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The map label for this gene is nanA [H]

Identifier: 221232004

GI number: 221232004

Start: 1189320

End: 1190237

Strand: Reverse

Name: nanA [H]

Synonym: SPN23F_12210

Alternate gene names: 221232004

Gene position: 1190237-1189320 (Counterclockwise)

Preceding gene: 221232005

Following gene: 221232003

Centisome position: 53.58

GC content: 35.4

Gene sequence:

>918_bases
ATGAAAGATTTAACTAAATACAAAGGCGTTATTCCTGCATTTTATGCTTGCTATGATGAAAATGGTGAAATTAGCCAAGA
ACGTGTAAAATCTCTGGTGCAATATTTCATTGACAAAGGTGTAAAAGGTATCTATGTAAATGGTTCTTCAGGTGAATGTA
TTTACCAAAGTGTAGAAGATCGTAAACAAATTATTGAAGCTGTTATGGAAGTTGCTAAAGGTAAATTAACAGTTATCAAC
CATGTTGCATGTAATAACACGAAAGATAGTATTGAATTGGCAAAACATTCAGAAAGTGTTGGAGTCGATGCTATTGCTGC
TATCCCACCTATTTACTTCAAATTGCCAGAATATTCAATTGCAGCATATTGGAATGCAATGAGTGAAGCTGCGCCAAATA
CAGATTTTATCATTTATAATATTCCACAATTGGCAGGGGTTGCGTTGACTGGTAGTTTGTATGCAACAATGCGTCAAAAT
CCTCGTGTGATTGGAGTTAAAAATTCTTCTATGCCTGTACAAGATATTCAAATGTTTGTAGCTGCAGGTGGAGAAGATTA
CATTGTATTCAATGGTCCAGATGAACAATATCTTGGTGGTCGCTTGATGGGAGCAGAAGCTGGTATTGGTGGTACTTATG
GCGTTATGCCAGATTTGTTCTTGAAATTGGAAAGTTTGATTCAAGAACGAGATTTAGATACTGCTAAAAAACTTCAATAT
GCTATCAATGAAGTTATCTATAAGATGATATCAGGTAAGGCAAATATGTATGCTGTAGCAAAAGAGGTTTTGCGTCTAAA
TGAAAAACTTGATTTAGGTTCTGTTCGTCAACCCTTAGAAGCATTGGCAGAAGGTGACTTGGAAGTTGCAAAACAAGCAG
CAGAACTTATTCAACAAGCACGAAAAGAATTTTTATAA

Upstream 100 bases:

>100_bases
TTACGCAGTGGTTGTTGGAGGAGCTATCACTCGTCCAAAAGAAATTGCTCAGCGATTTATTAATGTCATCAAATAAAAAA
CAATATAAAGGAGAAATCTG

Downstream 100 bases:

>100_bases
TAAATAAAATCAATATGGAGATATGTTATGGGCACAACAGGATTTACAATAATTGACTTAATTATCTTGATTGTTTATTT
ACTTGCGGTGTTGGTTGCAG

Product: N-acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase 1; N-acetylneuraminic acid aldolase 1; Sialate lyase 1; Sialic acid aldolase 1; Sialic acid lyase 1 [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGIYVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVIN
HVACNNTKDSIELAKHSESVGVDAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPDLFLKLESLIQERDLDTAKKLQY
AINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVRQPLEALAEGDLEVAKQAAELIQQARKEFL

Sequences:

>Translated_305_residues
MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGIYVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVIN
HVACNNTKDSIELAKHSESVGVDAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPDLFLKLESLIQERDLDTAKKLQY
AINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVRQPLEALAEGDLEVAKQAAELIQQARKEFL
>Mature_305_residues
MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGIYVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVIN
HVACNNTKDSIELAKHSESVGVDAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPDLFLKLESLIQERDLDTAKKLQY
AINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVRQPLEALAEGDLEVAKQAAELIQQARKEFL

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=281, Percent_Identity=30.2491103202847, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI31543060, Length=239, Percent_Identity=20.5020920502092, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789620, Length=297, Percent_Identity=32.3232323232323, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87082415, Length=240, Percent_Identity=25.4166666666667, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786463, Length=240, Percent_Identity=26.25, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788823, Length=211, Percent_Identity=21.8009478672986, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 33573; Mature: 33573

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGIYVNGSSGECIYQSVED
CCCHHHHCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
RKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGVDAIAAIPPIYFKLPEYSI
HHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHCCCCEEECCCCHH
AAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQNPRVIGVKNSSMPVQDIQMFV
HHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
AAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPDLFLKLESLIQERDLDTAKKLQY
HCCCCCEEEECCCCCHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVRQPLEALAEGDLEVAKQAAELIQQA
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RKEFL
HHHCC
>Mature Secondary Structure
MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGIYVNGSSGECIYQSVED
CCCHHHHCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
RKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGVDAIAAIPPIYFKLPEYSI
HHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHCCCCEEECCCCHH
AAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQNPRVIGVKNSSMPVQDIQMFV
HHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
AAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPDLFLKLESLIQERDLDTAKKLQY
HCCCCCEEEECCCCCHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVRQPLEALAEGDLEVAKQAAELIQQA
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RKEFL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]