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The map label for this gene is kdgA [H]

Identifier: 221632231

GI number: 221632231

Start: 203867

End: 204508

Strand: Reverse

Name: kdgA [H]

Synonym: trd_0197

Alternate gene names: 221632231

Gene position: 204508-203867 (Counterclockwise)

Preceding gene: 221632233

Following gene: 221632230

Centisome position: 10.21

GC content: 62.77

Gene sequence:

>642_bases
GTGAACTGGATCGAAACGTACCGGCTGATCGCCGTGATCCGGCTCGATGACTTGAGCTGTGCCGTCGAACTCGCCGAAGC
CTTACTCGAGGGCGGAGTGCGCGTGCTCGAGTTCACCTACACGAATCGTGAAGCTGGTCGTGCCATCGAACAGGTGCGGC
ATCGTTTCGATGGATCCTGCCTGGTCGGAGCAGGGACGGTCCTGGATGCAGAAACCGCGCGTCAGGCGATGTTGGCTGGG
GCTCAATTCATCGTTACCCCGACACTCCGGCGAGAAACGATCGAGATCTGCCGACGCTATTCCATCCCCAGCGTGATCGG
TGCTTTCACCCCGACCGAAATCCTCAGCGCGTGGGAATGGGGCGCTGATTACATCAAAGTCAACCCGGCCAGTCTCGCGG
GACCGAGCTATTTCAAGGACGTGCTCGCTCCCCTGCCGCAGGTGAAACTGATCCCCTCGGGTGGCGTGACACTCGAGACC
GCACCGGCTTTCCTGGCGGCCGGCGCCGTCGCCGTGGCGGTCGGCAGTCACCTTATCGATCGCACGCTGGTTGCGCGCCG
CGACTGGGCTGGATTGCGGGAACGCGCTCACCAATGGGCGGAACTGGTCGCTCACCCGGATATCGGCGTTCCGGCATCGT
GA

Upstream 100 bases:

>100_bases
ACGACGCCGAACCGTCGTGCCATCGTGATGACTCCTACTCTCCGCCGACCCGGCCCGCTACACTCGATGCGAATGGTACC
CGAGCGGAAGGGGGAGCGAC

Downstream 100 bases:

>100_bases
TCGGCGAGCGAGGCATGGCCGAAACGACGAACCACCGCATCCGGCTGTACCGCACCGAGGCGATCGTCCTGCGCCGCCGC
GACCTCGGCGAGGCCGACAG

Product: putative aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNWIETYRLIAVIRLDDLSCAVELAEALLEGGVRVLEFTYTNREAGRAIEQVRHRFDGSCLVGAGTVLDAETARQAMLAG
AQFIVTPTLRRETIEICRRYSIPSVIGAFTPTEILSAWEWGADYIKVNPASLAGPSYFKDVLAPLPQVKLIPSGGVTLET
APAFLAAGAVAVAVGSHLIDRTLVARRDWAGLRERAHQWAELVAHPDIGVPAS

Sequences:

>Translated_213_residues
MNWIETYRLIAVIRLDDLSCAVELAEALLEGGVRVLEFTYTNREAGRAIEQVRHRFDGSCLVGAGTVLDAETARQAMLAG
AQFIVTPTLRRETIEICRRYSIPSVIGAFTPTEILSAWEWGADYIKVNPASLAGPSYFKDVLAPLPQVKLIPSGGVTLET
APAFLAAGAVAVAVGSHLIDRTLVARRDWAGLRERAHQWAELVAHPDIGVPAS
>Mature_213_residues
MNWIETYRLIAVIRLDDLSCAVELAEALLEGGVRVLEFTYTNREAGRAIEQVRHRFDGSCLVGAGTVLDAETARQAMLAG
AQFIVTPTLRRETIEICRRYSIPSVIGAFTPTEILSAWEWGADYIKVNPASLAGPSYFKDVLAPLPQVKLIPSGGVTLET
APAFLAAGAVAVAVGSHLIDRTLVARRDWAGLRERAHQWAELVAHPDIGVPAS

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=179, Percent_Identity=31.2849162011173, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788156, Length=185, Percent_Identity=30.2702702702703, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23091; Mature: 23091

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWIETYRLIAVIRLDDLSCAVELAEALLEGGVRVLEFTYTNREAGRAIEQVRHRFDGSC
CCHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCE
LVGAGTVLDAETARQAMLAGAQFIVTPTLRRETIEICRRYSIPSVIGAFTPTEILSAWEW
EEECCCEECHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHC
GADYIKVNPASLAGPSYFKDVLAPLPQVKLIPSGGVTLETAPAFLAAGAVAVAVGSHLID
CCCEEEECCHHHCCHHHHHHHHCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
RTLVARRDWAGLRERAHQWAELVAHPDIGVPAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNWIETYRLIAVIRLDDLSCAVELAEALLEGGVRVLEFTYTNREAGRAIEQVRHRFDGSC
CCHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCE
LVGAGTVLDAETARQAMLAGAQFIVTPTLRRETIEICRRYSIPSVIGAFTPTEILSAWEW
EEECCCEECHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHC
GADYIKVNPASLAGPSYFKDVLAPLPQVKLIPSGGVTLETAPAFLAAGAVAVAVGSHLID
CCCEEEECCHHHCCHHHHHHHHCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHH
RTLVARRDWAGLRERAHQWAELVAHPDIGVPAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]