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The map label for this gene is folD

Identifier: 221632743

GI number: 221632743

Start: 723357

End: 724223

Strand: Reverse

Name: folD

Synonym: trd_0733

Alternate gene names: 221632743

Gene position: 724223-723357 (Counterclockwise)

Preceding gene: 221632744

Following gene: 221632741

Centisome position: 36.16

GC content: 62.75

Gene sequence:

>867_bases
ATGGCTGCCCACATACTATCGGGGAGACCAGTCGTCGAGTCGCTGCACGCCCGTATCCAGCAAGTGCTTGCACGGCGAGC
CCCACATCAGGGACCACCCACCCTCACCGTGCTGCTCCCCAACGATCCTTCCGCCCAAGCCTACGCTCGCGCGATCCGCA
AGCAATTCACCACACTGAGCTTGAATTATCGGACCGAAGAGATTGACGATACACTGGATGAATCTCGATTCGCGATTCTC
CTCAAGCGACTCGCCGAGGACGAATCCGTCACCGGCATCCTGGCGTTGCAGCCGCTCCCCAAAGGCGTCTCGCGCCGATC
GCTCGCACGACTGATGCCGCCCACCAAGGACGTCGACGGCGTCTCGTTCGAACAGCAAGGGCGTTTAGCGATCGGCAGTC
CTTGGATCGCGCCGAGCACCCCTCTCGGAGGACTTATCCTCCTCCAGCACTACGGCATCGAAGTGGCGGGTCGGCATGCT
GTCGTCATCGGTCGCAGCCCCGTCGTCGGTCGCCCGCTTGCCCTCTTGCTTCTGGCCCGCGACGCCACTGTGACCATTTG
CCATCGCCGTACGCCTGATCTCGCCAAGCTCACGCGACAGGCCGATCTCCTGTTCGTCGCTGCCGGCCAACCCCATCTCG
TCAAGCCGGACATGGTCGCTCCAGGAGCCGTGGTTATCGATTTCGGGGTCTCGGTGCGCGACGGCCGTCTCATCGGTGAT
GTCGATCCAGCCGTCGCCGAAGTGGCCAGCGCTCTTACCCCCGTTCCTGGCGGCACGGGTCCAGTCACCACTGCCGTACT
GGCCCTCAACCTTCTTCGATTAGCGGGACTTCTCGAGGAAAACGATCTCTTCCCAGGCGCTCCTTGA

Upstream 100 bases:

>100_bases
CGGAGCACGCTCAGGCCTGAGGATCATGCGTGCACGCTACTGCACAAATGACCTGGCGTCCCATCGAAAGACTTCTGTCA
CGCGGAAAGGATGGAGAGCG

Downstream 100 bases:

>100_bases
GCGGAGACACGCAGCGAGGGCGAACCTGCTCGTCCCCGCCAAAGCTCCATGGTCGACCGCGACGAACGCACAGTCATCCT
CGAAGCGAGACTCCGCCACG

Product: 5,10-methylene-tetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MAAHILSGRPVVESLHARIQQVLARRAPHQGPPTLTVLLPNDPSAQAYARAIRKQFTTLSLNYRTEEIDDTLDESRFAIL
LKRLAEDESVTGILALQPLPKGVSRRSLARLMPPTKDVDGVSFEQQGRLAIGSPWIAPSTPLGGLILLQHYGIEVAGRHA
VVIGRSPVVGRPLALLLLARDATVTICHRRTPDLAKLTRQADLLFVAAGQPHLVKPDMVAPGAVVIDFGVSVRDGRLIGD
VDPAVAEVASALTPVPGGTGPVTTAVLALNLLRLAGLLEENDLFPGAP

Sequences:

>Translated_288_residues
MAAHILSGRPVVESLHARIQQVLARRAPHQGPPTLTVLLPNDPSAQAYARAIRKQFTTLSLNYRTEEIDDTLDESRFAIL
LKRLAEDESVTGILALQPLPKGVSRRSLARLMPPTKDVDGVSFEQQGRLAIGSPWIAPSTPLGGLILLQHYGIEVAGRHA
VVIGRSPVVGRPLALLLLARDATVTICHRRTPDLAKLTRQADLLFVAAGQPHLVKPDMVAPGAVVIDFGVSVRDGRLIGD
VDPAVAEVASALTPVPGGTGPVTTAVLALNLLRLAGLLEENDLFPGAP
>Mature_287_residues
AAHILSGRPVVESLHARIQQVLARRAPHQGPPTLTVLLPNDPSAQAYARAIRKQFTTLSLNYRTEEIDDTLDESRFAILL
KRLAEDESVTGILALQPLPKGVSRRSLARLMPPTKDVDGVSFEQQGRLAIGSPWIAPSTPLGGLILLQHYGIEVAGRHAV
VIGRSPVVGRPLALLLLARDATVTICHRRTPDLAKLTRQADLLFVAAGQPHLVKPDMVAPGAVVIDFGVSVRDGRLIGDV
DPAVAEVASALTPVPGGTGPVTTAVLALNLLRLAGLLEENDLFPGAP

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=281, Percent_Identity=36.6548042704626, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI94721354, Length=287, Percent_Identity=34.8432055749129, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=36.6101694915254, Blast_Score=138, Evalue=6e-33,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=37.5451263537906, Blast_Score=171, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=35.958904109589, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=35.0877192982456, Blast_Score=158, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6321643, Length=293, Percent_Identity=31.740614334471, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=37.3287671232877, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=37.3287671232877, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=36.4583333333333, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=36.4583333333333, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI62472483, Length=293, Percent_Identity=36.1774744027304, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI45551871, Length=293, Percent_Identity=36.1774744027304, Blast_Score=157, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_THERP (B9KZ23)

Other databases:

- EMBL:   CP001275
- RefSeq:   YP_002521965.1
- GeneID:   7356214
- GenomeReviews:   CP001275_GR
- KEGG:   tro:trd_0733
- OMA:   VESLHAR
- ProtClustDB:   CLSK2807658
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30631; Mature: 30500

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAHILSGRPVVESLHARIQQVLARRAPHQGPPTLTVLLPNDPSAQAYARAIRKQFTTLS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEE
LNYRTEEIDDTLDESRFAILLKRLAEDESVTGILALQPLPKGVSRRSLARLMPPTKDVDG
EECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCC
VSFEQQGRLAIGSPWIAPSTPLGGLILLQHYGIEVAGRHAVVIGRSPVVGRPLALLLLAR
CCCCCCCCEEECCCCCCCCCCCCCEEEEEECCEEECCCEEEEEECCCCCCCCCEEEEEEC
DATVTICHRRTPDLAKLTRQADLLFVAAGQPHLVKPDMVAPGAVVIDFGVSVRDGRLIGD
CCEEEEEECCCCHHHHHHCCCCEEEEECCCCCEECCCCCCCCEEEEEECCEEECCEEECC
VDPAVAEVASALTPVPGGTGPVTTAVLALNLLRLAGLLEENDLFPGAP
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AAHILSGRPVVESLHARIQQVLARRAPHQGPPTLTVLLPNDPSAQAYARAIRKQFTTLS
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEE
LNYRTEEIDDTLDESRFAILLKRLAEDESVTGILALQPLPKGVSRRSLARLMPPTKDVDG
EECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCC
VSFEQQGRLAIGSPWIAPSTPLGGLILLQHYGIEVAGRHAVVIGRSPVVGRPLALLLLAR
CCCCCCCCEEECCCCCCCCCCCCCEEEEEECCEEECCCEEEEEECCCCCCCCCEEEEEEC
DATVTICHRRTPDLAKLTRQADLLFVAAGQPHLVKPDMVAPGAVVIDFGVSVRDGRLIGD
CCEEEEEECCCCHHHHHHCCCCEEEEECCCCCEECCCCCCCCEEEEEECCEEECCEEECC
VDPAVAEVASALTPVPGGTGPVTTAVLALNLLRLAGLLEENDLFPGAP
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA