Click here to switch to the map view.

The map label for this gene is mdh [H]

Identifier: 221632228

GI number: 221632228

Start: 200776

End: 202062

Strand: Reverse

Name: mdh [H]

Synonym: trd_0194

Alternate gene names: 221632228

Gene position: 202062-200776 (Counterclockwise)

Preceding gene: 221632229

Following gene: 221632227

Centisome position: 10.09

GC content: 65.27

Gene sequence:

>1287_bases
ATGAGCGCGAACCTGACCCAGACCGTCATCGCCATGGACCTCGTCCCCTTCCGTTTCGGGCCCGGTGCGACCGCTGACGT
GACCGTTGAACTCGAGCGGCTCGGGGCACGCCGCGTCCTCCTCGTCACTGACCGTCATCTGCGACAGCATGGCCTGGTCG
AGCCAGTCGAGGCAGCGATCCTACGTGCTGGTGCGGAAGTGGTCGTCTTCGACCAGGTGCACATCGAGCCGACCGATCGC
TCGTTGCGCGATGCCATCGCCTTCGCCCAACGCGTCCAGCCGGACGCCTATGTTTCGCTCGGTGGTGGGAGCACGATCGA
CACAGCTAAGGCGATGAACCTCTTCACGTCCTACCCGGCTGACCTCTTCGACTATATCAACAAGCCGGTCGGTCGAGGCA
ATCCAGTTCCCGGCCCATTGAAGCCGCACCTGGCACTCCCCACGACCGCCGGTACAGGCAGCGAAACCACCGCGGTGATC
GCCCTCGAACTGACCGATCGTCGGTTGAAGACCGGTATCTCGCACCGTTTCATCCGCCCGACCATGGCGATCGTCGATCC
GCTCAATACGGTCACGTGTCCCCCGATGGTGACCGCGTTCGCCGGGCTGGATGTCCTCTGCCATGCGATCGAGTCGTACA
CAGCCAAGCCGTATCACGTCCGTCCTCGACCGGACAGCCCGACCGAGCGACCAGCCTATATCGGGTCCAACCCGGTCGCT
GACCTGTGGAGCGCACAGGCTATCCGGTGGGGTGCACGGTATCTACGGCGAGCAGTCTACAACCCGCTCGATCTGGAGGC
CCGCACCTTTATGGCGCTGGCTAGCTTGACGGCCGGTATCGGCTTCGGGAACGCCGGGGTCCACATCCCACACGCCATGT
CCTACCCGATCGCCGGGCTCGTTCGTGACTTCTTCCCGCCCAACTACGAGGTCGACGAGCCGCTCATCCCGCACGGCCTG
GCCGTCGTCGTGGGCGCTCCGGCTGGTCTCCGCTTTACAGCACCGGCCTGGCCCGAACGACACGCTGAGGTGGCCGAGAT
GCTGGGGATCGACACCCGCGGCCTGCCAGTCCGCGCAGCTGCCGAAGCGGTTGCCGATGCACTCATCCAGCTCATGCGCG
ATCTCGGCCTGCCGAGTGGTCTCGCGGAACTCGGCTTCACCGAGAGCGACATCCCCGCACTCGTCGAGGGAACACTGGCC
CAGCAGCGCATCCTGGTGAACAGCCCACGGCCAGTCGACGAGCGAGCACTGCGCGCCATCTACCGCGAGGCCATGCGGTA
CTGGTGA

Upstream 100 bases:

>100_bases
CCACCACGCTCCGTGATGGAGAGCACACGCTCCTCTTGACACCTCGTCCCTCCTGGCCGACGATGATGCCGCCTGTTGTG
TTCCGGAAGGAGCATCCGGC

Downstream 100 bases:

>100_bases
GCATCGCCCAAAGCGCGGGCGGTCCAGTCATCGGGGCAGGAGCCAGCGATCGAGCTGGGCGGGTCTCCTCAGTCATGCTG
TTCCGATGGAACCACCGACT

Product: 1,3-propanediol dehydrogenase

Products: NA

Alternate protein names: MEDH [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSANLTQTVIAMDLVPFRFGPGATADVTVELERLGARRVLLVTDRHLRQHGLVEPVEAAILRAGAEVVVFDQVHIEPTDR
SLRDAIAFAQRVQPDAYVSLGGGSTIDTAKAMNLFTSYPADLFDYINKPVGRGNPVPGPLKPHLALPTTAGTGSETTAVI
ALELTDRRLKTGISHRFIRPTMAIVDPLNTVTCPPMVTAFAGLDVLCHAIESYTAKPYHVRPRPDSPTERPAYIGSNPVA
DLWSAQAIRWGARYLRRAVYNPLDLEARTFMALASLTAGIGFGNAGVHIPHAMSYPIAGLVRDFFPPNYEVDEPLIPHGL
AVVVGAPAGLRFTAPAWPERHAEVAEMLGIDTRGLPVRAAAEAVADALIQLMRDLGLPSGLAELGFTESDIPALVEGTLA
QQRILVNSPRPVDERALRAIYREAMRYW

Sequences:

>Translated_428_residues
MSANLTQTVIAMDLVPFRFGPGATADVTVELERLGARRVLLVTDRHLRQHGLVEPVEAAILRAGAEVVVFDQVHIEPTDR
SLRDAIAFAQRVQPDAYVSLGGGSTIDTAKAMNLFTSYPADLFDYINKPVGRGNPVPGPLKPHLALPTTAGTGSETTAVI
ALELTDRRLKTGISHRFIRPTMAIVDPLNTVTCPPMVTAFAGLDVLCHAIESYTAKPYHVRPRPDSPTERPAYIGSNPVA
DLWSAQAIRWGARYLRRAVYNPLDLEARTFMALASLTAGIGFGNAGVHIPHAMSYPIAGLVRDFFPPNYEVDEPLIPHGL
AVVVGAPAGLRFTAPAWPERHAEVAEMLGIDTRGLPVRAAAEAVADALIQLMRDLGLPSGLAELGFTESDIPALVEGTLA
QQRILVNSPRPVDERALRAIYREAMRYW
>Mature_427_residues
SANLTQTVIAMDLVPFRFGPGATADVTVELERLGARRVLLVTDRHLRQHGLVEPVEAAILRAGAEVVVFDQVHIEPTDRS
LRDAIAFAQRVQPDAYVSLGGGSTIDTAKAMNLFTSYPADLFDYINKPVGRGNPVPGPLKPHLALPTTAGTGSETTAVIA
LELTDRRLKTGISHRFIRPTMAIVDPLNTVTCPPMVTAFAGLDVLCHAIESYTAKPYHVRPRPDSPTERPAYIGSNPVAD
LWSAQAIRWGARYLRRAVYNPLDLEARTFMALASLTAGIGFGNAGVHIPHAMSYPIAGLVRDFFPPNYEVDEPLIPHGLA
VVVGAPAGLRFTAPAWPERHAEVAEMLGIDTRGLPVRAAAEAVADALIQLMRDLGLPSGLAELGFTESDIPALVEGTLAQ
QRILVNSPRPVDERALRAIYREAMRYW

Specific function: Fucose metabolism; fourth step. [C]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=429, Percent_Identity=48.2517482517483, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1789163, Length=381, Percent_Identity=29.9212598425197, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI48994951, Length=407, Percent_Identity=27.5184275184275, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1787493, Length=390, Percent_Identity=28.7179487179487, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082107, Length=406, Percent_Identity=28.0788177339901, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789386, Length=200, Percent_Identity=26.5, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17537053, Length=436, Percent_Identity=41.9724770642202, Blast_Score=348, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6321181, Length=411, Percent_Identity=26.2773722627737, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24657991, Length=419, Percent_Identity=47.4940334128878, Blast_Score=375, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.244 [H]

Molecular weight: Translated: 46373; Mature: 46242

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANLTQTVIAMDLVPFRFGPGATADVTVELERLGARRVLLVTDRHLRQHGLVEPVEAAI
CCCCHHHHHHHHHHHCEECCCCCCCEEEEEHHHCCCCEEEEEECHHHHHCCCCCHHHHHH
LRAGAEVVVFDQVHIEPTDRSLRDAIAFAQRVQPDAYVSLGGGSTIDTAKAMNLFTSYPA
HHCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCH
DLFDYINKPVGRGNPVPGPLKPHLALPTTAGTGSETTAVIALELTDRRLKTGISHRFIRP
HHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEEECHHHHHHCCHHHHHHH
TMAIVDPLNTVTCPPMVTAFAGLDVLCHAIESYTAKPYHVRPRPDSPTERPAYIGSNPVA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHH
DLWSAQAIRWGARYLRRAVYNPLDLEARTFMALASLTAGIGFGNAGVHIPHAMSYPIAGL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHHH
VRDFFPPNYEVDEPLIPHGLAVVVGAPAGLRFTAPAWPERHAEVAEMLGIDTRGLPVRAA
HHHHCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCCCCCHHHH
AEAVADALIQLMRDLGLPSGLAELGFTESDIPALVEGTLAQQRILVNSPRPVDERALRAI
HHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHH
YREAMRYW
HHHHHHCC
>Mature Secondary Structure 
SANLTQTVIAMDLVPFRFGPGATADVTVELERLGARRVLLVTDRHLRQHGLVEPVEAAI
CCCHHHHHHHHHHHCEECCCCCCCEEEEEHHHCCCCEEEEEECHHHHHCCCCCHHHHHH
LRAGAEVVVFDQVHIEPTDRSLRDAIAFAQRVQPDAYVSLGGGSTIDTAKAMNLFTSYPA
HHCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCH
DLFDYINKPVGRGNPVPGPLKPHLALPTTAGTGSETTAVIALELTDRRLKTGISHRFIRP
HHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEEECHHHHHHCCHHHHHHH
TMAIVDPLNTVTCPPMVTAFAGLDVLCHAIESYTAKPYHVRPRPDSPTERPAYIGSNPVA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHH
DLWSAQAIRWGARYLRRAVYNPLDLEARTFMALASLTAGIGFGNAGVHIPHAMSYPIAGL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHHH
VRDFFPPNYEVDEPLIPHGLAVVVGAPAGLRFTAPAWPERHAEVAEMLGIDTRGLPVRAA
HHHHCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCCCCCHHHH
AEAVADALIQLMRDLGLPSGLAELGFTESDIPALVEGTLAQQRILVNSPRPVDERALRAI
HHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHH
YREAMRYW
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644761; 1995642 [H]