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The map label for this gene is metK [H]

Identifier: 221632915

GI number: 221632915

Start: 897477

End: 898715

Strand: Reverse

Name: metK [H]

Synonym: trd_0917

Alternate gene names: 221632915

Gene position: 898715-897477 (Counterclockwise)

Preceding gene: 221632916

Following gene: 221632914

Centisome position: 44.87

GC content: 62.15

Gene sequence:

>1239_bases
TTGGGAACGGTGACACTGAGCATCATGCGTGCCTCACGCACGTACTTTACCTCGGAGTCGGTGACCGAGGGACATCCGGA
CAAGCTGTGCGATCAGGTTTCCGATGCGGTATTGGATGCCGTCCTAGCGCAGGACCCGGACGGGCGAGTCGCTTGTGAAG
CGGCGACGACGACCGGGCTCATGATTCTCATCGGGGAAATCACGACAACGGCGCGCGTTGACTATGCTGAACTCGCTCGG
CAGGTCGTCGCCGATGCTGGCTATGTTTCCTCCGAGTACGGTATCGATGGACGGACGATGGGGGTTATCCTCTCGATCAA
GGAGCAGGCACCGGAGATCGCTTATGGAGTTTCCGAGTCGCTCGAAGTGCGCGAGGGTTCGGCAAGTGACGAGTTCGATC
GGATCGGCGCGGGCGACCAGGGAATGGTCGTCGGCTTTGCGTGCACCGAGACACCGGAGCTGATGCCGCTTCCCATCGCA
CTCGCGCATGGCCTGGTGCGTCGTTTAGCGACCGTTCGCAAGACGGGAATTCTCCCGTATCTCCGTCCGGACGGGAAGAG
CCAGGTGACGGTCGAGTATCGCTATGGGCGACCAGCTCGCGTCGCTACTGTGGTGCTTTCTGCCCAGCATGACCCAGATG
TCTCGCGCGAGCAACTCCGTCGCGATCTCATCGAGGCTGTCGTGAGTCAGGTGGTTCCAGGCGAGTTGCTCGATCGCGAG
ACGGAGATCCTGATCAATCCGAGCGGCTCCTTCGTCCTCGGTGGCCCTCGCGCGGACGCGGGGATGACCGGTCGCAAGAT
CATGGTGGACACCTACGGTGGGATGGCGCGTCATGGCGGCGGAGCCTTCTCCGGGAAAGATCCGACGAAGGTGGATCGCT
CGGCGGCATACGCTGCTCGCTACGTCGCTAAAAATGTCGTTGCCGCAGGACTGGCTGATCGCTTCGAGATTCAAATTTCG
TACGCCATCGGCCGAGCACGCCCGGTGGCGATCCATGTCGAGACGTTCGGGACGGGACGATTGGATGAGGAGCGGATCAC
GGAGCTCGTGACGAGGCATTTCGATCTCCGTCCGGCGGCGATCATCCATCATCTGGGCTTGCAGCGGCCGCTCTACCGAC
AGGTCGCGGCCTACGGACACTTCGGGCGACCCGATCTCGATTTGCCCTGGGAGCGGACGGACAAGGCAGAGTTGCTTCGG
TCGGAAGCGGGGCTGGTCGGAAGCCTACCGGAGCGATGA

Upstream 100 bases:

>100_bases
CCGGTTGACACCGGAACAAGAGGCCTATCTCGTCTCCTGGCAGCATGGGACATGACGGCGACGTGCGGTACACGAGGTGT
GACGTGACGAAGCGAAGGGG

Downstream 100 bases:

>100_bases
CGGAAGAGTGGGCGCTCCTATGCGGCGAGCCTTGATCACCGGTGCGACCGGGTTCGCTGGACGGTACCTCGCGGATCGAC
TGGCCGCAACGGGTCATTGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MGTVTLSIMRASRTYFTSESVTEGHPDKLCDQVSDAVLDAVLAQDPDGRVACEAATTTGLMILIGEITTTARVDYAELAR
QVVADAGYVSSEYGIDGRTMGVILSIKEQAPEIAYGVSESLEVREGSASDEFDRIGAGDQGMVVGFACTETPELMPLPIA
LAHGLVRRLATVRKTGILPYLRPDGKSQVTVEYRYGRPARVATVVLSAQHDPDVSREQLRRDLIEAVVSQVVPGELLDRE
TEILINPSGSFVLGGPRADAGMTGRKIMVDTYGGMARHGGGAFSGKDPTKVDRSAAYAARYVAKNVVAAGLADRFEIQIS
YAIGRARPVAIHVETFGTGRLDEERITELVTRHFDLRPAAIIHHLGLQRPLYRQVAAYGHFGRPDLDLPWERTDKAELLR
SEAGLVGSLPER

Sequences:

>Translated_412_residues
MGTVTLSIMRASRTYFTSESVTEGHPDKLCDQVSDAVLDAVLAQDPDGRVACEAATTTGLMILIGEITTTARVDYAELAR
QVVADAGYVSSEYGIDGRTMGVILSIKEQAPEIAYGVSESLEVREGSASDEFDRIGAGDQGMVVGFACTETPELMPLPIA
LAHGLVRRLATVRKTGILPYLRPDGKSQVTVEYRYGRPARVATVVLSAQHDPDVSREQLRRDLIEAVVSQVVPGELLDRE
TEILINPSGSFVLGGPRADAGMTGRKIMVDTYGGMARHGGGAFSGKDPTKVDRSAAYAARYVAKNVVAAGLADRFEIQIS
YAIGRARPVAIHVETFGTGRLDEERITELVTRHFDLRPAAIIHHLGLQRPLYRQVAAYGHFGRPDLDLPWERTDKAELLR
SEAGLVGSLPER
>Mature_411_residues
GTVTLSIMRASRTYFTSESVTEGHPDKLCDQVSDAVLDAVLAQDPDGRVACEAATTTGLMILIGEITTTARVDYAELARQ
VVADAGYVSSEYGIDGRTMGVILSIKEQAPEIAYGVSESLEVREGSASDEFDRIGAGDQGMVVGFACTETPELMPLPIAL
AHGLVRRLATVRKTGILPYLRPDGKSQVTVEYRYGRPARVATVVLSAQHDPDVSREQLRRDLIEAVVSQVVPGELLDRET
EILINPSGSFVLGGPRADAGMTGRKIMVDTYGGMARHGGGAFSGKDPTKVDRSAAYAARYVAKNVVAAGLADRFEIQISY
AIGRARPVAIHVETFGTGRLDEERITELVTRHFDLRPAAIIHHLGLQRPLYRQVAAYGHFGRPDLDLPWERTDKAELLRS
EAGLVGSLPER

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=52.8947368421053, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=51.8421052631579, Blast_Score=384, Evalue=1e-107,
Organism=Escherichia coli, GI1789311, Length=394, Percent_Identity=55.3299492385787, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25145633, Length=394, Percent_Identity=52.2842639593909, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17538498, Length=388, Percent_Identity=52.8350515463918, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17538494, Length=388, Percent_Identity=52.0618556701031, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=52.0725388601036, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32565905, Length=388, Percent_Identity=46.9072164948454, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=49.8542274052478, Blast_Score=324, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=50.4451038575668, Blast_Score=323, Evalue=9e-89,
Organism=Caenorhabditis elegans, GI32565903, Length=310, Percent_Identity=49.3548387096774, Blast_Score=291, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI32565909, Length=89, Percent_Identity=57.3033707865169, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=70.4545454545455, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=381, Percent_Identity=51.4435695538058, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6320710, Length=395, Percent_Identity=51.3924050632911, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45552159, Length=387, Percent_Identity=49.6124031007752, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580529, Length=387, Percent_Identity=49.6124031007752, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580537, Length=387, Percent_Identity=49.6124031007752, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580533, Length=387, Percent_Identity=49.6124031007752, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580535, Length=387, Percent_Identity=49.6124031007752, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580531, Length=387, Percent_Identity=49.6124031007752, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580527, Length=387, Percent_Identity=48.8372093023256, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580525, Length=387, Percent_Identity=48.8372093023256, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580541, Length=376, Percent_Identity=47.6063829787234, Blast_Score=343, Evalue=9e-95,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=48.8, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44574; Mature: 44443

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTVTLSIMRASRTYFTSESVTEGHPDKLCDQVSDAVLDAVLAQDPDGRVACEAATTTGL
CCEEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCE
MILIGEITTTARVDYAELARQVVADAGYVSSEYGIDGRTMGVILSIKEQAPEIAYGVSES
EEEEECCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHCCCHHHHCCCCC
LEVREGSASDEFDRIGAGDQGMVVGFACTETPELMPLPIALAHGLVRRLATVRKTGILPY
CEECCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
LRPDGKSQVTVEYRYGRPARVATVVLSAQHDPDVSREQLRRDLIEAVVSQVVPGELLDRE
ECCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCC
TEILINPSGSFVLGGPRADAGMTGRKIMVDTYGGMARHGGGAFSGKDPTKVDRSAAYAAR
CEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHH
YVAKNVVAAGLADRFEIQISYAIGRARPVAIHVETFGTGRLDEERITELVTRHFDLRPAA
HHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHH
IIHHLGLQRPLYRQVAAYGHFGRPDLDLPWERTDKAELLRSEAGLVGSLPER
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GTVTLSIMRASRTYFTSESVTEGHPDKLCDQVSDAVLDAVLAQDPDGRVACEAATTTGL
CEEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCE
MILIGEITTTARVDYAELARQVVADAGYVSSEYGIDGRTMGVILSIKEQAPEIAYGVSES
EEEEECCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHCCCHHHHCCCCC
LEVREGSASDEFDRIGAGDQGMVVGFACTETPELMPLPIALAHGLVRRLATVRKTGILPY
CEECCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
LRPDGKSQVTVEYRYGRPARVATVVLSAQHDPDVSREQLRRDLIEAVVSQVVPGELLDRE
ECCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCC
TEILINPSGSFVLGGPRADAGMTGRKIMVDTYGGMARHGGGAFSGKDPTKVDRSAAYAAR
CEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHH
YVAKNVVAAGLADRFEIQISYAIGRARPVAIHVETFGTGRLDEERITELVTRHFDLRPAA
HHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHH
IIHHLGLQRPLYRQVAAYGHFGRPDLDLPWERTDKAELLRSEAGLVGSLPER
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA