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The map label for this gene is potD [H]

Identifier: 221632545

GI number: 221632545

Start: 516833

End: 518038

Strand: Reverse

Name: potD [H]

Synonym: trd_0520

Alternate gene names: 221632545

Gene position: 518038-516833 (Counterclockwise)

Preceding gene: 221632546

Following gene: 221632542

Centisome position: 25.86

GC content: 58.04

Gene sequence:

>1206_bases
ATGAATCGACCGGAACTCATCCAGCGATGGATCCGCGATTGGCAGCAGGGTCGGCTCTCGCGCCGTGAACTCTTGCGGCG
AGTGACACTGCTCGGCATGAGTTCGTTCGCGCTCGCCAACTTGCTCACTGCCTGCGCTCGATCGCAGCCGACGCCGACAC
CCGCGTCCACTGAGGCATCACCGCGCGTTGGGGCTGCAATCCCTGACTATATCGATCGCAGCAAGCTGGACAAGGAACTC
AACTTCTACAACTGGTCGGAGTACATCGATCCGGACATCCTGACCGAGTTCCAAGAGACCTATGGCGTCAAGGTCACGAT
GGACACCTACGCTTCCAACGAGGATCTACTCGCCAAGCTCCAAGGTGGCGCGACGGGCTACGACGTTATCGTTCCCTCCG
ACTACATGGTCAGCATCATGATCAACCTCGGCCTCCTCGAGCCACTCGACTACTCTGTCATCAAGACGATCGCGAACATC
GACCCCGAGAATCTGAAACCGTACTATGACCCCGACAACACCTATAGCGTGCCGTACATGTGGGGTACCTCCGGTTACAG
CTACGATACCAGTGTGCTCGGCGAAGACCTCGCCAGCTGGAAAGAAGTGTTCGAACCACGACCCGAGGTGCGCGGCAAGG
TGGTCATGTTGGATGACCAGCGCGAGGTGATCGGGGCCGCCCTCATGTATTTGGGCTACGAGATCAACGAGACCAGCGAT
GACGCACTCGCTAAAGCGAAGCAACTACTCCTCACGCAGAAGCCGTACGTGCTCGCCTATTCCAGCCAGAACAACGATGA
CTTGCTCGTCGCGGGCGAAGCACTGATCGCCCACATCTGGACCGGCGATGCGCTCATGAGCGAGCAAGAGAAGCCGACCC
TCCGCTATGTCATCCCTACCGAAGGTTGCACGGTCTGGCAGGACAACCTTTGTGTTCCCAAGACGGCCCCGCACAAGTAC
ACGGCGATGGTCTTCATCGACTTTCTCAACCGGCCCGACATCGCTGGGCGCAACGCCAACTACATCATGTACGGGAGCCC
GAACAAAGCAGCCCGAGAGCAAGGCTATATCGACCAAGAAGTGCTCAACAATCCGGGTGTTTATCCGCCACCGGAGGTTT
GGAAGCGACTTCAATGGATCAAGGACGTCGGTGAAGAGGGCGCTCTCAAGCTGGATCGTCTCTGGACAGAGATCAAGACG
GGCTGA

Upstream 100 bases:

>100_bases
CAATGCGCTGTCCACCGTGATGCTGCTTGCCTCCATCGTGCTCGTTTTAGGATCGCTCCTGTTCCAGCGTCGTCGGTCGT
TCTGACATGAGGAGGAGACG

Downstream 100 bases:

>100_bases
GCTCCGCGTGCCTTCTGGGTAGGAATGCGATTCACCCTCGCTGCTTCGGCATCCCTTTGTCTGTGCGGTCGCGACACGAG
GGGCATCCTTCAAGGGAATG

Product: spermidine/putrescine-binding periplasmic protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNRPELIQRWIRDWQQGRLSRRELLRRVTLLGMSSFALANLLTACARSQPTPTPASTEASPRVGAAIPDYIDRSKLDKEL
NFYNWSEYIDPDILTEFQETYGVKVTMDTYASNEDLLAKLQGGATGYDVIVPSDYMVSIMINLGLLEPLDYSVIKTIANI
DPENLKPYYDPDNTYSVPYMWGTSGYSYDTSVLGEDLASWKEVFEPRPEVRGKVVMLDDQREVIGAALMYLGYEINETSD
DALAKAKQLLLTQKPYVLAYSSQNNDDLLVAGEALIAHIWTGDALMSEQEKPTLRYVIPTEGCTVWQDNLCVPKTAPHKY
TAMVFIDFLNRPDIAGRNANYIMYGSPNKAAREQGYIDQEVLNNPGVYPPPEVWKRLQWIKDVGEEGALKLDRLWTEIKT
G

Sequences:

>Translated_401_residues
MNRPELIQRWIRDWQQGRLSRRELLRRVTLLGMSSFALANLLTACARSQPTPTPASTEASPRVGAAIPDYIDRSKLDKEL
NFYNWSEYIDPDILTEFQETYGVKVTMDTYASNEDLLAKLQGGATGYDVIVPSDYMVSIMINLGLLEPLDYSVIKTIANI
DPENLKPYYDPDNTYSVPYMWGTSGYSYDTSVLGEDLASWKEVFEPRPEVRGKVVMLDDQREVIGAALMYLGYEINETSD
DALAKAKQLLLTQKPYVLAYSSQNNDDLLVAGEALIAHIWTGDALMSEQEKPTLRYVIPTEGCTVWQDNLCVPKTAPHKY
TAMVFIDFLNRPDIAGRNANYIMYGSPNKAAREQGYIDQEVLNNPGVYPPPEVWKRLQWIKDVGEEGALKLDRLWTEIKT
G
>Mature_401_residues
MNRPELIQRWIRDWQQGRLSRRELLRRVTLLGMSSFALANLLTACARSQPTPTPASTEASPRVGAAIPDYIDRSKLDKEL
NFYNWSEYIDPDILTEFQETYGVKVTMDTYASNEDLLAKLQGGATGYDVIVPSDYMVSIMINLGLLEPLDYSVIKTIANI
DPENLKPYYDPDNTYSVPYMWGTSGYSYDTSVLGEDLASWKEVFEPRPEVRGKVVMLDDQREVIGAALMYLGYEINETSD
DALAKAKQLLLTQKPYVLAYSSQNNDDLLVAGEALIAHIWTGDALMSEQEKPTLRYVIPTEGCTVWQDNLCVPKTAPHKY
TAMVFIDFLNRPDIAGRNANYIMYGSPNKAAREQGYIDQEVLNNPGVYPPPEVWKRLQWIKDVGEEGALKLDRLWTEIKT
G

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=324, Percent_Identity=35.8024691358025, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787078, Length=342, Percent_Identity=34.5029239766082, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787711, Length=296, Percent_Identity=23.6486486486486, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45382; Mature: 45382

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPELIQRWIRDWQQGRLSRRELLRRVTLLGMSSFALANLLTACARSQPTPTPASTEAS
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
PRVGAAIPDYIDRSKLDKELNFYNWSEYIDPDILTEFQETYGVKVTMDTYASNEDLLAKL
CCCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHC
QGGATGYDVIVPSDYMVSIMINLGLLEPLDYSVIKTIANIDPENLKPYYDPDNTYSVPYM
CCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHCCCCCCCCCCEECCEE
WGTSGYSYDTSVLGEDLASWKEVFEPRPEVRGKVVMLDDQREVIGAALMYLGYEINETSD
ECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCH
DALAKAKQLLLTQKPYVLAYSSQNNDDLLVAGEALIAHIWTGDALMSEQEKPTLRYVIPT
HHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCEEEEEEECCHHHHCCCCCCEEEEEEEC
EGCTVWQDNLCVPKTAPHKYTAMVFIDFLNRPDIAGRNANYIMYGSPNKAAREQGYIDQE
CCCEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHCCCCCHH
VLNNPGVYPPPEVWKRLQWIKDVGEEGALKLDRLWTEIKTG
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNRPELIQRWIRDWQQGRLSRRELLRRVTLLGMSSFALANLLTACARSQPTPTPASTEAS
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
PRVGAAIPDYIDRSKLDKELNFYNWSEYIDPDILTEFQETYGVKVTMDTYASNEDLLAKL
CCCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHC
QGGATGYDVIVPSDYMVSIMINLGLLEPLDYSVIKTIANIDPENLKPYYDPDNTYSVPYM
CCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHCCCCCCCCCCEECCEE
WGTSGYSYDTSVLGEDLASWKEVFEPRPEVRGKVVMLDDQREVIGAALMYLGYEINETSD
ECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCH
DALAKAKQLLLTQKPYVLAYSSQNNDDLLVAGEALIAHIWTGDALMSEQEKPTLRYVIPT
HHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCEEEEEEECCHHHHCCCCCCEEEEEEEC
EGCTVWQDNLCVPKTAPHKYTAMVFIDFLNRPDIAGRNANYIMYGSPNKAAREQGYIDQE
CCCEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHCCCCCHH
VLNNPGVYPPPEVWKRLQWIKDVGEEGALKLDRLWTEIKTG
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]