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The map label for this gene is rumA [C]

Identifier: 221632250

GI number: 221632250

Start: 226264

End: 227484

Strand: Reverse

Name: rumA [C]

Synonym: trd_0216

Alternate gene names: 221632250

Gene position: 227484-226264 (Counterclockwise)

Preceding gene: 221632253

Following gene: 221632249

Centisome position: 11.36

GC content: 63.14

Gene sequence:

>1221_bases
ATGCCGATGGGGGAGACATTACCGACACCACGCACCCTACGGACCGTTCCGCTCCATTTGGAGGACATGGCTTACCAGGG
CGCGGCGATCGGGCGCGAAGACGGTCGAATCGTCTTCGCCGAGTACGGGATTCCTGGCGAAGACGTACTCGTCGCGATCG
AGAAGGACCGCAAGGACCACTCGCTCGGGCGCGTCATCGAGGTGCGGACCGCTTCACCGGAGCGGGTCGATCCTCCCTGC
CCATACTTCGGTATCTGTGGCGGATGCCAGTGGCAGCACATCCGTTACGAATACCAACTCGTGCTCAAGCAGGCCGTCGT
GCGCCAGCAGTTGCGCCGCATCGGCAAGTTCGACGATCCGCCTGTCTTGCCCACTGTCCCCTCGCCGCAGCCGTACGGTT
ATCGAAACCACGCTCGCTTCTCGGTCGATGGCAAGGGGAACCTCGGGTACATCTCGCGCCCCGGCCATGGCTACCGCTTC
CTCCGCATCGATCGCTGCCTCATCATGCATCCAGCGATCAACGAGGTCCTGAGGCGCCTCCAGGAACGAGCACACGTCAA
GCACCAGCTCATGGTCCGATACGGAGTCAATACTGGCGAGCTTTTGGTGCATCCGGACGTCAGCGCGATCGATCCGACGA
TTCCCTCCGGCCAACCCGCTTACCACGAAAGTCTGCTCGGGCATCGCTTCCGGATTTCGGCCTCGTCCTTTTTCCAGACC
AACACTGCGGTCGCCGAGCGCCTCATCCAGCTCGTCTTGGAACGGATCGCTCCGACGGGGAACGAGGTCGTCGTCGATGC
CTATGCGGGTGTCGGCACCTTCGCCGCTTTTCTCGCCCCACGCGTCGCGCGAGTGATCGGGATCGAGGAGTCTCCCTCGG
CCGTCAGCGATGCGCAAGTCAACCTCGATCCCTTCGACAACGTCGAGTACGTGCAAGCCAAGGTCGAGCACGTGCTCGGC
AAGTTGGCCGTTCGGCCGGATGTCGTCATCCTCGATCCTCCGCGTGTCGGTTGCGCGCCGGAGGCGCTCACCGGTGTCCT
GATGCTGCGACCACCTCGACTCATCTACGTCTCGTGCGACCCGGCAACGCTCGCACGGGACCTGAGGAAGCTCGTCGACG
GCGGTTACCGACTACTCGATGTCACACCGCTCGACATGTTCCCACAGACCTACCACATCGAGTCGGTCGCGACACTGGAG
CTGGCCGAGGGAACGCCGTGA

Upstream 100 bases:

>100_bases
ATACCGTTGAGAGCAGCCCGCGACGCCCACTCCCGGCTGCCCACTCGGGCATGAGGTAGCCGCACTCTCCACGATTCCCT
ACACTTTCCAAGGAGGGAGG

Downstream 100 bases:

>100_bases
CGCTGGTCCTCGCCTCGGCCTCGCCGCGCCGGCGTGCGCTCTTGGCCGTACTCGGCATCCCCTTCGTCGTCGATCCCGCG
GCGATCGACGAGCCGCTCCC

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MPMGETLPTPRTLRTVPLHLEDMAYQGAAIGREDGRIVFAEYGIPGEDVLVAIEKDRKDHSLGRVIEVRTASPERVDPPC
PYFGICGGCQWQHIRYEYQLVLKQAVVRQQLRRIGKFDDPPVLPTVPSPQPYGYRNHARFSVDGKGNLGYISRPGHGYRF
LRIDRCLIMHPAINEVLRRLQERAHVKHQLMVRYGVNTGELLVHPDVSAIDPTIPSGQPAYHESLLGHRFRISASSFFQT
NTAVAERLIQLVLERIAPTGNEVVVDAYAGVGTFAAFLAPRVARVIGIEESPSAVSDAQVNLDPFDNVEYVQAKVEHVLG
KLAVRPDVVILDPPRVGCAPEALTGVLMLRPPRLIYVSCDPATLARDLRKLVDGGYRLLDVTPLDMFPQTYHIESVATLE
LAEGTP

Sequences:

>Translated_406_residues
MPMGETLPTPRTLRTVPLHLEDMAYQGAAIGREDGRIVFAEYGIPGEDVLVAIEKDRKDHSLGRVIEVRTASPERVDPPC
PYFGICGGCQWQHIRYEYQLVLKQAVVRQQLRRIGKFDDPPVLPTVPSPQPYGYRNHARFSVDGKGNLGYISRPGHGYRF
LRIDRCLIMHPAINEVLRRLQERAHVKHQLMVRYGVNTGELLVHPDVSAIDPTIPSGQPAYHESLLGHRFRISASSFFQT
NTAVAERLIQLVLERIAPTGNEVVVDAYAGVGTFAAFLAPRVARVIGIEESPSAVSDAQVNLDPFDNVEYVQAKVEHVLG
KLAVRPDVVILDPPRVGCAPEALTGVLMLRPPRLIYVSCDPATLARDLRKLVDGGYRLLDVTPLDMFPQTYHIESVATLE
LAEGTP
>Mature_405_residues
PMGETLPTPRTLRTVPLHLEDMAYQGAAIGREDGRIVFAEYGIPGEDVLVAIEKDRKDHSLGRVIEVRTASPERVDPPCP
YFGICGGCQWQHIRYEYQLVLKQAVVRQQLRRIGKFDDPPVLPTVPSPQPYGYRNHARFSVDGKGNLGYISRPGHGYRFL
RIDRCLIMHPAINEVLRRLQERAHVKHQLMVRYGVNTGELLVHPDVSAIDPTIPSGQPAYHESLLGHRFRISASSFFQTN
TAVAERLIQLVLERIAPTGNEVVVDAYAGVGTFAAFLAPRVARVIGIEESPSAVSDAQVNLDPFDNVEYVQAKVEHVLGK
LAVRPDVVILDPPRVGCAPEALTGVLMLRPPRLIYVSCDPATLARDLRKLVDGGYRLLDVTPLDMFPQTYHIESVATLEL
AEGTP

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=203, Percent_Identity=35.9605911330049, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI51173878, Length=203, Percent_Identity=35.9605911330049, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI269784678, Length=205, Percent_Identity=30.7317073170732, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI269784680, Length=205, Percent_Identity=30.7317073170732, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI269784676, Length=205, Percent_Identity=30.7317073170732, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI20149688, Length=205, Percent_Identity=30.7317073170732, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789148, Length=428, Percent_Identity=29.9065420560748, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1787083, Length=193, Percent_Identity=27.979274611399, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=25.8241758241758, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666579, Length=208, Percent_Identity=28.3653846153846, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 45065; Mature: 44934

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMGETLPTPRTLRTVPLHLEDMAYQGAAIGREDGRIVFAEYGIPGEDVLVAIEKDRKDH
CCCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCC
SLGRVIEVRTASPERVDPPCPYFGICGGCQWQHIRYEYQLVLKQAVVRQQLRRIGKFDDP
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVLPTVPSPQPYGYRNHARFSVDGKGNLGYISRPGHGYRFLRIDRCLIMHPAINEVLRRL
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCEEEEEECEEEEECHHHHHHHHHH
QERAHVKHQLMVRYGVNTGELLVHPDVSAIDPTIPSGQPAYHESLLGHRFRISASSFFQT
HHHHHHHHEEHEEECCCCCCEEECCCCHHCCCCCCCCCCHHHHHHHCCEEEECHHHHHHH
NTAVAERLIQLVLERIAPTGNEVVVDAYAGVGTFAAFLAPRVARVIGIEESPSAVSDAQV
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
NLDPFDNVEYVQAKVEHVLGKLAVRPDVVILDPPRVGCAPEALTGVLMLRPPRLIYVSCD
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCEEEEECCCEEEEEECC
PATLARDLRKLVDGGYRLLDVTPLDMFPQTYHIESVATLELAEGTP
HHHHHHHHHHHHCCCEEEEEECCHHHCCCEEEECCEEEEEECCCCC
>Mature Secondary Structure 
PMGETLPTPRTLRTVPLHLEDMAYQGAAIGREDGRIVFAEYGIPGEDVLVAIEKDRKDH
CCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCC
SLGRVIEVRTASPERVDPPCPYFGICGGCQWQHIRYEYQLVLKQAVVRQQLRRIGKFDDP
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVLPTVPSPQPYGYRNHARFSVDGKGNLGYISRPGHGYRFLRIDRCLIMHPAINEVLRRL
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCEEEEEECEEEEECHHHHHHHHHH
QERAHVKHQLMVRYGVNTGELLVHPDVSAIDPTIPSGQPAYHESLLGHRFRISASSFFQT
HHHHHHHHEEHEEECCCCCCEEECCCCHHCCCCCCCCCCHHHHHHHCCEEEECHHHHHHH
NTAVAERLIQLVLERIAPTGNEVVVDAYAGVGTFAAFLAPRVARVIGIEESPSAVSDAQV
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
NLDPFDNVEYVQAKVEHVLGKLAVRPDVVILDPPRVGCAPEALTGVLMLRPPRLIYVSCD
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCEEEEECCCEEEEEECC
PATLARDLRKLVDGGYRLLDVTPLDMFPQTYHIESVATLELAEGTP
HHHHHHHHHHHHCCCEEEEEECCHHHCCCEEEECCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]