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The map label for this gene is fmt [H]

Identifier: 221632830

GI number: 221632830

Start: 801855

End: 802796

Strand: Reverse

Name: fmt [H]

Synonym: trd_0824

Alternate gene names: 221632830

Gene position: 802796-801855 (Counterclockwise)

Preceding gene: 221632838

Following gene: 221632829

Centisome position: 40.08

GC content: 61.89

Gene sequence:

>942_bases
GTGGAGGGCAAACTTCGCCTGGTCTTCTTCGGAACACCGGAATTTGCCGTTCCTTCGCTCCGGACACTCGCGGCACAGCC
AGACTTTGTCGTCGTGCTGGTCGTCACCCAGCCGGATCGACCGGCGGGACGGGGTCGGGGACTCCAACCTCCGCCAGTGA
AGGTGCTCGCGCAAGAGCTGGGTCTTCCATGTTGGCAGCCAGAGACTCTTCGTACGGCCGAAGCAGAGGCGAGACTCGCC
GCCGTGGCACCGATGCTCGCCGTTGTGGTCGCCTACGGCAAAATCATCCCAGCATCGATGCTTTCGATGCCACGGCATGG
CTTCCTCAACGTGCATCCCTCACTGCTTCCGCGCTATCGCGGAGCCAGTCCGATCCAGGCGGCCTTGCTCAATGGTGATG
CGATCACTGGTATCTCGTTCGCCGTCATGACCCCCGAGTTGGATGCCGGTCCGATCCTTCGCCAATTCGCGATTCCTATC
GTGCCTGACGATACAGGCGTCACGCTGGGTGCTCGGCTAGCCGAGGTCGCGGCAGAACTCCTCCCTGACACGATCCGGGA
TTGGATCGCAGGCCGAATCGTGCCGATCCCTCAGGACGAAGGAGAAGCAACGTATACGCGTCCCCTCACCAAAGCCGATG
GCCGAGTGGACTGGTCGGAGCCAGCAGAGCGCATCGAGCGGAAGATCCGGGCATTGCAACCCTGGCCATGTGCGTGGACG
ACAATTGGCGGTCGTCGCCTGATTCTCCTAAGGGCGAGGCTGATCGAAACGGAGATGGACTTGGCTCCCGGTTGTCTTTC
GGTCATGCCAACTGTGGTGCTCGTCGGCACGGGAACGCGGCCACTCGCGCTGGAAACAGTTCAGCCCGAAGGGAAGCGAG
CGATGTCAGCCCTCGACTGGTGGCGCGGTACACGACTGGCAAACGGGACCTGTTTCGAATAA

Upstream 100 bases:

>100_bases
CTCCGTCCTCATAACCCCGCAAATGATGCCCGACTCCAGAGAGCCGTGGTAGGGTAGAACAGGGTGGAACGGTCGCGCAC
GATCTGGGGGGTCTCAGCCG

Downstream 100 bases:

>100_bases
CGTCGACTGCGGACAAGTGGATGATTTCGCCACACTTCGTGTCAGGAAAGCCAGCGCTCTCTCCGCTCGAACTCGAGGAA
CTCGCTCGTCTGCAGAAAGA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MEGKLRLVFFGTPEFAVPSLRTLAAQPDFVVVLVVTQPDRPAGRGRGLQPPPVKVLAQELGLPCWQPETLRTAEAEARLA
AVAPMLAVVVAYGKIIPASMLSMPRHGFLNVHPSLLPRYRGASPIQAALLNGDAITGISFAVMTPELDAGPILRQFAIPI
VPDDTGVTLGARLAEVAAELLPDTIRDWIAGRIVPIPQDEGEATYTRPLTKADGRVDWSEPAERIERKIRALQPWPCAWT
TIGGRRLILLRARLIETEMDLAPGCLSVMPTVVLVGTGTRPLALETVQPEGKRAMSALDWWRGTRLANGTCFE

Sequences:

>Translated_313_residues
MEGKLRLVFFGTPEFAVPSLRTLAAQPDFVVVLVVTQPDRPAGRGRGLQPPPVKVLAQELGLPCWQPETLRTAEAEARLA
AVAPMLAVVVAYGKIIPASMLSMPRHGFLNVHPSLLPRYRGASPIQAALLNGDAITGISFAVMTPELDAGPILRQFAIPI
VPDDTGVTLGARLAEVAAELLPDTIRDWIAGRIVPIPQDEGEATYTRPLTKADGRVDWSEPAERIERKIRALQPWPCAWT
TIGGRRLILLRARLIETEMDLAPGCLSVMPTVVLVGTGTRPLALETVQPEGKRAMSALDWWRGTRLANGTCFE
>Mature_313_residues
MEGKLRLVFFGTPEFAVPSLRTLAAQPDFVVVLVVTQPDRPAGRGRGLQPPPVKVLAQELGLPCWQPETLRTAEAEARLA
AVAPMLAVVVAYGKIIPASMLSMPRHGFLNVHPSLLPRYRGASPIQAALLNGDAITGISFAVMTPELDAGPILRQFAIPI
VPDDTGVTLGARLAEVAAELLPDTIRDWIAGRIVPIPQDEGEATYTRPLTKADGRVDWSEPAERIERKIRALQPWPCAWT
TIGGRRLILLRARLIETEMDLAPGCLSVMPTVVLVGTGTRPLALETVQPEGKRAMSALDWWRGTRLANGTCFE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI21614513, Length=295, Percent_Identity=29.8305084745763, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI238814322, Length=323, Percent_Identity=27.8637770897833, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI164663775, Length=239, Percent_Identity=31.7991631799163, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1789683, Length=306, Percent_Identity=45.0980392156863, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788589, Length=308, Percent_Identity=28.8961038961039, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788846, Length=111, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI133930964, Length=266, Percent_Identity=27.4436090225564, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319458, Length=282, Percent_Identity=29.7872340425532, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45550868, Length=309, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI28571984, Length=225, Percent_Identity=36.4444444444444, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24585660, Length=255, Percent_Identity=27.843137254902, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 33948; Mature: 33948

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGKLRLVFFGTPEFAVPSLRTLAAQPDFVVVLVVTQPDRPAGRGRGLQPPPVKVLAQEL
CCCCEEEEEECCCCCCCCCHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
GLPCWQPETLRTAEAEARLAAVAPMLAVVVAYGKIIPASMLSMPRHGFLNVHPSLLPRYR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHCCC
GASPIQAALLNGDAITGISFAVMTPELDAGPILRQFAIPIVPDDTGVTLGARLAEVAAEL
CCCCHHHEEECCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LPDTIRDWIAGRIVPIPQDEGEATYTRPLTKADGRVDWSEPAERIERKIRALQPWPCAWT
HHHHHHHHHCCCEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
TIGGRRLILLRARLIETEMDLAPGCLSVMPTVVLVGTGTRPLALETVQPEGKRAMSALDW
ECCCCEEEEEHHHHHHHHHCCCCHHHHHCCEEEEEECCCCCEEEEECCCCHHHHHHHHHH
WRGTRLANGTCFE
HCCCCCCCCCCCC
>Mature Secondary Structure
MEGKLRLVFFGTPEFAVPSLRTLAAQPDFVVVLVVTQPDRPAGRGRGLQPPPVKVLAQEL
CCCCEEEEEECCCCCCCCCHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
GLPCWQPETLRTAEAEARLAAVAPMLAVVVAYGKIIPASMLSMPRHGFLNVHPSLLPRYR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHCCC
GASPIQAALLNGDAITGISFAVMTPELDAGPILRQFAIPIVPDDTGVTLGARLAEVAAEL
CCCCHHHEEECCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LPDTIRDWIAGRIVPIPQDEGEATYTRPLTKADGRVDWSEPAERIERKIRALQPWPCAWT
HHHHHHHHHCCCEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
TIGGRRLILLRARLIETEMDLAPGCLSVMPTVVLVGTGTRPLALETVQPEGKRAMSALDW
ECCCCEEEEEHHHHHHHHHCCCCHHHHHCCEEEEEECCCCCEEEEECCCCHHHHHHHHHH
WRGTRLANGTCFE
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA