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The map label for this gene is insK [H]

Identifier: 221232009

GI number: 221232009

Start: 1194869

End: 1195708

Strand: Reverse

Name: insK [H]

Synonym: SPN23F_12260

Alternate gene names: 221232009

Gene position: 1195708-1194869 (Counterclockwise)

Preceding gene: 221232010

Following gene: 221232007

Centisome position: 53.83

GC content: 38.45

Gene sequence:

>840_bases
ATGGTTTCAGGAGGATTTCGACTAGATTTTCTTCTTGAAACAGCACGTTTAGCTCGCTCGACTTACTATTATCAGTTGAA
ACAACTGGATGGGGTTGATAAAGATAAAGAGATTAAAACCGAAATTCAGGGCATTGATAATGAACATAAAGGCAACTATG
GCTATCGTCGAATCCATTTAGAACTAAGGAATCGTGGTTTTGTGGTCAATCATAAGAAAGTTCAACGGTTGATGAGAATT
CTTGGTTTAACGGCTCGAATTCGCCGTAAACGGAAGTATTCTTCCTACCAAGGAGAGATTGGCAAGAAAGCAGAGAATCT
CATTCAACGCCAGTTCGAAGCATCGAGGCCAATGGAAAAGTGCTATACAGATGTGACAGAGTTTGCCATTCCAAATAGCA
CACAGAAACTCTATTTATCACCAGTTTTAGATGGTTTTAACAGTGAAATCATTGCTTATCATCTTTCTACTTCGCCAAAC
TTAGAACAAGTGAAGAGTATGTTAGAACAGGCCTTTACAGAGAAATACTATGAGAATACGATTCTCCATAGTGACCAAGG
CTGGCAATACCAACACGATTCTTATCATCGGTTCCTAGAGAGTAAGGAAATTCAAGCATCCATGTCACGCAAGGGCAACA
GCCCAGACAACGGCATGATGGAATCTTTCTTTGGCATTTTAAAATCCGAAATGTTTTATGGCTATGAGAAGAACTTTAGA
TCTTTAGAAAACCTTGAACAAGCTATTGTGGACTACATTGATTACTACAACAACAAACGAATTAAGGTAAAACTAAAAGG
ACTTAGTTCTGTGCAGTACAGAACTAAATCCTTTGGATAA

Upstream 100 bases:

>100_bases
ATGAACGCTTACGGACTGAGGTGGCCTACCTAAAAAAGTTAAAAGAGTTAGAGGAAAGGGACGAAGCCCTAGAGCGAGAA
AGGCAGAGACAGTTAGAGAA

Downstream 100 bases:

>100_bases
ATTATTTGTCTAATTTTTTGGGGTCAGTACAGTATTAAATACCATGATCTTATGCGTTTTATAATAAAAAATCTGTTCTT
GTTTTATTTTTAGAAAATTT

Product: IS861 transposase Orf2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRI
LGLTARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPN
LEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKEIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFR
SLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG

Sequences:

>Translated_279_residues
MVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRI
LGLTARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPN
LEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKEIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFR
SLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
>Mature_279_residues
MVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRI
LGLTARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPN
LEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKEIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFR
SLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=224, Percent_Identity=31.6964285714286, Blast_Score=104, Evalue=9e-23,
Organism=Escherichia coli, GI1789981, Length=274, Percent_Identity=50, Blast_Score=264, Evalue=5e-72,
Organism=Escherichia coli, GI1788405, Length=280, Percent_Identity=29.2857142857143, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787263, Length=280, Percent_Identity=29.2857142857143, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786753, Length=280, Percent_Identity=29.2857142857143, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786570, Length=280, Percent_Identity=29.2857142857143, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786490, Length=280, Percent_Identity=29.2857142857143, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32859; Mature: 32859

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEE
ELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEK
EECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHH
CYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENT
HHHHHHHHCCCCCCCEEEEHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
ILHSDQGWQYQHDSYHRFLESKEIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFR
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHH
SLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCC
>Mature Secondary Structure
MVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEE
ELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEK
EECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHH
CYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENT
HHHHHHHHCCCCCCCEEEEHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
ILHSDQGWQYQHDSYHRFLESKEIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFR
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHH
SLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
HHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]