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The map label for this gene is purB [H]

Identifier: 220933143

GI number: 220933143

Start: 2524233

End: 2525528

Strand: Reverse

Name: purB [H]

Synonym: Hore_23110

Alternate gene names: 220933143

Gene position: 2525528-2524233 (Counterclockwise)

Preceding gene: 220933148

Following gene: 220933142

Centisome position: 97.96

GC content: 45.83

Gene sequence:

>1296_bases
TTGCTTAAACGCTACAGTCTACCTGAGATGGAATACGTCTGGAGTGAAGAGAGTAAGTATAATAAATGGCTGGAGATTGA
AATTGCTGCCTGCCAGGCCTGGTCCCGATTGGGGCGAATACCGGGCGAATCTGTTAAGAAAATTAAGAAAAATGTAGAGT
TAAATGTTGACAGGATTAAGGAAATAGAAAAGGTAACCCGTCATGATATGCTGGCTTTTGTGGAAGGTATAAATGAAACC
CTGGGAGAGGAATCCAAGTATATACATATGGGATTAACCTCTTCAGATGTCAAGGATACGGCTTTAGCAATGCAGCTTAA
GGATTCCTGTCAGTTGATTTTAAAAGACCTTAAAAACCTGAAAGATGTTCTGAAAATCCAGGCTTTAAATCATAAAGATA
CGGTGATGATCGGGCGAACTCACGGGGTTCACGCCGAGCCGGTAACCTGGGGGTTAAAGCTGGCTATCTGGTATGCCGAG
ATTGAAAGGCATATAGAAAGGATGGAAAGGGTTAAAGAGATGGTCAGTGTCGGGAAAATATCCGGAGCGGTTGGCACCTA
TGCCAATGTTGACCCCCGGGTTGAGGAATATGTCTGCGAGGAACTGGGCTTAAACCCGGCCCCGGTTAGTAGCCAGATAC
TGCAGCGGGACCGCCATGCCGATTACCTCAATAACCTGGCGTTAATTGCCGCTTCCCTGGAGAAATTCGCCACCGAAATC
CGTAACCTGCAGAGGACCGATATTCTTGAGGTGGAAGAGGGATTCCGGAAAGGGCAGAAAGGCTCTTCAGCCATGCCCCA
TAAAAAGAATCCAATTATATGTGAACGGATTAGCGGCCTGGCCCGGGTGGTCAGGGGTAATGTGGTCCCCGGGTTGGAGG
ATATTACCCTGTGGCATGAGCGGGATTTAACCCATTCTTCAGTGGAAAGGATAATTTTACCCGACAGTTCTATTCTGGTT
GATTATATGTTGAATAAATTCAGGATGGTCCTTGAGGAGCTTGTTGTTAATGATGAGCGGATGAAGGAAAACCTGAATAC
CACCCATGGGCTAATTTTTTCCCAGAAAGTAATGCTGGCGCTGGTGGATAAGGGATTGTCCCGTGAGGAGGCTTATCGCC
TGGCCCAGCGTAATGCCCTGAAAGCATGGGAGACCGGTGAAGACTACCAACAGTTACTTAAAAAAGACCGGGAAGTGTCA
ACCTATCTTAATGGTGAAGAGATCGACCATATCTTTGATTACTATTCATTTATTCAGGAGATTGACCATATCTTCAAAAA
ACTTGGACTTGAATAG

Upstream 100 bases:

>100_bases
ATTAAATACTTCATACTTACCACCTAATATTATAAAGCATCTTTTAATAAAGAAAAAGGCTTAAAAAAGGATTAAATTTA
ACCAGAAAGGAAGATACCCT

Downstream 100 bases:

>100_bases
GGCCGGTGTTTCAAGTGCCCTTCAGGAATTAAAAAATGAAAAAACTAGTTTTTAATAACCTTACTTTAGATAGACTATAT
ATTAATGAGAGGAGGGAGCT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MLKRYSLPEMEYVWSEESKYNKWLEIEIAACQAWSRLGRIPGESVKKIKKNVELNVDRIKEIEKVTRHDMLAFVEGINET
LGEESKYIHMGLTSSDVKDTALAMQLKDSCQLILKDLKNLKDVLKIQALNHKDTVMIGRTHGVHAEPVTWGLKLAIWYAE
IERHIERMERVKEMVSVGKISGAVGTYANVDPRVEEYVCEELGLNPAPVSSQILQRDRHADYLNNLALIAASLEKFATEI
RNLQRTDILEVEEGFRKGQKGSSAMPHKKNPIICERISGLARVVRGNVVPGLEDITLWHERDLTHSSVERIILPDSSILV
DYMLNKFRMVLEELVVNDERMKENLNTTHGLIFSQKVMLALVDKGLSREEAYRLAQRNALKAWETGEDYQQLLKKDREVS
TYLNGEEIDHIFDYYSFIQEIDHIFKKLGLE

Sequences:

>Translated_431_residues
MLKRYSLPEMEYVWSEESKYNKWLEIEIAACQAWSRLGRIPGESVKKIKKNVELNVDRIKEIEKVTRHDMLAFVEGINET
LGEESKYIHMGLTSSDVKDTALAMQLKDSCQLILKDLKNLKDVLKIQALNHKDTVMIGRTHGVHAEPVTWGLKLAIWYAE
IERHIERMERVKEMVSVGKISGAVGTYANVDPRVEEYVCEELGLNPAPVSSQILQRDRHADYLNNLALIAASLEKFATEI
RNLQRTDILEVEEGFRKGQKGSSAMPHKKNPIICERISGLARVVRGNVVPGLEDITLWHERDLTHSSVERIILPDSSILV
DYMLNKFRMVLEELVVNDERMKENLNTTHGLIFSQKVMLALVDKGLSREEAYRLAQRNALKAWETGEDYQQLLKKDREVS
TYLNGEEIDHIFDYYSFIQEIDHIFKKLGLE
>Mature_431_residues
MLKRYSLPEMEYVWSEESKYNKWLEIEIAACQAWSRLGRIPGESVKKIKKNVELNVDRIKEIEKVTRHDMLAFVEGINET
LGEESKYIHMGLTSSDVKDTALAMQLKDSCQLILKDLKNLKDVLKIQALNHKDTVMIGRTHGVHAEPVTWGLKLAIWYAE
IERHIERMERVKEMVSVGKISGAVGTYANVDPRVEEYVCEELGLNPAPVSSQILQRDRHADYLNNLALIAASLEKFATEI
RNLQRTDILEVEEGFRKGQKGSSAMPHKKNPIICERISGLARVVRGNVVPGLEDITLWHERDLTHSSVERIILPDSSILV
DYMLNKFRMVLEELVVNDERMKENLNTTHGLIFSQKVMLALVDKGLSREEAYRLAQRNALKAWETGEDYQQLLKKDREVS
TYLNGEEIDHIFDYYSFIQEIDHIFKKLGLE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=438, Percent_Identity=28.9954337899543, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI183227688, Length=384, Percent_Identity=29.6875, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1787376, Length=351, Percent_Identity=28.7749287749288, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787896, Length=210, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790398, Length=392, Percent_Identity=24.234693877551, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508577, Length=388, Percent_Identity=27.5773195876289, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564234, Length=335, Percent_Identity=28.0597014925373, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17553882, Length=277, Percent_Identity=27.4368231046931, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32565146, Length=274, Percent_Identity=27.7372262773723, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=449, Percent_Identity=28.2850779510022, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24647570, Length=434, Percent_Identity=25.5760368663594, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI221473854, Length=428, Percent_Identity=24.2990654205607, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78706858, Length=428, Percent_Identity=24.2990654205607, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78710009, Length=281, Percent_Identity=28.8256227758007, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49604; Mature: 49604

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRYSLPEMEYVWSEESKYNKWLEIEIAACQAWSRLGRIPGESVKKIKKNVELNVDRIK
CCCCCCCCCHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHH
EIEKVTRHDMLAFVEGINETLGEESKYIHMGLTSSDVKDTALAMQLKDSCQLILKDLKNL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KDVLKIQALNHKDTVMIGRTHGVHAEPVTWGLKLAIWYAEIERHIERMERVKEMVSVGKI
HHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANVDPRVEEYVCEELGLNPAPVSSQILQRDRHADYLNNLALIAASLEKFATEI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDILEVEEGFRKGQKGSSAMPHKKNPIICERISGLARVVRGNVVPGLEDITLWHE
HHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RDLTHSSVERIILPDSSILVDYMLNKFRMVLEELVVNDERMKENLNTTHGLIFSQKVMLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHH
LVDKGLSREEAYRLAQRNALKAWETGEDYQQLLKKDREVSTYLNGEEIDHIFDYYSFIQE
HHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHH
IDHIFKKLGLE
HHHHHHHHCCC
>Mature Secondary Structure
MLKRYSLPEMEYVWSEESKYNKWLEIEIAACQAWSRLGRIPGESVKKIKKNVELNVDRIK
CCCCCCCCCHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHH
EIEKVTRHDMLAFVEGINETLGEESKYIHMGLTSSDVKDTALAMQLKDSCQLILKDLKNL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KDVLKIQALNHKDTVMIGRTHGVHAEPVTWGLKLAIWYAEIERHIERMERVKEMVSVGKI
HHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANVDPRVEEYVCEELGLNPAPVSSQILQRDRHADYLNNLALIAASLEKFATEI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDILEVEEGFRKGQKGSSAMPHKKNPIICERISGLARVVRGNVVPGLEDITLWHE
HHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RDLTHSSVERIILPDSSILVDYMLNKFRMVLEELVVNDERMKENLNTTHGLIFSQKVMLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHH
LVDKGLSREEAYRLAQRNALKAWETGEDYQQLLKKDREVSTYLNGEEIDHIFDYYSFIQE
HHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHH
IDHIFKKLGLE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]