGGAGCGGGCGCTCGGTCTCGTTGGAAAAGGGGGGTGA

Upstream 100 bases:

>100_bases
CGATCGGGTGCGTCGGATGACCTGCACGCCAGGCTCCTCCATCTTTCGACCGCGGCGAGTATACCTAGCTCGGAGAGATC
GATCGCAATCGAGCCGAAAG

Downstream 100 bases:

>100_bases
CGTGGCCATCATCTGATGCGCCATAATAGGCGTATCAGGAAGCGAACCGGGCGAGAGCCCGGCGACTGGAGGCGAGAGCC
GTGAGCGGTGAGACGCTACC

Product: inosine-uridine preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKTMQPVIVDVDTGVDDALALAVALAQPELRLIAVTTVAGNVDVGQATRNTRLVLDWLGARSIPVAKGAASPLLRKHRDA
RAYHSPDGLGGAVLPVERGGPILDTSAPEVIVRAARRYAGKLCLVCLAPLTNLAIALRLEPDLPFLIDRVIVMGGAFAVA
GNVTPAAEFNVWSDPEAASLVAQAGFRIRWVGLDVTELVRLERSRWERLGNATDPRSILAREVGRWAFEQRGLTSFALHD
PLAVVAAVREEFIDWAPGHVIVRTETDEVAGKTVYQSDPDGRARVAHRVNSDGAQAFLERALGLVGKGG

Sequences:

>Translated_309_residues
MKTMQPVIVDVDTGVDDALALAVALAQPELRLIAVTTVAGNVDVGQATRNTRLVLDWLGARSIPVAKGAASPLLRKHRDA
RAYHSPDGLGGAVLPVERGGPILDTSAPEVIVRAARRYAGKLCLVCLAPLTNLAIALRLEPDLPFLIDRVIVMGGAFAVA
GNVTPAAEFNVWSDPEAASLVAQAGFRIRWVGLDVTELVRLERSRWERLGNATDPRSILAREVGRWAFEQRGLTSFALHD
PLAVVAAVREEFIDWAPGHVIVRTETDEVAGKTVYQSDPDGRARVAHRVNSDGAQAFLERALGLVGKGG
>Mature_309_residues
MKTMQPVIVDVDTGVDDALALAVALAQPELRLIAVTTVAGNVDVGQATRNTRLVLDWLGARSIPVAKGAASPLLRKHRDA
RAYHSPDGLGGAVLPVERGGPILDTSAPEVIVRAARRYAGKLCLVCLAPLTNLAIALRLEPDLPFLIDRVIVMGGAFAVA
GNVTPAAEFNVWSDPEAASLVAQAGFRIRWVGLDVTELVRLERSRWERLGNATDPRSILAREVGRWAFEQRGLTSFALHD
PLAVVAAVREEFIDWAPGHVIVRTETDEVAGKTVYQSDPDGRARVAHRVNSDGAQAFLERALGLVGKGG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=274, Percent_Identity=35.7664233576642, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1786213, Length=305, Percent_Identity=36.0655737704918, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1788486, Length=273, Percent_Identity=31.1355311355311, Blast_Score=124, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI32565474, Length=259, Percent_Identity=30.1158301158301, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17565698, Length=276, Percent_Identity=28.9855072463768, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=270, Percent_Identity=28.8888888888889, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24641839, Length=264, Percent_Identity=31.4393939393939, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24583915, Length=191, Percent_Identity=34.5549738219895, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641837, Length=183, Percent_Identity=32.2404371584699, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33088; Mature: 33088

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMQPVIVDVDTGVDDALALAVALAQPELRLIAVTTVAGNVDVGQATRNTRLVLDWLGA
CCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCEEEEEECCC
RSIPVAKGAASPLLRKHRDARAYHSPDGLGGAVLPVERGGPILDTSAPEVIVRAARRYAG
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
KLCLVCLAPLTNLAIALRLEPDLPFLIDRVIVMGGAFAVAGNVTPAAEFNVWSDPEAASL
HHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHH
VAQAGFRIRWVGLDVTELVRLERSRWERLGNATDPRSILAREVGRWAFEQRGLTSFALHD
HHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
PLAVVAAVREEFIDWAPGHVIVRTETDEVAGKTVYQSDPDGRARVAHRVNSDGAQAFLER
HHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCEEECCCCCCHHHHHHHCCCCHHHHHHHH
ALGLVGKGG
HHHHCCCCC
>Mature Secondary Structure
MKTMQPVIVDVDTGVDDALALAVALAQPELRLIAVTTVAGNVDVGQATRNTRLVLDWLGA
CCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCEEEEEECCC
RSIPVAKGAASPLLRKHRDARAYHSPDGLGGAVLPVERGGPILDTSAPEVIVRAARRYAG
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
KLCLVCLAPLTNLAIALRLEPDLPFLIDRVIVMGGAFAVAGNVTPAAEFNVWSDPEAASL
HHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHH
VAQAGFRIRWVGLDVTELVRLERSRWERLGNATDPRSILAREVGRWAFEQRGLTSFALHD
HHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
PLAVVAAVREEFIDWAPGHVIVRTETDEVAGKTVYQSDPDGRARVAHRVNSDGAQAFLER
HHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCEEECCCCCCHHHHHHHCCCCHHHHHHHH
ALGLVGKGG
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA