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The map label for this gene is potC [H]

Identifier: 221632546

GI number: 221632546

Start: 518054

End: 518914

Strand: Reverse

Name: potC [H]

Synonym: trd_0521

Alternate gene names: 221632546

Gene position: 518914-518054 (Counterclockwise)

Preceding gene: 221632547

Following gene: 221632545

Centisome position: 25.91

GC content: 58.54

Gene sequence:

>861_bases
ATGAGTTCGATGGCAACGGCACGACCAGCGACGACGATGCGGAGCAAACGCCGTTTTTCTTTCGGGTGGGCAGTGCTCAA
TCTCAATGTATTACTAACTTTCATTTTCTTGTACGCCCCCATCATCGTGATGTCGATCTTCAGCTTCAATGCCTCCCGCC
AGATGCAAGTCTGGATGGGTTTCACGCTCGATTGGTACGCGCGCATGATCGCTGACGAGCGGGTCCGAGTCGCCGCGCAG
AACTCGTTGGTGATCGCTCTGGTCTCGACGATCGTCTCGACGGTGATCGGGACACTCAGCGCGCTTGCCTTGGAACGCAC
GCGTTTTCGCGTCAAGCGCATGTACGAAGCCGCGATGTATCTGCCGATCATCGTGCCCGAAATCGTGATGGCTATTGCCC
TGCTGGTCTTCTTCAATCTGGCCTTTACCTGGCTGGACCAGTTCTTCGGTCTCAAGCTCAAGTTTGGTCTCGGGACGATC
ACGATCGCCCACATCGCCTTCTCTTTCCCCTTCGTCGCTGTCGTCGTGCGCGCCCGTCTGGCAGACTTTGATCGCACACT
GGAACAGGCCGCTCAGGATCTCGGTGCGAACGAGTGGCAGACGTTCCGACACATCACGCTCCCGCTCCTCATGCCCGGCA
TCATCGCCGGAGCGTTGTTGGCGTTCACCCTCTCGATCGACGACTTCGTCATCACGTTCTTCACAGCCGGCGTGGGCTCG
ACGACGCTGCCGCTCGAGATCTACGGCCGAGTGCGGCGCGGTATCACGCCGGATGTCAATGCGCTGTCCACCGTGATGCT
GCTTGCCTCCATCGTGCTCGTTTTAGGATCGCTCCTGTTCCAGCGTCGTCGGTCGTTCTGA

Upstream 100 bases:

>100_bases
TGCGACACTGGCCCTTCGGTTCAGCGATTTCGTTCGTCTTGATGGCGATCGTTCTCGCAGCGACCATGCTCTACTTCCGA
ATCGGGGAGACGCGGCGGAT

Downstream 100 bases:

>100_bases
CATGAGGAGGAGACGATGAATCGACCGGAACTCATCCAGCGATGGATCCGCGATTGGCAGCAGGGTCGGCTCTCGCGCCG
TGAACTCTTGCGGCGAGTGA

Product: spermidine/putrescine ABC transporter permease

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSSMATARPATTMRSKRRFSFGWAVLNLNVLLTFIFLYAPIIVMSIFSFNASRQMQVWMGFTLDWYARMIADERVRVAAQ
NSLVIALVSTIVSTVIGTLSALALERTRFRVKRMYEAAMYLPIIVPEIVMAIALLVFFNLAFTWLDQFFGLKLKFGLGTI
TIAHIAFSFPFVAVVVRARLADFDRTLEQAAQDLGANEWQTFRHITLPLLMPGIIAGALLAFTLSIDDFVITFFTAGVGS
TTLPLEIYGRVRRGITPDVNALSTVMLLASIVLVLGSLLFQRRRSF

Sequences:

>Translated_286_residues
MSSMATARPATTMRSKRRFSFGWAVLNLNVLLTFIFLYAPIIVMSIFSFNASRQMQVWMGFTLDWYARMIADERVRVAAQ
NSLVIALVSTIVSTVIGTLSALALERTRFRVKRMYEAAMYLPIIVPEIVMAIALLVFFNLAFTWLDQFFGLKLKFGLGTI
TIAHIAFSFPFVAVVVRARLADFDRTLEQAAQDLGANEWQTFRHITLPLLMPGIIAGALLAFTLSIDDFVITFFTAGVGS
TTLPLEIYGRVRRGITPDVNALSTVMLLASIVLVLGSLLFQRRRSF
>Mature_285_residues
SSMATARPATTMRSKRRFSFGWAVLNLNVLLTFIFLYAPIIVMSIFSFNASRQMQVWMGFTLDWYARMIADERVRVAAQN
SLVIALVSTIVSTVIGTLSALALERTRFRVKRMYEAAMYLPIIVPEIVMAIALLVFFNLAFTWLDQFFGLKLKFGLGTIT
IAHIAFSFPFVAVVVRARLADFDRTLEQAAQDLGANEWQTFRHITLPLLMPGIIAGALLAFTLSIDDFVITFFTAGVGST
TLPLEIYGRVRRGITPDVNALSTVMLLASIVLVLGSLLFQRRRSF

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=238, Percent_Identity=42.436974789916, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1787081, Length=241, Percent_Identity=40.2489626556017, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1787714, Length=252, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788764, Length=207, Percent_Identity=31.4009661835749, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786980, Length=141, Percent_Identity=36.8794326241135, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787369, Length=187, Percent_Identity=27.807486631016, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787713, Length=169, Percent_Identity=30.7692307692308, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32013; Mature: 31881

Theoretical pI: Translated: 11.29; Mature: 11.29

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSMATARPATTMRSKRRFSFGWAVLNLNVLLTFIFLYAPIIVMSIFSFNASRQMQVWMG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
FTLDWYARMIADERVRVAAQNSLVIALVSTIVSTVIGTLSALALERTRFRVKRMYEAAMY
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPIIVPEIVMAIALLVFFNLAFTWLDQFFGLKLKFGLGTITIAHIAFSFPFVAVVVRARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
ADFDRTLEQAAQDLGANEWQTFRHITLPLLMPGIIAGALLAFTLSIDDFVITFFTAGVGS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TTLPLEIYGRVRRGITPDVNALSTVMLLASIVLVLGSLLFQRRRSF
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSMATARPATTMRSKRRFSFGWAVLNLNVLLTFIFLYAPIIVMSIFSFNASRQMQVWMG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
FTLDWYARMIADERVRVAAQNSLVIALVSTIVSTVIGTLSALALERTRFRVKRMYEAAMY
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPIIVPEIVMAIALLVFFNLAFTWLDQFFGLKLKFGLGTITIAHIAFSFPFVAVVVRARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
ADFDRTLEQAAQDLGANEWQTFRHITLPLLMPGIIAGALLAFTLSIDDFVITFFTAGVGS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TTLPLEIYGRVRRGITPDVNALSTVMLLASIVLVLGSLLFQRRRSF
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]