Click here to switch to the map view.

The map label for this gene is punA [H]

Identifier: 221632229

GI number: 221632229

Start: 202202

End: 203071

Strand: Reverse

Name: punA [H]

Synonym: trd_0195

Alternate gene names: 221632229

Gene position: 203071-202202 (Counterclockwise)

Preceding gene: 221632230

Following gene: 221632228

Centisome position: 10.14

GC content: 65.63

Gene sequence:

>870_bases
ATGGACATCCTCCATCCCTGGCAGGAACGAGCAGCTGAGCGAGCGCAGATACTCGCCCAGCGTTATCACCACACTCCTGA
CGCGCTCATCGTCCTCGGCTCCGGGCTGGCGACTCTCCCTGAAGACGCGCATGTCGTGGCACGCGTCCCGCTCGACGAAC
TCACTGCGATCGCTGCCCCGGCTGTCACCGGTCATCCACGCGAGTTGGTGCTGGCAGAGTGGAATGGGCACCCGATCTGG
TTTTGCCTCGGTCGTTATCACCTCTACCAGGGTCTCTCGGCTGCAGAAGCCGCTGCGCCGATCGCCCTCCTCGCCCACCT
GCCTGGTCCGATCGTCATCCTGACCAATGCCGCCGGCTCCTTGCATCCGACCTTCGCACCGGGCGATCTCGTGCTGATCC
GGGATCACTTCTTCGTCCCCGGTTTGGCCGGTCACCATCCGCTGATTCCACCGGCGGGACGGGTTCGCTTCTTCAGTCTG
CGCGATGCCTACGATCGCCACCTCCGCGCGGTAGCTCGCGCTGCTGCGGCCCGACTCGGCCTGGAACTCCCGGAGGGAAC
GTATGCCATGGTCGCTGGGCCGACCTTCGAGACGGCAGCCGAACTTCGCTGGTTGCGAGCCGCCGGGGCTGATCTGGTCG
GCATGTCGACCATCCCCGAAGTGATCCTAGCGCGGGCACTCGGGTTACGCGTCCTGGCCATCTCCGTCGTGACCAACTTG
GCGATCCCAGAAGAGCCAGCGATCCCGACTCACGAAGAGGTGGCGGCCATCGGGGAACAGGCCCGGCCGCGCCTCCATCG
ACTGCTCGGCTTCTTCCTCGACCAACTGGCCGAGGAAAGCGATGCCGTCACGGGCCGCGAGCCTGTCTGA

Upstream 100 bases:

>100_bases
CAGATCACGAGTGCCCTCCAGGCAGCGCTCCAGGCGCACATCGGCGACATCGCTGCCTCCGCCGCCGTGGCGACACTCTT
GCAGGAAGAGGGGCACGATG

Downstream 100 bases:

>100_bases
GCACCGTCCAAGCAGTGATCGACAGACCCGGGCGCGGCACCACCACGCTCCGTGATGGAGAGCACACGCTCCTCTTGACA
CCTCGTCCCTCCTGGCCGAC

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDILHPWQERAAERAQILAQRYHHTPDALIVLGSGLATLPEDAHVVARVPLDELTAIAAPAVTGHPRELVLAEWNGHPIW
FCLGRYHLYQGLSAAEAAAPIALLAHLPGPIVILTNAAGSLHPTFAPGDLVLIRDHFFVPGLAGHHPLIPPAGRVRFFSL
RDAYDRHLRAVARAAAARLGLELPEGTYAMVAGPTFETAAELRWLRAAGADLVGMSTIPEVILARALGLRVLAISVVTNL
AIPEEPAIPTHEEVAAIGEQARPRLHRLLGFFLDQLAEESDAVTGREPV

Sequences:

>Translated_289_residues
MDILHPWQERAAERAQILAQRYHHTPDALIVLGSGLATLPEDAHVVARVPLDELTAIAAPAVTGHPRELVLAEWNGHPIW
FCLGRYHLYQGLSAAEAAAPIALLAHLPGPIVILTNAAGSLHPTFAPGDLVLIRDHFFVPGLAGHHPLIPPAGRVRFFSL
RDAYDRHLRAVARAAAARLGLELPEGTYAMVAGPTFETAAELRWLRAAGADLVGMSTIPEVILARALGLRVLAISVVTNL
AIPEEPAIPTHEEVAAIGEQARPRLHRLLGFFLDQLAEESDAVTGREPV
>Mature_289_residues
MDILHPWQERAAERAQILAQRYHHTPDALIVLGSGLATLPEDAHVVARVPLDELTAIAAPAVTGHPRELVLAEWNGHPIW
FCLGRYHLYQGLSAAEAAAPIALLAHLPGPIVILTNAAGSLHPTFAPGDLVLIRDHFFVPGLAGHHPLIPPAGRVRFFSL
RDAYDRHLRAVARAAAARLGLELPEGTYAMVAGPTFETAAELRWLRAAGADLVGMSTIPEVILARALGLRVLAISVVTNL
AIPEEPAIPTHEEVAAIGEQARPRLHRLLGFFLDQLAEESDAVTGREPV

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=272, Percent_Identity=38.9705882352941, Blast_Score=182, Evalue=2e-46,
Organism=Escherichia coli, GI1788746, Length=259, Percent_Identity=35.1351351351351, Blast_Score=144, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17541190, Length=257, Percent_Identity=34.2412451361868, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6323238, Length=310, Percent_Identity=37.4193548387097, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI45552885, Length=283, Percent_Identity=31.095406360424, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45552887, Length=283, Percent_Identity=31.095406360424, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24656090, Length=281, Percent_Identity=30.9608540925267, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24656093, Length=281, Percent_Identity=30.9608540925267, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24762376, Length=248, Percent_Identity=29.8387096774194, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 31108; Mature: 31108

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILHPWQERAAERAQILAQRYHHTPDALIVLGSGLATLPEDAHVVARVPLDELTAIAAP
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCHHHHHHHHCC
AVTGHPRELVLAEWNGHPIWFCLGRYHLYQGLSAAEAAAPIALLAHLPGPIVILTNAAGS
CCCCCCCEEEEEECCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCC
LHPTFAPGDLVLIRDHFFVPGLAGHHPLIPPAGRVRFFSLRDAYDRHLRAVARAAAARLG
CCCCCCCCCEEEEECCEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHC
LELPEGTYAMVAGPTFETAAELRWLRAAGADLVGMSTIPEVILARALGLRVLAISVVTNL
CCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
AIPEEPAIPTHEEVAAIGEQARPRLHRLLGFFLDQLAEESDAVTGREPV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDILHPWQERAAERAQILAQRYHHTPDALIVLGSGLATLPEDAHVVARVPLDELTAIAAP
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCHHHHHHHHCC
AVTGHPRELVLAEWNGHPIWFCLGRYHLYQGLSAAEAAAPIALLAHLPGPIVILTNAAGS
CCCCCCCEEEEEECCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCC
LHPTFAPGDLVLIRDHFFVPGLAGHHPLIPPAGRVRFFSLRDAYDRHLRAVARAAAARLG
CCCCCCCCCEEEEECCEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHC
LELPEGTYAMVAGPTFETAAELRWLRAAGADLVGMSTIPEVILARALGLRVLAISVVTNL
CCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
AIPEEPAIPTHEEVAAIGEQARPRLHRLLGFFLDQLAEESDAVTGREPV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]