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The map label for this gene is purA

Identifier: 220933142

GI number: 220933142

Start: 2522818

End: 2524104

Strand: Reverse

Name: purA

Synonym: Hore_23100

Alternate gene names: 220933142

Gene position: 2524104-2522818 (Counterclockwise)

Preceding gene: 220933143

Following gene: 220933141

Centisome position: 97.9

GC content: 44.99

Gene sequence:

>1287_bases
ATGGGTAATAAAGTTGTGGTTGGAACCCAGTGGGGAGATGAAGGAAAAGGTAAAATTACAGATATGCTAGCCAGAACAGC
CGATGTAGTAGTCCGCTACGGAGGAGGTAATAATGCCGGACATACTGTAATTGTAGATGGTAAGAAATTTGAACTCCACT
TAATACCTTCCGGAATCCTGTACCCGGAAAAGGTTAATGTTATCGGAAATGGAGTTGTAGTTGATCCTGAAGCCCTGGTA
GAGGAAATGAATATGTTAAAAGAAGAGGGCATTTCTCTTAATAACCTGTATGTCAGTGAAACAGCCCATGTCATCATGCC
CTACCACAGGCTTTTAGATAAACTGGAAGAAAAGAGGAAGGGTGACGGCAAGATCGGTACTACAGGCCGTGGTATCGGAC
CGGCCTATACCGATAAAGTTGCCAGGCGTGGCCTCAGGGTTATAGATCTTCTCGATGAAGATACTTTTTATGAAAAACTT
AAATTAGCCCTGGATTACCAGAATCTACTCCTTGAAAAAGTATATCAGGTTGAACCCATGGATATTAAAGAGATAATGGC
TCAATATAAAGAATATATCGAAGTGTTAAGGCCCCATGTAACCAACACTTCACTGCTTCTGGATGAGGCAATTAAGGAGG
ATAAGAAGATATTCTTCGAGGGGGCCCAGGGAACCCTTCTTGATATTGATTATGGGACTTATCCATATGTTACTTCTTCA
AATCCTACTGCCGGAGGGGTATGTACAGGTACAGGAGTTGGCCCGGTCTGGATTGATGACGTTATCGGAGTGGTAAAGGC
CTACCTGACAAGGGTGGGCGAAGGCCCCTTCCCGACTGAACTCAATAATGATATCGGTGATAAACTGAGGGATAAAGGAC
ATGAATACGGAGTGACCACCGGACGCCCCCGGAGGTGTGGCTGGTTAGACATCCCCATCTTAAAACATGCTGTCCGGGTT
AATGGACTGACAGAACTGGCTTTAACAAAACTGGATGTCCTGTCAGGCCTCAGGGAGGTCAAGGTCTGTACCGGATATCG
TTATGGAGACCGGCTTATAGAAGAATTCCCCGGAAATATTGATATTCTTTCCGGATGTGAGCCAGTTTATGAAGTGCTGC
CGGGCTGGGATGATGATATTACCGGTGTCAGGGAGTATGGAGATTTGCCTGAAAACGCCAGAAGGTATGTGAAGTTAATA
GAGGATATGGTTAAGGTTCCGGTAACCATTATCGGGGTTGGTCCTGGTCGAAAAGAAGCGATAAGGAGAGAGAAGGAGGT
TAATTAA

Upstream 100 bases:

>100_bases
TTAAAAAATGAAAAAACTAGTTTTTAATAACCTTACTTTAGATAGACTATATATTAATGAGAGGAGGGAGCTTAAACCAT
CAGGGTTTAAGCGCTTATCG

Downstream 100 bases:

>100_bases
TGAAAATTTTAGTTGTAGGAAGTGGGGGTAGGGAGCATGCCCTGGTCTGGAAGTTATACCAGAGTGAACGTGTTGAGCGG
ATATTTGTGGCCCCCGGTAA

Product: Adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MGNKVVVGTQWGDEGKGKITDMLARTADVVVRYGGGNNAGHTVIVDGKKFELHLIPSGILYPEKVNVIGNGVVVDPEALV
EEMNMLKEEGISLNNLYVSETAHVIMPYHRLLDKLEEKRKGDGKIGTTGRGIGPAYTDKVARRGLRVIDLLDEDTFYEKL
KLALDYQNLLLEKVYQVEPMDIKEIMAQYKEYIEVLRPHVTNTSLLLDEAIKEDKKIFFEGAQGTLLDIDYGTYPYVTSS
NPTAGGVCTGTGVGPVWIDDVIGVVKAYLTRVGEGPFPTELNNDIGDKLRDKGHEYGVTTGRPRRCGWLDIPILKHAVRV
NGLTELALTKLDVLSGLREVKVCTGYRYGDRLIEEFPGNIDILSGCEPVYEVLPGWDDDITGVREYGDLPENARRYVKLI
EDMVKVPVTIIGVGPGRKEAIRREKEVN

Sequences:

>Translated_428_residues
MGNKVVVGTQWGDEGKGKITDMLARTADVVVRYGGGNNAGHTVIVDGKKFELHLIPSGILYPEKVNVIGNGVVVDPEALV
EEMNMLKEEGISLNNLYVSETAHVIMPYHRLLDKLEEKRKGDGKIGTTGRGIGPAYTDKVARRGLRVIDLLDEDTFYEKL
KLALDYQNLLLEKVYQVEPMDIKEIMAQYKEYIEVLRPHVTNTSLLLDEAIKEDKKIFFEGAQGTLLDIDYGTYPYVTSS
NPTAGGVCTGTGVGPVWIDDVIGVVKAYLTRVGEGPFPTELNNDIGDKLRDKGHEYGVTTGRPRRCGWLDIPILKHAVRV
NGLTELALTKLDVLSGLREVKVCTGYRYGDRLIEEFPGNIDILSGCEPVYEVLPGWDDDITGVREYGDLPENARRYVKLI
EDMVKVPVTIIGVGPGRKEAIRREKEVN
>Mature_427_residues
GNKVVVGTQWGDEGKGKITDMLARTADVVVRYGGGNNAGHTVIVDGKKFELHLIPSGILYPEKVNVIGNGVVVDPEALVE
EMNMLKEEGISLNNLYVSETAHVIMPYHRLLDKLEEKRKGDGKIGTTGRGIGPAYTDKVARRGLRVIDLLDEDTFYEKLK
LALDYQNLLLEKVYQVEPMDIKEIMAQYKEYIEVLRPHVTNTSLLLDEAIKEDKKIFFEGAQGTLLDIDYGTYPYVTSSN
PTAGGVCTGTGVGPVWIDDVIGVVKAYLTRVGEGPFPTELNNDIGDKLRDKGHEYGVTTGRPRRCGWLDIPILKHAVRVN
GLTELALTKLDVLSGLREVKVCTGYRYGDRLIEEFPGNIDILSGCEPVYEVLPGWDDDITGVREYGDLPENARRYVKLIE
DMVKVPVTIIGVGPGRKEAIRREKEVN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=49.2957746478873, Blast_Score=387, Evalue=1e-108,
Organism=Homo sapiens, GI34577063, Length=433, Percent_Identity=47.5750577367206, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=48.4848484848485, Blast_Score=348, Evalue=4e-96,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=52.8301886792453, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=44.8837209302326, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=44.8837209302326, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=47.906976744186, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=49.2924528301887, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_HALOH (B8D1A3)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002510050.1
- GeneID:   7314193
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_23100
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47489; Mature: 47358

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKVVVGTQWGDEGKGKITDMLARTADVVVRYGGGNNAGHTVIVDGKKFELHLIPSGIL
CCCEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEEECCEEEEEECCCCCC
YPEKVNVIGNGVVVDPEALVEEMNMLKEEGISLNNLYVSETAHVIMPYHRLLDKLEEKRK
CCCEEEEEECCEEECHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHC
GDGKIGTTGRGIGPAYTDKVARRGLRVIDLLDEDTFYEKLKLALDYQNLLLEKVYQVEPM
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DIKEIMAQYKEYIEVLRPHVTNTSLLLDEAIKEDKKIFFEGAQGTLLDIDYGTYPYVTSS
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCEEECC
NPTAGGVCTGTGVGPVWIDDVIGVVKAYLTRVGEGPFPTELNNDIGDKLRDKGHEYGVTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
GRPRRCGWLDIPILKHAVRVNGLTELALTKLDVLSGLREVKVCTGYRYGDRLIEEFPGNI
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
DILSGCEPVYEVLPGWDDDITGVREYGDLPENARRYVKLIEDMVKVPVTIIGVGPGRKEA
EEECCCCHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHH
IRREKEVN
HHHHHCCC
>Mature Secondary Structure 
GNKVVVGTQWGDEGKGKITDMLARTADVVVRYGGGNNAGHTVIVDGKKFELHLIPSGIL
CCEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEEECCEEEEEECCCCCC
YPEKVNVIGNGVVVDPEALVEEMNMLKEEGISLNNLYVSETAHVIMPYHRLLDKLEEKRK
CCCEEEEEECCEEECHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHC
GDGKIGTTGRGIGPAYTDKVARRGLRVIDLLDEDTFYEKLKLALDYQNLLLEKVYQVEPM
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
DIKEIMAQYKEYIEVLRPHVTNTSLLLDEAIKEDKKIFFEGAQGTLLDIDYGTYPYVTSS
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCEEECC
NPTAGGVCTGTGVGPVWIDDVIGVVKAYLTRVGEGPFPTELNNDIGDKLRDKGHEYGVTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
GRPRRCGWLDIPILKHAVRVNGLTELALTKLDVLSGLREVKVCTGYRYGDRLIEEFPGNI
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
DILSGCEPVYEVLPGWDDDITGVREYGDLPENARRYVKLIEDMVKVPVTIIGVGPGRKEA
EEECCCCHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHH
IRREKEVN
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA