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The map label for this gene is nfi [H]

Identifier: 221632829

GI number: 221632829

Start: 801106

End: 801834

Strand: Reverse

Name: nfi [H]

Synonym: trd_0823

Alternate gene names: 221632829

Gene position: 801834-801106 (Counterclockwise)

Preceding gene: 221632830

Following gene: 221632828

Centisome position: 40.03

GC content: 61.32

Gene sequence:

>729_bases
ATGATTTCGCCACACTTCGTGTCAGGAAAGCCAGCGCTCTCTCCGCTCGAACTCGAGGAACTCGCTCGTCTGCAGAAAGA
ACTGGCAGAGAAGGTAGTGGAAACTCCACTTCCCCAAACGCCCCGCTTCATTGCGGGCGCAGATATGCACCTGCGCGGCG
AGCGAGCGGTCGCAGTCGCCGTCTGCTTCGAACTTCTGAGCGAACCAACCAGCGACGAAGCCACGGACTTCCTGCCGCTC
CGCGAGGTCGAGCGCGCGATCGCCGAACAAAAGGTAACCTTTCCCTACATTCCAGGGTTTCTCAGCTTCCGAGAGGCGCC
AGTGATCATAGAAGCGGTTCGGGCTCTCGAACAGACACCGGACCTTCTCGTCGTCGACGGGCAAGGACGAGCGCATCCTC
GCCGCTGCGGTCTGGCGAGTCATGTGGGCGTCTTGCTCGACCTGCCGACGATCGGCGCGGCCAAATCTCGCTTGTACGGC
TCGTATCGCGAGCCGGGACCGAACCGAGGAGCCTGGACACCATTGGTGGCTGAGGAGGAAATTATCGGTGCAGCAGTTCG
CACGCGGCCCAGTGCCCGACCACTTATCGTGTCGGTCGGGCACCGTATCACACTCCTGGAAGCGATCCGCTGGGTGCTCC
GACTCAGTCGCTATCGTATTCCGGAACCGACACGATGGGCACACCGATATGCGAAAGCGGCAGCTGCCTCGAAGGACACG
GGTTGGTGA

Upstream 100 bases:

>100_bases
AGCCCGAAGGGAAGCGAGCGATGTCAGCCCTCGACTGGTGGCGCGGTACACGACTGGCAAACGGGACCTGTTTCGAATAA
CGTCGACTGCGGACAAGTGG

Downstream 100 bases:

>100_bases
CCATGGAGCGAGTGCTCGCTGGAGGATCGGTTCGCCGCGAGTTTTCGTCCGTCTCGGGGACCAAGGAGTCCGCAACAGTG
CGAATGTTCGCGTCACGCGG

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MISPHFVSGKPALSPLELEELARLQKELAEKVVETPLPQTPRFIAGADMHLRGERAVAVAVCFELLSEPTSDEATDFLPL
REVERAIAEQKVTFPYIPGFLSFREAPVIIEAVRALEQTPDLLVVDGQGRAHPRRCGLASHVGVLLDLPTIGAAKSRLYG
SYREPGPNRGAWTPLVAEEEIIGAAVRTRPSARPLIVSVGHRITLLEAIRWVLRLSRYRIPEPTRWAHRYAKAAAASKDT
GW

Sequences:

>Translated_242_residues
MISPHFVSGKPALSPLELEELARLQKELAEKVVETPLPQTPRFIAGADMHLRGERAVAVAVCFELLSEPTSDEATDFLPL
REVERAIAEQKVTFPYIPGFLSFREAPVIIEAVRALEQTPDLLVVDGQGRAHPRRCGLASHVGVLLDLPTIGAAKSRLYG
SYREPGPNRGAWTPLVAEEEIIGAAVRTRPSARPLIVSVGHRITLLEAIRWVLRLSRYRIPEPTRWAHRYAKAAAASKDT
GW
>Mature_242_residues
MISPHFVSGKPALSPLELEELARLQKELAEKVVETPLPQTPRFIAGADMHLRGERAVAVAVCFELLSEPTSDEATDFLPL
REVERAIAEQKVTFPYIPGFLSFREAPVIIEAVRALEQTPDLLVVDGQGRAHPRRCGLASHVGVLLDLPTIGAAKSRLYG
SYREPGPNRGAWTPLVAEEEIIGAAVRTRPSARPLIVSVGHRITLLEAIRWVLRLSRYRIPEPTRWAHRYAKAAAASKDT
GW

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=153, Percent_Identity=40.5228758169935, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI257467548, Length=119, Percent_Identity=40.3361344537815, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI257467550, Length=119, Percent_Identity=40.3361344537815, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI87082357, Length=221, Percent_Identity=42.5339366515837, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 26730; Mature: 26730

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPHFVSGKPALSPLELEELARLQKELAEKVVETPLPQTPRFIAGADMHLRGERAVAVA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEECCCHHHHHH
VCFELLSEPTSDEATDFLPLREVERAIAEQKVTFPYIPGFLSFREAPVIIEAVRALEQTP
HHHHHHCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCC
DLLVVDGQGRAHPRRCGLASHVGVLLDLPTIGAAKSRLYGSYREPGPNRGAWTPLVAEEE
CEEEECCCCCCCHHHCCHHHHHHHEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHH
IIGAAVRTRPSARPLIVSVGHRITLLEAIRWVLRLSRYRIPEPTRWAHRYAKAAAASKDT
HHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
GW
CC
>Mature Secondary Structure
MISPHFVSGKPALSPLELEELARLQKELAEKVVETPLPQTPRFIAGADMHLRGERAVAVA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEECCCHHHHHH
VCFELLSEPTSDEATDFLPLREVERAIAEQKVTFPYIPGFLSFREAPVIIEAVRALEQTP
HHHHHHCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCC
DLLVVDGQGRAHPRRCGLASHVGVLLDLPTIGAAKSRLYGSYREPGPNRGAWTPLVAEEE
CEEEECCCCCCCHHHCCHHHHHHHEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCHHH
IIGAAVRTRPSARPLIVSVGHRITLLEAIRWVLRLSRYRIPEPTRWAHRYAKAAAASKDT
HHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
GW
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA