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The map label for this gene is purB [H]

Identifier: 220933049

GI number: 220933049

Start: 2414172

End: 2415560

Strand: Reverse

Name: purB [H]

Synonym: Hore_22170

Alternate gene names: 220933049

Gene position: 2415560-2414172 (Counterclockwise)

Preceding gene: 220933050

Following gene: 220933048

Centisome position: 93.69

GC content: 44.28

Gene sequence:

>1389_bases
ATGGAACGAAATATTTTTGAAAATATCTGTCCACTTGATCACCGATATTCTTTAAAAAAAGATCTGTTTAATGACCTCGG
CAAATACTTTTCGGAAAAGGCTACAATTTATTTTCAGGCTAGAGTTGAGCTGGCCCTGGTTAAGGCCCTGGCCGGGCGTG
GGGTATGTAGTTTTGATGTGGTTAAGGAGGTTGAAGAGGCTGTTAAAGGGATAAAGGTAGGGGAAGTATATGAAGAAGAG
GCCCGGACCAGGCATAATATAAGGGCTCTTGTTAACTGTATTCGAAAGAAAATAAGTGCTGAGGCCCGTCCCTTTGTCCA
TCTTTCGGCAACTTCCTTTGATATTGTAGATACGGCCAATAGCCTTCGATATAAAGAAGCTACAGAAAAATTGATTGTAC
CGGCACTGGAGCGGTTACTGGAGCAGTGGCTTAATCTGGCCGAACAGGAAAGGGATACACTCCAGATTGGCCGTACCCAC
GGGCAGCATGCTGAACCCCTTACCTTTGGGCTGGTTATGGCTTATTATGCCAGCCGGCTTCATGAGCGGATTGTTAACCT
GGAGAAGGTGGTTTCCAGCCTGTGTGGTAAGATGGCCGGGGCGGTAGGAGCCTATAATGCCAGCAGTCTATTTTTTGAAG
ATCCGGAAGAATTCGAAAAAGAAGTATTAAAGGAACTGGGCCTGAAGCCCGCTCCCGTTTCCACCCAGATAGTCCCTCCC
GAATATATGACCGACTATGTCCATTCCCTGATTACTGTTTTTGGGGTTCTGGCTGACTTCAGTGATGATATGAGGCACCT
GCAGAGGACAGAAATAGGGGAAACAGGGGAGTATTTTGCTAAAGACCAGGTCGGTTCTTCGACCATGCCCCATAAAAGAA
ACCCCATTAATTATGAGAATGTTAAAAGCATGTGGAAAACCTTTATGCCCCGGATGGTGACCATGTATCTGGATCAGCTT
TCTGAACACCAGAGAGATCTAACCAATTCAGCCTCAGGTCGTTTTATTGCTGAAATAGCCTCAGGATTAATGATGACGGT
AGACCGGCTGTATAAAGTATCTTCACGGATGAAAATTAATTCCCGGAAAATGAAGAATAATTTTAACTTAAGTAAAAATA
TGATAATTGCTGAACCACTCTATATATTCCTGGCAGCTGCTGGCCACCCCGATGCCCATGAATATGTAAGAAAACTTACC
CTGAAGGCTGATAAAGAGGGTAAGGATCTGGTTGAATTAATTAAAGCCGACAAAGAAATTGAAACCTACCTGGCTAAACT
CGATAAACGCCAGAAGGATATTCTCTATAATCCGGAGCAGTATACCGGTATTGCCCGGGAAAGGACTGAAGAGATTGTGA
CTAAAATCAGGAAGGAGAGGGGTTTGTAA

Upstream 100 bases:

>100_bases
ATTTATAATGGCTATCGTTCATGGTTGTCTGGTAAGGATTTTCAGACAATATTATTTCATTCTTATCATATTACAGGTTT
TCAGGGAAAGGGAGGGTATA

Downstream 100 bases:

>100_bases
TGTTAACAAAACTGGAAATGCTTTATGAAGGAAAGGCTAAAAAGGTTTATCGGACCGAGGACAAAGACAGGGTTATTATT
GAGTATAAGGATGATGCTAC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MERNIFENICPLDHRYSLKKDLFNDLGKYFSEKATIYFQARVELALVKALAGRGVCSFDVVKEVEEAVKGIKVGEVYEEE
ARTRHNIRALVNCIRKKISAEARPFVHLSATSFDIVDTANSLRYKEATEKLIVPALERLLEQWLNLAEQERDTLQIGRTH
GQHAEPLTFGLVMAYYASRLHERIVNLEKVVSSLCGKMAGAVGAYNASSLFFEDPEEFEKEVLKELGLKPAPVSTQIVPP
EYMTDYVHSLITVFGVLADFSDDMRHLQRTEIGETGEYFAKDQVGSSTMPHKRNPINYENVKSMWKTFMPRMVTMYLDQL
SEHQRDLTNSASGRFIAEIASGLMMTVDRLYKVSSRMKINSRKMKNNFNLSKNMIIAEPLYIFLAAAGHPDAHEYVRKLT
LKADKEGKDLVELIKADKEIETYLAKLDKRQKDILYNPEQYTGIARERTEEIVTKIRKERGL

Sequences:

>Translated_462_residues
MERNIFENICPLDHRYSLKKDLFNDLGKYFSEKATIYFQARVELALVKALAGRGVCSFDVVKEVEEAVKGIKVGEVYEEE
ARTRHNIRALVNCIRKKISAEARPFVHLSATSFDIVDTANSLRYKEATEKLIVPALERLLEQWLNLAEQERDTLQIGRTH
GQHAEPLTFGLVMAYYASRLHERIVNLEKVVSSLCGKMAGAVGAYNASSLFFEDPEEFEKEVLKELGLKPAPVSTQIVPP
EYMTDYVHSLITVFGVLADFSDDMRHLQRTEIGETGEYFAKDQVGSSTMPHKRNPINYENVKSMWKTFMPRMVTMYLDQL
SEHQRDLTNSASGRFIAEIASGLMMTVDRLYKVSSRMKINSRKMKNNFNLSKNMIIAEPLYIFLAAAGHPDAHEYVRKLT
LKADKEGKDLVELIKADKEIETYLAKLDKRQKDILYNPEQYTGIARERTEEIVTKIRKERGL
>Mature_462_residues
MERNIFENICPLDHRYSLKKDLFNDLGKYFSEKATIYFQARVELALVKALAGRGVCSFDVVKEVEEAVKGIKVGEVYEEE
ARTRHNIRALVNCIRKKISAEARPFVHLSATSFDIVDTANSLRYKEATEKLIVPALERLLEQWLNLAEQERDTLQIGRTH
GQHAEPLTFGLVMAYYASRLHERIVNLEKVVSSLCGKMAGAVGAYNASSLFFEDPEEFEKEVLKELGLKPAPVSTQIVPP
EYMTDYVHSLITVFGVLADFSDDMRHLQRTEIGETGEYFAKDQVGSSTMPHKRNPINYENVKSMWKTFMPRMVTMYLDQL
SEHQRDLTNSASGRFIAEIASGLMMTVDRLYKVSSRMKINSRKMKNNFNLSKNMIIAEPLYIFLAAAGHPDAHEYVRKLT
LKADKEGKDLVELIKADKEIETYLAKLDKRQKDILYNPEQYTGIARERTEEIVTKIRKERGL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=470, Percent_Identity=25.3191489361702, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI183227688, Length=388, Percent_Identity=25.2577319587629, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1787376, Length=435, Percent_Identity=28.0459770114943, Blast_Score=142, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17508577, Length=471, Percent_Identity=24.6284501061571, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32564234, Length=341, Percent_Identity=26.099706744868, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17553882, Length=225, Percent_Identity=28.4444444444444, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=475, Percent_Identity=24, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24647570, Length=469, Percent_Identity=24.0938166311301, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 52894; Mature: 52894

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERNIFENICPLDHRYSLKKDLFNDLGKYFSEKATIYFQARVELALVKALAGRGVCSFDV
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCHHHH
VKEVEEAVKGIKVGEVYEEEARTRHNIRALVNCIRKKISAEARPFVHLSATSFDIVDTAN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCHH
SLRYKEATEKLIVPALERLLEQWLNLAEQERDTLQIGRTHGQHAEPLTFGLVMAYYASRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
HERIVNLEKVVSSLCGKMAGAVGAYNASSLFFEDPEEFEKEVLKELGLKPAPVSTQIVPP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
EYMTDYVHSLITVFGVLADFSDDMRHLQRTEIGETGEYFAKDQVGSSTMPHKRNPINYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHH
VKSMWKTFMPRMVTMYLDQLSEHQRDLTNSASGRFIAEIASGLMMTVDRLYKVSSRMKIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SRKMKNNFNLSKNMIIAEPLYIFLAAAGHPDAHEYVRKLTLKADKEGKDLVELIKADKEI
HHHHHCCCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ETYLAKLDKRQKDILYNPEQYTGIARERTEEIVTKIRKERGL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERNIFENICPLDHRYSLKKDLFNDLGKYFSEKATIYFQARVELALVKALAGRGVCSFDV
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCHHHH
VKEVEEAVKGIKVGEVYEEEARTRHNIRALVNCIRKKISAEARPFVHLSATSFDIVDTAN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCHH
SLRYKEATEKLIVPALERLLEQWLNLAEQERDTLQIGRTHGQHAEPLTFGLVMAYYASRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
HERIVNLEKVVSSLCGKMAGAVGAYNASSLFFEDPEEFEKEVLKELGLKPAPVSTQIVPP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
EYMTDYVHSLITVFGVLADFSDDMRHLQRTEIGETGEYFAKDQVGSSTMPHKRNPINYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHH
VKSMWKTFMPRMVTMYLDQLSEHQRDLTNSASGRFIAEIASGLMMTVDRLYKVSSRMKIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SRKMKNNFNLSKNMIIAEPLYIFLAAAGHPDAHEYVRKLTLKADKEGKDLVELIKADKEI
HHHHHCCCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ETYLAKLDKRQKDILYNPEQYTGIARERTEEIVTKIRKERGL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]