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The map label for this gene is epsJ [H]

Identifier: 221232501

GI number: 221232501

Start: 1709130

End: 1710035

Strand: Reverse

Name: epsJ [H]

Synonym: SPN23F_17730

Alternate gene names: 221232501

Gene position: 1710035-1709130 (Counterclockwise)

Preceding gene: 221232502

Following gene: 221232500

Centisome position: 76.98

GC content: 34.11

Gene sequence:

>906_bases
ATGAGTGAATTAATTAGTGTTGTGGTACCGATATACAATACGGGAAAATATTTAGTGGAGTGTGTCGAGCATATTCTGAA
GCAAACCTATCAAAATATAGAAATTATTTTAGTTGATGACGGTTCTACGGATAATTCTGGGGAAATTTGTGATGCTTTTA
TGATGCAAGATAATCGTGTGCGAGTATTGCATCAAGAAAATAAGGGGGGGGCAGCACAAGCTAAAAATATGGGGATTAGT
GTAGCTAAGGGAGAGTACATCACGATTGTTGATTCAGATGATATCGTAAAAGAAAATATGATTGAAACTCTTTATCAGCA
AGTCCAAGAAAAGGATGCAGATGTTGTTATAGGGAATTACTATAATTATGACGAAAGTGACGGGAATTTTTATTTTTATG
TAACAGGGAAAGATTTTTGCGTCGAAGAATTAGCTATACAAGAAATTATGAACCGTCAAGCAGGAGATTGGAAATTCAAT
AGCTCGGCCTTTATATTGCCGACATTTAAGTTGATTAAAAAAGAATTATTCAATGAAGTTCACTTTTCAAATGGTCGCCG
CTTTGATGATGAAGCAACTATGCATCGCTTTTATCTTTTAGCCTCTAAAATCGTCTTTATAAACGATAATCTCTATCTGT
ATAGAAGACGTTCAGGAAGCATCATGAGAACGGAATTTGATCTTTCCTGGGCAAGAGATATTGTTGAAGTGTTTTCTAAG
AAAATATCGGATTGTGTCTTGGCTGGTTTGGATGTCTCCGTTCTGCGTATTCGATTTGTCAATCTTTTAAAAGATTATAA
GCAAACTTTAGAATACCATCAATTAACAGATACTGAGGAATATAAAGATATTTGTTTCAGATTAAAGTTGTTTTTTGATG
CAGAACAAAGAAATGGTAAAAGTTGA

Upstream 100 bases:

>100_bases
TCACACGTAAAAGTATGGATGATGGACTCTATGACGGTATTTTTTCTGTGGAGAGACCAGATGATTTAGTGGATAGAATG
AAGAATATAGAGATAGAGTA

Downstream 100 bases:

>100_bases
AATAAAAGAATTGTTATTTACCATATCACAAACAATGAAGGTGAGGGGCGTGTTTTATGACTAAGATTTATTCGTCTATA
ACAGTAAAAAAAGGATTATT

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTDNSGEICDAFMMQDNRVRVLHQENKGGAAQAKNMGIS
VAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFN
SSAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVEVFSK
KISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDAEQRNGKS

Sequences:

>Translated_301_residues
MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTDNSGEICDAFMMQDNRVRVLHQENKGGAAQAKNMGIS
VAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFN
SSAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVEVFSK
KISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDAEQRNGKS
>Mature_300_residues
SELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTDNSGEICDAFMMQDNRVRVLHQENKGGAAQAKNMGISV
AKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFNS
SAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVEVFSKK
ISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDAEQRNGKS

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790044, Length=115, Percent_Identity=45.2173913043478, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787259, Length=92, Percent_Identity=38.0434782608696, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788372, Length=111, Percent_Identity=32.4324324324324, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 35011; Mature: 34880

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTDNSGEICDAFMMQDNRV
CCCHHEEHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHCCCCCE
RVLHQENKGGAAQAKNMGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
EEEEECCCCCCCHHHCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECC
YNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFNEV
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEHHHHHHHHHHHHHH
HFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVEVFSK
HCCCCCCCCHHHHHHHHHHHHCEEEEEECCEEEEEECCCCEEEEECCHHHHHHHHHHHHH
KISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDAEQRNGK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECHHCCCC
S
C
>Mature Secondary Structure 
SELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTDNSGEICDAFMMQDNRV
CCHHEEHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHCCCCCE
RVLHQENKGGAAQAKNMGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
EEEEECCCCCCCHHHCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECC
YNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFNEV
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEHHHHHHHHHHHHHH
HFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVEVFSK
HCCCCCCCCHHHHHHHHHHHHCEEEEEECCEEEEEECCCCEEEEECCHHHHHHHHHHHHH
KISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDAEQRNGK
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECHHCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]